TY  - CONF
AU  - Milošević, Katarina
AU  - Lavrnja, Irena
AU  - Janjić, Marija
AU  - Božić, Iva
AU  - Laketa, Danijela
AU  - Dacić, Sanja
AU  - Peković, Sanja
AU  - Savić, Danijela
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6004
AB  - Traumatic brain injury triggers neuroinflammatory response mediated by distinct populations of myeloid cells, including central nervous system (CNS) resident macrophages - microglia. Depending on the time upon insult this response may either contribute to restorative effects or hinder CNS repair.
Therefore, the focus of this study was on determining temporal course in gene expression profiles of markers specific to the mononuclear phagocyte system (MPS).
We have used the model of cortical stab injury which was performed on 3-months-old male Wistar rats. All animals were divided into 3 experimental groups: control, sham and lesion group and sacrificed at 1, 2, 3 and 7 days post-injury. After brain isolation, mRNA was extracted from cortical pieces around the center of lesion (the same tissue part was used for sham and control groups). The gene expression was analyzed by real-time PCR.
The mRNA levels of Itgam, Aif-1, Cd68 and Cx3Cr1, which are surface markers of MPS, were increased in first two days after brain injury, and then all, except Cd68, showed declining trend compared to control group. Furthermore, we analyzed expression of Arg-1, Il-6 and Tnf-alpha genes, which could be indicators of pro- or anti-inflammatory milieu. All of them increased significantly in the first two days post-injury, and then returned to control level, with the most prominent changes detected in Arg-1 mRNA level.
This study indicates enhanced MPS response in the acute phase after cortical stab injury. Further studies are required to determine which populations of CNS myeloid cells predominate in specific time point upon injury.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Mononuclear Phagocyte System In Traumatic Brain Injury
SP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6004
ER  - 

@conference{
author = "Milošević, Katarina and Lavrnja, Irena and Janjić, Marija and Božić, Iva and Laketa, Danijela and Dacić, Sanja and Peković, Sanja and Savić, Danijela",
year = "2019",
abstract = "Traumatic brain injury triggers neuroinflammatory response mediated by distinct populations of myeloid cells, including central nervous system (CNS) resident macrophages - microglia. Depending on the time upon insult this response may either contribute to restorative effects or hinder CNS repair.
Therefore, the focus of this study was on determining temporal course in gene expression profiles of markers specific to the mononuclear phagocyte system (MPS).
We have used the model of cortical stab injury which was performed on 3-months-old male Wistar rats. All animals were divided into 3 experimental groups: control, sham and lesion group and sacrificed at 1, 2, 3 and 7 days post-injury. After brain isolation, mRNA was extracted from cortical pieces around the center of lesion (the same tissue part was used for sham and control groups). The gene expression was analyzed by real-time PCR.
The mRNA levels of Itgam, Aif-1, Cd68 and Cx3Cr1, which are surface markers of MPS, were increased in first two days after brain injury, and then all, except Cd68, showed declining trend compared to control group. Furthermore, we analyzed expression of Arg-1, Il-6 and Tnf-alpha genes, which could be indicators of pro- or anti-inflammatory milieu. All of them increased significantly in the first two days post-injury, and then returned to control level, with the most prominent changes detected in Arg-1 mRNA level.
This study indicates enhanced MPS response in the acute phase after cortical stab injury. Further studies are required to determine which populations of CNS myeloid cells predominate in specific time point upon injury.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Mononuclear Phagocyte System In Traumatic Brain Injury",
pages = "503",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6004"
}

Milošević, K., Lavrnja, I., Janjić, M., Božić, I., Laketa, D., Dacić, S., Peković, S.,& Savić, D.. (2019). Mononuclear Phagocyte System In Traumatic Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 503.
https://hdl.handle.net/21.15107/rcub_ibiss_6004

Milošević K, Lavrnja I, Janjić M, Božić I, Laketa D, Dacić S, Peković S, Savić D. Mononuclear Phagocyte System In Traumatic Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:503.
https://hdl.handle.net/21.15107/rcub_ibiss_6004 .

Milošević, Katarina, Lavrnja, Irena, Janjić, Marija, Božić, Iva, Laketa, Danijela, Dacić, Sanja, Peković, Sanja, Savić, Danijela, "Mononuclear Phagocyte System In Traumatic Brain Injury" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):503,
https://hdl.handle.net/21.15107/rcub_ibiss_6004 .