TY  - CONF
AU  - Rakić, Mina
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
AU  - Jelić, Mihailo
AU  - Lapčević, Simona
AU  - Savić Veselinović, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5496
AB  - Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Lead induces sex and development related differential Mtn gene expression in two Drosophila species
SP  - 471
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5496
ER  - 

@conference{
author = "Rakić, Mina and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija and Jelić, Mihailo and Lapčević, Simona and Savić Veselinović, Marija",
year = "2022",
abstract = "Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Lead induces sex and development related differential Mtn gene expression in two Drosophila species",
pages = "471",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5496"
}

Rakić, M., Patenković, A., Stamenković-Radak, M., Tanasković, M., Jelić, M., Lapčević, S.,& Savić Veselinović, M.. (2022). Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
European Society for Evolutionary Biology., 471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496

Rakić M, Patenković A, Stamenković-Radak M, Tanasković M, Jelić M, Lapčević S, Savić Veselinović M. Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .

Rakić, Mina, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, Jelić, Mihailo, Lapčević, Simona, Savić Veselinović, Marija, "Lead induces sex and development related differential Mtn gene expression in two Drosophila species" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):471,
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .