TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32077821
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3757
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science Publishers Ltd.
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS.
IS  - 8
VL  - 21
DO  - 10.2174/1389450121666200220123305
SP  - 776
EP  - 780
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS.",
number = "8",
volume = "21",
doi = "10.2174/1389450121666200220123305",
pages = "776-780"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS.. in Current Drug Targets
Bentham Science Publishers Ltd.., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS.. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS." in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - JOUR
AU  - Brkušanin, Milos
AU  - Jeftović-Velkova, Irena
AU  - Jovanović, Vladimir
AU  - Perić, Stojan
AU  - Pešović, Jovan
AU  - Brajušković, Goran
AU  - Stević, Zorica
AU  - Savić-Pavićević, Dušanka
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0370-81791800069B
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3452
AB  - Introduction/Objective. Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron disease. The majority of cases are apparently sporadic ALS (SALS) with variants in susceptibility genes or sometimes in high-risk ALS genes. Two ALS susceptibility genes are SMN1, whose functional loss causes spinal muscular atrophy (SMA), and a nearly identical SMN2 gene, which modulates SMA severity. In this study we examined the association of copy number variations (CNVs) of SMN1 and SMN2 genes and two additional genes, SERF1 and NAIP, residing in the same genomic region (i.e. 5q13.2 segmental duplication), with SALS in patients from Serbia. Methods. Multiplex ligation-dependent probe amplification was used to determine CNVs of each gene in a clinically well-characterised group of 153 Serbian SALS patients and 153 controls. Results. Individual association between SMN1, SMN2, SERF1 or NAIP CNVs and SALS susceptibility or survival was not found. Survival curves based on the multivariable Cox regression analysis showed that three SMN1 copies, lower ALS Functional Rating Scale Revised (ALSFRS-R) score at the time of diagnosis, faster decline of the ALSFRS-R score over time, and shorter diagnostic delay result in shorter survival of Serbian SALS patients. Conclusion. Clinical variables might be complemented with the SMN1 copy number to improve prediction of survival in Serbian SALS patients.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis
IS  - 11-12
VL  - 146
DO  - 10.2298/SARH180801069B
SP  - 646
EP  - 652
ER  - 

@article{
author = "Brkušanin, Milos and Jeftović-Velkova, Irena and Jovanović, Vladimir and Perić, Stojan and Pešović, Jovan and Brajušković, Goran and Stević, Zorica and Savić-Pavićević, Dušanka",
year = "2018",
abstract = "Introduction/Objective. Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron disease. The majority of cases are apparently sporadic ALS (SALS) with variants in susceptibility genes or sometimes in high-risk ALS genes. Two ALS susceptibility genes are SMN1, whose functional loss causes spinal muscular atrophy (SMA), and a nearly identical SMN2 gene, which modulates SMA severity. In this study we examined the association of copy number variations (CNVs) of SMN1 and SMN2 genes and two additional genes, SERF1 and NAIP, residing in the same genomic region (i.e. 5q13.2 segmental duplication), with SALS in patients from Serbia. Methods. Multiplex ligation-dependent probe amplification was used to determine CNVs of each gene in a clinically well-characterised group of 153 Serbian SALS patients and 153 controls. Results. Individual association between SMN1, SMN2, SERF1 or NAIP CNVs and SALS susceptibility or survival was not found. Survival curves based on the multivariable Cox regression analysis showed that three SMN1 copies, lower ALS Functional Rating Scale Revised (ALSFRS-R) score at the time of diagnosis, faster decline of the ALSFRS-R score over time, and shorter diagnostic delay result in shorter survival of Serbian SALS patients. Conclusion. Clinical variables might be complemented with the SMN1 copy number to improve prediction of survival in Serbian SALS patients.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis",
number = "11-12",
volume = "146",
doi = "10.2298/SARH180801069B",
pages = "646-652"
}

Brkušanin, M., Jeftović-Velkova, I., Jovanović, V., Perić, S., Pešović, J., Brajušković, G., Stević, Z.,& Savić-Pavićević, D.. (2018). SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis. in Srpski arhiv za celokupno lekarstvo, 146(11-12), 646-652.
https://doi.org/10.2298/SARH180801069B

Brkušanin M, Jeftović-Velkova I, Jovanović V, Perić S, Pešović J, Brajušković G, Stević Z, Savić-Pavićević D. SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis. in Srpski arhiv za celokupno lekarstvo. 2018;146(11-12):646-652.
doi:10.2298/SARH180801069B .

