TY  - CONF
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Lević, Steva
AU  - Savić Šević, Svetlana
AU  - Pantelić, Dejan
AU  - Živić, Miroslav
AU  - Rabasović, Mihailo D
AU  - Krmpot, Aleksandar J
PY  - 2023
UR  - https://www.eventclass.org/contxt_emim2023/online-program/session?s=PW36#e609
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6289
AB  - Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
T1  - Laser nano-surgery of fungal cell wall to enable patch clamping
SP  - 1095
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6289
ER  - 

@conference{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Lević, Steva and Savić Šević, Svetlana and Pantelić, Dejan and Živić, Miroslav and Rabasović, Mihailo D and Krmpot, Aleksandar J",
year = "2023",
abstract = "Introduction
Electrophysiology studies of ion channels, in live filamentous fungi by patch clamp method are notpossible due to presence of rigid chitinous cell wall that prevents patch clamp pipette to access theplasma membrane. We present laser nano-surgery of the fungal cell wall that enables patch clampelectrophysiology studies. Similar approaches as one-time reports utilizing nanosecond laser pulseslong time ago were not pursued further [1,2]. Here, we demonstrate reproducible method usingfemtosecond lasers accompanied by two-photon excitation fluorescence (TPEF) imaging of hyphae.
Methods
A wild-type strain of filamentous fungus
Phycomyces blakesleeanus
(Burgeff ) [NRRL 1555(-)] were grownon glass coverslips with hand-etched grid, coated with a thin layer of 50% collagen type I as animmobilizer. Home built nonlinear laser scanning microscope [3,4] utilizing Ti:Sa tunable fs laser wasused for TPEF imaging of hyphae and the cell surgery. The latter is enabled with the custom made add-on in soft ware. Coverslip with hyphae is transferred to another microscope setup for patch clamp,consisting of micromanipulators and precise electronics for pA current measurements. The surgicalincisions and released protoplasts were additionally imaged by scanning electron microscopy for whichtreated hyphae had to undergo critical point drying procedure.
Results/Discussion
Hyphae were stained by Calcofluor White and treated with an exocytosis inhibitor (brefeldin A) and arespiration inhibitor (sodium azide) to prevent cell wall regeneration. Since the cell wall and the plasmamembrane are in the close contact [4] hyphae were kept in hyperosmotic solution to retract thecytoplasm from the cell wall. Surgical spot-wise pattern was precisely positioned at TPEF image ofselected hypha at the place where the plasma membrane was retracted. The dwell time (1s) and thelaser power (4-15mW) were set with fixed repetition rate (76MHz), pulse duration (160fs) and laserwavelength (730nm). Upon the surgery, hyphae were gently deplasmolysed. A protoplast with plasmamembrane accessible for the patch clamp pipette was released through the surgical incision (Fig 1). The>GΩ seal resistance was achieved. Numerous ion channels are recorded in diff erent configurations (oncell, inside-out, whole cell and out-out) (Fig 2).
Conclusions
The whole process (cell surgery + patch clamping) is rather complex and specific steps have to be strictlyfollowed for high success rate and reproducibility. Also, chemicals concentrations, solutions osmolarity,timing and cutting parameters have to be kept in the specified narrow range. Obtained currentrecordings provide valuable information on fungal cell membrane ionic channels.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria",
title = "Laser nano-surgery of fungal cell wall to enable patch clamping",
pages = "1095",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6289"
}

Pajić, T., Stevanović, K., Todorović, N., Lević, S., Savić Šević, S., Pantelić, D., Živić, M., Rabasović, M. D.,& Krmpot, A. J.. (2023). Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria
European Society for Molecular Imaging., 1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289

Pajić T, Stevanović K, Todorović N, Lević S, Savić Šević S, Pantelić D, Živić M, Rabasović MD, Krmpot AJ. Laser nano-surgery of fungal cell wall to enable patch clamping. in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria. 2023;:1095.
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Lević, Steva, Savić Šević, Svetlana, Pantelić, Dejan, Živić, Miroslav, Rabasović, Mihailo D, Krmpot, Aleksandar J, "Laser nano-surgery of fungal cell wall to enable patch clamping" in European Molecular Imaging Meeting: 18th Annual Meeting of the European Society for Molecular Imaging: EMIM 2023; 2023 Mar 14-17; Saltzburg, Austria (2023):1095,
https://hdl.handle.net/21.15107/rcub_ibiss_6289 .

