TY  - JOUR
AU  - Radulović, Nataša
AU  - Pilipović, Ivan
AU  - Stojanović, Ivana D.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6472
AB  - Cyclophosphamide (CP) is a cytostatic, widely used to treat different carcinomas and autoimmune diseases. It is
commonly used in experimental designs modeling immunosuppression in laboratory animals, with different approaches for
CP treatment but without a consensus on the dose, timing, and route of administration. We aimed to establish if treatment
with CP in C57BL/6 mice depletes regulatory T cells (Tregs). Tregs are a crucial component of the immune system that
helps maintain immune tolerance and prevent excessive immune reactions. They are significant in autoimmune diseases,
allergies, and immune-related therapies. CP was applied intraperitoneally (i.p.) twice in a 5-day interval in doses of 100 mg/
kg. Monitoring of Treg prevalence in peripheral blood after each treatment and in the spleen after the second treatment with
CP revealed a drop in the number of Tregs after two doses of CP because of the decreased number of total lymphocytes but
not as a specific response of the Tregs. The prevalence of Tregs in peripheral blood after CP treatment mirrored the change
in Treg number in the spleen. CP treatment induced a decrease in the number of CD3+ cells in the spleen while increasing
their proportion, indicating that CP affected the B lymphocyte population rather than T cells. Our results suggest that CP
treatment cannot be used as a specific Treg-depleting agent in the C57BL/6 animal model.
PB  - Belgrade: Serbian Biological Society
T2  - Archive of Biological Sciences
T1  - Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model
IS  - 4
VL  - 75
DO  - 10.2298/ABS230715032R
SP  - 397
EP  - 406
ER  - 

@article{
author = "Radulović, Nataša and Pilipović, Ivan and Stojanović, Ivana D.",
year = "2023",
abstract = "Cyclophosphamide (CP) is a cytostatic, widely used to treat different carcinomas and autoimmune diseases. It is
commonly used in experimental designs modeling immunosuppression in laboratory animals, with different approaches for
CP treatment but without a consensus on the dose, timing, and route of administration. We aimed to establish if treatment
with CP in C57BL/6 mice depletes regulatory T cells (Tregs). Tregs are a crucial component of the immune system that
helps maintain immune tolerance and prevent excessive immune reactions. They are significant in autoimmune diseases,
allergies, and immune-related therapies. CP was applied intraperitoneally (i.p.) twice in a 5-day interval in doses of 100 mg/
kg. Monitoring of Treg prevalence in peripheral blood after each treatment and in the spleen after the second treatment with
CP revealed a drop in the number of Tregs after two doses of CP because of the decreased number of total lymphocytes but
not as a specific response of the Tregs. The prevalence of Tregs in peripheral blood after CP treatment mirrored the change
in Treg number in the spleen. CP treatment induced a decrease in the number of CD3+ cells in the spleen while increasing
their proportion, indicating that CP affected the B lymphocyte population rather than T cells. Our results suggest that CP
treatment cannot be used as a specific Treg-depleting agent in the C57BL/6 animal model.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archive of Biological Sciences",
title = "Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model",
number = "4",
volume = "75",
doi = "10.2298/ABS230715032R",
pages = "397-406"
}

Radulović, N., Pilipović, I.,& Stojanović, I. D.. (2023). Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model. in Archive of Biological Sciences
Belgrade: Serbian Biological Society., 75(4), 397-406.
https://doi.org/10.2298/ABS230715032R

Radulović N, Pilipović I, Stojanović ID. Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model. in Archive of Biological Sciences. 2023;75(4):397-406.
doi:10.2298/ABS230715032R .

Radulović, Nataša, Pilipović, Ivan, Stojanović, Ivana D., "Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model" in Archive of Biological Sciences, 75, no. 4 (2023):397-406,
https://doi.org/10.2298/ABS230715032R . .

