TY  - JOUR
AU  - De Tommasi, Edoardo
AU  - Rea, Ilaria
AU  - Ferrara, Maria Antonietta
AU  - De Stefano, Luca
AU  - De Stefano, Mario
AU  - Al-Handal, Adil Y.
AU  - Stamenković, Marija
AU  - Wulff, Angela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6625
AB  - Ordered, quasi-ordered, and even disordered nanostructures can be identified as constituent components of several protists, plants and animals, making possible an efficient manipulation of light for intra- and inter- species communication, camouflage, or for the enhancement of primary production. Diatoms are ubiquitous unicellular microalgae inhabiting all the aquatic environments on Earth. They developed, through tens of millions of years of evolution, ultrastructured silica cell walls, the frustules, able to handle optical radiation through multiple diffractive, refractive, and wave-guiding processes, possibly at the basis of their high photosynthetic efficiency. In this study, we employed a range of imaging, spectroscopic and numerical techniques (including transmission imaging, digital holography, photoluminescence spectroscopy, and numerical simulations based on wide-angle beam propagation method) to identify and describe different mechanisms by which Pleurosigma strigosum frustules can modulate optical radiation of different spectral content. Finally, we correlated the optical response of the frustule to the interaction with light in living, individual cells within their aquatic environment following various irradiation treatments. The obtained results demonstrate the favorable transmission of photosynthetic active radiation inside the cell compared to potentially detrimental ultraviolet radiation.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Multiple-pathways light modulation in Pleurosigma strigosum bi-raphid diatom
VL  - 14
DO  - 10.1038/s41598-024-56206-y
SP  - 6476
ER  - 

@article{
author = "De Tommasi, Edoardo and Rea, Ilaria and Ferrara, Maria Antonietta and De Stefano, Luca and De Stefano, Mario and Al-Handal, Adil Y. and Stamenković, Marija and Wulff, Angela",
year = "2024",
abstract = "Ordered, quasi-ordered, and even disordered nanostructures can be identified as constituent components of several protists, plants and animals, making possible an efficient manipulation of light for intra- and inter- species communication, camouflage, or for the enhancement of primary production. Diatoms are ubiquitous unicellular microalgae inhabiting all the aquatic environments on Earth. They developed, through tens of millions of years of evolution, ultrastructured silica cell walls, the frustules, able to handle optical radiation through multiple diffractive, refractive, and wave-guiding processes, possibly at the basis of their high photosynthetic efficiency. In this study, we employed a range of imaging, spectroscopic and numerical techniques (including transmission imaging, digital holography, photoluminescence spectroscopy, and numerical simulations based on wide-angle beam propagation method) to identify and describe different mechanisms by which Pleurosigma strigosum frustules can modulate optical radiation of different spectral content. Finally, we correlated the optical response of the frustule to the interaction with light in living, individual cells within their aquatic environment following various irradiation treatments. The obtained results demonstrate the favorable transmission of photosynthetic active radiation inside the cell compared to potentially detrimental ultraviolet radiation.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Multiple-pathways light modulation in Pleurosigma strigosum bi-raphid diatom",
volume = "14",
doi = "10.1038/s41598-024-56206-y",
pages = "6476"
}

De Tommasi, E., Rea, I., Ferrara, M. A., De Stefano, L., De Stefano, M., Al-Handal, A. Y., Stamenković, M.,& Wulff, A.. (2024). Multiple-pathways light modulation in Pleurosigma strigosum bi-raphid diatom. in Scientific Reports
Springer Nature., 14, 6476.
https://doi.org/10.1038/s41598-024-56206-y

De Tommasi E, Rea I, Ferrara MA, De Stefano L, De Stefano M, Al-Handal AY, Stamenković M, Wulff A. Multiple-pathways light modulation in Pleurosigma strigosum bi-raphid diatom. in Scientific Reports. 2024;14:6476.
doi:10.1038/s41598-024-56206-y .

De Tommasi, Edoardo, Rea, Ilaria, Ferrara, Maria Antonietta, De Stefano, Luca, De Stefano, Mario, Al-Handal, Adil Y., Stamenković, Marija, Wulff, Angela, "Multiple-pathways light modulation in Pleurosigma strigosum bi-raphid diatom" in Scientific Reports, 14 (2024):6476,
https://doi.org/10.1038/s41598-024-56206-y . .

