TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bajić, Aleksandar
AU  - Bjelobaba, Ivana
AU  - Milosević, Milena B
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Clarner, Tim
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/958
AB  - Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.
T2  - Journal of Molecular Neuroscience
T1  - Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro
IS  - 3
VL  - 51
SP  - 23
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_958
ER  - 

@article{
author = "Brisevac, Dusica and Bajić, Aleksandar and Bjelobaba, Ivana and Milosević, Milena B and Stojiljković, Mirjana B and Beyer, Cordian and Clarner, Tim and Kipp, Markus and Nedeljković, Nadezda N",
year = "2013",
abstract = "Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.",
journal = "Journal of Molecular Neuroscience",
title = "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro",
number = "3",
volume = "51",
pages = "23-879",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_958"
}

Brisevac, D., Bajić, A., Bjelobaba, I., Milosević, M. B., Stojiljković, M. B., Beyer, C., Clarner, T., Kipp, M.,& Nedeljković, N. N.. (2013). Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience, 51(3), 23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958

Brisevac D, Bajić A, Bjelobaba I, Milosević MB, Stojiljković MB, Beyer C, Clarner T, Kipp M, Nedeljković NN. Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience. 2013;51(3):23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

Brisevac, Dusica, Bajić, Aleksandar, Bjelobaba, Ivana, Milosević, Milena B, Stojiljković, Mirjana B, Beyer, Cordian, Clarner, Tim, Kipp, Markus, Nedeljković, Nadezda N, "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro" in Journal of Molecular Neuroscience, 51, no. 3 (2013):23-879,
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bjelobaba, Ivana
AU  - Bajić, Aleksandar
AU  - Clarner, Tim
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Anđus, Pavle R
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1098
AB  - Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors
IS  - 5
VL  - 61
SP  - 406
EP  - 688
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1098
ER  - 

@article{
author = "Brisevac, Dusica and Bjelobaba, Ivana and Bajić, Aleksandar and Clarner, Tim and Stojiljković, Mirjana B and Beyer, Cordian and Anđus, Pavle R and Kipp, Markus and Nedeljković, Nadezda N",
year = "2012",
abstract = "Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors",
number = "5",
volume = "61",
pages = "406-688",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1098"
}

Brisevac, D., Bjelobaba, I., Bajić, A., Clarner, T., Stojiljković, M. B., Beyer, C., Anđus, P. R., Kipp, M.,& Nedeljković, N. N.. (2012). Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International, 61(5), 406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098

Brisevac D, Bjelobaba I, Bajić A, Clarner T, Stojiljković MB, Beyer C, Anđus PR, Kipp M, Nedeljković NN. Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International. 2012;61(5):406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .

Brisevac, Dusica, Bjelobaba, Ivana, Bajić, Aleksandar, Clarner, Tim, Stojiljković, Mirjana B, Beyer, Cordian, Anđus, Pavle R, Kipp, Markus, Nedeljković, Nadezda N, "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors" in Neurochemistry International, 61, no. 5 (2012):406-688,
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .