TY  - JOUR
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Tucić, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6032
AB  - Phenotypic plasticity is widely acknowledged as one of the most common solutions for coping with novel environmental conditions following climate change. However, it is less known whether the current amounts of trait plasticity, which is sufficient for matching with the contemporary climate, will be adequate when global temperatures exceed historical levels. We addressed this issue by exploring the responses of functional and structural leaf traits in Iris pumila clonal individuals to experimentally increased temperatures (~1.5 °C) using an open top chamber (OTC) design. We determined the phenotypic values of the specific leaf area, leaf dry matter content, specific leaf water content, and leaf thickness in the leaves sampled from the same clone inside and outside of the OTC deployed on it, over seasons and years within two natural populations. We analyzed the data using a repeated multivariate analysis of variance, which primarily focusses on the profiles (reaction norms (RNs)) of a variable gathered from the same individual at several different time points. We found that the mean RNs of all analyzed traits were parallel regardless of experienced temperatures, but differed in the level and the shape. The populations RNs were similar as well. As the amount of plasticity in the analyzed leaf trait was adequate for coping with elevated temperatures inside the OTCs, we predict that it will be also sufficient for responding to increased temperatures if they exceed the 1.5 °C target.
PB  - Basel : MDPI
T2  - Plants
T1  - Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.
IS  - 17
VL  - 12
DO  - 10.3390/plants12173114
SP  - 3114
ER  - 

@article{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Vuleta, Ana and Tucić, Branka",
year = "2023",
abstract = "Phenotypic plasticity is widely acknowledged as one of the most common solutions for coping with novel environmental conditions following climate change. However, it is less known whether the current amounts of trait plasticity, which is sufficient for matching with the contemporary climate, will be adequate when global temperatures exceed historical levels. We addressed this issue by exploring the responses of functional and structural leaf traits in Iris pumila clonal individuals to experimentally increased temperatures (~1.5 °C) using an open top chamber (OTC) design. We determined the phenotypic values of the specific leaf area, leaf dry matter content, specific leaf water content, and leaf thickness in the leaves sampled from the same clone inside and outside of the OTC deployed on it, over seasons and years within two natural populations. We analyzed the data using a repeated multivariate analysis of variance, which primarily focusses on the profiles (reaction norms (RNs)) of a variable gathered from the same individual at several different time points. We found that the mean RNs of all analyzed traits were parallel regardless of experienced temperatures, but differed in the level and the shape. The populations RNs were similar as well. As the amount of plasticity in the analyzed leaf trait was adequate for coping with elevated temperatures inside the OTCs, we predict that it will be also sufficient for responding to increased temperatures if they exceed the 1.5 °C target.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.",
number = "17",
volume = "12",
doi = "10.3390/plants12173114",
pages = "3114"
}

Manitašević Jovanović, S., Hočevar, K., Vuleta, A.,& Tucić, B.. (2023). Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.. in Plants
Basel : MDPI., 12(17), 3114.
https://doi.org/10.3390/plants12173114

Manitašević Jovanović S, Hočevar K, Vuleta A, Tucić B. Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.. in Plants. 2023;12(17):3114.
doi:10.3390/plants12173114 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Vuleta, Ana, Tucić, Branka, "Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L." in Plants, 12, no. 17 (2023):3114,
https://doi.org/10.3390/plants12173114 . .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Tucić, Branka
AU  - Klingenberg, Christian Peter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4997
AB  - Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.
PB  - Hoboken : John Wiley & Sons Ltd
T2  - Evolution & Development
T1  - Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry
DO  - 10.1111/ede.12402
ER  - 

@article{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Tucić, Branka and Klingenberg, Christian Peter",
year = "2022",
abstract = "Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "Evolution & Development",
title = "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry",
doi = "10.1111/ede.12402"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Tucić, B.,& Klingenberg, C. P.. (2022). Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development
Hoboken : John Wiley & Sons Ltd..
https://doi.org/10.1111/ede.12402

Budečević S, Manitašević Jovanović S, Vuleta A, Tucić B, Klingenberg CP. Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development. 2022;.
doi:10.1111/ede.12402 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Tucić, Branka, Klingenberg, Christian Peter, "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry" in Evolution & Development (2022),
https://doi.org/10.1111/ede.12402 . .

