TY  - JOUR
AU  - Vujičić, Milica
AU  - Vasić, Bobana
AU  - Nikolić, Ivana
AU  - Saksida, Tamara
AU  - Stojanović, Ivana D.
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600096V
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2978
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/812
AB  - Nuclear protein 1 (NUPR1) is a transcription cofactor that senses stressful conditions and modulates cellular response by promoting or inhibiting apoptosis. NUPR1 is usually highly expressed in tumor cells where it enables them to adapt and resist environmental stress or chemotherapeutic compounds. NUPR1 can be involved in cell proliferation. Data about the involvement of NUPR1 in the proliferation and apoptosis of lymphocytes are scarce. Therefore, in this study we focused on the role of NUPR1 in lymphocyte physiology and found that NUPR1 might be involved in the initiation of their proliferation. Lymphocytes were isolated from the cervical lymph nodes of C57BL/6 mice. NUPR1 expression subsided 24 h after the induction of proliferation by a mitogen. Also, stressful conditions after cell isolation led to increased NUPR1 mRNA and protein expression in vitro that coincided with cell apoptosis. Similarly, apoptosis induction by staurosporine, a broad-range protein kinase inhibitor, led to increased NUPR1 expression. In addition, NUPR1 inhibition by smallinterfering RNA prevented the staurosporine-induced apoptosis (judging from decreased caspase activity) in the whole cell population of cervical lymph nodes. However, NUPR1 absence was irrelevant to the induction of apoptosis in CD3+ T lymphocytes, suggesting that NUPR1 is probably a mediator of apoptosis in other immune cell populations within the lymph node, such as B lymphocytes. In conclusion, our results suggest that NUPR1 is important for the initiation of lymphocyte cell division and for the apoptotic process of non-T cells during stressful conditions.
T2  - Archives of Biological Sciences
T1  - The role of NUPR1 in lymphocyte proliferation and apoptosis
IS  - 2
VL  - 69
DO  - 10.2298/ABS160707096V
SP  - 261
EP  - 267
ER  - 

@article{
author = "Vujičić, Milica and Vasić, Bobana and Nikolić, Ivana and Saksida, Tamara and Stojanović, Ivana D.",
year = "2017",
abstract = "Nuclear protein 1 (NUPR1) is a transcription cofactor that senses stressful conditions and modulates cellular response by promoting or inhibiting apoptosis. NUPR1 is usually highly expressed in tumor cells where it enables them to adapt and resist environmental stress or chemotherapeutic compounds. NUPR1 can be involved in cell proliferation. Data about the involvement of NUPR1 in the proliferation and apoptosis of lymphocytes are scarce. Therefore, in this study we focused on the role of NUPR1 in lymphocyte physiology and found that NUPR1 might be involved in the initiation of their proliferation. Lymphocytes were isolated from the cervical lymph nodes of C57BL/6 mice. NUPR1 expression subsided 24 h after the induction of proliferation by a mitogen. Also, stressful conditions after cell isolation led to increased NUPR1 mRNA and protein expression in vitro that coincided with cell apoptosis. Similarly, apoptosis induction by staurosporine, a broad-range protein kinase inhibitor, led to increased NUPR1 expression. In addition, NUPR1 inhibition by smallinterfering RNA prevented the staurosporine-induced apoptosis (judging from decreased caspase activity) in the whole cell population of cervical lymph nodes. However, NUPR1 absence was irrelevant to the induction of apoptosis in CD3+ T lymphocytes, suggesting that NUPR1 is probably a mediator of apoptosis in other immune cell populations within the lymph node, such as B lymphocytes. In conclusion, our results suggest that NUPR1 is important for the initiation of lymphocyte cell division and for the apoptotic process of non-T cells during stressful conditions.",
journal = "Archives of Biological Sciences",
title = "The role of NUPR1 in lymphocyte proliferation and apoptosis",
number = "2",
volume = "69",
doi = "10.2298/ABS160707096V",
pages = "261-267"
}

Vujičić, M., Vasić, B., Nikolić, I., Saksida, T.,& Stojanović, I. D.. (2017). The role of NUPR1 in lymphocyte proliferation and apoptosis. in Archives of Biological Sciences, 69(2), 261-267.
https://doi.org/10.2298/ABS160707096V

Vujičić M, Vasić B, Nikolić I, Saksida T, Stojanović ID. The role of NUPR1 in lymphocyte proliferation and apoptosis. in Archives of Biological Sciences. 2017;69(2):261-267.
doi:10.2298/ABS160707096V .

Vujičić, Milica, Vasić, Bobana, Nikolić, Ivana, Saksida, Tamara, Stojanović, Ivana D., "The role of NUPR1 in lymphocyte proliferation and apoptosis" in Archives of Biological Sciences, 69, no. 2 (2017):261-267,
https://doi.org/10.2298/ABS160707096V . .

