TY  - JOUR
AU  - Milutinović, Violeta M.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Ušjak, Ljuboš J.
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana D.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200326
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5005
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
T2  - Chemistry & Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.
IS  - 7
VL  - 19
DO  - 10.1002/cbdv.202200326
SP  - e202200326
ER  - 

@article{
author = "Milutinović, Violeta M. and Matić, Ivana Z. and Stanojković, Tatjana P. and Soković, Marina and Ćirić, Ana and Ušjak, Ljuboš J. and Niketić, Marjan S. and Petrović, Silvana D.",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2 Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7-2.5 mg/mL MBC=3.4-5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15-0.4 mg/mL MFC=0.3-0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50 =148.1 μg/mL for SCH, and 152.3-303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50 =46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
journal = "Chemistry & Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.",
number = "7",
volume = "19",
doi = "10.1002/cbdv.202200326",
pages = "e202200326"
}

Milutinović, V. M., Matić, I. Z., Stanojković, T. P., Soković, M., Ćirić, A., Ušjak, L. J., Niketić, M. S.,& Petrović, S. D.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity, 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović VM, Matić IZ, Stanojković TP, Soković M, Ćirić A, Ušjak LJ, Niketić MS, Petrović SD. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones.. in Chemistry & Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta M., Matić, Ivana Z., Stanojković, Tatjana P., Soković, Marina, Ćirić, Ana, Ušjak, Ljuboš J., Niketić, Marjan S., Petrović, Silvana D., "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones." in Chemistry & Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - JOUR
AU  - Milošev, Milorad Z
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D
AU  - Stanojković, Tatjana
AU  - Matić, Ivana Z
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Kanazir, Selma
AU  - Mladenović, Milan
AU  - Rodić, Marko V
AU  - Leovac, Vukadin M
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2017
UR  - http://doi.wiley.com/10.1111/cbdd.12920
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27933733
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2753
AB  - A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.
T2  - Chemical Biology & Drug Design
T1  - Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies
IS  - 6
VL  - 89
DO  - 10.1111/cbdd.12920
SP  - 943
EP  - 952
ER  - 

@article{
author = "Milošev, Milorad Z and Jakovljević, Katarina and Joksović, Milan D and Stanojković, Tatjana and Matić, Ivana Z and Perović, Milka and Tešić, Vesna and Kanazir, Selma and Mladenović, Milan and Rodić, Marko V and Leovac, Vukadin M and Trifunović, Snežana and Marković, Violeta",
year = "2017",
abstract = "A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.",
journal = "Chemical Biology & Drug Design",
title = "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies",
number = "6",
volume = "89",
doi = "10.1111/cbdd.12920",
pages = "943-952"
}

Milošev, M. Z., Jakovljević, K., Joksović, M. D., Stanojković, T., Matić, I. Z., Perović, M., Tešić, V., Kanazir, S., Mladenović, M., Rodić, M. V., Leovac, V. M., Trifunović, S.,& Marković, V.. (2017). Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design, 89(6), 943-952.
https://doi.org/10.1111/cbdd.12920

Milošev MZ, Jakovljević K, Joksović MD, Stanojković T, Matić IZ, Perović M, Tešić V, Kanazir S, Mladenović M, Rodić MV, Leovac VM, Trifunović S, Marković V. Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design. 2017;89(6):943-952.
doi:10.1111/cbdd.12920 .

Milošev, Milorad Z, Jakovljević, Katarina, Joksović, Milan D, Stanojković, Tatjana, Matić, Ivana Z, Perović, Milka, Tešić, Vesna, Kanazir, Selma, Mladenović, Milan, Rodić, Marko V, Leovac, Vukadin M, Trifunović, Snežana, Marković, Violeta, "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies" in Chemical Biology & Drug Design, 89, no. 6 (2017):943-952,
https://doi.org/10.1111/cbdd.12920 . .