TY  - JOUR
AU  - Fuchs, Anke
AU  - Gliwiński, Mateusz
AU  - Grageda, Nathali
AU  - Spiering, Rachel
AU  - Abbas, Abul K.
AU  - Appel, Silke
AU  - Bacchetta, Rosa
AU  - Battaglia, Manuela
AU  - Berglund, David
AU  - Blazar, Bruce
AU  - Bluestone, Jeffrey A.
AU  - Bornhäuser, Martin
AU  - Ten Brinke, Anja
AU  - Brusko, Todd M.
AU  - Cools, Nathalie
AU  - Cuturi, Maria Cristina
AU  - Geissler, Edward
AU  - Giannoukakis, Nick
AU  - Gołab, Karolina
AU  - Hafler, David A.
AU  - van Ham, S. Marieke
AU  - Hester, Joanna
AU  - Hippen, Keli
AU  - Di Ianni, Mauro
AU  - Radulović, Nataša
AU  - Isaacs, John
AU  - Issa, Fadi
AU  - Iwaszkiewicz-Grześ, Dorota
AU  - Jaeckel, Elmar
AU  - Joosten, Irma
AU  - Klatzmann, David
AU  - Koenen, Hans
AU  - van Kooten, Cees
AU  - Korsgren, Olle
AU  - Kretschmer, Karsten
AU  - Levings, Megan
AU  - Marek-Trzonkowska, Natalia Maria
AU  - Martinez-Llordella, Marc
AU  - Miljković, Đorđe
AU  - Mills, Kingston H G
AU  - Miranda, Joana P.
AU  - Piccirillo, Ciriaco A.
AU  - Putnam, Amy L.
AU  - Ritter, Thomas
AU  - Roncarolo, Maria Grazia
AU  - Sakaguchi, Shimon
AU  - Sánchez-Ramón, Silvia
AU  - Sawitzki, Birgit
AU  - Sofronic-Milosavljevic, Ljiljana
AU  - Sykes, Megan
AU  - Tang, Qizhi
AU  - Vives-Pi, Marta
AU  - Waldmann, Herman
AU  - Witkowski, Piotr
AU  - Wood, Kathryn J.
AU  - Gregori, Silvia
AU  - Hilkens, Catharien M. U.
AU  - Lombardi, Giovanna
AU  - Lord, Phillip
AU  - Martinez-Caceres, Eva M.
AU  - Trzonkowski, Piotr
PY  - 2018
UR  - http://journal.frontiersin.org/article/10.3389/fimmu.2017.01844/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5775516
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3539
AB  - Cellular therapies with CD4+ T regulatory cells (Tregs) hold promise of efficacious treatment for the variety of autoimmune and allergic diseases as well as posttransplant complications. Nevertheless, current manufacturing of Tregs as a cellular medicinal product varies between different laboratories, which in turn hampers precise comparisons of the results between the studies performed. While the number of clinical trials testing Tregs is already substantial, it seems to be crucial to provide some standardized characteristics of Treg products in order to minimize the problem. We have previously developed reporting guidelines called minimum information about tolerogenic antigen-presenting cells, which allows the comparison between different preparations of tolerance-inducing antigen-presenting cells. Having this experience, here we describe another minimum information about Tregs (MITREG). It is important to note that MITREG does not dictate how investigators should generate or characterize Tregs, but it does require investigators to report their Treg data in a consistent and transparent manner. We hope this will, therefore, be a useful tool facilitating standardized reporting on the manufacturing of Tregs, either for research purposes or for clinical application. This way MITREG might also be an important step toward more standardized and reproducible testing of the Tregs preparations in clinical applications.
T2  - Frontiers in Immunology
T1  - Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.
VL  - 8
DO  - 10.3389/fimmu.2017.01844
SP  - 1844
ER  - 