TY  - JOUR
AU  - Pavlović, Danica
AU  - Vasiljević, Darko
AU  - Salatić, Branislav
AU  - Lazović, Vladimir
AU  - Dikić, Goran
AU  - Tomić, Ljubiša
AU  - Ćurčić, Srećko
AU  - Milovanović, Petar
AU  - Todorović, Dajana
AU  - Pantelić, Dejan V.
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0306456518301402?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3124
AB  - The insect cuticle serves a multitude of purposes, including: mechanical and thermal protection, water-repelling, acoustic signal absorption and coloration. The influence of cuticular structures on infrared radiation exchange and thermal balance is still largely unexplored. Here we report on the micro- and nanostructured setae covering the elytra of the longicorn beetle Rosalia alpina (Linnaeus, 1758) (Coleoptera: Cerambycidae) that help the insect to survive in hot, summer environments. In the visible part of the spectrum, scale-like setae, covering the black patches of the elytra, efficiently absorb light due to the radiation trap effect. In the infrared part of the spectrum, setae of the whole elytra significantly contribute to the radiative heat exchange. From the biological point of view, insect elytra facilitate camouflage, enable rapid heating to the optimum body temperature and prevent overheating by emitting excess thermal energy.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)
VL  - 76
DO  - 10.1016/J.JTHERBIO.2018.07.014
SP  - 126
EP  - 138
ER  - 

@article{
author = "Pavlović, Danica and Vasiljević, Darko and Salatić, Branislav and Lazović, Vladimir and Dikić, Goran and Tomić, Ljubiša and Ćurčić, Srećko and Milovanović, Petar and Todorović, Dajana and Pantelić, Dejan V.",
year = "2018",
abstract = "The insect cuticle serves a multitude of purposes, including: mechanical and thermal protection, water-repelling, acoustic signal absorption and coloration. The influence of cuticular structures on infrared radiation exchange and thermal balance is still largely unexplored. Here we report on the micro- and nanostructured setae covering the elytra of the longicorn beetle Rosalia alpina (Linnaeus, 1758) (Coleoptera: Cerambycidae) that help the insect to survive in hot, summer environments. In the visible part of the spectrum, scale-like setae, covering the black patches of the elytra, efficiently absorb light due to the radiation trap effect. In the infrared part of the spectrum, setae of the whole elytra significantly contribute to the radiative heat exchange. From the biological point of view, insect elytra facilitate camouflage, enable rapid heating to the optimum body temperature and prevent overheating by emitting excess thermal energy.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)",
volume = "76",
doi = "10.1016/J.JTHERBIO.2018.07.014",
pages = "126-138"
}

Pavlović, D., Vasiljević, D., Salatić, B., Lazović, V., Dikić, G., Tomić, L., Ćurčić, S., Milovanović, P., Todorović, D.,& Pantelić, D. V.. (2018). Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae). in Journal of Thermal Biology
Elsevier Ltd.., 76, 126-138.
https://doi.org/10.1016/J.JTHERBIO.2018.07.014

Pavlović D, Vasiljević D, Salatić B, Lazović V, Dikić G, Tomić L, Ćurčić S, Milovanović P, Todorović D, Pantelić DV. Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae). in Journal of Thermal Biology. 2018;76:126-138.
doi:10.1016/J.JTHERBIO.2018.07.014 .

