@article{
author = "Matias, Diogo and Nicolai, Marisa and Saraiva, Lucília and Pinheiro, Rute and Faustino, Célia and Diaz Lanza, Ana and Pinto Reis, Catarina and Stanković, Tijana and Dinić, Jelena and Pešić, Milica and Rijo, Patrícia",
year = "2019",
abstract = "Cytotoxicity screenings have identified Plectranthus plants as potential sources of antitumor lead compounds. In this work, several extracts from Plectranthus madagascariensis were prepared using different solvents (acetone, methanol, and supercritical CO2) and extraction techniques (maceration, ultrasound-assisted, and supercritical fluid extraction), and their chemical composition was detailed using high-performance liquid chromatography with a diode array detector. The cytotoxic activity of the major compounds identified, namely, rosmarinic acid (1) and abietane diterpenes 7α,6β-dihydroxyroyleanone (2), 7α-formyloxy-6β-hydroxyroyleanone (3), 7α-acetoxy-6β-hydroxyroyleanone (4), and coleon U (5), was evaluated in a battery of human cancer cell lines, including breast (MDA-MB-231, MCF-7), colon (HCT116), and lung (NCI-H460, NCI-H460/R) cancer, and also in healthy lung (MCR-5) cells. Royleanone (3) was isolated for the first time from P. madagascariensis, and its full spectroscopic characterization (proto...",
journal = "ACS Omega",
title = "Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth",
number = "5",
volume = "4",
doi = "10.1021/acsomega.9b00512",
pages = "8094-8103"
}