TY  - CONF
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Hočevar, Katarina
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5061
AB  - Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5061
ER  - 

@conference{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Hočevar, Katarina and Tucić, Branka",
year = "2018",
abstract = "Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5061"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Hočevar, K.,& Tucić, B.. (2018). Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061

Budečević S, Manitašević Jovanović S, Vuleta A, Hočevar K, Tucić B. Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Hočevar, Katarina, Tucić, Branka, "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):82,
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5062
AB  - Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Do pollinators prefer bigger floral organs? A case study on Iris pumila L.
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5062
ER  - 

@conference{
author = "Vuleta, Ana and Budečević, Sanja and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2018",
abstract = "Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Do pollinators prefer bigger floral organs? A case study on Iris pumila L.",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5062"
}

Vuleta, A., Budečević, S., Manitašević Jovanović, S.,& Tucić, B.. (2018). Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062

Vuleta A, Budečević S, Manitašević Jovanović S, Tucić B. Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

Vuleta, Ana, Budečević, Sanja, Manitašević Jovanović, Sanja, Tucić, Branka, "Do pollinators prefer bigger floral organs? A case study on Iris pumila L." in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):86,
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Klingenberg, Christian Peter
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jeb.13207
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29134739
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2927
AB  - Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.
T2  - Journal of Evolutionary Biology
T1  - Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.
IS  - 2
VL  - 31
DO  - 10.1111/jeb.13207
SP  - 197
EP  - 210
ER  - 

@article{
author = "Tucić, Branka and Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Klingenberg, Christian Peter",
year = "2018",
abstract = "Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.",
journal = "Journal of Evolutionary Biology",
title = "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.",
number = "2",
volume = "31",
doi = "10.1111/jeb.13207",
pages = "197-210"
}

Tucić, B., Budečević, S., Manitašević Jovanović, S., Vuleta, A.,& Klingenberg, C. P.. (2018). Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology, 31(2), 197-210.
https://doi.org/10.1111/jeb.13207

Tucić B, Budečević S, Manitašević Jovanović S, Vuleta A, Klingenberg CP. Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology. 2018;31(2):197-210.
doi:10.1111/jeb.13207 .

Tucić, Branka, Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Klingenberg, Christian Peter, "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence." in Journal of Evolutionary Biology, 31, no. 2 (2018):197-210,
https://doi.org/10.1111/jeb.13207 . .

TY  - JOUR
AU  - Radović, Sanja
AU  - Urošević, Aleksandar
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600086R
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/838
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2772
AB  - The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.
T2  - Archives of Biological Sciences
T1  - Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations
IS  - 2
VL  - 69
DO  - 10.2298/ABS160912086R
SP  - 223
EP  - 231
ER  - 

@article{
author = "Radović, Sanja and Urošević, Aleksandar and Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2017",
abstract = "The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.",
journal = "Archives of Biological Sciences",
title = "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations",
number = "2",
volume = "69",
doi = "10.2298/ABS160912086R",
pages = "223-231"
}

Radović, S., Urošević, A., Hočevar, K., Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2017). Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences, 69(2), 223-231.
https://doi.org/10.2298/ABS160912086R

Radović S, Urošević A, Hočevar K, Vuleta A, Manitašević Jovanović S, Tucić B. Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences. 2017;69(2):223-231.
doi:10.2298/ABS160912086R .

Radović, Sanja, Urošević, Aleksandar, Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations" in Archives of Biological Sciences, 69, no. 2 (2017):223-231,
https://doi.org/10.2298/ABS160912086R . .