Brkušanin, Milos, Jeftović-Velkova, Irena, Jovanović, Vladimir, Perić, Stojan, Pešović, Jovan, Brajušković, Goran, Stević, Zorica, Savić-Pavićević, Dušanka, "SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis" in Srpski arhiv za celokupno lekarstvo, 146, no. 11-12 (2018):646-652,
https://doi.org/10.2298/SARH180801069B . .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica
AU  - Baščarević, Vladimir
AU  - Živić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://www.liebertpub.com/doi/10.1089/ars.2017.7433
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3065
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 28, 1570-1575.
T2  - Antioxidants and Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
IS  - 17
VL  - 28
DO  - 10.1089/ars.2017.7433
SP  - 1570
EP  - 1575
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica and Baščarević, Vladimir and Živić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 28, 1570-1575.",
journal = "Antioxidants and Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433",
pages = "1570-1575"
}

Opačić, M., Stević, Z., Baščarević, V., Živić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants and Redox Signaling, 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević Z, Baščarević V, Živić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants and Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica, Baščarević, Vladimir, Živić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants and Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - CONF
AU  - Milićević, Katarina
AU  - Milošević, Milena
AU  - Božić, Iva
AU  - Lavrnja, Irena
AU  - Stevanović, Ivana
AU  - Bijelić, Dunja D.
AU  - Živković, Irena
AU  - Stević, Zorica
AU  - Anđus, Pavle R.
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5990
AB  - Introduction. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder that affects motor neurons. Having in mind well documented facts that on one hand, ALS brain is under oxidative stress, and on the other that non-cell autonomous mechanisms involving glial cells contribute to the disease progression, we wanted to examine the effect of humoral factors immunoglobulins G from ALS patients (ALS IgG) on oxidative stress and antioxidative system of BV-2 microglial cell line. Methods. BV-2 cells were treated with ALS and control IgG (0.1 mg/ml). TNF-α release, oxidative stress markers and antioxidative enzymes activities were determined using biochemical assays (24 h treatment), while gene expression was determined using RT-qPCR (4 h treatment). ROS, cytosolic peroxide and pH alteration were evaluated with carboxy-H2DCFDA, HyPer and SypHer, respectively. Results. All tested ALS IgG (compared with control IgG) induced oxidative stress (rise in NO and lipid peroxidation), release of TNF-α and higher antioxidative defense (elevation of Mn- and Cu,Zn-superoxide dismutase, catalase, glutathione reductase with a decrease of glutathione peroxidase and glutathione). IgG from 4/11 ALS patients induced slow exponential rise of HyPer intensity and lower increase of SypHer intensity. None of the control IgG induced changes with neither of the indicators. Acute ROS generation was detected in 1/3 of ALS samples with carboxy-H2DCFDA. Conclusion. Our study demonstrates the potential role of inflammatory humoral factors, ALS IgGs, as triggers (via ROS generation) of the activation in microglia, known to occur in later stages of the disease.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
T1  - Serum IGG fraction from ALS patients alters redox homeostasis in the BV-2 microglial cell line
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5990
ER  - 