TY  - JOUR
AU  - Vujičić, Milica
AU  - Vasić, Bobana
AU  - Nikolić, Ivana
AU  - Saksida, Tamara
AU  - Stojanović, Ivana D.
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600096V
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2690
AB  - Nuclear protein 1 (NUPR1) is a transcription cofactor that senses stressful conditions and modulates cellular response by promoting or inhibiting apoptosis. NUPR1 is usually highly expressed in tumor cells where it enables them to adapt and resist environmental stress or chemotherapeutic compounds. NUPR1 can be involved in cell proliferation. Data about the involvement of NUPR1 in the proliferation and apoptosis of lymphocytes are scarce. Therefore, in this study we focused on the role of NUPR1 in lymphocyte physiology and found that NUPR1 might be involved in the initiation of their proliferation. Lymphocytes were isolated from the cervical lymph nodes of C57BL/6 mice. NUPR1 expression subsided 24 h after the induction of proliferation by a mitogen. Also, stressful conditions after cell isolation led to increased NUPR1 mRNA and protein expression in vitro that coincided with cell apoptosis. Similarly, apoptosis induction by staurosporine, a broad-range protein kinase inhibitor, led to increased NUPR1 expression. In addition, NUPR1 inhibition by smallinterfering RNA prevented the staurosporine-induced apoptosis (judging from decreased caspase activity) in the whole cell population of cervical lymph nodes. However, NUPR1 absence was irrelevant to the induction of apoptosis in CD3+ T lymphocytes, suggesting that NUPR1 is probably a mediator of apoptosis in other immune cell populations within the lymph node, such as B lymphocytes. In conclusion, our results suggest that NUPR1 is important for the initiation of lymphocyte cell division and for the apoptotic process of non-T cells during stressful conditions.
T2  - Archives of Biological Sciences
T1  - The role of NUPR1 in lymphocyte proliferation and apoptosis
DO  - 10.2298/ABS160707096V
ER  - 

@article{
author = "Vujičić, Milica and Vasić, Bobana and Nikolić, Ivana and Saksida, Tamara and Stojanović, Ivana D.",
year = "2016",
abstract = "Nuclear protein 1 (NUPR1) is a transcription cofactor that senses stressful conditions and modulates cellular response by promoting or inhibiting apoptosis. NUPR1 is usually highly expressed in tumor cells where it enables them to adapt and resist environmental stress or chemotherapeutic compounds. NUPR1 can be involved in cell proliferation. Data about the involvement of NUPR1 in the proliferation and apoptosis of lymphocytes are scarce. Therefore, in this study we focused on the role of NUPR1 in lymphocyte physiology and found that NUPR1 might be involved in the initiation of their proliferation. Lymphocytes were isolated from the cervical lymph nodes of C57BL/6 mice. NUPR1 expression subsided 24 h after the induction of proliferation by a mitogen. Also, stressful conditions after cell isolation led to increased NUPR1 mRNA and protein expression in vitro that coincided with cell apoptosis. Similarly, apoptosis induction by staurosporine, a broad-range protein kinase inhibitor, led to increased NUPR1 expression. In addition, NUPR1 inhibition by smallinterfering RNA prevented the staurosporine-induced apoptosis (judging from decreased caspase activity) in the whole cell population of cervical lymph nodes. However, NUPR1 absence was irrelevant to the induction of apoptosis in CD3+ T lymphocytes, suggesting that NUPR1 is probably a mediator of apoptosis in other immune cell populations within the lymph node, such as B lymphocytes. In conclusion, our results suggest that NUPR1 is important for the initiation of lymphocyte cell division and for the apoptotic process of non-T cells during stressful conditions.",
journal = "Archives of Biological Sciences",
title = "The role of NUPR1 in lymphocyte proliferation and apoptosis",
doi = "10.2298/ABS160707096V"
}

Vujičić, M., Vasić, B., Nikolić, I., Saksida, T.,& Stojanović, I. D.. (2016). The role of NUPR1 in lymphocyte proliferation and apoptosis. in Archives of Biological Sciences.
https://doi.org/10.2298/ABS160707096V

Vujičić M, Vasić B, Nikolić I, Saksida T, Stojanović ID. The role of NUPR1 in lymphocyte proliferation and apoptosis. in Archives of Biological Sciences. 2016;.
doi:10.2298/ABS160707096V .

Vujičić, Milica, Vasić, Bobana, Nikolić, Ivana, Saksida, Tamara, Stojanović, Ivana D., "The role of NUPR1 in lymphocyte proliferation and apoptosis" in Archives of Biological Sciences (2016),
https://doi.org/10.2298/ABS160707096V . .

