TY  - JOUR
AU  - Milutinović, Violeta M.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Ušjak, Ljuboš J.
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana D.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200326
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5005
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
T2  - Chemistry & Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.
IS  - 7
VL  - 19
DO  - 10.1002/cbdv.202200326
SP  - e202200326
ER  - 

@article{
author = "Milutinović, Violeta M. and Matić, Ivana Z. and Stanojković, Tatjana P. and Soković, Marina and Ćirić, Ana and Ušjak, Ljuboš J. and Niketić, Marjan S. and Petrović, Silvana D.",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
journal = "Chemistry & Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.",
number = "7",
volume = "19",
doi = "10.1002/cbdv.202200326",
pages = "e202200326"
}

Milutinović, V. M., Matić, I. Z., Stanojković, T. P., Soković, M., Ćirić, A., Ušjak, L. J., Niketić, M. S.,& Petrović, S. D.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity, 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović VM, Matić IZ, Stanojković TP, Soković M, Ćirić A, Ušjak LJ, Niketić MS, Petrović SD. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta M., Matić, Ivana Z., Stanojković, Tatjana P., Soković, Marina, Ćirić, Ana, Ušjak, Ljuboš J., Niketić, Marjan S., Petrović, Silvana D., "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones." in Chemistry & Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - JOUR
AU  - Ušjak, Ljuboš J
AU  - Petrović, Silvana D
AU  - Drobac, Milica M
AU  - Soković, Marina
AU  - Stanojković, Tatjana P
AU  - Ćirić, Ana
AU  - Grozdanić, Nađa Ð
AU  - Niketić, Marjan S
PY  - 2016
UR  - http://onlinelibrary.wiley.com/doi/10.1002/cbdv.201500151/abstract
AB  - In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančić and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50 > 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50 = 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50 = 6.7 μg/ml).
PB  - Wiley-VCH Verlag
T2  - Chemistry & Biodiversity
T1  - Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.
IS  - 4
VL  - 13
DO  - 10.1002/cbdv.201500151
SP  - 466
EP  - 76
ER  - 

@article{
author = "Ušjak, Ljuboš J and Petrović, Silvana D and Drobac, Milica M and Soković, Marina and Stanojković, Tatjana P and Ćirić, Ana and Grozdanić, Nađa Ð and Niketić, Marjan S",
year = "2016",
abstract = "In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančić and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50 > 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50 = 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50 = 6.7 μg/ml).",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.",
number = "4",
volume = "13",
doi = "10.1002/cbdv.201500151",
pages = "466-76"
}

Ušjak, L. J., Petrović, S. D., Drobac, M. M., Soković, M., Stanojković, T. P., Ćirić, A., Grozdanić, N. Ð.,& Niketić, M. S.. (2016). Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.. in Chemistry & Biodiversity
Wiley-VCH Verlag., 13(4), 466-76.
https://doi.org/10.1002/cbdv.201500151

Ušjak LJ, Petrović SD, Drobac MM, Soković M, Stanojković TP, Ćirić A, Grozdanić NÐ, Niketić MS. Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils.. in Chemistry & Biodiversity. 2016;13(4):466-76.
doi:10.1002/cbdv.201500151 .

Ušjak, Ljuboš J, Petrović, Silvana D, Drobac, Milica M, Soković, Marina, Stanojković, Tatjana P, Ćirić, Ana, Grozdanić, Nađa Ð, Niketić, Marjan S, "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils." in Chemistry & Biodiversity, 13, no. 4 (2016):466-76,
https://doi.org/10.1002/cbdv.201500151 . .