TY  - JOUR
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4892
AB  - The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.
PB  - Basel: MDPI
T2  - Insects
T1  - Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura
IS  - 2
VL  - 13
DO  - 10.3390/insects13020139
SP  - 139
ER  - 

@article{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2022",
abstract = "The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura",
number = "2",
volume = "13",
doi = "10.3390/insects13020139",
pages = "139"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2022). Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects
Basel: MDPI., 13(2), 139.
https://doi.org/10.3390/insects13020139

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects. 2022;13(2):139.
doi:10.3390/insects13020139 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura" in Insects, 13, no. 2 (2022):139,
https://doi.org/10.3390/insects13020139 . .

TY  - CONF
AU  - Rakić, Mina
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
AU  - Jelić, Mihailo
AU  - Lapčević, Simona
AU  - Savić Veselinović, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5496
AB  - Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Lead induces sex and development related differential Mtn gene expression in two Drosophila species
SP  - 471
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5496
ER  - 

@conference{
author = "Rakić, Mina and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija and Jelić, Mihailo and Lapčević, Simona and Savić Veselinović, Marija",
year = "2022",
abstract = "Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Lead induces sex and development related differential Mtn gene expression in two Drosophila species",
pages = "471",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5496"
}

Rakić, M., Patenković, A., Stamenković-Radak, M., Tanasković, M., Jelić, M., Lapčević, S.,& Savić Veselinović, M.. (2022). Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
European Society for Evolutionary Biology., 471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496

Rakić M, Patenković A, Stamenković-Radak M, Tanasković M, Jelić M, Lapčević S, Savić Veselinović M. Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .

Rakić, Mina, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, Jelić, Mihailo, Lapčević, Simona, Savić Veselinović, Marija, "Lead induces sex and development related differential Mtn gene expression in two Drosophila species" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):471,
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .

TY  - CONF
AU  - Savić Veselinović, Marija
AU  - Rakić, Mina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5495
AB  - Повећање концентрација тешких метала у животној средини код различитих
организама може довести до поремећаја равнотеже концентрација есенцијалних
метала, узрокујући њихову токсичност као и неесенцијални метали. Иако су многи
од механизама који су у основи одговора на овај тип стреса прилично проучени,
присутна је варијабилност међу таксономским групама. Једна од првих линија
одбране већине организама јесте повећана експресија гена за металотионеине чији
су протеински продукти кратки, богати цистеинима, којима могу везивати јоне
метала и на тај начин вршити детоксикацију. Међу инсектима постоје врсте које
имају само један ген за металотионеин, али и оне које имају већи број гена који су
настали дупликацијама током еволуције. Функционалност и специфичност у
везивању одређених метала металотионеинима још увек није потпуно истражена, с
обзиром да се новим бионформатичким анализама и даље откривају нови гени код
неких од њих. Код рода Drosophila је скоро откривен шести ген за металотионеине
(MtnF), који би на основу секвенце требало да кодира протеин богатији цистеином
у поређењу са преосталих пет (Mtn A, B, C, D, E). Функција ових гена је у нашем
истраживању испитивана код две врсте овог рода, при изложености третманима
различитим металима и њиховим концентрацијама, различитог трајања и у
различитим стадијумима развића. Резултати анализе експресије гена MtnF не
указују на претпостављену специфичност везивања одређених метала.
AB  - Povećanje koncentracija teških metala u životnoj sredini kod različitih organizama može dovesti do poremećaja ravnoteže koncentracija esencijalnih metala, uzrokujući njihovu toksičnost kao i neesencijalni metali. Iako su mnogi od mehanizama koji su u osnovi odgovora na ovaj tip stresa prilično proučeni, prisutna je varijabilnost među taksonomskim grupama. Jedna od prvih linija odbrane većine organizama jeste povećana ekspresija gena za metalotioneine čiji su proteinski produkti kratki, bogati cisteinima, kojima mogu vezivati jone metala i na taj način vršiti detoksikaciju. Među insektima postoje vrste koje imaju samo jedan gen za metalotionein, ali i one koje imaju veći broj gena koji su nastali duplikacijama tokom evolucije. Funkcionalnost i specifičnost u vezivanju određenih metala metalotioneinima još uvek nije potpuno istražena, s obzirom da se novim bionformatičkim analizama i dalje otkrivaju novi geni kod nekih od njih. Kod roda Drosophila je skoro otkriven šesti gen za metalotioneine (MtnF), koji bi na osnovu sekvence trebalo da kodira protein bogatiji cisteinom u poređenju sa preostalih pet (Mtn A, B, C, D, E). Funkcija ovih gena je u našem istraživanju ispitivana kod dve vrste ovog roda, pri izloženosti tretmanima različitim metalima i njihovim koncentracijama, različitog trajanja i u različitim stadijumima razvića. Rezultati analize ekspresije gena MtnF ne ukazuju na pretpostavljenu specifičnost vezivanja određenih metala.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине
T1  - Odgovor na stres indukovan metalima kod insekata – evolucija i uloga gena za metalotioneine
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5495
ER  - 

