TY  - CONF
AU  - AlJamal, Kamal
AU  - Stojadinović, Gordana
AU  - Živić, Miroslav
AU  - Todorović, Nataša
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4711
AB  - The chloride channel VRAC has been implicated in apoptosis, proliferation, transport of organic acids and regulatory volume decrease. Regulated by RAS/cGMP and by the ionic strength, it mediates efflux of anions (Cl-, glutamate, gluthatione, taurine, cGAMP). Knowing that chloride transport activity, mediated by NKCC, is indispensable for axon growth, we hypothesized that VRAC could have a role in neurite elongation mechanism. Experimental treatments compared to control were: VRAC selective inhibitor DCPIB (10 µM); BpV (phen) (80 nM), an inhibitor of PTEN. Axon growth was measured on cultured 
dissociated dorsal root ganglia neonatal neurons fixed 24h after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, total neurite length compared to control was decreased by BpV (p=0.0058) and to a lesser extent by DCPIB. In all measured parameters related to the degree of branching, BpV group neurons had small dispersion of values. In addition, DCPIB treated cells had 
larger fractal dimension (<0.05) than control neurons. The effect of DCPIB is mostly related to an increase in Fractal Dimension parameter, according to Principal Component Analysis. The increase in the number of primary branches per increment of cable length is significantly smaller in DCPIB group than in control and BpV -treated neurons. The DCPIB effect demonstrates that an anion current, presumably VRAC, has 
a role in the process of proper branching and directional elongation of neonatal DRG neurites.
C3  - EMBO Workshop Axons 2021: Structure and function
T1  - Morphometric comparison of neurite outgrowth in rat DRG cultures treated with DCPIB and BpV
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4711
ER  - 

@conference{
author = "AlJamal, Kamal and Stojadinović, Gordana and Živić, Miroslav and Todorović, Nataša",
year = "2021",
abstract = "The chloride channel VRAC has been implicated in apoptosis, proliferation, transport of organic acids and regulatory volume decrease. Regulated by RAS/cGMP and by the ionic strength, it mediates efflux of anions (Cl-, glutamate, gluthatione, taurine, cGAMP). Knowing that chloride transport activity, mediated by NKCC, is indispensable for axon growth, we hypothesized that VRAC could have a role in neurite elongation mechanism. Experimental treatments compared to control were: VRAC selective inhibitor DCPIB (10 µM); BpV (phen) (80 nM), an inhibitor of PTEN. Axon growth was measured on cultured 
dissociated dorsal root ganglia neonatal neurons fixed 24h after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, total neurite length compared to control was decreased by BpV (p=0.0058) and to a lesser extent by DCPIB. In all measured parameters related to the degree of branching, BpV group neurons had small dispersion of values. In addition, DCPIB treated cells had 
larger fractal dimension (<0.05) than control neurons. The effect of DCPIB is mostly related to an increase in Fractal Dimension parameter, according to Principal Component Analysis. The increase in the number of primary branches per increment of cable length is significantly smaller in DCPIB group than in control and BpV -treated neurons. The DCPIB effect demonstrates that an anion current, presumably VRAC, has 
a role in the process of proper branching and directional elongation of neonatal DRG neurites.",
journal = "EMBO Workshop Axons 2021: Structure and function",
title = "Morphometric comparison of neurite outgrowth in rat DRG cultures treated with DCPIB and BpV",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4711"
}

AlJamal, K., Stojadinović, G., Živić, M.,& Todorović, N.. (2021). Morphometric comparison of neurite outgrowth in rat DRG cultures treated with DCPIB and BpV. in EMBO Workshop Axons 2021: Structure and function, 74.
https://hdl.handle.net/21.15107/rcub_ibiss_4711

AlJamal K, Stojadinović G, Živić M, Todorović N. Morphometric comparison of neurite outgrowth in rat DRG cultures treated with DCPIB and BpV. in EMBO Workshop Axons 2021: Structure and function. 2021;:74.
https://hdl.handle.net/21.15107/rcub_ibiss_4711 .