TY  - GEN
AU  - Radović, Sanja
AU  - Urošević, Aleksandar
AU  - Hocevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600086R
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2599
AB  - The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects’ landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units. [Projekat Ministarstva nauke Republike Srbije, br. 173007]
T2  - Archives of Biological Sciences
T1  - Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations
DO  - 10.2298/ABS160912086R
ER  - 

@misc{
author = "Radović, Sanja and Urošević, Aleksandar and Hocevar, Katarina and Vuleta, Ana and Manitašević-Jovanović, Sanja and Tucić, Branka",
year = "2016",
abstract = "The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects’ landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units. [Projekat Ministarstva nauke Republike Srbije, br. 173007]",
journal = "Archives of Biological Sciences",
title = "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations",
doi = "10.2298/ABS160912086R"
}

Radović, S., Urošević, A., Hocevar, K., Vuleta, A., Manitašević-Jovanović, S.,& Tucić, B.. (2016). Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences.
https://doi.org/10.2298/ABS160912086R

Radović S, Urošević A, Hocevar K, Vuleta A, Manitašević-Jovanović S, Tucić B. Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences. 2016;.
doi:10.2298/ABS160912086R .

Radović, Sanja, Urošević, Aleksandar, Hocevar, Katarina, Vuleta, Ana, Manitašević-Jovanović, Sanja, Tucić, Branka, "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations" in Archives of Biological Sciences (2016),
https://doi.org/10.2298/ABS160912086R . .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26841194
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0981942816300109
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2597
AB  - High solar radiation has been recognized as one of the main causes of the overproduction of reactive oxygen species (ROS) and oxidative stress in plants. To remove the excess of ROS, plants use different antioxidants and tune their activity and/or isoform number as required for given light conditions. In this study, the adaptiveness of light-induced variation in the activities and isoform patterns of key enzymatic antioxidants SOD, APX and CAT was tested in leaves of Iris pumila clonal plants from two natural populations inhabiting a sun exposed dune site and a forest understory, using a reciprocal-transplant experiment. At the exposed habitat, the mean enzymatic activity of total SODs was significantly greater than that in the shaded one, while the amount of the mitochondrial MnSOD was notably higher compared to the plastidic Cu/ZnSOD. However, the number of Cu/ZnSOD isoforms was greater in the forest understory relative to the exposed site (three vs. two, respectively). An inverse relationship recorded between the quantities of MnSOD and Cu/ZnSOD in alternative light habitats might indicate that the two enzymes compensate each other in maintaining intracellular ROS and redox balance. The adaptive population differentiation in APX activity was exclusively recorded in the open habitat, which indicated that the synergistic effect of high light and temperature stress could be the principal selective factor, rather than high light alone. The enzymatic activity of CAT was similar between the two populations, implicating APX as the primary H2O2 scavenger in the I. pumila leaves exposed to high light intensity.
T2  - Plant Physiology and Biochemistry
T1  - Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case
VL  - 100
DO  - 10.1016/j.plaphy.2016.01.011
SP  - 166
EP  - 173
ER  - 

@article{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2016",
abstract = "High solar radiation has been recognized as one of the main causes of the overproduction of reactive oxygen species (ROS) and oxidative stress in plants. To remove the excess of ROS, plants use different antioxidants and tune their activity and/or isoform number as required for given light conditions. In this study, the adaptiveness of light-induced variation in the activities and isoform patterns of key enzymatic antioxidants SOD, APX and CAT was tested in leaves of Iris pumila clonal plants from two natural populations inhabiting a sun exposed dune site and a forest understory, using a reciprocal-transplant experiment. At the exposed habitat, the mean enzymatic activity of total SODs was significantly greater than that in the shaded one, while the amount of the mitochondrial MnSOD was notably higher compared to the plastidic Cu/ZnSOD. However, the number of Cu/ZnSOD isoforms was greater in the forest understory relative to the exposed site (three vs. two, respectively). An inverse relationship recorded between the quantities of MnSOD and Cu/ZnSOD in alternative light habitats might indicate that the two enzymes compensate each other in maintaining intracellular ROS and redox balance. The adaptive population differentiation in APX activity was exclusively recorded in the open habitat, which indicated that the synergistic effect of high light and temperature stress could be the principal selective factor, rather than high light alone. The enzymatic activity of CAT was similar between the two populations, implicating APX as the primary H2O2 scavenger in the I. pumila leaves exposed to high light intensity.",
journal = "Plant Physiology and Biochemistry",
title = "Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case",
volume = "100",
doi = "10.1016/j.plaphy.2016.01.011",
pages = "166-173"
}

Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. in Plant Physiology and Biochemistry, 100, 166-173.
https://doi.org/10.1016/j.plaphy.2016.01.011

Vuleta A, Manitašević Jovanović S, Tucić B. Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. in Plant Physiology and Biochemistry. 2016;100:166-173.
doi:10.1016/j.plaphy.2016.01.011 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case" in Plant Physiology and Biochemistry, 100 (2016):166-173,
https://doi.org/10.1016/j.plaphy.2016.01.011 . .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucic, Branka
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2101
AB  - Oxidative stress results from incongruity between the generation of
   toxic reactive oxygen species (ROS) and the availability of their
   scavengers-antioxidants. Although the short-term effects of this
   phenomenon are attracting much scientific attention, oxidative stress
   may influence an organism's metabolism over the long (evolutionary) time
   scale as well. To disentangle the impact of strong light intensity from
   co-occurring abiotic stresses in creating adaptive responses in
   antioxidants and heat shock proteins (Hsps), an environment manipulation
   experiment was performed using a xerophyte clonal monocot, Iris pumila,
   native to semi-arid grasslands at the Deliblato Sands. This species is
   very tolerant to the combined effect of extreme abiotic stressors such
   as high light intensity, elevated soil surface temperatures, and
   scarcity of water, which commonly takes place in its natural habitats
   during the summer. By shading half of each selected clone, leaving the
   other half sun-exposed, we contrasted short-term effects of reduced
   daylight intensity with long-term effects of photo-oxidative stress. In
   both light treatments, the enzymatic activities of SOD and APX
   antioxidants were similar in magnitude, whereas those of CAT and POD
   significantly decreased in exposed compared to shaded leaves. Moreover,
   exposed leaves expressed a unique CAT isoform that differed
   biochemically from two CAT isoforms observed in shaded leaves. The
   content of non-enzymatic antioxidants, carotenoids (Car), remained
   constant with the reduction of light intensity, but their ratio to total
   chlorophylls (Chl) significantly decreased compared to that expressed in
   full sunlight. The abundance of Hsps was considerably greater in exposed
   than in shaded leaves, especially regarding the inducible isoforms,
   Hsp70 and Hsp90a, as were their proportions in relation to the
   constitutively expressed Hsp90b isoform. The presented results, thus,
   indicate that adaptive metabolic responses of I. pumila leaves to
   photo-oxidative stress entailed the high activity of two key enzymatic
   antioxidants, SOD and APX and the expression of a light-resistant CAT-to
   counteract the stress-mediated ROS accumulation, the increased Car to
   Chl ratio-to adjust the photosynthetic apparatus to the high light
   conditions, as well as the accelerated biosynthesis of heat shock
   proteins Hsp70 and Hsp90-to preserve the cellular proteostasis.
T2  - Acta Physiologiae Plantarum
T1  - How do plants cope with oxidative stress in nature? A study on the dwarf
 bearded iris (Iris pumila)
IS  - 1711
VL  - 37
DO  - 10.1007/s11738-014-1711-9
ER  - 

@article{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Tucic, Branka",
year = "2015",
abstract = "Oxidative stress results from incongruity between the generation of
   toxic reactive oxygen species (ROS) and the availability of their
   scavengers-antioxidants. Although the short-term effects of this
   phenomenon are attracting much scientific attention, oxidative stress
   may influence an organism's metabolism over the long (evolutionary) time
   scale as well. To disentangle the impact of strong light intensity from
   co-occurring abiotic stresses in creating adaptive responses in
   antioxidants and heat shock proteins (Hsps), an environment manipulation
   experiment was performed using a xerophyte clonal monocot, Iris pumila,
   native to semi-arid grasslands at the Deliblato Sands. This species is
   very tolerant to the combined effect of extreme abiotic stressors such
   as high light intensity, elevated soil surface temperatures, and
   scarcity of water, which commonly takes place in its natural habitats
   during the summer. By shading half of each selected clone, leaving the
   other half sun-exposed, we contrasted short-term effects of reduced
   daylight intensity with long-term effects of photo-oxidative stress. In
   both light treatments, the enzymatic activities of SOD and APX
   antioxidants were similar in magnitude, whereas those of CAT and POD
   significantly decreased in exposed compared to shaded leaves. Moreover,
   exposed leaves expressed a unique CAT isoform that differed
   biochemically from two CAT isoforms observed in shaded leaves. The
   content of non-enzymatic antioxidants, carotenoids (Car), remained
   constant with the reduction of light intensity, but their ratio to total
   chlorophylls (Chl) significantly decreased compared to that expressed in
   full sunlight. The abundance of Hsps was considerably greater in exposed
   than in shaded leaves, especially regarding the inducible isoforms,
   Hsp70 and Hsp90a, as were their proportions in relation to the
   constitutively expressed Hsp90b isoform. The presented results, thus,
   indicate that adaptive metabolic responses of I. pumila leaves to
   photo-oxidative stress entailed the high activity of two key enzymatic
   antioxidants, SOD and APX and the expression of a light-resistant CAT-to
   counteract the stress-mediated ROS accumulation, the increased Car to
   Chl ratio-to adjust the photosynthetic apparatus to the high light
   conditions, as well as the accelerated biosynthesis of heat shock
   proteins Hsp70 and Hsp90-to preserve the cellular proteostasis.",
journal = "Acta Physiologiae Plantarum",
title = "How do plants cope with oxidative stress in nature? A study on the dwarf
 bearded iris (Iris pumila)",
number = "1711",
volume = "37",
doi = "10.1007/s11738-014-1711-9"
}

Vuleta, A., Manitašević Jovanović, S.,& Tucic, B.. (2015). How do plants cope with oxidative stress in nature? A study on the dwarf
 bearded iris (Iris pumila). in Acta Physiologiae Plantarum, 37(1711).
https://doi.org/10.1007/s11738-014-1711-9

Vuleta A, Manitašević Jovanović S, Tucic B. How do plants cope with oxidative stress in nature? A study on the dwarf
 bearded iris (Iris pumila). in Acta Physiologiae Plantarum. 2015;37(1711).
doi:10.1007/s11738-014-1711-9 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Tucic, Branka, "How do plants cope with oxidative stress in nature? A study on the dwarf
 bearded iris (Iris pumila)" in Acta Physiologiae Plantarum, 37, no. 1711 (2015),
https://doi.org/10.1007/s11738-014-1711-9 . .