TY  - JOUR
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Wulff, Angela
AU  - Henley, W.
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1111/jpy.13150
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4189
AB  - Although desmids typically inhabit freshwater environments characterized by low amounts of nutrients and low salinity, several desmid species have been recorded in eutrophic waters, indicating their adaptation to elevated pollution and conductivity. This study aimed to determine whether desmids could be used for remediation of moderately saline aquaculture wastewater (AWW) from a fish farm situated in the southeast of Sweden. Fourteen desmid strains isolated from different climates (tropical to polar) and trophic conditions (oligotrophic to eutrophic) were cultivated in diluted AWW and we estimated their growth rates, biomass, nutrient removal efficiency, chlorophyll fluorescence parameters and cellular C, N and P quotas. Despite being grown at moderate salinity, unfavourable N:P ratio, and relatively low light/temperature regime the eutrophic strains, Cosmarium humile, Cosmarium laeve and a meso-oligotrophic species Cosmarium impressulum, completely absorbed nitrate and phosphate from AWW media after 7 d, indicating their potential for remediation of fish effluents in colder climates. These species, along with the typical eutrophic species, Cosmarium meneghinii and Staurastrum chaetoceras, had biomass in the range 0.45-1.19 g · L −1 while maximum growth rates ranged from 0.36 to 0.51 · d −1 , similar to published rates for several fast-growing green microalgae cultivated in various AWW types. Tropical desmids had distinctly high values of saturating irradiance (I k > 1,000 µmol photons · m −2 · s −1), and, along with eutrophic desmids, had high potential electron transport (rETR max > 155 rel. units). Hence, the desmids studied demonstrated inherent photophysiological responses corresponding to their climate and trophic origin under the suboptimal growth conditions.
PB  - Wiley
T2  - Journal of Phycology
T1  - Cultivation and Photophysiological Characteristics of Desmids in Moderately Saline Aquaculture Wastewater
IS  - 3
VL  - 57
DO  - 10.1111/jpy.13150
SP  - 726
EP  - 741
ER  - 

@article{
author = "Stamenković, Marija and Steinwall, Elin and Wulff, Angela and Henley, W.",
year = "2021",
abstract = "Although desmids typically inhabit freshwater environments characterized by low amounts of nutrients and low salinity, several desmid species have been recorded in eutrophic waters, indicating their adaptation to elevated pollution and conductivity. This study aimed to determine whether desmids could be used for remediation of moderately saline aquaculture wastewater (AWW) from a fish farm situated in the southeast of Sweden. Fourteen desmid strains isolated from different climates (tropical to polar) and trophic conditions (oligotrophic to eutrophic) were cultivated in diluted AWW and we estimated their growth rates, biomass, nutrient removal efficiency, chlorophyll fluorescence parameters and cellular C, N and P quotas. Despite being grown at moderate salinity, unfavourable N:P ratio, and relatively low light/temperature regime the eutrophic strains, Cosmarium humile, Cosmarium laeve and a meso-oligotrophic species Cosmarium impressulum, completely absorbed nitrate and phosphate from AWW media after 7 d, indicating their potential for remediation of fish effluents in colder climates. These species, along with the typical eutrophic species, Cosmarium meneghinii and Staurastrum chaetoceras, had biomass in the range 0.45-1.19 g · L −1 while maximum growth rates ranged from 0.36 to 0.51 · d −1 , similar to published rates for several fast-growing green microalgae cultivated in various AWW types. Tropical desmids had distinctly high values of saturating irradiance (I k > 1,000 µmol photons · m −2 · s −1), and, along with eutrophic desmids, had high potential electron transport (rETR max > 155 rel. units). Hence, the desmids studied demonstrated inherent photophysiological responses corresponding to their climate and trophic origin under the suboptimal growth conditions.",
publisher = "Wiley",
journal = "Journal of Phycology",
title = "Cultivation and Photophysiological Characteristics of Desmids in Moderately Saline Aquaculture Wastewater",
number = "3",
volume = "57",
doi = "10.1111/jpy.13150",
pages = "726-741"
}

Stamenković, M., Steinwall, E., Wulff, A.,& Henley, W.. (2021). Cultivation and Photophysiological Characteristics of Desmids in Moderately Saline Aquaculture Wastewater. in Journal of Phycology
Wiley., 57(3), 726-741.
https://doi.org/10.1111/jpy.13150

Stamenković M, Steinwall E, Wulff A, Henley W. Cultivation and Photophysiological Characteristics of Desmids in Moderately Saline Aquaculture Wastewater. in Journal of Phycology. 2021;57(3):726-741.
doi:10.1111/jpy.13150 .