@conference{
author = "Milićević, Katarina and Milošević, Milena and Božić, Iva and Lavrnja, Irena and Stevanović, Ivana and Bijelić, Dunja D. and Živković, Irena and Stević, Zorica and Anđus, Pavle R.",
year = "2017",
abstract = "Introduction. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder that affects motor neurons. Having in mind well documented facts that on one hand, ALS brain is under oxidative stress, and on the other that non-cell autonomous mechanisms involving glial cells contribute to the disease progression, we wanted to examine the effect of humoral factors immunoglobulins G from ALS patients (ALS IgG) on oxidative stress and antioxidative system of BV-2 microglial cell line. Methods. BV-2 cells were treated with ALS and control IgG (0.1 mg/ml). TNF-α release, oxidative stress markers and antioxidative enzymes activities were determined using biochemical assays (24 h treatment), while gene expression was determined using RT-qPCR (4 h treatment). ROS, cytosolic peroxide and pH alteration were evaluated with carboxy-H2DCFDA, HyPer and SypHer, respectively. Results. All tested ALS IgG (compared with control IgG) induced oxidative stress (rise in NO and lipid peroxidation), release of TNF-α and higher antioxidative defense (elevation of Mn- and Cu,Zn-superoxide dismutase, catalase, glutathione reductase with a decrease of glutathione peroxidase and glutathione). IgG from 4/11 ALS patients induced slow exponential rise of HyPer intensity and lower increase of SypHer intensity. None of the control IgG induced changes with neither of the indicators. Acute ROS generation was detected in 1/3 of ALS samples with carboxy-H2DCFDA. Conclusion. Our study demonstrates the potential role of inflammatory humoral factors, ALS IgGs, as triggers (via ROS generation) of the activation in microglia, known to occur in later stages of the disease.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia",
title = "Serum IGG fraction from ALS patients alters redox homeostasis in the BV-2 microglial cell line",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5990"
}

Milićević, K., Milošević, M., Božić, I., Lavrnja, I., Stevanović, I., Bijelić, D. D., Živković, I., Stević, Z.,& Anđus, P. R.. (2017). Serum IGG fraction from ALS patients alters redox homeostasis in the BV-2 microglial cell line. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 72.
https://hdl.handle.net/21.15107/rcub_ibiss_5990

Milićević K, Milošević M, Božić I, Lavrnja I, Stevanović I, Bijelić DD, Živković I, Stević Z, Anđus PR. Serum IGG fraction from ALS patients alters redox homeostasis in the BV-2 microglial cell line. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia. 2017;:72.
https://hdl.handle.net/21.15107/rcub_ibiss_5990 .

Milićević, Katarina, Milošević, Milena, Božić, Iva, Lavrnja, Irena, Stevanović, Ivana, Bijelić, Dunja D., Živković, Irena, Stević, Zorica, Anđus, Pavle R., "Serum IGG fraction from ALS patients alters redox homeostasis in the BV-2 microglial cell line" in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia (2017):72,
https://hdl.handle.net/21.15107/rcub_ibiss_5990 .

TY  - JOUR
AU  - Milošević, Milena
AU  - Milićević, Katarina
AU  - Božić, Iva
AU  - Lavrnja, Irena
AU  - Stevanović, Ivana
AU  - Bijelić, Dunja
AU  - Dubaić, Marija
AU  - Živković, Irena
AU  - Stević, Zorica
AU  - Giniatullin, Rashid
AU  - Andjus, Pavle
PY  - 2017
UR  - http://journal.frontiersin.org/article/10.3389/fimmu.2017.01619/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2928
AB  - Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder with a very fast progression, no diagnostic tool for the presymptomatic phase, and still no effective treatment of the disease. Although ALS affects motor neurons, the overall pathophysiological condition points out to the non-cell autonomous mechanisms, where astrocytes and microglia play crucial roles in the disease progression. We have already shown that IgG from sera of ALS patients (ALS IgG) induce calcium transients and an increase in the mobility of acidic vesicles in cultured rat astrocytes. Having in mind the role of microglia in neurodegeneration, and a well-documented fact that oxidative stress is one of the many components contributing to the disease, we decided to examine the effect of ALS IgG on activation, oxidative stress and antioxidative system of BV-2 microglia, and to evaluate their acute effect on cytosolic peroxide, pH, and on reactive oxygen species (ROS) generation. All tested ALS IgGs (compared to control IgG) induced oxidative stress (rise in nitric oxide and the index of lipid peroxidation) followed by release of TNF-α and higher antioxidative defense (elevation of Mn- and CuZn-superoxide dismutase, catalase, and glutathione reductase with a decrease of glutathione peroxidase and glutathione) after 24 h treatment. Both ALS IgG and control IgG showed same localization on the membrane of BV-2 cells following 24 h treatment. Cytosolic peroxide and pH alteration were evaluated with fluorescent probes HyPer and SypHer, respectively, having in mind that HyPer also reacts to pH changes. Out of 11 tested IgGs from ALS patients, 4 induced slow exponential rise of HyPer signal, with maximal normalized fluorescence in the range 0.2–0.5, also inducing similar increase of SypHer intensity, but of a lower amplitude. None of the control IgGs induced changes with neither of the indicators. Acute ROS generation was detected in one out of three tested ALS samples with carboxy-H2DCFDA. The observed phenomena demonstrate the potential role of inflammatory humoral factors, IgGs, as potential triggers of the activation in microglia, known to occur in later stages of ALS. Therefore, revealing the ALS IgG signaling cascade in microglial cells could offer a valuable molecular biomarker and/or a potential therapeutic target.
T2  - Frontiers in Immunology
T1  - Immunoglobulins G from Sera of Amyotrophic Lateral Sclerosis Patients Induce Oxidative Stress and Upregulation of Antioxidative System in BV-2 Microglial Cell Line
IS  - NOV
VL  - 8
DO  - 10.3389/fimmu.2017.01619
SP  - 1619
ER  - 

