TY  - JOUR
AU  - Tepavcevic, Snezana
AU  - Vojnović-Milutinović, Danijela
AU  - Macut, Djuro
AU  - Zakula, Zorica
AU  - Radovanović, Marina
AU  - Bozic-Antic, Ivana
AU  - Romic, Snjezana
AU  - Bjekić-Macut, Jelica
AU  - Matić, Gordana
AU  - Koricanac, Goran
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2217
AB  - It is supposed that women with polycystic ovary syndrome (PCOS) are
   prone to develop cardiovascular disease as a consequence of multiple
   risk factors that are mostly related to the state of insulin resistance
   and consequent hyperinsulinemia. In the present study, we evaluated
   insulin signaling and glucose transporters (GLUT) in cardiac cells of
   dihydrotestosterone (DHT) treated female rats as an animal model of
   PCOS. Expression of proteins involved in cardiac insulin signaling
   pathways and glucose transporters, as well as their phosphorylation or
   intracellular localization were studied by Western blot analysis in
   DHT-treated and control rats. Treatment with DHT resulted in increased
   body mass, absolute mass of the heart, elevated plasma insulin
   concentration, dyslipidemia and insulin resistance. At the molecular
   level, DHT treatment did not change protein expression of cardiac
   insulin receptor and insulin receptor substrate 1, while phosphorylation
   of the substrate at serine 307 was increased. Unexpectedly, although
   expression of downstream Akt kinase and its phosphorylation at threonine
   308 were not altered, phosphoiylation of Akt at serine 473 was increased
   in the heart of DHT-treated rats. In contrast, expression and
   phosphorylation of extracellular signal regulated kinases 1/2 were
   decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased,
   as well as the expression of GLUT4 in cardiac cells at the end of
   androgen treatment. The obtained results provide evidence for
   alterations in expression and especially in functional characteristics
   of insulin signaling molecules and glucose transporters in the heart of
   DHT-treated rats with PCOS, indicating impaired cardiac insulin action.
   (c) 2014 Elsevier Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome
VL  - 141
DO  - 10.1016/j.jsbmb.2014.01.006
SP  - 71
EP  - 76
ER  - 

@article{
author = "Tepavcevic, Snezana and Vojnović-Milutinović, Danijela and Macut, Djuro and Zakula, Zorica and Radovanović, Marina and Bozic-Antic, Ivana and Romic, Snjezana and Bjekić-Macut, Jelica and Matić, Gordana and Koricanac, Goran",
year = "2014",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are
   prone to develop cardiovascular disease as a consequence of multiple
   risk factors that are mostly related to the state of insulin resistance
   and consequent hyperinsulinemia. In the present study, we evaluated
   insulin signaling and glucose transporters (GLUT) in cardiac cells of
   dihydrotestosterone (DHT) treated female rats as an animal model of
   PCOS. Expression of proteins involved in cardiac insulin signaling
   pathways and glucose transporters, as well as their phosphorylation or
   intracellular localization were studied by Western blot analysis in
   DHT-treated and control rats. Treatment with DHT resulted in increased
   body mass, absolute mass of the heart, elevated plasma insulin
   concentration, dyslipidemia and insulin resistance. At the molecular
   level, DHT treatment did not change protein expression of cardiac
   insulin receptor and insulin receptor substrate 1, while phosphorylation
   of the substrate at serine 307 was increased. Unexpectedly, although
   expression of downstream Akt kinase and its phosphorylation at threonine
   308 were not altered, phosphoiylation of Akt at serine 473 was increased
   in the heart of DHT-treated rats. In contrast, expression and
   phosphorylation of extracellular signal regulated kinases 1/2 were
   decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased,
   as well as the expression of GLUT4 in cardiac cells at the end of
   androgen treatment. The obtained results provide evidence for
   alterations in expression and especially in functional characteristics
   of insulin signaling molecules and glucose transporters in the heart of
   DHT-treated rats with PCOS, indicating impaired cardiac insulin action.
   (c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome",
volume = "141",
doi = "10.1016/j.jsbmb.2014.01.006",
pages = "71-76"
}

Tepavcevic, S., Vojnović-Milutinović, D., Macut, D., Zakula, Z., Radovanović, M., Bozic-Antic, I., Romic, S., Bjekić-Macut, J., Matić, G.,& Koricanac, G.. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76.
https://doi.org/10.1016/j.jsbmb.2014.01.006

Tepavcevic S, Vojnović-Milutinović D, Macut D, Zakula Z, Radovanović M, Bozic-Antic I, Romic S, Bjekić-Macut J, Matić G, Koricanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76.
doi:10.1016/j.jsbmb.2014.01.006 .

Tepavcevic, Snezana, Vojnović-Milutinović, Danijela, Macut, Djuro, Zakula, Zorica, Radovanović, Marina, Bozic-Antic, Ivana, Romic, Snjezana, Bjekić-Macut, Jelica, Matić, Gordana, Koricanac, Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome" in Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76,
https://doi.org/10.1016/j.jsbmb.2014.01.006 . .