TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Đorović Jovanović, Jelena
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Nikles, Stefanie
AU  - Marković, Zoran
AU  - Bauer, Rudolf
PY  - 2023
UR  - https://www.mdpi.com/1420-3049/28/3/1274
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9921532
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5482
AB  - Rabelera holostea (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as Stellaria holostea L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of R. holostea aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and p-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of R. holostea extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as F. oxysporum (MIC 1.25 mg/mL), whereas the capacity of R. holostea to affect the growth of bacteria was much less pronounced. R. holostea extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031274
SP  - 1274
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Đorović Jovanović, Jelena and Mišić, Danijela and Gašić, Uroš and Nikles, Stefanie and Marković, Zoran and Bauer, Rudolf",
year = "2023",
abstract = "Rabelera holostea (L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as Stellaria holostea L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of R. holostea aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and p-coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of R. holostea extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as F. oxysporum (MIC 1.25 mg/mL), whereas the capacity of R. holostea to affect the growth of bacteria was much less pronounced. R. holostea extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract",
number = "3",
volume = "28",
doi = "10.3390/molecules28031274",
pages = "1274"
}

Katanić Stanković, J. S., Đorović Jovanović, J., Mišić, D., Gašić, U., Nikles, S., Marković, Z.,& Bauer, R.. (2023). UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract. in Molecules (Basel, Switzerland)
Basel: MDPI., 28(3), 1274.
https://doi.org/10.3390/molecules28031274

Katanić Stanković JS, Đorović Jovanović J, Mišić D, Gašić U, Nikles S, Marković Z, Bauer R. UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract. in Molecules (Basel, Switzerland). 2023;28(3):1274.
doi:10.3390/molecules28031274 .

Katanić Stanković, Jelena S., Đorović Jovanović, Jelena, Mišić, Danijela, Gašić, Uroš, Nikles, Stefanie, Marković, Zoran, Bauer, Rudolf, "UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract" in Molecules (Basel, Switzerland), 28, no. 3 (2023):1274,
https://doi.org/10.3390/molecules28031274 . .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Smederevac-Lalić, Marija
AU  - Marković, Zoran
AU  - Skorić, Stefan
AU  - Jaćimović, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5723
AB  - Invasive alien fish species represent one of the greatest threats to the
biodiversity and ecological status of freshwater ecosystems. Within their
management, their application as bioindicators of pollution in monitoring
programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish
in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay.
Sampling was performed from June to September 2021 on Markovačko
Lake, which is exposed to diffuse pollution from the application of pesticides
on the surrounding agricultural land. Monitoring also included the
assessment of water quality by basic physical and chemical parameters, as
well as microbiological indicators of faecal pollution. Alkaline comet assay
was performed on fish erythrocytes, on ten specimens per month. The study
also included ten control fish from the recirculating aquaculture system
(RAS), which were transferred from Markovačko Lake for breeding, and
treatment of their erythrocytes with H2O2 as a positive control. Scoring of
comets was performed by CometScore 2.0 software. The lowest level of
DNA damage was recorded in the control group and the highest in the
positive control group. August was the month with the lowest level of DNA
damage, and September with the highest. Statistically significant
differences were found between all groups indicating a high sensitivity of
the alkaline comet assay to discriminate fine differences in the genotoxic
potential of water. Knowing that black bullhead is highly abundant in
stagnant waters and relatively easy to sample, this study draws additional
attention to its use as a bioindicator of genotoxicity.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Smederevac-Lalić, Marija and Marković, Zoran and Skorić, Stefan and Jaćimović, Milica",
year = "2022",
abstract = "Invasive alien fish species represent one of the greatest threats to the
biodiversity and ecological status of freshwater ecosystems. Within their
management, their application as bioindicators of pollution in monitoring
programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish
in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay.
Sampling was performed from June to September 2021 on Markovačko
Lake, which is exposed to diffuse pollution from the application of pesticides
on the surrounding agricultural land. Monitoring also included the
assessment of water quality by basic physical and chemical parameters, as
well as microbiological indicators of faecal pollution. Alkaline comet assay
was performed on fish erythrocytes, on ten specimens per month. The study
also included ten control fish from the recirculating aquaculture system
(RAS), which were transferred from Markovačko Lake for breeding, and
treatment of their erythrocytes with H2O2 as a positive control. Scoring of
comets was performed by CometScore 2.0 software. The lowest level of
DNA damage was recorded in the control group and the highest in the
positive control group. August was the month with the lowest level of DNA
damage, and September with the highest. Statistically significant
differences were found between all groups indicating a high sensitivity of
the alkaline comet assay to discriminate fine differences in the genotoxic
potential of water. Knowing that black bullhead is highly abundant in
stagnant waters and relatively easy to sample, this study draws additional
attention to its use as a bioindicator of genotoxicity.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5723"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Smederevac-Lalić, M., Marković, Z., Skorić, S.,& Jaćimović, M.. (2022). Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5723

Kostić-Vuković J, Sunjog K, Kolarević S, Smederevac-Lalić M, Marković Z, Skorić S, Jaćimović M. Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5723 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Smederevac-Lalić, Marija, Marković, Zoran, Skorić, Stefan, Jaćimović, Milica, "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5723 .