@article{
author = "Milošević, Milena and Milićević, Katarina and Božić, Iva and Lavrnja, Irena and Stevanović, Ivana and Bijelić, Dunja and Dubaić, Marija and Živković, Irena and Stević, Zorica and Giniatullin, Rashid and Andjus, Pavle",
year = "2017",
abstract = "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder with a very fast progression, no diagnostic tool for the presymptomatic phase, and still no effective treatment of the disease. Although ALS affects motor neurons, the overall pathophysiological condition points out to the non-cell autonomous mechanisms, where astrocytes and microglia play crucial roles in the disease progression. We have already shown that IgG from sera of ALS patients (ALS IgG) induce calcium transients and an increase in the mobility of acidic vesicles in cultured rat astrocytes. Having in mind the role of microglia in neurodegeneration, and a well-documented fact that oxidative stress is one of the many components contributing to the disease, we decided to examine the effect of ALS IgG on activation, oxidative stress and antioxidative system of BV-2 microglia, and to evaluate their acute effect on cytosolic peroxide, pH, and on reactive oxygen species (ROS) generation. All tested ALS IgGs (compared to control IgG) induced oxidative stress (rise in nitric oxide and the index of lipid peroxidation) followed by release of TNF-α and higher antioxidative defense (elevation of Mn- and CuZn-superoxide dismutase, catalase, and glutathione reductase with a decrease of glutathione peroxidase and glutathione) after 24 h treatment. Both ALS IgG and control IgG showed same localization on the membrane of BV-2 cells following 24 h treatment. Cytosolic peroxide and pH alteration were evaluated with fluorescent probes HyPer and SypHer, respectively, having in mind that HyPer also reacts to pH changes. Out of 11 tested IgGs from ALS patients, 4 induced slow exponential rise of HyPer signal, with maximal normalized fluorescence in the range 0.2–0.5, also inducing similar increase of SypHer intensity, but of a lower amplitude. None of the control IgGs induced changes with neither of the indicators. Acute ROS generation was detected in one out of three tested ALS samples with carboxy-H2DCFDA. The observed phenomena demonstrate the potential role of inflammatory humoral factors, IgGs, as potential triggers of the activation in microglia, known to occur in later stages of ALS. Therefore, revealing the ALS IgG signaling cascade in microglial cells could offer a valuable molecular biomarker and/or a potential therapeutic target.",
journal = "Frontiers in Immunology",
title = "Immunoglobulins G from Sera of Amyotrophic Lateral Sclerosis Patients Induce Oxidative Stress and Upregulation of Antioxidative System in BV-2 Microglial Cell Line",
number = "NOV",
volume = "8",
doi = "10.3389/fimmu.2017.01619",
pages = "1619"
}