TY  - JOUR
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Tucic, Branka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2272
AB  - We tested the hypothesis that sharing essential resources through
   interconnected ramets increases stress tolerance of a clonal plant in
   patchy environments. A number of circle-shaped clones of Iris pumila
   naturally growing in a sun-exposed habitat were selected and cut into
   two equal halves with different integration status: one with intact and
   the other with disconnected rhizomes. One-half of each clone was then
   shaded with a neutral screen to provide 50\% of ambient irradiance, so
   that one-half of both clones consisted of connected and disconnected
   halves (referred to as ``clone quarters{''}). Leaves collected from each
   clone quarter were analyzed for malondialdehyde (MDA) content (a stress
   indicator) and the values of three functional traits, specific leaf area
   (SLA), leaf dry matter content (LDMC) and leaf water content (LWC). MDA
   content was greater in unshaded (more stressed) than in shaded (less
   stressed) leaves. All three morphological traits changed with light
   gradient, but only SLA was impacted by the ramet integration status. SLA
   and MDA tended to be inversely related in each clone quarter, indicating
   a (compensatory) mechanism utilized by I. pumila plants in coping with
   environmental stress. SLA and LDMC were highly negatively correlated in
   general, but less strongly in interconnected ramets compared to those
   with a disrupted connection. The results suggest that intraclonal
   physiological integration confers a fitness benefit to I. pumila plants
   in heterogeneous environments, likely by balancing two fundamental plant
   activities rapid biomass production and nutrient conservation.
T2  - Archives of Biological Sciences
T1  - Does physiological integration among intraclonal ramets of Iris pumila enhance stress tolerance in heterogeneous environments?
IS  - 2
VL  - 66
DO  - 10.2298/ABS1402713J
SP  - 713
EP  - 720
ER  - 

@article{
author = "Manitašević Jovanović, Sanja and Vuleta, Ana and Tucic, Branka",
year = "2014",
abstract = "We tested the hypothesis that sharing essential resources through
   interconnected ramets increases stress tolerance of a clonal plant in
   patchy environments. A number of circle-shaped clones of Iris pumila
   naturally growing in a sun-exposed habitat were selected and cut into
   two equal halves with different integration status: one with intact and
   the other with disconnected rhizomes. One-half of each clone was then
   shaded with a neutral screen to provide 50\% of ambient irradiance, so
   that one-half of both clones consisted of connected and disconnected
   halves (referred to as ``clone quarters{''}). Leaves collected from each
   clone quarter were analyzed for malondialdehyde (MDA) content (a stress
   indicator) and the values of three functional traits, specific leaf area
   (SLA), leaf dry matter content (LDMC) and leaf water content (LWC). MDA
   content was greater in unshaded (more stressed) than in shaded (less
   stressed) leaves. All three morphological traits changed with light
   gradient, but only SLA was impacted by the ramet integration status. SLA
   and MDA tended to be inversely related in each clone quarter, indicating
   a (compensatory) mechanism utilized by I. pumila plants in coping with
   environmental stress. SLA and LDMC were highly negatively correlated in
   general, but less strongly in interconnected ramets compared to those
   with a disrupted connection. The results suggest that intraclonal
   physiological integration confers a fitness benefit to I. pumila plants
   in heterogeneous environments, likely by balancing two fundamental plant
   activities rapid biomass production and nutrient conservation.",
journal = "Archives of Biological Sciences",
title = "Does physiological integration among intraclonal ramets of Iris pumila enhance stress tolerance in heterogeneous environments?",
number = "2",
volume = "66",
doi = "10.2298/ABS1402713J",
pages = "713-720"
}

Manitašević Jovanović, S., Vuleta, A.,& Tucic, B.. (2014). Does physiological integration among intraclonal ramets of Iris pumila enhance stress tolerance in heterogeneous environments?. in Archives of Biological Sciences, 66(2), 713-720.
https://doi.org/10.2298/ABS1402713J

Manitašević Jovanović S, Vuleta A, Tucic B. Does physiological integration among intraclonal ramets of Iris pumila enhance stress tolerance in heterogeneous environments?. in Archives of Biological Sciences. 2014;66(2):713-720.
doi:10.2298/ABS1402713J .

