TY  - JOUR
AU  - Radić, I
AU  - Mijović, M
AU  - Tatalović, Nikola
AU  - Mitić, M
AU  - Lukić, V
AU  - Joksimović, B
AU  - Petrović, Z
AU  - Ristić, S
AU  - Veličković, S
AU  - Nestorovic, V
AU  - Corac, A
AU  - Mirić, M
AU  - Adžić, M
AU  - Blagojević, Duško
AU  - Popović, L
AU  - Hudomal, S J
PY  - 2019
UR  - http://journals.sagepub.com/doi/10.1177/0960327119829518
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3281
AB  - In 2012, alcohol liver disease resulted in 3.3 million-5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey ( per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper-zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.
T2  - Human & Experimental Toxicology
T1  - Protective effects of whey on rat liver damage induced by chronic alcohol intake.
DO  - 10.1177/0960327119829518
ER  - 

@article{
author = "Radić, I and Mijović, M and Tatalović, Nikola and Mitić, M and Lukić, V and Joksimović, B and Petrović, Z and Ristić, S and Veličković, S and Nestorovic, V and Corac, A and Mirić, M and Adžić, M and Blagojević, Duško and Popović, L and Hudomal, S J",
year = "2019",
abstract = "In 2012, alcohol liver disease resulted in 3.3 million-5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey ( per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper-zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.",
journal = "Human & Experimental Toxicology",
title = "Protective effects of whey on rat liver damage induced by chronic alcohol intake.",
doi = "10.1177/0960327119829518"
}

Radić, I., Mijović, M., Tatalović, N., Mitić, M., Lukić, V., Joksimović, B., Petrović, Z., Ristić, S., Veličković, S., Nestorovic, V., Corac, A., Mirić, M., Adžić, M., Blagojević, D., Popović, L.,& Hudomal, S. J.. (2019). Protective effects of whey on rat liver damage induced by chronic alcohol intake.. in Human & Experimental Toxicology.
https://doi.org/10.1177/0960327119829518

Radić I, Mijović M, Tatalović N, Mitić M, Lukić V, Joksimović B, Petrović Z, Ristić S, Veličković S, Nestorovic V, Corac A, Mirić M, Adžić M, Blagojević D, Popović L, Hudomal SJ. Protective effects of whey on rat liver damage induced by chronic alcohol intake.. in Human & Experimental Toxicology. 2019;.
doi:10.1177/0960327119829518 .

Radić, I, Mijović, M, Tatalović, Nikola, Mitić, M, Lukić, V, Joksimović, B, Petrović, Z, Ristić, S, Veličković, S, Nestorovic, V, Corac, A, Mirić, M, Adžić, M, Blagojević, Duško, Popović, L, Hudomal, S J, "Protective effects of whey on rat liver damage induced by chronic alcohol intake." in Human & Experimental Toxicology (2019),
https://doi.org/10.1177/0960327119829518 . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Todorović, Danijela
AU  - Veličković, Miroslava
AU  - Ušćebrka, Gordana
AU  - Knežević, Veroljub S.
AU  - Ajdžanović, Vladimir
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/497
AB  - Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.
AB  - Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.
T2  - Journal of Medical Biochemistry
T1  - Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom
T1  - Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin
IS  - 1
VL  - 31
SP  - 34
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_497
ER  - 

@article{
author = "Milošević, Verica and Ristić, Nataša and Todorović, Danijela and Veličković, Miroslava and Ušćebrka, Gordana and Knežević, Veroljub S. and Ajdžanović, Vladimir",
year = "2012, 2012",
abstract = "Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG., Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.",
journal = "Journal of Medical Biochemistry",
title = "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom, Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin",
number = "1",
volume = "31",
pages = "34-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_497"
}

Milošević, V., Ristić, N., Todorović, D., Veličković, M., Ušćebrka, G., Knežević, V. S.,& Ajdžanović, V.. (2012). Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry, 31(1), 34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497

Milošević V, Ristić N, Todorović D, Veličković M, Ušćebrka G, Knežević VS, Ajdžanović V. Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry. 2012;31(1):34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497 .

Milošević, Verica, Ristić, Nataša, Todorović, Danijela, Veličković, Miroslava, Ušćebrka, Gordana, Knežević, Veroljub S., Ajdžanović, Vladimir, "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom" in Journal of Medical Biochemistry, 31, no. 1 (2012):34-39,
https://hdl.handle.net/21.15107/rcub_ibiss_497 .