TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617304486
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2832
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
SP  - 249
EP  - 262
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Gašić, Uroš and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
doi = "10.1016/j.jff.2017.07.060",
pages = "249-262"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Gašić, U., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods, 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Nikolovski N, Stanisavljević S, Gašić U, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Gašić, Uroš, Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Despotović, Jovana
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6050
AB  - Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.
PB  - American Chemical Society
T2  - Journal of Agricultural and Food Chemistry
T1  - Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells
IS  - 24
VL  - 64
DO  - 10.1021/acs.jafc.6b00951
SP  - 4900
EP  - 4907
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Despotović, Jovana and Miljković, Đorđe and Timotijević, Gordana",
year = "2016",
abstract = "Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.",
publisher = "American Chemical Society",
journal = "Journal of Agricultural and Food Chemistry",
title = "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells",
number = "24",
volume = "64",
doi = "10.1021/acs.jafc.6b00951",
pages = "4900-4907"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Despotović, J., Miljković, Đ.,& Timotijević, G.. (2016). Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry
American Chemical Society., 64(24), 4900-4907.
https://doi.org/10.1021/acs.jafc.6b00951

Jevtić B, Nikolovski N, Stanisavljević S, Despotović J, Miljković Đ, Timotijević G. Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry. 2016;64(24):4900-4907.
doi:10.1021/acs.jafc.6b00951 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Despotović, Jovana, Miljković, Đorđe, Timotijević, Gordana, "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells" in Journal of Agricultural and Food Chemistry, 64, no. 24 (2016):4900-4907,
https://doi.org/10.1021/acs.jafc.6b00951 . .