Pavlović, Danica, Vasiljević, Darko, Salatić, Branislav, Lazović, Vladimir, Dikić, Goran, Tomić, Ljubiša, Ćurčić, Srećko, Milovanović, Petar, Todorović, Dajana, Pantelić, Dejan V., "Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)" in Journal of Thermal Biology, 76 (2018):126-138,
https://doi.org/10.1016/J.JTHERBIO.2018.07.014 . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Krmpot, Aleksandar
AU  - Rabasović, Mihailo
AU  - Lazović, Vladimir
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Živić, Miroslav
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6293
AB  - The ion channels on the membrane of filamentous fungi remain uninvestigated to this day due to their inaccessibility to patch-clamp pipette, brought about by sturdy cell wall. Small number of described channels is from very specific types of cells (wall less mutants or sporangiophore aerial cell membrane). The enzymatic approaches of cell wall removal, albeit successful on plant cells, failed when applied to fungi. In order to obtain clean “patchable” membrane from any type of filamentous fungal cell we undertook a task of finding the conditions for cut of the small part of the hyphal wall by laser surgery on the model filamentous fungus organism, Phycomyces blakesleeanus. The successful wall surgery should result in exposure of large enough portion of cell membrane with a minimal damage to the protoplast. Therefore, we performed series of experiments with cell plasmolisys in hypoosmotic media and subsequent deplasmolisys, to determine the conditions for reliable retraction of cytoplasm that could be reversed. Next, hyphae, grown on glass coverslips coated with collagen, were plasmolysed and mounted on the stage of the homemade nonlinear laser scanning microscope for imaging and cell surgery [1]. The Ti:Sapphire laser (Coherent, Mira 900-F) has been used as a light source in the microscope. It has operated at 730nm. This wavelength enables two photon excitation of auto-fluorescence in cytoplasm, as well as dye (Calcofluor white), visualizing fungi wall. We have used Carl Zeiss, EC Plan-NEOFLUAR, 40×1.3 oil immersion objective for focusing of the laser beam and collection of fluorescence. A visible interference filter (415nm - 685 nm) in front of detector has been used to remove scattered laser light. The successful cutting of cell wall could be achieved within the range of laser intensities and cutting speeds (dwell times). Throughout the experiment, fungi were kept in azide or Brefeldin A in order to block the process of depositing the new wall material. Afterwards, hyphae were slowly deplasmolysed to induce exit of a portion of the protoplast through the laser-cut hole in the cell wall. However, in some instances, the part of the protoplast bulged through the hole immediately after cell surgery, while the cell was still in hypertonic solution. In other instances, the cytoplasm remained away from the cut hyphal apex even through series of slow incrementing hypotonic solutions. Finally, when laser cutting was applied on the side of the cell only, as to cut a small hole, the successful exit of a portion of protoplast through the hole during deplasmolisys could be reliably achieved.
PB  - Belgrade: Institute of Physics
C3  - Book of Abstracts: the Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 & H2020-MSCA-RISE-2015 CARDIALLY workshop: PHOTONICA2017; 2017 Aug 28 - Sep 1; Belgrade, Serbia
T1  - Phycomyces blakesleeanus hypha cell wall surgery by Ti:Sapphire laser
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6293
ER  - 

@conference{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Krmpot, Aleksandar and Rabasović, Mihailo and Lazović, Vladimir and Pantelić, Dejan and Jelenković, Branislav and Živić, Miroslav",
year = "2017",
abstract = "The ion channels on the membrane of filamentous fungi remain uninvestigated to this day due to their inaccessibility to patch-clamp pipette, brought about by sturdy cell wall. Small number of described channels is from very specific types of cells (wall less mutants or sporangiophore aerial cell membrane). The enzymatic approaches of cell wall removal, albeit successful on plant cells, failed when applied to fungi. In order to obtain clean “patchable” membrane from any type of filamentous fungal cell we undertook a task of finding the conditions for cut of the small part of the hyphal wall by laser surgery on the model filamentous fungus organism, Phycomyces blakesleeanus. The successful wall surgery should result in exposure of large enough portion of cell membrane with a minimal damage to the protoplast. Therefore, we performed series of experiments with cell plasmolisys in hypoosmotic media and subsequent deplasmolisys, to determine the conditions for reliable retraction of cytoplasm that could be reversed. Next, hyphae, grown on glass coverslips coated with collagen, were plasmolysed and mounted on the stage of the homemade nonlinear laser scanning microscope for imaging and cell surgery [1]. The Ti:Sapphire laser (Coherent, Mira 900-F) has been used as a light source in the microscope. It has operated at 730nm. This wavelength enables two photon excitation of auto-fluorescence in cytoplasm, as well as dye (Calcofluor white), visualizing fungi wall. We have used Carl Zeiss, EC Plan-NEOFLUAR, 40×1.3 oil immersion objective for focusing of the laser beam and collection of fluorescence. A visible interference filter (415nm - 685 nm) in front of detector has been used to remove scattered laser light. The successful cutting of cell wall could be achieved within the range of laser intensities and cutting speeds (dwell times). Throughout the experiment, fungi were kept in azide or Brefeldin A in order to block the process of depositing the new wall material. Afterwards, hyphae were slowly deplasmolysed to induce exit of a portion of the protoplast through the laser-cut hole in the cell wall. However, in some instances, the part of the protoplast bulged through the hole immediately after cell surgery, while the cell was still in hypertonic solution. In other instances, the cytoplasm remained away from the cut hyphal apex even through series of slow incrementing hypotonic solutions. Finally, when laser cutting was applied on the side of the cell only, as to cut a small hole, the successful exit of a portion of protoplast through the hole during deplasmolisys could be reliably achieved.",
publisher = "Belgrade: Institute of Physics",
journal = "Book of Abstracts: the Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 & H2020-MSCA-RISE-2015 CARDIALLY workshop: PHOTONICA2017; 2017 Aug 28 - Sep 1; Belgrade, Serbia",
title = "Phycomyces blakesleeanus hypha cell wall surgery by Ti:Sapphire laser",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6293"
}