AlJamal, Kamal, Stojadinović, Gordana, Živić, Miroslav, Todorović, Nataša, "Morphometric comparison of neurite outgrowth in rat DRG cultures treated with DCPIB and BpV" in EMBO Workshop Axons 2021: Structure and function (2021):74,
https://hdl.handle.net/21.15107/rcub_ibiss_4711 .

TY  - CONF
AU  - Todorović, Nataša
AU  - Stojadinović, Gordana
AU  - AlJamal, Kamal
AU  - Živić, Miroslav
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4678
AB  - Unlike the neurons in the CNS, the peripheral neurons have certain intrinsic regenerative capacity. 
After injury, peripheral neurons can switch to a cellular “state for growth”, with the expression profiles 
similar to early developmental stages. 
We looked at the changes of morphometric parameters induced in young peripheral neurons with 
treatments that in adult neurons have growth-stimulatory effect. The experimental treatments compared 
to control were: BpV (phen), an inhibitor of PTEN; and bFGF, basic fibroblast growth factor. The 
neurite growth was measured on cultured dissociated dorsal root ganglia neonatal neurons fixed 24h 
after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI 
Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, compared 
to control, total neurite length, length of primary and length of terminal branches, were increased by 
bFGF but not by BpV treatment. In all measured parameters related to the degree of branching, BpV treated neurons had small dispersion of values and small mean values, reminiscent of literature data 
stating that BpV treated neurons are elongated and less branched. However, the BpV did not have a 
positive influence on neurite elongation, as was reported on adult neurons. In contrast, bFGF stimulated 
elongation of young neurons in the manner similar to the effects described on the adult neurons.
PB  - Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac
C3  - 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
T1  - The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture
DO  - 10.46793/ICCBI21.214T
SP  - 214
EP  - 217
ER  - 

@conference{
author = "Todorović, Nataša and Stojadinović, Gordana and AlJamal, Kamal and Živić, Miroslav",
year = "2021",
abstract = "Unlike the neurons in the CNS, the peripheral neurons have certain intrinsic regenerative capacity. 
After injury, peripheral neurons can switch to a cellular “state for growth”, with the expression profiles 
similar to early developmental stages. 
We looked at the changes of morphometric parameters induced in young peripheral neurons with 
treatments that in adult neurons have growth-stimulatory effect. The experimental treatments compared 
to control were: BpV (phen), an inhibitor of PTEN; and bFGF, basic fibroblast growth factor. The 
neurite growth was measured on cultured dissociated dorsal root ganglia neonatal neurons fixed 24h 
after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI 
Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, compared 
to control, total neurite length, length of primary and length of terminal branches, were increased by 
bFGF but not by BpV treatment. In all measured parameters related to the degree of branching, BpV treated neurons had small dispersion of values and small mean values, reminiscent of literature data 
stating that BpV treated neurons are elongated and less branched. However, the BpV did not have a 
positive influence on neurite elongation, as was reported on adult neurons. In contrast, bFGF stimulated 
elongation of young neurons in the manner similar to the effects described on the adult neurons.",
publisher = "Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac",
journal = "1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia",
title = "The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture",
doi = "10.46793/ICCBI21.214T",
pages = "214-217"
}

Todorović, N., Stojadinović, G., AlJamal, K.,& Živić, M.. (2021). The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac., 214-217.
https://doi.org/10.46793/ICCBI21.214T

Todorović N, Stojadinović G, AlJamal K, Živić M. The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia. 2021;:214-217.
doi:10.46793/ICCBI21.214T .

Todorović, Nataša, Stojadinović, Gordana, AlJamal, Kamal, Živić, Miroslav, "The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture" in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia (2021):214-217,
https://doi.org/10.46793/ICCBI21.214T . .