TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622003723
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5174
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
SP  - 105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302",
pages = "105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods, 99, 105302.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99:105302.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022):105302,
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Blagojević, Duško
AU  - Šobajić, Slađana
AU  - Čolić, Miodrag
AU  - Vasiljevska, Milijana
AU  - Poznanić, Milica
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/37
AB  - The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons.
AB  - Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.
T2  - Jugoslovenska medicinska biohemija
T1  - Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme
T1  - The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons
IS  - 1
VL  - 24
SP  - 15
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_37
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Blagojević, Duško and Šobajić, Slađana and Čolić, Miodrag and Vasiljevska, Milijana and Poznanić, Milica",
year = "2005, 2005",
abstract = "The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons., Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.",
journal = "Jugoslovenska medicinska biohemija",
title = "Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme, The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons",
number = "1",
volume = "24",
pages = "15-20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_37"
}

Cerović, A., Miletić, I., Blagojević, D., Šobajić, S., Čolić, M., Vasiljevska, M.,& Poznanić, M.. (2005). Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme. in Jugoslovenska medicinska biohemija, 24(1), 15-20.
https://hdl.handle.net/21.15107/rcub_ibiss_37

Cerović A, Miletić I, Blagojević D, Šobajić S, Čolić M, Vasiljevska M, Poznanić M. Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme. in Jugoslovenska medicinska biohemija. 2005;24(1):15-20.
https://hdl.handle.net/21.15107/rcub_ibiss_37 .

Cerović, Aleksandra, Miletić, Ivanka, Blagojević, Duško, Šobajić, Slađana, Čolić, Miodrag, Vasiljevska, Milijana, Poznanić, Milica, "Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme" in Jugoslovenska medicinska biohemija, 24, no. 1 (2005):15-20,
https://hdl.handle.net/21.15107/rcub_ibiss_37 .