TY  - JOUR
AU  - Kojić, Danijela
AU  - Popović, Željko D.
AU  - Orčić, Dejan
AU  - Purać, Jelena
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira L.
AU  - Nikolić, Tatjana V.
AU  - Blagojević, Duško
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0022191018300921?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3116
AB  - The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.
T2  - Journal of insect physiology
T1  - The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).
VL  - 109
DO  - 10.1016/j.jinsphys.2018.07.007
SP  - 107
EP  - 113
ER  - 

@article{
author = "Kojić, Danijela and Popović, Željko D. and Orčić, Dejan and Purać, Jelena and Orčić, Snežana and Vukašinović, Elvira L. and Nikolić, Tatjana V. and Blagojević, Duško",
year = "2018",
abstract = "The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.",
journal = "Journal of insect physiology",
title = "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).",
volume = "109",
doi = "10.1016/j.jinsphys.2018.07.007",
pages = "107-113"
}

Kojić, D., Popović, Ž. D., Orčić, D., Purać, J., Orčić, S., Vukašinović, E. L., Nikolić, T. V.,& Blagojević, D.. (2018). The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology, 109, 107-113.
https://doi.org/10.1016/j.jinsphys.2018.07.007

Kojić D, Popović ŽD, Orčić D, Purać J, Orčić S, Vukašinović EL, Nikolić TV, Blagojević D. The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology. 2018;109:107-113.
doi:10.1016/j.jinsphys.2018.07.007 .

Kojić, Danijela, Popović, Željko D., Orčić, Dejan, Purać, Jelena, Orčić, Snežana, Vukašinović, Elvira L., Nikolić, Tatjana V., Blagojević, Duško, "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.)." in Journal of insect physiology, 109 (2018):107-113,
https://doi.org/10.1016/j.jinsphys.2018.07.007 . .