TY  - JOUR
AU  - Jakovljević, Danijel
AU  - Nikolić, Milan
AU  - Jovanović, Vesna
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić-Kokić, Aleksandra
AU  - Novaković, Emilija
AU  - Miljević, Čedo
AU  - Milovanovć, Maja
AU  - Blagojević, Duško
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6510
AB  - Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood
IS  - 1
VL  - 17
DO  - 10.3390/ph17010130
SP  - 130
ER  - 

@article{
author = "Jakovljević, Danijel and Nikolić, Milan and Jovanović, Vesna and Vidonja Uzelac, Teodora and Nikolić-Kokić, Aleksandra and Novaković, Emilija and Miljević, Čedo and Milovanovć, Maja and Blagojević, Duško",
year = "2024",
abstract = "Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood",
number = "1",
volume = "17",
doi = "10.3390/ph17010130",
pages = "130"
}

Jakovljević, D., Nikolić, M., Jovanović, V., Vidonja Uzelac, T., Nikolić-Kokić, A., Novaković, E., Miljević, Č., Milovanovć, M.,& Blagojević, D.. (2024). Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals
Basel: MDPI., 17(1), 130.
https://doi.org/10.3390/ph17010130

Jakovljević D, Nikolić M, Jovanović V, Vidonja Uzelac T, Nikolić-Kokić A, Novaković E, Miljević Č, Milovanovć M, Blagojević D. Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals. 2024;17(1):130.
doi:10.3390/ph17010130 .

Jakovljević, Danijel, Nikolić, Milan, Jovanović, Vesna, Vidonja Uzelac, Teodora, Nikolić-Kokić, Aleksandra, Novaković, Emilija, Miljević, Čedo, Milovanovć, Maja, Blagojević, Duško, "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood" in Pharmaceuticals, 17, no. 1 (2024):130,
https://doi.org/10.3390/ph17010130 . .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana D.
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan R.
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0009279719310257?via%3Dihub
UR  - http://www.ncbi.nlm.nih.gov/pubmed/31400341
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3454
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.
VL  - 311
DO  - 10.1016/j.cbi.2019.108787
SP  - 108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana D. and Mačvanin, Mirjana T. and Nikolić, Milan R. and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.",
volume = "311",
doi = "10.1016/j.cbi.2019.108787",
pages = "108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S. D., Mačvanin, M. T., Nikolić, M. R., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.. in Chemico-Biological Interactions, 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić SD, Mačvanin MT, Nikolić MR, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content.. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana D., Mačvanin, Mirjana T., Nikolić, Milan R., Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content." in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .