TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Gašić, Uroš
AU  - Žarković, Lazar D.
AU  - Krivošej, Zoran Đ.
AU  - Džamić, Ana M.
PY  - 2023
UR  - https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5345
AB  - The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
T2  - Records of Natural Products
T1  - Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
IS  - 3
VL  - 17
DO  - 10.25135/rnp.364.2207.2528
SP  - 453
EP  - 475
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Gašić, Uroš and Žarković, Lazar D. and Krivošej, Zoran Đ. and Džamić, Ana M.",
year = "2023",
abstract = "The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.",
journal = "Records of Natural Products",
title = "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia",
number = "3",
volume = "17",
doi = "10.25135/rnp.364.2207.2528",
pages = "453-475"
}

Mileski, K. S., Ćirić, A., Gašić, U., Žarković, L. D., Krivošej, Z. Đ.,& Džamić, A. M.. (2023). Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products, 17(3), 453-475.
https://doi.org/10.25135/rnp.364.2207.2528

Mileski KS, Ćirić A, Gašić U, Žarković LD, Krivošej ZĐ, Džamić AM. Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products. 2023;17(3):453-475.
doi:10.25135/rnp.364.2207.2528 .

Mileski, Ksenija S., Ćirić, Ana, Gašić, Uroš, Žarković, Lazar D., Krivošej, Zoran Đ., Džamić, Ana M., "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia" in Records of Natural Products, 17, no. 3 (2023):453-475,
https://doi.org/10.25135/rnp.364.2207.2528 . .