Stamenković, Marija, Steinwall, Elin, Wulff, Angela, Henley, W., "Cultivation and Photophysiological Characteristics of Desmids in Moderately Saline Aquaculture Wastewater" in Journal of Phycology, 57, no. 3 (2021):726-741,
https://doi.org/10.1111/jpy.13150 . .

TY  - JOUR
AU  - De Tommasi, Edoardo
AU  - Rea, Ilaria
AU  - Ferrara, Maria Antonietta
AU  - De Stefano, Luca
AU  - De Stefano, Mario
AU  - Al-Handal, Adil Y.
AU  - Stamenković, Marija
AU  - Wulff, Angela
PY  - 2021
UR  - https://www.mdpi.com/2079-4991/11/11/2855
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4652
AB  - Several species of diatoms, unicellular microalgae which constitute the main component of phytoplankton, are characterized by an impressive photosynthetic efficiency while presenting a noticeable tolerance versus exposure to detrimental UV radiation (UVR). In particular, the growth rate of the araphid diatom Ctenophora pulchella is not significantly affected by harsh treatments with UVR, even in absence of detectable, specific UV-absorbing pigments and even if it is not able to avoid high UV exposure by motility. In this work we applied a multi-disciplinary approach involving numerical computation, photonics, and biological parameters in order to investigate the possible role of the frustule, micro- and nano-patterned silica shell which encloses the cell, in the ability of C. pulchella to efficiently collect photosynthetic active radiation (PAR) and to simultaneously screen the protoplasm from UVR. The characterization of the photonic properties of the frustule has been accompanied by in vivo experiments conducted in water in order to investigate its function as optical coupler between light and plastids.
PB  - Basel: MDPI
T2  - Nanomaterials
T1  - Underwater Light Manipulation by the Benthic Diatom Ctenophora pulchella: From PAR Efficient Collection to UVR Screening
IS  - 11
VL  - 11
DO  - 10.3390/nano11112855
SP  - 2855
ER  - 

@article{
author = "De Tommasi, Edoardo and Rea, Ilaria and Ferrara, Maria Antonietta and De Stefano, Luca and De Stefano, Mario and Al-Handal, Adil Y. and Stamenković, Marija and Wulff, Angela",
year = "2021",
abstract = "Several species of diatoms, unicellular microalgae which constitute the main component of phytoplankton, are characterized by an impressive photosynthetic efficiency while presenting a noticeable tolerance versus exposure to detrimental UV radiation (UVR). In particular, the growth rate of the araphid diatom Ctenophora pulchella is not significantly affected by harsh treatments with UVR, even in absence of detectable, specific UV-absorbing pigments and even if it is not able to avoid high UV exposure by motility. In this work we applied a multi-disciplinary approach involving numerical computation, photonics, and biological parameters in order to investigate the possible role of the frustule, micro- and nano-patterned silica shell which encloses the cell, in the ability of C. pulchella to efficiently collect photosynthetic active radiation (PAR) and to simultaneously screen the protoplasm from UVR. The characterization of the photonic properties of the frustule has been accompanied by in vivo experiments conducted in water in order to investigate its function as optical coupler between light and plastids.",
publisher = "Basel: MDPI",
journal = "Nanomaterials",
title = "Underwater Light Manipulation by the Benthic Diatom Ctenophora pulchella: From PAR Efficient Collection to UVR Screening",
number = "11",
volume = "11",
doi = "10.3390/nano11112855",
pages = "2855"
}