Milošević, M., Milićević, K., Božić, I., Lavrnja, I., Stevanović, I., Bijelić, D., Dubaić, M., Živković, I., Stević, Z., Giniatullin, R.,& Andjus, P.. (2017). Immunoglobulins G from Sera of Amyotrophic Lateral Sclerosis Patients Induce Oxidative Stress and Upregulation of Antioxidative System in BV-2 Microglial Cell Line. in Frontiers in Immunology, 8(NOV), 1619.
https://doi.org/10.3389/fimmu.2017.01619

Milošević M, Milićević K, Božić I, Lavrnja I, Stevanović I, Bijelić D, Dubaić M, Živković I, Stević Z, Giniatullin R, Andjus P. Immunoglobulins G from Sera of Amyotrophic Lateral Sclerosis Patients Induce Oxidative Stress and Upregulation of Antioxidative System in BV-2 Microglial Cell Line. in Frontiers in Immunology. 2017;8(NOV):1619.
doi:10.3389/fimmu.2017.01619 .

Milošević, Milena, Milićević, Katarina, Božić, Iva, Lavrnja, Irena, Stevanović, Ivana, Bijelić, Dunja, Dubaić, Marija, Živković, Irena, Stević, Zorica, Giniatullin, Rashid, Andjus, Pavle, "Immunoglobulins G from Sera of Amyotrophic Lateral Sclerosis Patients Induce Oxidative Stress and Upregulation of Antioxidative System in BV-2 Microglial Cell Line" in Frontiers in Immunology, 8, no. NOV (2017):1619,
https://doi.org/10.3389/fimmu.2017.01619 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Marinković, Dragan
AU  - Perić, Stojan
AU  - Stević, Zorica
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Rakočević Stojanović, Vidosava
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6358
AB  - Objectives: The aim of our study was to determine if redox imbalance caused by the activities of antioxidant
enzymes existed in erythrocytes of type 1 myotonic dystrophy (DM1) patients.
Methods: The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase, and
glutathione reductase were measured in 30 DM1 patients and 15 healthy controls (HCs). The obtained
values were correlated with the Muscular Impairment Rating Scale (MIRS) score and creatine kinase (CK).
Results: Superoxide dismutase and catalase activities were lower in DM1 patients compared to HCs. A
positive correlation was found between disease duration and MIRS score as well as with glutathione
reductase activity. In DM1 patients, there were positive correlations between catalase, glutathione
peroxidase, and glutathione reductase activities. After sub-dividing DM1 patients according to CK levels,
superoxide dismutase activity was still statistically different from HCs. However, catalase activity was
significantly lower only in DM1 patients with increased CK.
Discussion: Undesirable alterations in antioxidant enzyme activities during DM1 disease progression may
result in conditions favoring oxidative stress and changes in metabolism which together could contribute
to muscle wasting.
PB  - Abingdon: Taylor and Francis
T2  - Redox Report
T1  - Redox imbalance in peripheral blood of type 1 myotonic dystrophy patients
IS  - 5
VL  - 21
DO  - 10.1080/13510002.2015.1107311
SP  - 232
EP  - 237
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Marinković, Dragan and Perić, Stojan and Stević, Zorica and Spasić, Mihajlo and Blagojević, Duško and Rakočević Stojanović, Vidosava",
year = "2016",
abstract = "Objectives: The aim of our study was to determine if redox imbalance caused by the activities of antioxidant
enzymes existed in erythrocytes of type 1 myotonic dystrophy (DM1) patients.
Methods: The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase, and
glutathione reductase were measured in 30 DM1 patients and 15 healthy controls (HCs). The obtained
values were correlated with the Muscular Impairment Rating Scale (MIRS) score and creatine kinase (CK).
Results: Superoxide dismutase and catalase activities were lower in DM1 patients compared to HCs. A
positive correlation was found between disease duration and MIRS score as well as with glutathione
reductase activity. In DM1 patients, there were positive correlations between catalase, glutathione
peroxidase, and glutathione reductase activities. After sub-dividing DM1 patients according to CK levels,
superoxide dismutase activity was still statistically different from HCs. However, catalase activity was
significantly lower only in DM1 patients with increased CK.
Discussion: Undesirable alterations in antioxidant enzyme activities during DM1 disease progression may
result in conditions favoring oxidative stress and changes in metabolism which together could contribute
to muscle wasting.",
publisher = "Abingdon: Taylor and Francis",
journal = "Redox Report",
title = "Redox imbalance in peripheral blood of type 1 myotonic dystrophy patients",
number = "5",
volume = "21",
doi = "10.1080/13510002.2015.1107311",
pages = "232-237"
}