@article{
author = "Fuchs, Anke and Gliwiński, Mateusz and Grageda, Nathali and Spiering, Rachel and Abbas, Abul K. and Appel, Silke and Bacchetta, Rosa and Battaglia, Manuela and Berglund, David and Blazar, Bruce and Bluestone, Jeffrey A. and Bornhäuser, Martin and Ten Brinke, Anja and Brusko, Todd M. and Cools, Nathalie and Cuturi, Maria Cristina and Geissler, Edward and Giannoukakis, Nick and Gołab, Karolina and Hafler, David A. and van Ham, S. Marieke and Hester, Joanna and Hippen, Keli and Di Ianni, Mauro and Radulović, Nataša and Isaacs, John and Issa, Fadi and Iwaszkiewicz-Grześ, Dorota and Jaeckel, Elmar and Joosten, Irma and Klatzmann, David and Koenen, Hans and van Kooten, Cees and Korsgren, Olle and Kretschmer, Karsten and Levings, Megan and Marek-Trzonkowska, Natalia Maria and Martinez-Llordella, Marc and Miljković, Đorđe and Mills, Kingston H G and Miranda, Joana P. and Piccirillo, Ciriaco A. and Putnam, Amy L. and Ritter, Thomas and Roncarolo, Maria Grazia and Sakaguchi, Shimon and Sánchez-Ramón, Silvia and Sawitzki, Birgit and Sofronic-Milosavljevic, Ljiljana and Sykes, Megan and Tang, Qizhi and Vives-Pi, Marta and Waldmann, Herman and Witkowski, Piotr and Wood, Kathryn J. and Gregori, Silvia and Hilkens, Catharien M. U. and Lombardi, Giovanna and Lord, Phillip and Martinez-Caceres, Eva M. and Trzonkowski, Piotr",
year = "2018",
abstract = "Cellular therapies with CD4+ T regulatory cells (Tregs) hold promise of efficacious treatment for the variety of autoimmune and allergic diseases as well as posttransplant complications. Nevertheless, current manufacturing of Tregs as a cellular medicinal product varies between different laboratories, which in turn hampers precise comparisons of the results between the studies performed. While the number of clinical trials testing Tregs is already substantial, it seems to be crucial to provide some standardized characteristics of Treg products in order to minimize the problem. We have previously developed reporting guidelines called minimum information about tolerogenic antigen-presenting cells, which allows the comparison between different preparations of tolerance-inducing antigen-presenting cells. Having this experience, here we describe another minimum information about Tregs (MITREG). It is important to note that MITREG does not dictate how investigators should generate or characterize Tregs, but it does require investigators to report their Treg data in a consistent and transparent manner. We hope this will, therefore, be a useful tool facilitating standardized reporting on the manufacturing of Tregs, either for research purposes or for clinical application. This way MITREG might also be an important step toward more standardized and reproducible testing of the Tregs preparations in clinical applications.",
journal = "Frontiers in Immunology",
title = "Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.",
volume = "8",
doi = "10.3389/fimmu.2017.01844",
pages = "1844"
}

Fuchs, A., Gliwiński, M., Grageda, N., Spiering, R., Abbas, A. K., Appel, S., Bacchetta, R., Battaglia, M., Berglund, D., Blazar, B., Bluestone, J. A., Bornhäuser, M., Ten Brinke, A., Brusko, T. M., Cools, N., Cuturi, M. C., Geissler, E., Giannoukakis, N., Gołab, K., Hafler, D. A., van Ham, S. M., Hester, J., Hippen, K., Di Ianni, M., Radulović, N., Isaacs, J., Issa, F., Iwaszkiewicz-Grześ, D., Jaeckel, E., Joosten, I., Klatzmann, D., Koenen, H., van Kooten, C., Korsgren, O., Kretschmer, K., Levings, M., Marek-Trzonkowska, N. M., Martinez-Llordella, M., Miljković, Đ., Mills, K. H. G., Miranda, J. P., Piccirillo, C. A., Putnam, A. L., Ritter, T., Roncarolo, M. G., Sakaguchi, S., Sánchez-Ramón, S., Sawitzki, B., Sofronic-Milosavljevic, L., Sykes, M., Tang, Q., Vives-Pi, M., Waldmann, H., Witkowski, P., Wood, K. J., Gregori, S., Hilkens, C. M. U., Lombardi, G., Lord, P., Martinez-Caceres, E. M.,& Trzonkowski, P.. (2018). Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.. in Frontiers in Immunology, 8, 1844.
https://doi.org/10.3389/fimmu.2017.01844

Fuchs A, Gliwiński M, Grageda N, Spiering R, Abbas AK, Appel S, Bacchetta R, Battaglia M, Berglund D, Blazar B, Bluestone JA, Bornhäuser M, Ten Brinke A, Brusko TM, Cools N, Cuturi MC, Geissler E, Giannoukakis N, Gołab K, Hafler DA, van Ham SM, Hester J, Hippen K, Di Ianni M, Radulović N, Isaacs J, Issa F, Iwaszkiewicz-Grześ D, Jaeckel E, Joosten I, Klatzmann D, Koenen H, van Kooten C, Korsgren O, Kretschmer K, Levings M, Marek-Trzonkowska NM, Martinez-Llordella M, Miljković Đ, Mills KHG, Miranda JP, Piccirillo CA, Putnam AL, Ritter T, Roncarolo MG, Sakaguchi S, Sánchez-Ramón S, Sawitzki B, Sofronic-Milosavljevic L, Sykes M, Tang Q, Vives-Pi M, Waldmann H, Witkowski P, Wood KJ, Gregori S, Hilkens CMU, Lombardi G, Lord P, Martinez-Caceres EM, Trzonkowski P. Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.. in Frontiers in Immunology. 2018;8:1844.
doi:10.3389/fimmu.2017.01844 .