TY  - JOUR
AU  - Vujičić, Milica
AU  - Nikolić, Ivana
AU  - Kontogianni, Vassiliki G.
AU  - Saksida, Tamara
AU  - Charisiadis, Pantelis
AU  - Vasić, Bobana
AU  - Stošić-Grujičić, Stanislava
AU  - Gerothanassis, Ioannis P.
AU  - Tzakos, Andreas G.
AU  - Stojanović, Ivana D.
PY  - 2016
UR  - http://doi.wiley.com/10.1111/1750-3841.13333
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2979
AB  - Type 1 diabetes (T1D) is an autoimmune disease that develops as a consequence of pancreatic β-cell death induced by proinflammatory mediators. Because Origanum vulgare L. ssp. hirtum (Greek oregano) contains antiinflammatory molecules, we hypothesized that it might be beneficial for the treatment of T1D. An ethyl acetate extract of oregano (EAO) was prepared from the leaves by a polar extraction method. Phytochemical composition was determined by liquid chromatography-UV diode array coupled to ion-trap mass spectrometry with electrospray ionization interface (LC/DAD/ESI-MSn). In vitro immunomodulatory effect of EAO was estimated by measuring proliferation (MTT) or cytokine secretion (ELISA) from immune cells. Diabetes was induced by multiple low doses of streptozotocin (MLDS) in male C57BL/6 mice and EAO was administered intraperitoneally for 10 d. Determination of cellular composition (flow cytometry) and cytokine production (ELISA) was performed on 12th d after diabetes induction. EAO suppressed the function of both macrophages and lymphocytes in vitro. In vivo, EAO treatment significantly preserved pancreatic islets and reduced diabetes incidence in MLDS-challenged mice. Besides down-modulatory effect on macrophages, EAO reduced the number of total CD4+ and activated CD4+CD25+ T cells. Furthermore, EAO affected the number of T helper 1 (Th1) and T helper 17 (Th17) cells through downregulation of their key transcription factors T-bet and RORγT. Because EAO treatment protects mice from development of hyperglycemia by reducing proinflammatory macrophage/Th1/Th17 response, this plant extract could represent a basis for future diabetes therapy.
PB  - Blackwell Publishing Inc.
T2  - Journal of Food Science
T1  - Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice
IS  - 7
VL  - 81
DO  - 10.1111/1750-3841.13333
SP  - H1846
EP  - H1853
ER  - 

@article{
author = "Vujičić, Milica and Nikolić, Ivana and Kontogianni, Vassiliki G. and Saksida, Tamara and Charisiadis, Pantelis and Vasić, Bobana and Stošić-Grujičić, Stanislava and Gerothanassis, Ioannis P. and Tzakos, Andreas G. and Stojanović, Ivana D.",
year = "2016",
abstract = "Type 1 diabetes (T1D) is an autoimmune disease that develops as a consequence of pancreatic β-cell death induced by proinflammatory mediators. Because Origanum vulgare L. ssp. hirtum (Greek oregano) contains antiinflammatory molecules, we hypothesized that it might be beneficial for the treatment of T1D. An ethyl acetate extract of oregano (EAO) was prepared from the leaves by a polar extraction method. Phytochemical composition was determined by liquid chromatography-UV diode array coupled to ion-trap mass spectrometry with electrospray ionization interface (LC/DAD/ESI-MSn). In vitro immunomodulatory effect of EAO was estimated by measuring proliferation (MTT) or cytokine secretion (ELISA) from immune cells. Diabetes was induced by multiple low doses of streptozotocin (MLDS) in male C57BL/6 mice and EAO was administered intraperitoneally for 10 d. Determination of cellular composition (flow cytometry) and cytokine production (ELISA) was performed on 12th d after diabetes induction. EAO suppressed the function of both macrophages and lymphocytes in vitro. In vivo, EAO treatment significantly preserved pancreatic islets and reduced diabetes incidence in MLDS-challenged mice. Besides down-modulatory effect on macrophages, EAO reduced the number of total CD4+ and activated CD4+CD25+ T cells. Furthermore, EAO affected the number of T helper 1 (Th1) and T helper 17 (Th17) cells through downregulation of their key transcription factors T-bet and RORγT. Because EAO treatment protects mice from development of hyperglycemia by reducing proinflammatory macrophage/Th1/Th17 response, this plant extract could represent a basis for future diabetes therapy.",
publisher = "Blackwell Publishing Inc.",
journal = "Journal of Food Science",
title = "Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice",
number = "7",
volume = "81",
doi = "10.1111/1750-3841.13333",
pages = "H1846-H1853"
}

Vujičić, M., Nikolić, I., Kontogianni, V. G., Saksida, T., Charisiadis, P., Vasić, B., Stošić-Grujičić, S., Gerothanassis, I. P., Tzakos, A. G.,& Stojanović, I. D.. (2016). Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice. in Journal of Food Science
Blackwell Publishing Inc.., 81(7), H1846-H1853.
https://doi.org/10.1111/1750-3841.13333

Vujičić M, Nikolić I, Kontogianni VG, Saksida T, Charisiadis P, Vasić B, Stošić-Grujičić S, Gerothanassis IP, Tzakos AG, Stojanović ID. Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice. in Journal of Food Science. 2016;81(7):H1846-H1853.
doi:10.1111/1750-3841.13333 .

Vujičić, Milica, Nikolić, Ivana, Kontogianni, Vassiliki G., Saksida, Tamara, Charisiadis, Pantelis, Vasić, Bobana, Stošić-Grujičić, Stanislava, Gerothanassis, Ioannis P., Tzakos, Andreas G., Stojanović, Ivana D., "Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice" in Journal of Food Science, 81, no. 7 (2016):H1846-H1853,
https://doi.org/10.1111/1750-3841.13333 . .