Nikolić-Kokić, A., Marinković, D., Perić, S., Stević, Z., Spasić, M., Blagojević, D.,& Rakočević Stojanović, V.. (2016). Redox imbalance in peripheral blood of type 1 myotonic dystrophy patients. in Redox Report
Abingdon: Taylor and Francis., 21(5), 232-237.
https://doi.org/10.1080/13510002.2015.1107311

Nikolić-Kokić A, Marinković D, Perić S, Stević Z, Spasić M, Blagojević D, Rakočević Stojanović V. Redox imbalance in peripheral blood of type 1 myotonic dystrophy patients. in Redox Report. 2016;21(5):232-237.
doi:10.1080/13510002.2015.1107311 .

Nikolić-Kokić, Aleksandra, Marinković, Dragan, Perić, Stojan, Stević, Zorica, Spasić, Mihajlo, Blagojević, Duško, Rakočević Stojanović, Vidosava, "Redox imbalance in peripheral blood of type 1 myotonic dystrophy patients" in Redox Report, 21, no. 5 (2016):232-237,
https://doi.org/10.1080/13510002.2015.1107311 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica
AU  - Anđus, Pavle
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6080
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction
IS  - 1
VL  - 67
DO  - 10.2298/ABS141006023N
SP  - 187
EP  - 192
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica and Anđus, Pavle and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N",
pages = "187-192"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z., Anđus, P.,& Spasić, M.. (2015). The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević Z, Anđus P, Spasić M. The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica, Anđus, Pavle, Spasić, Mihajlo, "The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Spasojević, Ivan
AU  - Stević, Zorica
AU  - Spasić, Mihajlo
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/504
AB  - Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
AB  - Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.
T2  - Journal of Medical Biochemistry
T1  - Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
T1  - The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
IS  - 4
VL  - 32
SP  - 375
EP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_504
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Blagojević, Duško and Oreščanin Dušić, Zorana and Slavić, Marija and Spasojević, Ivan and Stević, Zorica and Spasić, Mihajlo",
year = "2013, 2013",
abstract = "Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS., Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.",
journal = "Journal of Medical Biochemistry",
title = "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića, The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions",
number = "4",
volume = "32",
pages = "375-379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_504"
}

Nikolić-Kokić, A., Blagojević, D., Oreščanin Dušić, Z., Slavić, M., Spasojević, I., Stević, Z.,& Spasić, M.. (2013). Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry, 32(4), 375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504

Nikolić-Kokić A, Blagojević D, Oreščanin Dušić Z, Slavić M, Spasojević I, Stević Z, Spasić M. Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry. 2013;32(4):375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

Nikolić-Kokić, Aleksandra, Blagojević, Duško, Oreščanin Dušić, Zorana, Slavić, Marija, Spasojević, Ivan, Stević, Zorica, Spasić, Mihajlo, "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića" in Journal of Medical Biochemistry, 32, no. 4 (2013):375-379,
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