Fuchs, Anke, Gliwiński, Mateusz, Grageda, Nathali, Spiering, Rachel, Abbas, Abul K., Appel, Silke, Bacchetta, Rosa, Battaglia, Manuela, Berglund, David, Blazar, Bruce, Bluestone, Jeffrey A., Bornhäuser, Martin, Ten Brinke, Anja, Brusko, Todd M., Cools, Nathalie, Cuturi, Maria Cristina, Geissler, Edward, Giannoukakis, Nick, Gołab, Karolina, Hafler, David A., van Ham, S. Marieke, Hester, Joanna, Hippen, Keli, Di Ianni, Mauro, Radulović, Nataša, Isaacs, John, Issa, Fadi, Iwaszkiewicz-Grześ, Dorota, Jaeckel, Elmar, Joosten, Irma, Klatzmann, David, Koenen, Hans, van Kooten, Cees, Korsgren, Olle, Kretschmer, Karsten, Levings, Megan, Marek-Trzonkowska, Natalia Maria, Martinez-Llordella, Marc, Miljković, Đorđe, Mills, Kingston H G, Miranda, Joana P., Piccirillo, Ciriaco A., Putnam, Amy L., Ritter, Thomas, Roncarolo, Maria Grazia, Sakaguchi, Shimon, Sánchez-Ramón, Silvia, Sawitzki, Birgit, Sofronic-Milosavljevic, Ljiljana, Sykes, Megan, Tang, Qizhi, Vives-Pi, Marta, Waldmann, Herman, Witkowski, Piotr, Wood, Kathryn J., Gregori, Silvia, Hilkens, Catharien M. U., Lombardi, Giovanna, Lord, Phillip, Martinez-Caceres, Eva M., Trzonkowski, Piotr, "Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization." in Frontiers in Immunology, 8 (2018):1844,
https://doi.org/10.3389/fimmu.2017.01844 . .

TY  - JOUR
AU  - Stošić-Grujičić, Stanislava
AU  - Gruden-Movsesijan, Alisa
AU  - Radulović, Nataša
AU  - Mostarica-Stojković, Marija B
AU  - Milić, M
AU  - Sofronić-Milosavljević, Ljiljana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1373
AB  - P>Trichinella spiralis is a helminth that provokes Th2 and anti-inflammatory type responses in an infected host. Our previous studies using Dark Agouti (DA) rats indicated that T. spiralis infection reduced experimental autoimmune encephalomyelitis (EAE) severity in rats. The aim of this study was to analyse the mechanisms underlying EAE suppression driven by T. spiralis infection. Reduced clinical and histological manifestations of the disease were accompanied by increased IL-4 and IL-10 production and decreased IFN-gamma and IL-17 production in draining lymph node cells. This indicates that T. spiralis infection successfully maintains a Th2 cytokine bias regardless of EAE induction. High IL-10 signifies parasite-induced anti-inflammatory and/or regulatory cell responses. Transfer of splenic T cell-enriched population of cells from T. spiralis-infected rats into EAE immunized rats caused amelioration of EAE and in some cases protection from disease development. This population of cells contained higher proportion of CD4+ CD25+ Foxp3+ regulatory cells and produced high level of IL-10 when compared with uninfected rats.
T2  - Parasite Immunology
T1  - Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats
IS  - 6
VL  - 32
EP  - 459
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1373
ER  - 

@article{
author = "Stošić-Grujičić, Stanislava and Gruden-Movsesijan, Alisa and Radulović, Nataša and Mostarica-Stojković, Marija B and Milić, M and Sofronić-Milosavljević, Ljiljana",
year = "2010",
abstract = "P>Trichinella spiralis is a helminth that provokes Th2 and anti-inflammatory type responses in an infected host. Our previous studies using Dark Agouti (DA) rats indicated that T. spiralis infection reduced experimental autoimmune encephalomyelitis (EAE) severity in rats. The aim of this study was to analyse the mechanisms underlying EAE suppression driven by T. spiralis infection. Reduced clinical and histological manifestations of the disease were accompanied by increased IL-4 and IL-10 production and decreased IFN-gamma and IL-17 production in draining lymph node cells. This indicates that T. spiralis infection successfully maintains a Th2 cytokine bias regardless of EAE induction. High IL-10 signifies parasite-induced anti-inflammatory and/or regulatory cell responses. Transfer of splenic T cell-enriched population of cells from T. spiralis-infected rats into EAE immunized rats caused amelioration of EAE and in some cases protection from disease development. This population of cells contained higher proportion of CD4+ CD25+ Foxp3+ regulatory cells and produced high level of IL-10 when compared with uninfected rats.",
journal = "Parasite Immunology",
title = "Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats",
number = "6",
volume = "32",
pages = "459",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1373"
}