Pajić, T., Stevanović, K., Todorović, N., Krmpot, A., Rabasović, M., Lazović, V., Pantelić, D., Jelenković, B.,& Živić, M.. (2017). Phycomyces blakesleeanus hypha cell wall surgery by Ti:Sapphire laser. in Book of Abstracts: the Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 & H2020-MSCA-RISE-2015 CARDIALLY workshop: PHOTONICA2017; 2017 Aug 28 - Sep 1; Belgrade, Serbia
Belgrade: Institute of Physics., 106.
https://hdl.handle.net/21.15107/rcub_ibiss_6293

Pajić T, Stevanović K, Todorović N, Krmpot A, Rabasović M, Lazović V, Pantelić D, Jelenković B, Živić M. Phycomyces blakesleeanus hypha cell wall surgery by Ti:Sapphire laser. in Book of Abstracts: the Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 & H2020-MSCA-RISE-2015 CARDIALLY workshop: PHOTONICA2017; 2017 Aug 28 - Sep 1; Belgrade, Serbia. 2017;:106.
https://hdl.handle.net/21.15107/rcub_ibiss_6293 .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Krmpot, Aleksandar, Rabasović, Mihailo, Lazović, Vladimir, Pantelić, Dejan, Jelenković, Branislav, Živić, Miroslav, "Phycomyces blakesleeanus hypha cell wall surgery by Ti:Sapphire laser" in Book of Abstracts: the Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 & H2020-MSCA-RISE-2015 CARDIALLY workshop: PHOTONICA2017; 2017 Aug 28 - Sep 1; Belgrade, Serbia (2017):106,
https://hdl.handle.net/21.15107/rcub_ibiss_6293 .

TY  - CONF
AU  - Pavlović, Danica
AU  - Pantelić, Dejan
AU  - Vasiljević, Darko
AU  - Tomić, Ljubiša
AU  - Dikić, Goran
AU  - Todorović, Dajana
AU  - Petković, Branka
AU  - Prolić, Zlatko
PY  - 2017
UR  - https://docplayer.net/102264437-19th-international-conference-on-photoacoustic-and-photothermal-phenomena-july-16-20-bilbao-book-of-abstracts.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4004
AB  - Recently, thermal imaging has become an important sensing technology in biological investigations [1]. Here we present a thermo-optical analysis of a longhorn beetle’s exoskeleton (cuticle) in the visible and mid-infrared part of the spectrum. The insect (Morimus funereus, family Cerambycidae, shown in Fig. 1) possesses several prominent black patches on its hardened forewings (the elytrae). The patches, covered with dense, black, micron-sized hairs, were the subject of our investigation. Thermographic measurements were done by using a thermal camera, operating in the spectral range from 7.5 to 13 μm. We have found that deep black colour of the patches comes, not only from pigments (melanin), but from the underlying structure, and has a thermoregulatory effect [2]. Analysis has shown that these structures maximize absorption in a visible and minimize radiative thermal losses, thus enabling the insect to keep the heat. This particular property could be an inspiration for development of biomimetic coatings which will diminish thermal dissipation through radiation. This is in contrast to coatings inspired by an African desert ant, whose cuticle minimizes absorption in the visible and maximizes the radiative losses [3]. Principles discovered in both insects are well adjusted to different environments – a cold one, where it is important to keep heat, and a hot one, where it is necessary to dissipate thermal energy as efficiently as possible.
PB  - Bilbao, Spain: Dpto Física Aplicada I, Escuela de Ingeniería de Bilbao, Universidad del País Vasco UPV/EHU
C3  - 19th International Conference on Photoacoustic and Photothermal Phenomena
T1  - Thermo – optical properties of cuticular structures of Morimus funereus (Inescta: Cerambycidae)
SP  - 216
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4004
ER  - 

