TY  - JOUR
AU  - Balusu, Sriram
AU  - Van Wonterghem, Elien
AU  - De Rycke, Riet
AU  - Raemdonck, Koen
AU  - Stremersch, Stephan
AU  - Gevaert, Kris
AU  - Brkić, Marjana
AU  - Demeestere, Delphine
AU  - Vanhooren, Valerie
AU  - Hendrix, An
AU  - Libert, Claude
AU  - Vandenbroucke, Roosmarijn E.
PY  - 2016
UR  - http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201606271
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84989915450&origin=SingleRecordEmailAlert&txGid=66B9A38F509F4540CF41E07DD21FB6C0.wsnAw8kcdt7IPYLO0V48gA:27#
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2471
AB  - Here, we identified release of extracellular vesicles (EVs) by the choroid plexus epithelium (CPE) as a new mechanism of blood–brain communication. Systemic inflammation induced an increase in EVs and associated pro-inflammatory miRNAs, including miR-146a and miR-155, in the CSF. Interestingly, this was associated with an increase in amount of multivesicular bodies (MVBs) and exosomes per MVB in the CPE cells. Additionally, we could mimic this using LPS-stimulated primary CPE cells and choroid plexus explants. These choroid plexus-derived EVs can enter the brain parenchyma and are taken up by astrocytes and microglia, inducing miRNA target repression and inflammatory gene up-regulation. Interestingly, this could be blocked in vivo by intracerebroventricular (icv) injection of an inhibitor of exosome production. Our data show that CPE cells sense and transmit information about the peripheral inflammatory status to the central nervous system (CNS) via the release of EVs into the CSF, which transfer this pro-inflammatory message to recipient brain cells. Additionally, we revealed that blockage of EV secretion decreases brain inflammation, which opens up new avenues to treat systemic inflammatory diseases such as sepsis.
PB  - Blackwell Publishing Ltd
T2  - EMBO Molecular Medicine
T1  - Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles
IS  - 10
VL  - 8
DO  - 10.15252/emmm.201606271
SP  - 1162
EP  - 1183
ER  - 

@article{
author = "Balusu, Sriram and Van Wonterghem, Elien and De Rycke, Riet and Raemdonck, Koen and Stremersch, Stephan and Gevaert, Kris and Brkić, Marjana and Demeestere, Delphine and Vanhooren, Valerie and Hendrix, An and Libert, Claude and Vandenbroucke, Roosmarijn E.",
year = "2016",
abstract = "Here, we identified release of extracellular vesicles (EVs) by the choroid plexus epithelium (CPE) as a new mechanism of blood–brain communication. Systemic inflammation induced an increase in EVs and associated pro-inflammatory miRNAs, including miR-146a and miR-155, in the CSF. Interestingly, this was associated with an increase in amount of multivesicular bodies (MVBs) and exosomes per MVB in the CPE cells. Additionally, we could mimic this using LPS-stimulated primary CPE cells and choroid plexus explants. These choroid plexus-derived EVs can enter the brain parenchyma and are taken up by astrocytes and microglia, inducing miRNA target repression and inflammatory gene up-regulation. Interestingly, this could be blocked in vivo by intracerebroventricular (icv) injection of an inhibitor of exosome production. Our data show that CPE cells sense and transmit information about the peripheral inflammatory status to the central nervous system (CNS) via the release of EVs into the CSF, which transfer this pro-inflammatory message to recipient brain cells. Additionally, we revealed that blockage of EV secretion decreases brain inflammation, which opens up new avenues to treat systemic inflammatory diseases such as sepsis.",
publisher = "Blackwell Publishing Ltd",
journal = "EMBO Molecular Medicine",
title = "Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles",
number = "10",
volume = "8",
doi = "10.15252/emmm.201606271",
pages = "1162-1183"
}

Balusu, S., Van Wonterghem, E., De Rycke, R., Raemdonck, K., Stremersch, S., Gevaert, K., Brkić, M., Demeestere, D., Vanhooren, V., Hendrix, A., Libert, C.,& Vandenbroucke, R. E.. (2016). Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles. in EMBO Molecular Medicine
Blackwell Publishing Ltd., 8(10), 1162-1183.
https://doi.org/10.15252/emmm.201606271

Balusu S, Van Wonterghem E, De Rycke R, Raemdonck K, Stremersch S, Gevaert K, Brkić M, Demeestere D, Vanhooren V, Hendrix A, Libert C, Vandenbroucke RE. Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles. in EMBO Molecular Medicine. 2016;8(10):1162-1183.
doi:10.15252/emmm.201606271 .

Balusu, Sriram, Van Wonterghem, Elien, De Rycke, Riet, Raemdonck, Koen, Stremersch, Stephan, Gevaert, Kris, Brkić, Marjana, Demeestere, Delphine, Vanhooren, Valerie, Hendrix, An, Libert, Claude, Vandenbroucke, Roosmarijn E., "Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles" in EMBO Molecular Medicine, 8, no. 10 (2016):1162-1183,
https://doi.org/10.15252/emmm.201606271 . .