TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2022
UR  - https://www.mdpi.com/2072-6643/14/6/1273
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35334928
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8955508
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4940
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.
IS  - 6
VL  - 14
DO  - 10.3390/nu14061273
SP  - 1273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.",
number = "6",
volume = "14",
doi = "10.3390/nu14061273",
pages = "1273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Božić Nedeljković, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients
Basel: MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Božić Nedeljković B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis." in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacić, Sanja
AU  - Repac, Jelena
AU  - Drašković-Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/22/5426
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4034
AB  - Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.
PB  - NLM (Medline)
T2  - Molecules
T1  - Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury
IS  - 22
VL  - 25
DO  - 10.3390/molecules25225426
SP  - 5426
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacić, Sanja and Repac, Jelena and Drašković-Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury",
number = "22",
volume = "25",
doi = "10.3390/molecules25225426",
pages = "5426"
}

Ehmedah, A., Nedeljković, P., Dacić, S., Repac, J., Drašković-Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2020). Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules
NLM (Medline)., 25(22), 5426.
https://doi.org/10.3390/molecules25225426

Ehmedah A, Nedeljković P, Dacić S, Repac J, Drašković-Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules. 2020;25(22):5426.
doi:10.3390/molecules25225426 .

Ehmedah, Adil, Nedeljković, Predrag, Dacić, Sanja, Repac, Jelena, Drašković-Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury" in Molecules, 25, no. 22 (2020):5426,
https://doi.org/10.3390/molecules25225426 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacic, Sanja
AU  - Repac, Jelena
AU  - Drašković Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4615
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3578
AB  - Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244615
SP  - 4615
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacic, Sanja and Repac, Jelena and Drašković Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2019",
abstract = "Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.",
number = "24",
volume = "24",
doi = "10.3390/molecules24244615",
pages = "4615"
}

Ehmedah, A., Nedeljković, P., Dacic, S., Repac, J., Drašković Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2019). Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4615.
https://doi.org/10.3390/molecules24244615

Ehmedah A, Nedeljković P, Dacic S, Repac J, Drašković Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland). 2019;24(24):4615.
doi:10.3390/molecules24244615 .

Ehmedah, Adil, Nedeljković, Predrag, Dacic, Sanja, Repac, Jelena, Drašković Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4615,
https://doi.org/10.3390/molecules24244615 . .