Stošić-Grujičić, S., Gruden-Movsesijan, A., Radulović, N., Mostarica-Stojković, M. B., Milić, M.,& Sofronić-Milosavljević, L.. (2010). Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats. in Parasite Immunology, 32(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1373

Stošić-Grujičić S, Gruden-Movsesijan A, Radulović N, Mostarica-Stojković MB, Milić M, Sofronić-Milosavljević L. Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats. in Parasite Immunology. 2010;32(6):null-459.
https://hdl.handle.net/21.15107/rcub_ibiss_1373 .

Stošić-Grujičić, Stanislava, Gruden-Movsesijan, Alisa, Radulović, Nataša, Mostarica-Stojković, Marija B, Milić, M, Sofronić-Milosavljević, Ljiljana, "Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats" in Parasite Immunology, 32, no. 6 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1373 .

TY  - JOUR
AU  - Gruden-Movsesijan, Alisa
AU  - Radulović, Nataša
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Milić, M
AU  - Sofronić-Milosavljević, Ljiljana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1537
AB  - Helminth infection has a potent systemic immunomodulatory effect on the host immune response, which also affects the development of autoimmune diseases. We investigated the dose-dependent influence of Trichinella spiralis infection on experimental autoimmune encephalomyelitis (EAE). Our model of concomitant T. spiralis infection and EAE demonstrates that established infection of Dark Agouti (DA) rats with the parasite causes amelioration of the clinical course of induced EAE in a dose-dependent way. Infection with T spiralis L1 stage muscle larvae (TSL1) reduced the severity of the autoimmune disease as judged by lower maximal clinical score, cumulative index, duration of illness and degree of mononuclear cell infiltration in T spiralis infected animals compared to control, EAE-induced group. This study provides a valuable model of worm infection to investigate helminth-induced regulatory mechanisms for optimal benefit to the host. (c) 2008 Elsevier Inc. All rights reserved.
T2  - Experimental Parasitology
T1  - Trichinella spiralis: Modulation of experimental autoimmune encephalomyelitis in DA rats
IS  - 4
VL  - 118
EP  - 647
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1537
ER  - 

@article{
author = "Gruden-Movsesijan, Alisa and Radulović, Nataša and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Milić, M and Sofronić-Milosavljević, Ljiljana",
year = "2008",
abstract = "Helminth infection has a potent systemic immunomodulatory effect on the host immune response, which also affects the development of autoimmune diseases. We investigated the dose-dependent influence of Trichinella spiralis infection on experimental autoimmune encephalomyelitis (EAE). Our model of concomitant T. spiralis infection and EAE demonstrates that established infection of Dark Agouti (DA) rats with the parasite causes amelioration of the clinical course of induced EAE in a dose-dependent way. Infection with T spiralis L1 stage muscle larvae (TSL1) reduced the severity of the autoimmune disease as judged by lower maximal clinical score, cumulative index, duration of illness and degree of mononuclear cell infiltration in T spiralis infected animals compared to control, EAE-induced group. This study provides a valuable model of worm infection to investigate helminth-induced regulatory mechanisms for optimal benefit to the host. (c) 2008 Elsevier Inc. All rights reserved.",
journal = "Experimental Parasitology",
title = "Trichinella spiralis: Modulation of experimental autoimmune encephalomyelitis in DA rats",
number = "4",
volume = "118",
pages = "647",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1537"
}

Gruden-Movsesijan, A., Radulović, N., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Milić, M.,& Sofronić-Milosavljević, L.. (2008). Trichinella spiralis: Modulation of experimental autoimmune encephalomyelitis in DA rats. in Experimental Parasitology, 118(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1537

Gruden-Movsesijan A, Radulović N, Mostarica-Stojković MB, Stošić-Grujičić S, Milić M, Sofronić-Milosavljević L. Trichinella spiralis: Modulation of experimental autoimmune encephalomyelitis in DA rats. in Experimental Parasitology. 2008;118(4):null-647.
https://hdl.handle.net/21.15107/rcub_ibiss_1537 .

Gruden-Movsesijan, Alisa, Radulović, Nataša, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Milić, M, Sofronić-Milosavljević, Ljiljana, "Trichinella spiralis: Modulation of experimental autoimmune encephalomyelitis in DA rats" in Experimental Parasitology, 118, no. 4 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1537 .