TY  - JOUR
AU  - Vranković, Jelena
AU  - Božanić, Milenka
AU  - Živić, Miroslav
AU  - Marković, Zoran
AU  - Marjanović, Stefan
AU  - Golubović, Vukosav
AU  - Živić, Ivana
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2352513422003830
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5176
AB  - A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna.
PB  - Elsevier B.V.
T2  - Aquaculture Reports
T1  - Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns
VL  - 27
DO  - 10.1016/j.aqrep.2022.101387
SP  - 101387
ER  - 

@article{
author = "Vranković, Jelena and Božanić, Milenka and Živić, Miroslav and Marković, Zoran and Marjanović, Stefan and Golubović, Vukosav and Živić, Ivana",
year = "2022",
abstract = "A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna.",
publisher = "Elsevier B.V.",
journal = "Aquaculture Reports",
title = "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns",
volume = "27",
doi = "10.1016/j.aqrep.2022.101387",
pages = "101387"
}

Vranković, J., Božanić, M., Živić, M., Marković, Z., Marjanović, S., Golubović, V.,& Živić, I.. (2022). Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports
Elsevier B.V.., 27, 101387.
https://doi.org/10.1016/j.aqrep.2022.101387

Vranković J, Božanić M, Živić M, Marković Z, Marjanović S, Golubović V, Živić I. Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports. 2022;27:101387.
doi:10.1016/j.aqrep.2022.101387 .

Vranković, Jelena, Božanić, Milenka, Živić, Miroslav, Marković, Zoran, Marjanović, Stefan, Golubović, Vukosav, Živić, Ivana, "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns" in Aquaculture Reports, 27 (2022):101387,
https://doi.org/10.1016/j.aqrep.2022.101387 . .

TY  - JOUR
AU  - Božanić, Milenka L.J.
AU  - Todorović, Dajana
AU  - Živić, Miroslav Ž.
AU  - Perić Mataruga, Vesna
AU  - Marković, Zoran Z.
AU  - Živić, Ivana M.
PY  - 2018
UR  - https://www.kmae-journal.org/10.1051/kmae/2018036
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3211
AB  - The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1–control, and SK2, SK3, and SK4–30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapež River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3−), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3−. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.
PB  - EDP Sciences
T2  - Knowledge and Management of Aquatic Ecosystems
T1  - Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)
IS  - 419
DO  - 10.1051/kmae/2018036
SP  - 47
ER  - 

@article{
author = "Božanić, Milenka L.J. and Todorović, Dajana and Živić, Miroslav Ž. and Perić Mataruga, Vesna and Marković, Zoran Z. and Živić, Ivana M.",
year = "2018",
abstract = "The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1–control, and SK2, SK3, and SK4–30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapež River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3−), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3−. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.",
publisher = "EDP Sciences",
journal = "Knowledge and Management of Aquatic Ecosystems",
title = "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)",
number = "419",
doi = "10.1051/kmae/2018036",
pages = "47"
}

Božanić, M. L.J., Todorović, D., Živić, M. Ž., Perić Mataruga, V., Marković, Z. Z.,& Živić, I. M.. (2018). Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems
EDP Sciences.(419), 47.
https://doi.org/10.1051/kmae/2018036

Božanić ML, Todorović D, Živić MŽ, Perić Mataruga V, Marković ZZ, Živić IM. Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems. 2018;(419):47.
doi:10.1051/kmae/2018036 .

Božanić, Milenka L.J., Todorović, Dajana, Živić, Miroslav Ž., Perić Mataruga, Vesna, Marković, Zoran Z., Živić, Ivana M., "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)" in Knowledge and Management of Aquatic Ecosystems, no. 419 (2018):47,
https://doi.org/10.1051/kmae/2018036 . .

TY  - JOUR
AU  - Isaković, Aleksandra
AU  - Marković, Zoran
AU  - Todorović-Marković, Biljana
AU  - Nikolić, Nadežda
AU  - Vranješ-Đurić, Sanja
AU  - Mirković, Marija
AU  - Dramićanin, Miroslav
AU  - Harhaji-Trajković, Ljubica
AU  - Zogović, Nevena
AU  - Nikolić, Zoran
AU  - Trajković, Vladimir
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3589
AB  - The mechanisms underlying the cytotoxic action of pure fullerene suspension (nano-C 60 ) and water-soluble polyhydroxylated fullerene [C 60 (OH) n ] were investigated. Crystal violet assay for cell viability demonstrated that nano-C 60 was at least three orders of magnitude more toxic than C 60 (OH) n to mouse L929 fibrosarcoma, rat C6 glioma, and U251 human glioma cell lines. Flow cytometry analysis of cells stained with propidium iodide (PI), PI/annexin V-fluorescein isothiocyanate, or the redox-sensitive dye dihydrorhodamine revealed that nano-C 60 caused rapid (observable after few hours), reactive oxygen species (ROS)-associated necrosis characterized by cell membrane damage without DNA fragmentation. In contrast, C 60 (OH) n caused delayed, ROS-independent cell death with characteristics of apoptosis, including DNA fragmentation and loss of cell membrane asymmetry in the absence of increased permeability. Accordingly, the antioxidant N-acetylcysteine protected the cell lines from nano-C 60 toxicity, but not C 60 (OH) n toxicity, while the pan-caspase inhibitor z-VAD-fmk blocked C 60 (OH) n -induced apoptosis, but not nano-C 60 -mediated necrosis. Finally, C 60 (OH) n antagomozed, while nano-C 60 synergized with, the cytotoxic action of oxidative stress-inducing agents hydrogen peroxide and peroxynitrite donor 3-morpholinosydnonimine. Therefore, unlike polyhydroxylated C 60 that exerts mainly antioxidant/cytoprotective and only mild ROS-independent pro-apoptotic activity, pure crystalline C 60 seems to be endowed with strong pro-oxidant capacity responsible for the rapid necrotic cell death.
PB  - Oxford University Press (OUP)
T2  - Toxicological Sciences
T1  - Distinct Cytotoxic Mechanisms of Pristine versus Hydroxylated Fullerene
IS  - 1
VL  - 91
DO  - 10.1093/toxsci/kfj127
SP  - 173
EP  - 183
ER  - 

@article{
author = "Isaković, Aleksandra and Marković, Zoran and Todorović-Marković, Biljana and Nikolić, Nadežda and Vranješ-Đurić, Sanja and Mirković, Marija and Dramićanin, Miroslav and Harhaji-Trajković, Ljubica and Zogović, Nevena and Nikolić, Zoran and Trajković, Vladimir",
year = "2006",
abstract = "The mechanisms underlying the cytotoxic action of pure fullerene suspension (nano-C 60 ) and water-soluble polyhydroxylated fullerene [C 60 (OH) n ] were investigated. Crystal violet assay for cell viability demonstrated that nano-C 60 was at least three orders of magnitude more toxic than C 60 (OH) n to mouse L929 fibrosarcoma, rat C6 glioma, and U251 human glioma cell lines. Flow cytometry analysis of cells stained with propidium iodide (PI), PI/annexin V-fluorescein isothiocyanate, or the redox-sensitive dye dihydrorhodamine revealed that nano-C 60 caused rapid (observable after few hours), reactive oxygen species (ROS)-associated necrosis characterized by cell membrane damage without DNA fragmentation. In contrast, C 60 (OH) n caused delayed, ROS-independent cell death with characteristics of apoptosis, including DNA fragmentation and loss of cell membrane asymmetry in the absence of increased permeability. Accordingly, the antioxidant N-acetylcysteine protected the cell lines from nano-C 60 toxicity, but not C 60 (OH) n toxicity, while the pan-caspase inhibitor z-VAD-fmk blocked C 60 (OH) n -induced apoptosis, but not nano-C 60 -mediated necrosis. Finally, C 60 (OH) n antagomozed, while nano-C 60 synergized with, the cytotoxic action of oxidative stress-inducing agents hydrogen peroxide and peroxynitrite donor 3-morpholinosydnonimine. Therefore, unlike polyhydroxylated C 60 that exerts mainly antioxidant/cytoprotective and only mild ROS-independent pro-apoptotic activity, pure crystalline C 60 seems to be endowed with strong pro-oxidant capacity responsible for the rapid necrotic cell death.",
publisher = "Oxford University Press (OUP)",
journal = "Toxicological Sciences",
title = "Distinct Cytotoxic Mechanisms of Pristine versus Hydroxylated Fullerene",
number = "1",
volume = "91",
doi = "10.1093/toxsci/kfj127",
pages = "173-183"
}

Isaković, A., Marković, Z., Todorović-Marković, B., Nikolić, N., Vranješ-Đurić, S., Mirković, M., Dramićanin, M., Harhaji-Trajković, L., Zogović, N., Nikolić, Z.,& Trajković, V.. (2006). Distinct Cytotoxic Mechanisms of Pristine versus Hydroxylated Fullerene. in Toxicological Sciences
Oxford University Press (OUP)., 91(1), 173-183.
https://doi.org/10.1093/toxsci/kfj127

Isaković A, Marković Z, Todorović-Marković B, Nikolić N, Vranješ-Đurić S, Mirković M, Dramićanin M, Harhaji-Trajković L, Zogović N, Nikolić Z, Trajković V. Distinct Cytotoxic Mechanisms of Pristine versus Hydroxylated Fullerene. in Toxicological Sciences. 2006;91(1):173-183.
doi:10.1093/toxsci/kfj127 .

Isaković, Aleksandra, Marković, Zoran, Todorović-Marković, Biljana, Nikolić, Nadežda, Vranješ-Đurić, Sanja, Mirković, Marija, Dramićanin, Miroslav, Harhaji-Trajković, Ljubica, Zogović, Nevena, Nikolić, Zoran, Trajković, Vladimir, "Distinct Cytotoxic Mechanisms of Pristine versus Hydroxylated Fullerene" in Toxicological Sciences, 91, no. 1 (2006):173-183,
https://doi.org/10.1093/toxsci/kfj127 . .