TY  - JOUR
AU  - Nikolić, Aleksandra L.
AU  - Stević, Zorica
AU  - Blagojević, Duško
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/38
AB  - Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls.
AB  - U ovom radu ispitivana je aktivnost: bakar cink sadržavajuće superoksid dismutaze (Cu, Zn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation-S-transferaze (GST) u krvi pacijenata sa familijarnim oblikom amiotrofične lateralne skleroze (FALS) sa mutacijom Leu144Phe, asimptomskim nosiocima mutacije Leu144Phe i kontrola. Aktivnost Cu,Zn SOD je statistički značajno niža kod FALS pacijenata i asimptomskih nosioca mutacije Leu144Phe nego kod kontrola (p < 0,001). Kod FALS pacijenata aktivnost GSH-Px je niža (p < 0,01), a aktivnost GR je veća (p < 0,001) u poređenju sa kontrolnom grupom. Kanonijska diskriminantna analiza obezbeđuje statističku podršku uočene razlike u sastavu antioksidacionih zaštitnih enzima u krvi ispitivanih grupa i pokazuje nam da svaka komponenta značajno doprinosi toj razlici. Naši rezultati sugerišu da je oksidacioni stres uključen u patogenezu FALS i da su antioksidacioni zaštitni enzimi izloženi različitom oksidacionom pritisku kod FALS, asimptomskih nosioca mutacije Leu144Phe i kontrola.
T2  - Jugoslovenska medicinska biohemija
T1  - Aktivnost antioksidacionih zaštitnih enzima u krvi ljudi sa Leu144Phe mutacijom
T1  - Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation
IS  - 2
VL  - 24
SP  - 111
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_38
ER  - 

@article{
author = "Nikolić, Aleksandra L. and Stević, Zorica and Blagojević, Duško and Saičić, Zorica and Spasić, Mihajlo",
year = "2005, 2005",
abstract = "Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls., U ovom radu ispitivana je aktivnost: bakar cink sadržavajuće superoksid dismutaze (Cu, Zn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation-S-transferaze (GST) u krvi pacijenata sa familijarnim oblikom amiotrofične lateralne skleroze (FALS) sa mutacijom Leu144Phe, asimptomskim nosiocima mutacije Leu144Phe i kontrola. Aktivnost Cu,Zn SOD je statistički značajno niža kod FALS pacijenata i asimptomskih nosioca mutacije Leu144Phe nego kod kontrola (p < 0,001). Kod FALS pacijenata aktivnost GSH-Px je niža (p < 0,01), a aktivnost GR je veća (p < 0,001) u poređenju sa kontrolnom grupom. Kanonijska diskriminantna analiza obezbeđuje statističku podršku uočene razlike u sastavu antioksidacionih zaštitnih enzima u krvi ispitivanih grupa i pokazuje nam da svaka komponenta značajno doprinosi toj razlici. Naši rezultati sugerišu da je oksidacioni stres uključen u patogenezu FALS i da su antioksidacioni zaštitni enzimi izloženi različitom oksidacionom pritisku kod FALS, asimptomskih nosioca mutacije Leu144Phe i kontrola.",
journal = "Jugoslovenska medicinska biohemija",
title = "Aktivnost antioksidacionih zaštitnih enzima u krvi ljudi sa Leu144Phe mutacijom, Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation",
number = "2",
volume = "24",
pages = "111-114",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_38"
}

Nikolić, A. L., Stević, Z., Blagojević, D., Saičić, Z.,& Spasić, M.. (2005). Aktivnost antioksidacionih zaštitnih enzima u krvi ljudi sa Leu144Phe mutacijom. in Jugoslovenska medicinska biohemija, 24(2), 111-114.
https://hdl.handle.net/21.15107/rcub_ibiss_38

Nikolić AL, Stević Z, Blagojević D, Saičić Z, Spasić M. Aktivnost antioksidacionih zaštitnih enzima u krvi ljudi sa Leu144Phe mutacijom. in Jugoslovenska medicinska biohemija. 2005;24(2):111-114.
https://hdl.handle.net/21.15107/rcub_ibiss_38 .

Nikolić, Aleksandra L., Stević, Zorica, Blagojević, Duško, Saičić, Zorica, Spasić, Mihajlo, "Aktivnost antioksidacionih zaštitnih enzima u krvi ljudi sa Leu144Phe mutacijom" in Jugoslovenska medicinska biohemija, 24, no. 2 (2005):111-114,
https://hdl.handle.net/21.15107/rcub_ibiss_38 .