TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Mačukanović-Jocić, Marina P
AU  - Milinčić, Danijel D.
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Gligorijević, Nevenka N.
AU  - Jarić, Snežana
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200069
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35213785
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4941
AB  - The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.
T2  - Chemistry & Biodiversity
T1  - Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.
IS  - 4
VL  - 19
DO  - 10.1002/cbdv.202200069
SP  - e202200069
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Mačukanović-Jocić, Marina P and Milinčić, Danijel D. and Petrović, Jovana and Gašić, Uroš and Gligorijević, Nevenka N. and Jarić, Snežana and Soković, Marina and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.",
journal = "Chemistry & Biodiversity",
title = "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.",
number = "4",
volume = "19",
doi = "10.1002/cbdv.202200069",
pages = "e202200069"
}

Kostić, A. Ž., Mačukanović-Jocić, M. P., Milinčić, D. D., Petrović, J., Gašić, U., Gligorijević, N. N., Jarić, S., Soković, M., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity, 19(4), e202200069.
https://doi.org/10.1002/cbdv.202200069

Kostić AŽ, Mačukanović-Jocić MP, Milinčić DD, Petrović J, Gašić U, Gligorijević NN, Jarić S, Soković M, Tešić ŽL, Pešić MB. Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity. 2022;19(4):e202200069.
doi:10.1002/cbdv.202200069 .

Kostić, Aleksandar Ž., Mačukanović-Jocić, Marina P, Milinčić, Danijel D., Petrović, Jovana, Gašić, Uroš, Gligorijević, Nevenka N., Jarić, Snežana, Soković, Marina, Tešić, Živoslav Lj., Pešić, Mirjana B., "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents." in Chemistry & Biodiversity, 19, no. 4 (2022):e202200069,
https://doi.org/10.1002/cbdv.202200069 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Todorović, Slađana
AU  - Gligorijević, Nevenka N
AU  - Janković, Radmila N
AU  - Živković, Suzana
AU  - Ristić, Mihailo S.
AU  - Radulović, Sinisa S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1171
AB  - Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. The leaves secrete labdane type diterpenes that exhibit considerable antibacterial and cytotoxic activities. However there are no data about implementation of in vitro culture of C. creticus in order to obtain applicable production of labdane diterpenes. We successfully established in vitro culture of shoots and roots on solid or liquid MS (Murashige and Skoog) medium without addition of plant growth regulators. GC/FID and GC/MS detections were used to carry out characterization of ethanol extracts of shoots and roots from in vitro culture. Labdane diterpenes were the most abundant compounds in shoot extract, but absent from the root extract. Total phenolic and flavonoid contents of C. cretins shoot and root ethanol extracts were also determined. In order to evaluate possible cytotoxic activity of extracts, sulforhodamine B (SRB) assay was performed on five human cancer cell lines. Only shoot extract had cytotoxic activity on HeLa (cervix), MDA-MB-453 (breast) and FemX (melanoma) cancer cells with IC50 reaching 80.83 mu g/ml (HeLa), 76.18 mu g/ml (MDA-MB-453), and 87.52 mu g/ml (FemX) respectively. This is the first report of cytotoxic effect of labdane diterpenes from Cistus on HeLa cells. (C) 2012 Elsevier B.V. All rights reserved.
T2  - Industrial Crops and Products
T1  - Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp creticus L. on human cancer cell lines
IS  - null
VL  - 38
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1171
ER  - 

@article{
author = "Skorić, Marijana and Todorović, Slađana and Gligorijević, Nevenka N and Janković, Radmila N and Živković, Suzana and Ristić, Mihailo S. and Radulović, Sinisa S",
year = "2012",
abstract = "Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. The leaves secrete labdane type diterpenes that exhibit considerable antibacterial and cytotoxic activities. However there are no data about implementation of in vitro culture of C. creticus in order to obtain applicable production of labdane diterpenes. We successfully established in vitro culture of shoots and roots on solid or liquid MS (Murashige and Skoog) medium without addition of plant growth regulators. GC/FID and GC/MS detections were used to carry out characterization of ethanol extracts of shoots and roots from in vitro culture. Labdane diterpenes were the most abundant compounds in shoot extract, but absent from the root extract. Total phenolic and flavonoid contents of C. cretins shoot and root ethanol extracts were also determined. In order to evaluate possible cytotoxic activity of extracts, sulforhodamine B (SRB) assay was performed on five human cancer cell lines. Only shoot extract had cytotoxic activity on HeLa (cervix), MDA-MB-453 (breast) and FemX (melanoma) cancer cells with IC50 reaching 80.83 mu g/ml (HeLa), 76.18 mu g/ml (MDA-MB-453), and 87.52 mu g/ml (FemX) respectively. This is the first report of cytotoxic effect of labdane diterpenes from Cistus on HeLa cells. (C) 2012 Elsevier B.V. All rights reserved.",
journal = "Industrial Crops and Products",
title = "Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp creticus L. on human cancer cell lines",
number = "null",
volume = "38",
pages = "159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1171"
}

Skorić, M., Todorović, S., Gligorijević, N. N., Janković, R. N., Živković, S., Ristić, M. S.,& Radulović, S. S.. (2012). Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp creticus L. on human cancer cell lines. in Industrial Crops and Products, 38(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1171

Skorić M, Todorović S, Gligorijević NN, Janković RN, Živković S, Ristić MS, Radulović SS. Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp creticus L. on human cancer cell lines. in Industrial Crops and Products. 2012;38(null):null-159.
https://hdl.handle.net/21.15107/rcub_ibiss_1171 .

Skorić, Marijana, Todorović, Slađana, Gligorijević, Nevenka N, Janković, Radmila N, Živković, Suzana, Ristić, Mihailo S., Radulović, Sinisa S, "Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp creticus L. on human cancer cell lines" in Industrial Crops and Products, 38, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1171 .