TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Ćurčić, Marijana
AU  - Baralić, Katarina
AU  - Kolarević, Stoimir
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
AU  - Buha Đorđević, Aleksandra
AU  - Vuković-Gačić, Branka
AU  - Javorac, Dragana
AU  - Antonijević Miljaković, Evica
AU  - Carević, Momir
AU  - Mandinić, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5166
AB  - Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment.
PB  - Elsevier Ltd.
T2  - Environmental Pollution
T1  - Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.
VL  - 314
DO  - 10.1016/j.envpol.2022.120321
SP  - 120321
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Ćurčić, Marijana and Baralić, Katarina and Kolarević, Stoimir and Bulat, Zorica and Đukić-Ćosić, Danijela and Buha Đorđević, Aleksandra and Vuković-Gačić, Branka and Javorac, Dragana and Antonijević Miljaković, Evica and Carević, Momir and Mandinić, Zoran",
year = "2022",
abstract = "Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment.",
publisher = "Elsevier Ltd.",
journal = "Environmental Pollution",
title = "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.",
volume = "314",
doi = "10.1016/j.envpol.2022.120321",
pages = "120321"
}

Radovanović, J., Antonijević, B., Ćurčić, M., Baralić, K., Kolarević, S., Bulat, Z., Đukić-Ćosić, D., Buha Đorđević, A., Vuković-Gačić, B., Javorac, D., Antonijević Miljaković, E., Carević, M.,& Mandinić, Z.. (2022). Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.. in Environmental Pollution
Elsevier Ltd.., 314, 120321.
https://doi.org/10.1016/j.envpol.2022.120321

Radovanović J, Antonijević B, Ćurčić M, Baralić K, Kolarević S, Bulat Z, Đukić-Ćosić D, Buha Đorđević A, Vuković-Gačić B, Javorac D, Antonijević Miljaković E, Carević M, Mandinić Z. Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.. in Environmental Pollution. 2022;314:120321.
doi:10.1016/j.envpol.2022.120321 .

Radovanović, Jelena, Antonijević, Biljana, Ćurčić, Marijana, Baralić, Katarina, Kolarević, Stoimir, Bulat, Zorica, Đukić-Ćosić, Danijela, Buha Đorđević, Aleksandra, Vuković-Gačić, Branka, Javorac, Dragana, Antonijević Miljaković, Evica, Carević, Momir, Mandinić, Zoran, "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage." in Environmental Pollution, 314 (2022):120321,
https://doi.org/10.1016/j.envpol.2022.120321 . .