TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bajić, Aleksandar
AU  - Bjelobaba, Ivana
AU  - Milosević, Milena B
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Clarner, Tim
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/958
AB  - Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.
T2  - Journal of Molecular Neuroscience
T1  - Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro
IS  - 3
VL  - 51
SP  - 23
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_958
ER  - 

@article{
author = "Brisevac, Dusica and Bajić, Aleksandar and Bjelobaba, Ivana and Milosević, Milena B and Stojiljković, Mirjana B and Beyer, Cordian and Clarner, Tim and Kipp, Markus and Nedeljković, Nadezda N",
year = "2013",
abstract = "Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.",
journal = "Journal of Molecular Neuroscience",
title = "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro",
number = "3",
volume = "51",
pages = "23-879",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_958"
}

Brisevac, D., Bajić, A., Bjelobaba, I., Milosević, M. B., Stojiljković, M. B., Beyer, C., Clarner, T., Kipp, M.,& Nedeljković, N. N.. (2013). Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience, 51(3), 23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958

Brisevac D, Bajić A, Bjelobaba I, Milosević MB, Stojiljković MB, Beyer C, Clarner T, Kipp M, Nedeljković NN. Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience. 2013;51(3):23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

Brisevac, Dusica, Bajić, Aleksandar, Bjelobaba, Ivana, Milosević, Milena B, Stojiljković, Mirjana B, Beyer, Cordian, Clarner, Tim, Kipp, Markus, Nedeljković, Nadezda N, "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro" in Journal of Molecular Neuroscience, 51, no. 3 (2013):23-879,
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

TY  - JOUR
AU  - Bataveljić, Danijela B
AU  - Nikolić, Ljiljana
AU  - Milosević, Milena B
AU  - Todorović, Nataša
AU  - Anđus, Pavle R
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1083
AB  - Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting upper and lower motor neurons. Dysfunction and death of motor neurons are closely related to the modified astrocytic environment. Astrocytic endfeet, lining the bloodbrain barrier (BBB), are enriched in two proteins, aquaporin-4 (AQP4) and inwardly rectifying potassium channel (Kir) 4.1. Both channels are important for the maintainance of a functional BBB astrocytic lining. In this study, expression levels of AQP4 and Kir4.1 were for the first time examined in the brainstem and cortex, along with the functional properties of Kir channels in cultured cortical astrocytes of the SOD1G93A rat model of ALS. Western blot analysis showed increased expression of AQP4 and decreased expression of Kir4.1 in the brainstem and cortex of the ALS rat. In addition, higher immunoreactivity of AQP4 and reduced immunolabeling of Kir4.1 in facial and trigeminal nuclei as well as in the motor cortex were also observed. Particularly, the observed changes in the expression of both channels were retained in cultured astrocytes. Furthermore, whole-cell patch-clamp recordings from cultured ALS cortical astrocytes showed a significantly lower Kir current density. Importantly, the potassium uptake current in ALS astrocytes was significantly reduced at all extracellular potassium concentrations. Consequently, the Kir-specific Cs+- and Ba2+-sensitive currents were also decreased. The changes in the studied channels, notably at the upper CNS level, could underline the hampered ability of astrocytes to maintain water and potassium homeostasis, thus affecting the BBB, disturbing the neuronal microenvironment, and causing motoneuronal dysfunction and death. (c) 2012 Wiley Periodicals, Inc.
PB  - Wiley
T2  - Glia
T1  - Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model
IS  - 12
VL  - 60
DO  - 10.1002/glia.22414
SP  - 1991
EP  - 2003
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1083
ER  - 

@article{
author = "Bataveljić, Danijela B and Nikolić, Ljiljana and Milosević, Milena B and Todorović, Nataša and Anđus, Pavle R",
year = "2012",
abstract = "Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting upper and lower motor neurons. Dysfunction and death of motor neurons are closely related to the modified astrocytic environment. Astrocytic endfeet, lining the bloodbrain barrier (BBB), are enriched in two proteins, aquaporin-4 (AQP4) and inwardly rectifying potassium channel (Kir) 4.1. Both channels are important for the maintainance of a functional BBB astrocytic lining. In this study, expression levels of AQP4 and Kir4.1 were for the first time examined in the brainstem and cortex, along with the functional properties of Kir channels in cultured cortical astrocytes of the SOD1G93A rat model of ALS. Western blot analysis showed increased expression of AQP4 and decreased expression of Kir4.1 in the brainstem and cortex of the ALS rat. In addition, higher immunoreactivity of AQP4 and reduced immunolabeling of Kir4.1 in facial and trigeminal nuclei as well as in the motor cortex were also observed. Particularly, the observed changes in the expression of both channels were retained in cultured astrocytes. Furthermore, whole-cell patch-clamp recordings from cultured ALS cortical astrocytes showed a significantly lower Kir current density. Importantly, the potassium uptake current in ALS astrocytes was significantly reduced at all extracellular potassium concentrations. Consequently, the Kir-specific Cs+- and Ba2+-sensitive currents were also decreased. The changes in the studied channels, notably at the upper CNS level, could underline the hampered ability of astrocytes to maintain water and potassium homeostasis, thus affecting the BBB, disturbing the neuronal microenvironment, and causing motoneuronal dysfunction and death. (c) 2012 Wiley Periodicals, Inc.",
publisher = "Wiley",
journal = "Glia",
title = "Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model",
number = "12",
volume = "60",
doi = "10.1002/glia.22414",
pages = "1991-2003",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1083"
}

Bataveljić, D. B., Nikolić, L., Milosević, M. B., Todorović, N.,& Anđus, P. R.. (2012). Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model. in Glia
Wiley., 60(12), 1991-2003.
https://doi.org/10.1002/glia.22414
https://hdl.handle.net/21.15107/rcub_ibiss_1083

Bataveljić DB, Nikolić L, Milosević MB, Todorović N, Anđus PR. Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model. in Glia. 2012;60(12):1991-2003.
doi:10.1002/glia.22414
https://hdl.handle.net/21.15107/rcub_ibiss_1083 .

Bataveljić, Danijela B, Nikolić, Ljiljana, Milosević, Milena B, Todorović, Nataša, Anđus, Pavle R, "Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model" in Glia, 60, no. 12 (2012):1991-2003,
https://doi.org/10.1002/glia.22414 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1083 .