@conference{
author = "Pavlović, Danica and Pantelić, Dejan and Vasiljević, Darko and Tomić, Ljubiša and Dikić, Goran and Todorović, Dajana and Petković, Branka and Prolić, Zlatko",
year = "2017",
abstract = "Recently, thermal imaging has become an important sensing technology in biological investigations [1]. Here we present a thermo-optical analysis of a longhorn beetle’s exoskeleton (cuticle) in the visible and mid-infrared part of the spectrum. The insect (Morimus funereus, family Cerambycidae, shown in Fig. 1) possesses several prominent black patches on its hardened forewings (the elytrae). The patches, covered with dense, black, micron-sized hairs, were the subject of our investigation. Thermographic measurements were done by using a thermal camera, operating in the spectral range from 7.5 to 13 μm. We have found that deep black colour of the patches comes, not only from pigments (melanin), but from the underlying structure, and has a thermoregulatory effect [2]. Analysis has shown that these structures maximize absorption in a visible and minimize radiative thermal losses, thus enabling the insect to keep the heat. This particular property could be an inspiration for development of biomimetic coatings which will diminish thermal dissipation through radiation. This is in contrast to coatings inspired by an African desert ant, whose cuticle minimizes absorption in the visible and maximizes the radiative losses [3]. Principles discovered in both insects are well adjusted to different environments – a cold one, where it is important to keep heat, and a hot one, where it is necessary to dissipate thermal energy as efficiently as possible.",
publisher = "Bilbao, Spain: Dpto Física Aplicada I, Escuela de Ingeniería de Bilbao, Universidad del País Vasco UPV/EHU",
journal = "19th International Conference on Photoacoustic and Photothermal Phenomena",
title = "Thermo – optical properties of cuticular structures of Morimus funereus (Inescta: Cerambycidae)",
pages = "216",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4004"
}

Pavlović, D., Pantelić, D., Vasiljević, D., Tomić, L., Dikić, G., Todorović, D., Petković, B.,& Prolić, Z.. (2017). Thermo – optical properties of cuticular structures of Morimus funereus (Inescta: Cerambycidae). in 19th International Conference on Photoacoustic and Photothermal Phenomena
Bilbao, Spain: Dpto Física Aplicada I, Escuela de Ingeniería de Bilbao, Universidad del País Vasco UPV/EHU., 216.
https://hdl.handle.net/21.15107/rcub_ibiss_4004

Pavlović D, Pantelić D, Vasiljević D, Tomić L, Dikić G, Todorović D, Petković B, Prolić Z. Thermo – optical properties of cuticular structures of Morimus funereus (Inescta: Cerambycidae). in 19th International Conference on Photoacoustic and Photothermal Phenomena. 2017;:216.
https://hdl.handle.net/21.15107/rcub_ibiss_4004 .

Pavlović, Danica, Pantelić, Dejan, Vasiljević, Darko, Tomić, Ljubiša, Dikić, Goran, Todorović, Dajana, Petković, Branka, Prolić, Zlatko, "Thermo – optical properties of cuticular structures of Morimus funereus (Inescta: Cerambycidae)" in 19th International Conference on Photoacoustic and Photothermal Phenomena (2017):216,
https://hdl.handle.net/21.15107/rcub_ibiss_4004 .

TY  - JOUR
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2016
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/eea.12470
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3779
AB  - The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.
PB  - Blackwell Publishing Ltd.
T2  - Entomologia Experimentalis et Applicata
T1  - Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT
IS  - 2
VL  - 160
DO  - 10.1111/eea.12470
SP  - 188
EP  - 194
ER  - 

@article{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2016",
abstract = "The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.",
publisher = "Blackwell Publishing Ltd.",
journal = "Entomologia Experimentalis et Applicata",
title = "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT",
number = "2",
volume = "160",
doi = "10.1111/eea.12470",
pages = "188-194"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2016). Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata
Blackwell Publishing Ltd.., 160(2), 188-194.
https://doi.org/10.1111/eea.12470

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata. 2016;160(2):188-194.
doi:10.1111/eea.12470 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT" in Entomologia Experimentalis et Applicata, 160, no. 2 (2016):188-194,
https://doi.org/10.1111/eea.12470 . .

TY  - CONF
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4025
AB  - Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.
PB  - Belgrade: Entomological Society of Serbia
C3  - Simpozijum entomologa Srbije 2015
T1  - Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4025
ER  - 

@conference{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2015",
abstract = "Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.",
publisher = "Belgrade: Entomological Society of Serbia",
journal = "Simpozijum entomologa Srbije 2015",
title = "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4025"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2015). Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015
Belgrade: Entomological Society of Serbia., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015. 2015;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem" in Simpozijum entomologa Srbije 2015 (2015):17,
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .