TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Ukropina, Mirela
AU  - Cakić Milošević, Maja
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0300483X20302730
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4033
AB  - Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.
PB  - Elsevier Ireland Ltd
T2  - Toxicology
T2  - Toxicology
T1  - Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?
VL  - 447
DO  - 10.1016/j.tox.2020.152634
SP  - 152634
ER  - 

@article{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić Milošević, Maja and Glamočlija, Jasmina and Kataranovski, Milena and Mirkov, Ivana",
year = "2021",
abstract = "Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.",
publisher = "Elsevier Ireland Ltd",
journal = "Toxicology, Toxicology",
title = "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?",
volume = "447",
doi = "10.1016/j.tox.2020.152634",
pages = "152634"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Ukropina, M., Cakić Milošević, M., Glamočlija, J., Kataranovski, M.,& Mirkov, I.. (2021). Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology
Elsevier Ireland Ltd., 447, 152634.
https://doi.org/10.1016/j.tox.2020.152634

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Ukropina M, Cakić Milošević M, Glamočlija J, Kataranovski M, Mirkov I. Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology. 2021;447:152634.
doi:10.1016/j.tox.2020.152634 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić Milošević, Maja, Glamočlija, Jasmina, Kataranovski, Milena, Mirkov, Ivana, "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?" in Toxicology, 447 (2021):152634,
https://doi.org/10.1016/j.tox.2020.152634 . .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Ukropina, Mirela
AU  - Cakić Milošević, Maja
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2019
UR  - http://www.besjournal.com/en/article/doi/10.3967/bes2019.068
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3470
AB  - OBJECTIVE The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. METHODS Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. RESULTS Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. CONCLUSION The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.
PB  - Biomedical and Environmental Sciences
T2  - Biomedical and Environmental Sciences
T1  - Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats.
IS  - 7
VL  - 32
DO  - 10.3967/bes2019.068
SP  - 508
EP  - 519
ER  - 

@article{
author = "Kulaš, Jelena and Ninkov, Marina and Tucović, Dina and Popov Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić Milošević, Maja and Mutić, Jelena and Kataranovski, Milena and Mirkov, Ivana",
year = "2019",
abstract = "OBJECTIVE The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. METHODS Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. RESULTS Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. CONCLUSION The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.",
publisher = "Biomedical and Environmental Sciences",
journal = "Biomedical and Environmental Sciences",
title = "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats.",
number = "7",
volume = "32",
doi = "10.3967/bes2019.068",
pages = "508-519"
}

Kulaš, J., Ninkov, M., Tucović, D., Popov Aleksandrov, A., Ukropina, M., Cakić Milošević, M., Mutić, J., Kataranovski, M.,& Mirkov, I.. (2019). Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats.. in Biomedical and Environmental Sciences
Biomedical and Environmental Sciences., 32(7), 508-519.
https://doi.org/10.3967/bes2019.068

Kulaš J, Ninkov M, Tucović D, Popov Aleksandrov A, Ukropina M, Cakić Milošević M, Mutić J, Kataranovski M, Mirkov I. Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats.. in Biomedical and Environmental Sciences. 2019;32(7):508-519.
doi:10.3967/bes2019.068 .

Kulaš, Jelena, Ninkov, Marina, Tucović, Dina, Popov Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić Milošević, Maja, Mutić, Jelena, Kataranovski, Milena, Mirkov, Ivana, "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats." in Biomedical and Environmental Sciences, 32, no. 7 (2019):508-519,
https://doi.org/10.3967/bes2019.068 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Filipović, Branko
AU  - Ivanović, Ana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0944200617300521
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2900
AB  - Vertebral morphology, development, and evolution have been investigated for many decades, especially in the recent evo-devo era. Nevertheless, comparative data on development and ossification modes within the major tetrapod groups are scarce and frequently suffer from the use of a simplistic approach, resulting in simplistic generalizations about the formation of tetrapod vertebrae. Here, we describe the development and ossification of trunk vertebrae in Triturus ivanbureschi (Salamandridae, Caudata) and compare the results with published data on other related taxa. In so doing, we focus on the modes of ossification and development of the centrum and neural arches by analysing three developmental stages defined by the degree of limb development: stages 47, 52, and 62 according to Glu¨cksohn (1932). Our examination of histological sections through trunk vertebrae enabled us to identify three modes of ossification within single trunk vertebrae: (i) perichordal (direct ossification of the connective tissue surrounding the notochord); (ii) perichondrial (direct ossification of the perichondrium, consisting of cartilage-covering connective tissue), and (iii) endochondral (ossification within the preformed cartilage template). We also noted the presence of intravertebral or notochordal cartilage. Although our results indicate that this cartilage develops within the notochord surrounded by the continuous notochordal sheath, more detailed further studies could shed light on its origin and development.
T2  - Zoology
T1  - Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)
VL  - 128
DO  - 10.1016/j.zool.2017.10.001
SP  - 164
EP  - 171
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Filipović, Branko and Ivanović, Ana",
year = "2018",
abstract = "Vertebral morphology, development, and evolution have been investigated for many decades, especially in the recent evo-devo era. Nevertheless, comparative data on development and ossification modes within the major tetrapod groups are scarce and frequently suffer from the use of a simplistic approach, resulting in simplistic generalizations about the formation of tetrapod vertebrae. Here, we describe the development and ossification of trunk vertebrae in Triturus ivanbureschi (Salamandridae, Caudata) and compare the results with published data on other related taxa. In so doing, we focus on the modes of ossification and development of the centrum and neural arches by analysing three developmental stages defined by the degree of limb development: stages 47, 52, and 62 according to Glu¨cksohn (1932). Our examination of histological sections through trunk vertebrae enabled us to identify three modes of ossification within single trunk vertebrae: (i) perichordal (direct ossification of the connective tissue surrounding the notochord); (ii) perichondrial (direct ossification of the perichondrium, consisting of cartilage-covering connective tissue), and (iii) endochondral (ossification within the preformed cartilage template). We also noted the presence of intravertebral or notochordal cartilage. Although our results indicate that this cartilage develops within the notochord surrounded by the continuous notochordal sheath, more detailed further studies could shed light on its origin and development.",
journal = "Zoology",
title = "Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)",
volume = "128",
doi = "10.1016/j.zool.2017.10.001",
pages = "164-171"
}

Ajduković, M., Ukropina, M., Filipović, B.,& Ivanović, A.. (2018). Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata). in Zoology, 128, 164-171.
https://doi.org/10.1016/j.zool.2017.10.001

Ajduković M, Ukropina M, Filipović B, Ivanović A. Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata). in Zoology. 2018;128:164-171.
doi:10.1016/j.zool.2017.10.001 .

Ajduković, Maja, Ukropina, Mirela, Filipović, Branko, Ivanović, Ana, "Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)" in Zoology, 128 (2018):164-171,
https://doi.org/10.1016/j.zool.2017.10.001 . .

TY  - JOUR
AU  - Petrović-Kosanović, Dragana
AU  - Ukropina, Mirela
AU  - Čakić-Milošević, Maja
AU  - Budeč, Mirela
AU  - Milošević, Verica
AU  - Koko, Vesna
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/329
AB  - Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.
T2  - Archives of Biological Sciences
T1  - Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress
IS  - 1
VL  - 65
SP  - 315
EP  - 320
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_329
ER  - 

@article{
author = "Petrović-Kosanović, Dragana and Ukropina, Mirela and Čakić-Milošević, Maja and Budeč, Mirela and Milošević, Verica and Koko, Vesna",
year = "2013, 2013",
abstract = "Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.",
journal = "Archives of Biological Sciences",
title = "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress",
number = "1",
volume = "65",
pages = "315-320",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_329"
}

Petrović-Kosanović, D., Ukropina, M., Čakić-Milošević, M., Budeč, M., Milošević, V.,& Koko, V.. (2013). Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences, 65(1), 315-320.
https://hdl.handle.net/21.15107/rcub_ibiss_329

Petrović-Kosanović D, Ukropina M, Čakić-Milošević M, Budeč M, Milošević V, Koko V. Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences. 2013;65(1):315-320.
https://hdl.handle.net/21.15107/rcub_ibiss_329 .

Petrović-Kosanović, Dragana, Ukropina, Mirela, Čakić-Milošević, Maja, Budeč, Mirela, Milošević, Verica, Koko, Vesna, "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress" in Archives of Biological Sciences, 65, no. 1 (2013):315-320,
https://hdl.handle.net/21.15107/rcub_ibiss_329 .