De Tommasi, E., Rea, I., Ferrara, M. A., De Stefano, L., De Stefano, M., Al-Handal, A. Y., Stamenković, M.,& Wulff, A.. (2021). Underwater Light Manipulation by the Benthic Diatom Ctenophora pulchella: From PAR Efficient Collection to UVR Screening. in Nanomaterials
Basel: MDPI., 11(11), 2855.
https://doi.org/10.3390/nano11112855

De Tommasi E, Rea I, Ferrara MA, De Stefano L, De Stefano M, Al-Handal AY, Stamenković M, Wulff A. Underwater Light Manipulation by the Benthic Diatom Ctenophora pulchella: From PAR Efficient Collection to UVR Screening. in Nanomaterials. 2021;11(11):2855.
doi:10.3390/nano11112855 .

De Tommasi, Edoardo, Rea, Ilaria, Ferrara, Maria Antonietta, De Stefano, Luca, De Stefano, Mario, Al-Handal, Adil Y., Stamenković, Marija, Wulff, Angela, "Underwater Light Manipulation by the Benthic Diatom Ctenophora pulchella: From PAR Efficient Collection to UVR Screening" in Nanomaterials, 11, no. 11 (2021):2855,
https://doi.org/10.3390/nano11112855 . .

TY  - JOUR
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders K.
AU  - Wulff, Angela
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0031942219304558?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3529
AB  - Desmids (Zygnematophyceae) are a group of poorly studied green microalgae. The aim of the present study was to identify fatty acids (FAs) that could be used as biomarkers in desmids in general, and to determine FAs as traits within different ecophysiological desmid groups. FA profiles of 29 desmid strains were determined and analysed with respect to their geographic origin, trophic preference and age of cultivation. It appeared that merely FAs present in relatively large proportions such as palmitic, linoleic, α-linolenic and hexadecatrienoic acids could be used as biomarkers for reliable categorization of this microalgal group. Linear discriminant analysis applied to three a priori defined groups of desmids, revealed clear strain-specific characteristics regarding FA distribution, influenced by climate and trophic conditions at the source sites as well as by the age of culture and growth phase. Accordingly, when considering FAs for the determination of lower taxonomic ranks we recommend using the term "trait" instead of "biomarker", as the latter designates unchangeable "fingerprint" of a specific taxon. Furthermore, despite that desmids were regarded as microalgae having stable genomes, long-term cultivation appeared to cause modifications in FA metabolic pathways, evident as a larger proportion of stearidonic acid in desmid strains cultivated over extensive time periods (>35 years).
T2  - Phytochemistry
T1  - Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.
VL  - 170
DO  - 10.1016/j.phytochem.2019.112200
SP  - 112200
ER  - 

@article{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders K. and Wulff, Angela",
year = "2020",
abstract = "Desmids (Zygnematophyceae) are a group of poorly studied green microalgae. The aim of the present study was to identify fatty acids (FAs) that could be used as biomarkers in desmids in general, and to determine FAs as traits within different ecophysiological desmid groups. FA profiles of 29 desmid strains were determined and analysed with respect to their geographic origin, trophic preference and age of cultivation. It appeared that merely FAs present in relatively large proportions such as palmitic, linoleic, α-linolenic and hexadecatrienoic acids could be used as biomarkers for reliable categorization of this microalgal group. Linear discriminant analysis applied to three a priori defined groups of desmids, revealed clear strain-specific characteristics regarding FA distribution, influenced by climate and trophic conditions at the source sites as well as by the age of culture and growth phase. Accordingly, when considering FAs for the determination of lower taxonomic ranks we recommend using the term "trait" instead of "biomarker", as the latter designates unchangeable "fingerprint" of a specific taxon. Furthermore, despite that desmids were regarded as microalgae having stable genomes, long-term cultivation appeared to cause modifications in FA metabolic pathways, evident as a larger proportion of stearidonic acid in desmid strains cultivated over extensive time periods (>35 years).",
journal = "Phytochemistry",
title = "Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.",
volume = "170",
doi = "10.1016/j.phytochem.2019.112200",
pages = "112200"
}

Stamenković, M., Steinwall, E., Nilsson, A. K.,& Wulff, A.. (2020). Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.. in Phytochemistry, 170, 112200.
https://doi.org/10.1016/j.phytochem.2019.112200

Stamenković M, Steinwall E, Nilsson AK, Wulff A. Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.. in Phytochemistry. 2020;170:112200.
doi:10.1016/j.phytochem.2019.112200 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders K., Wulff, Angela, "Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach." in Phytochemistry, 170 (2020):112200,
https://doi.org/10.1016/j.phytochem.2019.112200 . .

TY  - JOUR
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders K.
AU  - Wulff, Angela
PY  - 2018
UR  - http://link.springer.com/10.1007/s10811-018-1598-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3129
AB  - This study aimed to investigate fatty acid content and productivity of the insufficiently investigated group of freshwater microalgae—desmids (Zygnematophyceae, Streptophyta)—and to estimate their commercial potential. A total of 29 desmid strains of various environmental preferences were grown in standard cultivation conditions to assess fatty acid concentration and composition and biomass productivity during the growth phases. Six desmid strains belonging to Cosmarium crenatum var. boldtianum, C. meneghinii, C. regnellii var. pseudoregnellii, C. leave, Staurastrum boreale, and S. punctulatum had distinctly high total fatty acid contents (> 200 mg g−1 dry weight), among which C. crenatum had by far the highest average of total fatty acids (308.1 mg g−1 dry weight). Despite that desmids were grown in a medium which was not enriched with nutrients and CO2, these six strains achieved moderate biomass productivity (up to 0.14 g dry weight L−1 day−1), while the fatty acid productivity was in the range 8–11.1 mg L−1 day−1. The relatively high amounts of linoleic and palmitic acids in C. crenatum var. boldtianum and C. meneghinii were comparable to those found in several commercially grown plants, indicating that fatty acid extracts of these desmids could be utilized in cosmetics, pharmacy, medicine, or in additional industrial applications. On the other hand, the high proportion of oleic acid in a new isolate of Staurastrum boreale, along with its relatively high biomass productivity and cell size, pointed that this strain might be used for further investigations regarding biodiesel production.
T2  - Journal of Applied Phycology
T1  - Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study
DO  - 10.1007/s10811-018-1598-8
ER  - 

@article{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders K. and Wulff, Angela",
year = "2018",
abstract = "This study aimed to investigate fatty acid content and productivity of the insufficiently investigated group of freshwater microalgae—desmids (Zygnematophyceae, Streptophyta)—and to estimate their commercial potential. A total of 29 desmid strains of various environmental preferences were grown in standard cultivation conditions to assess fatty acid concentration and composition and biomass productivity during the growth phases. Six desmid strains belonging to Cosmarium crenatum var. boldtianum, C. meneghinii, C. regnellii var. pseudoregnellii, C. leave, Staurastrum boreale, and S. punctulatum had distinctly high total fatty acid contents (> 200 mg g−1 dry weight), among which C. crenatum had by far the highest average of total fatty acids (308.1 mg g−1 dry weight). Despite that desmids were grown in a medium which was not enriched with nutrients and CO2, these six strains achieved moderate biomass productivity (up to 0.14 g dry weight L−1 day−1), while the fatty acid productivity was in the range 8–11.1 mg L−1 day−1. The relatively high amounts of linoleic and palmitic acids in C. crenatum var. boldtianum and C. meneghinii were comparable to those found in several commercially grown plants, indicating that fatty acid extracts of these desmids could be utilized in cosmetics, pharmacy, medicine, or in additional industrial applications. On the other hand, the high proportion of oleic acid in a new isolate of Staurastrum boreale, along with its relatively high biomass productivity and cell size, pointed that this strain might be used for further investigations regarding biodiesel production.",
journal = "Journal of Applied Phycology",
title = "Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study",
doi = "10.1007/s10811-018-1598-8"
}

Stamenković, M., Steinwall, E., Nilsson, A. K.,& Wulff, A.. (2018). Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study. in Journal of Applied Phycology.
https://doi.org/10.1007/s10811-018-1598-8

Stamenković M, Steinwall E, Nilsson AK, Wulff A. Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study. in Journal of Applied Phycology. 2018;.
doi:10.1007/s10811-018-1598-8 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders K., Wulff, Angela, "Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study" in Journal of Applied Phycology (2018),
https://doi.org/10.1007/s10811-018-1598-8 . .