@conference{
author = "Savić Veselinović, Marija and Rakić, Mina and Patenković, Aleksandra and Tanasković, Marija and Jelić, Mihailo and Stamenković-Radak, Marina",
year = "2022",
abstract = "Повећање концентрација тешких метала у животној средини код различитих
организама може довести до поремећаја равнотеже концентрација есенцијалних
метала, узрокујући њихову токсичност као и неесенцијални метали. Иако су многи
од механизама који су у основи одговора на овај тип стреса прилично проучени,
присутна је варијабилност међу таксономским групама. Једна од првих линија
одбране већине организама јесте повећана експресија гена за металотионеине чији
су протеински продукти кратки, богати цистеинима, којима могу везивати јоне
метала и на тај начин вршити детоксикацију. Међу инсектима постоје врсте које
имају само један ген за металотионеин, али и оне које имају већи број гена који су
настали дупликацијама током еволуције. Функционалност и специфичност у
везивању одређених метала металотионеинима још увек није потпуно истражена, с
обзиром да се новим бионформатичким анализама и даље откривају нови гени код
неких од њих. Код рода Drosophila је скоро откривен шести ген за металотионеине
(MtnF), који би на основу секвенце требало да кодира протеин богатији цистеином
у поређењу са преосталих пет (Mtn A, B, C, D, E). Функција ових гена је у нашем
истраживању испитивана код две врсте овог рода, при изложености третманима
различитим металима и њиховим концентрацијама, различитог трајања и у
различитим стадијумима развића. Резултати анализе експресије гена MtnF не
указују на претпостављену специфичност везивања одређених метала., Povećanje koncentracija teških metala u životnoj sredini kod različitih organizama može dovesti do poremećaja ravnoteže koncentracija esencijalnih metala, uzrokujući njihovu toksičnost kao i neesencijalni metali. Iako su mnogi od mehanizama koji su u osnovi odgovora na ovaj tip stresa prilično proučeni, prisutna je varijabilnost među taksonomskim grupama. Jedna od prvih linija odbrane većine organizama jeste povećana ekspresija gena za metalotioneine čiji su proteinski produkti kratki, bogati cisteinima, kojima mogu vezivati jone metala i na taj način vršiti detoksikaciju. Među insektima postoje vrste koje imaju samo jedan gen za metalotionein, ali i one koje imaju veći broj gena koji su nastali duplikacijama tokom evolucije. Funkcionalnost i specifičnost u vezivanju određenih metala metalotioneinima još uvek nije potpuno istražena, s obzirom da se novim bionformatičkim analizama i dalje otkrivaju novi geni kod nekih od njih. Kod roda Drosophila je skoro otkriven šesti gen za metalotioneine (MtnF), koji bi na osnovu sekvence trebalo da kodira protein bogatiji cisteinom u poređenju sa preostalih pet (Mtn A, B, C, D, E). Funkcija ovih gena je u našem istraživanju ispitivana kod dve vrste ovog roda, pri izloženosti tretmanima različitim metalima i njihovim koncentracijama, različitog trajanja i u različitim stadijumima razvića. Rezultati analize ekspresije gena MtnF ne ukazuju na pretpostavljenu specifičnost vezivanja određenih metala.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине, Odgovor na stres indukovan metalima kod insekata – evolucija i uloga gena za metalotioneine",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5495"
}

Savić Veselinović, M., Rakić, M., Patenković, A., Tanasković, M., Jelić, M.,& Stamenković-Radak, M.. (2022). Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 88.
https://hdl.handle.net/21.15107/rcub_ibiss_5495

Savić Veselinović M, Rakić M, Patenković A, Tanasković M, Jelić M, Stamenković-Radak M. Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:88.
https://hdl.handle.net/21.15107/rcub_ibiss_5495 .

Savić Veselinović, Marija, Rakić, Mina, Patenković, Aleksandra, Tanasković, Marija, Jelić, Mihailo, Stamenković-Radak, Marina, "Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):88,
https://hdl.handle.net/21.15107/rcub_ibiss_5495 .

TY  - CONF
AU  - Rakić, Mina
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Savić Veselinović, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4923
AB  - Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures
DO  - 10.3390/IECE-10508
SP  - 10508
ER  - 

@conference{
author = "Rakić, Mina and Jelić, Mihailo and Stamenković-Radak, Marina and Patenković, Aleksandra and Tanasković, Marija and Erić, Katarina and Davidović, Slobodan and Erić, Pavle and Savić Veselinović, Marija",
year = "2021",
abstract = "Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures",
doi = "10.3390/IECE-10508",
pages = "10508"
}

Rakić, M., Jelić, M., Stamenković-Radak, M., Patenković, A., Tanasković, M., Erić, K., Davidović, S., Erić, P.,& Savić Veselinović, M.. (2021). Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10508.
https://doi.org/10.3390/IECE-10508

Rakić M, Jelić M, Stamenković-Radak M, Patenković A, Tanasković M, Erić K, Davidović S, Erić P, Savić Veselinović M. Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10508.
doi:10.3390/IECE-10508 .

Rakić, Mina, Jelić, Mihailo, Stamenković-Radak, Marina, Patenković, Aleksandra, Tanasković, Marija, Erić, Katarina, Davidović, Slobodan, Erić, Pavle, Savić Veselinović, Marija, "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10508,
https://doi.org/10.3390/IECE-10508 . .

TY  - CONF
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4894
AB  - Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura
DO  - 10.3390/IECE-10522
SP  - 10522
ER  - 

@conference{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2021",
abstract = "Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura",
doi = "10.3390/IECE-10522",
pages = "10522"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2021). Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10522.
https://doi.org/10.3390/IECE-10522

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10522.
doi:10.3390/IECE-10522 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10522,
https://doi.org/10.3390/IECE-10522 . .