Manitašević Jovanović, Sanja, Vuleta, Ana, Tucic, Branka, "Does physiological integration among intraclonal ramets of Iris pumila enhance stress tolerance in heterogeneous environments?" in Archives of Biological Sciences, 66, no. 2 (2014):713-720,
https://doi.org/10.2298/ABS1402713J . .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Tucić, Branka
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6207
AB  - Irradiance is the one of the most variable abiotic factors experienced by higher plants. Adaptive plastic
responses to fluctuating light conditions have been documented to affect all aspects of plant phenotype
– morphology, physiology and biochemistry. In order to examine the influence of light intensity on the
plasticity of biochemical traits, a random sample of ten Iris pumila clones inhabiting an exposed site in
the Deliblato Sands were partially covered with a neutral screen that transmitted 50% of daylight so
that half of the ramets of a single clone experienced reduced and the other half full exposure to
sunlight. In summer, leaf samples were collected and analyzed for the following biochemical traits: the
relative levels of heat stress proteins (Hsp70 and two isoforms of Hsp90 – inducible Hsp90a and
constitutive Hsp90b); the specific activities of antioxidant enzymes (SOD, CAT, POD, APX, GR); the
contents of non-enzymatic antioxidants (carotenoids), and the extent of lipid peroxidation (MDA
content). The relative levels of all three Hsps (in particular Hsp70 and Hsp90a) were elevated in the
unshaded ramets when compared to their shaded counterparts. In addition, the oxidative stress index
(OSI), expressed in terms of the ratio of MDA to the sum of the geometric means of all examined
antioxidants, was shown to be about 30% greater in ramets from the exposed clone parts than in ramets
from the shaded ones. Of all of the traits analyzed, Hsp70, Hsp90a and OSI exhibited higher values for
the plasticity indices (PIv: 0.44, 0.53 and 0.37, respectively), pointing to their substantial role in coping
with high light intensity. Conversely, Hsp90b appeared to be the least plastic trait (PIv: 0.21), which is
in agreement with its constitutive function. Our results provide evidence that I. pumila displays a
biochemical plastic response that enables overcoming fitness reduction under environmental stress that
commonly occurs in their natural habitats.
PB  - Basel: European Society for Evolutionary Biology
C3  - Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
T1  - Biochemical plasticity and environmental stress: Iris pumila as a case study
SP  - 272
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6207
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Vuleta, Ana and Tucić, Branka",
year = "2013",
abstract = "Irradiance is the one of the most variable abiotic factors experienced by higher plants. Adaptive plastic
responses to fluctuating light conditions have been documented to affect all aspects of plant phenotype
– morphology, physiology and biochemistry. In order to examine the influence of light intensity on the
plasticity of biochemical traits, a random sample of ten Iris pumila clones inhabiting an exposed site in
the Deliblato Sands were partially covered with a neutral screen that transmitted 50% of daylight so
that half of the ramets of a single clone experienced reduced and the other half full exposure to
sunlight. In summer, leaf samples were collected and analyzed for the following biochemical traits: the
relative levels of heat stress proteins (Hsp70 and two isoforms of Hsp90 – inducible Hsp90a and
constitutive Hsp90b); the specific activities of antioxidant enzymes (SOD, CAT, POD, APX, GR); the
contents of non-enzymatic antioxidants (carotenoids), and the extent of lipid peroxidation (MDA
content). The relative levels of all three Hsps (in particular Hsp70 and Hsp90a) were elevated in the
unshaded ramets when compared to their shaded counterparts. In addition, the oxidative stress index
(OSI), expressed in terms of the ratio of MDA to the sum of the geometric means of all examined
antioxidants, was shown to be about 30% greater in ramets from the exposed clone parts than in ramets
from the shaded ones. Of all of the traits analyzed, Hsp70, Hsp90a and OSI exhibited higher values for
the plasticity indices (PIv: 0.44, 0.53 and 0.37, respectively), pointing to their substantial role in coping
with high light intensity. Conversely, Hsp90b appeared to be the least plastic trait (PIv: 0.21), which is
in agreement with its constitutive function. Our results provide evidence that I. pumila displays a
biochemical plastic response that enables overcoming fitness reduction under environmental stress that
commonly occurs in their natural habitats.",
publisher = "Basel: European Society for Evolutionary Biology",
journal = "Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal",
title = "Biochemical plasticity and environmental stress: Iris pumila as a case study",
pages = "272",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6207"
}

Manitašević Jovanović, S., Vuleta, A.,& Tucić, B.. (2013). Biochemical plasticity and environmental stress: Iris pumila as a case study. in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
Basel: European Society for Evolutionary Biology., 272.
https://hdl.handle.net/21.15107/rcub_ibiss_6207

Manitašević Jovanović S, Vuleta A, Tucić B. Biochemical plasticity and environmental stress: Iris pumila as a case study. in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal. 2013;:272.
https://hdl.handle.net/21.15107/rcub_ibiss_6207 .

Manitašević Jovanović, Sanja, Vuleta, Ana, Tucić, Branka, "Biochemical plasticity and environmental stress: Iris pumila as a case study" in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal (2013):272,
https://hdl.handle.net/21.15107/rcub_ibiss_6207 .

TY  - CONF
AU  - Tucić, Branka
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6208
AB  - Natural populations of the dwarf bearded iris, Iris pumila, display a striking flower-colour
polymorphism. It was noted that the combination of fluctuating temperatures and the varied
attractiveness of diverse colour morphs promotes a stable coexistence of multiple colour variants in a
population. We have quantified the amounts of Hsp90 and the antioxidants, anthocyanins and total
phenolics, in I. pumila flowers. These molecules impact abiotic stress tolerance, ultimately influencing
the fitness of individual plants. A total of 100 clones that were raised in a common garden and assessed
to different colour classes (dark violet, violet, light violet, dark blue, light blue, yellow/white) were
examined. The amounts of two Hsp90 forms, inducible (Hsp90a) and constitutively expressed
(Hsp90b) proteins were lowest in yellow and white flowers as compared to other colour classes. In blue
flowers, the concentration of Hsp90a was observed to decrease gradually when proceeding from light
blue to dark blue variants, whereas an inverse trend was observed in violet-coloured flowers. The
concentration of anthocyanins was notably low in white/yellow flowers and in the blue and violet
colour classes it progressively increased from light to dark floral morphs. The amounts of total
phenolics were highest in the white and yellow colour morphs; they were relatively high in all of the
blue variants, and gradually increase from light to dark colour morphs in the violet class. These results
suggest that each I. pumila colour genotype is responsible for the production of unique amounts of
Hsp90 and phenolics that protect cellular homeostasis under fluctuating temperature conditions within
populations.
PB  - Basel: European Society for Evolutionary Biology
C3  - Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
T1  - Flower colour morphs of Iris pumila differ in the amounts of HSP90 and phenolic compounds
SP  - 810
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6208
ER  - 

@conference{
author = "Tucić, Branka and Manitašević Jovanović, Sanja and Vuleta, Ana",
year = "2013",
abstract = "Natural populations of the dwarf bearded iris, Iris pumila, display a striking flower-colour
polymorphism. It was noted that the combination of fluctuating temperatures and the varied
attractiveness of diverse colour morphs promotes a stable coexistence of multiple colour variants in a
population. We have quantified the amounts of Hsp90 and the antioxidants, anthocyanins and total
phenolics, in I. pumila flowers. These molecules impact abiotic stress tolerance, ultimately influencing
the fitness of individual plants. A total of 100 clones that were raised in a common garden and assessed
to different colour classes (dark violet, violet, light violet, dark blue, light blue, yellow/white) were
examined. The amounts of two Hsp90 forms, inducible (Hsp90a) and constitutively expressed
(Hsp90b) proteins were lowest in yellow and white flowers as compared to other colour classes. In blue
flowers, the concentration of Hsp90a was observed to decrease gradually when proceeding from light
blue to dark blue variants, whereas an inverse trend was observed in violet-coloured flowers. The
concentration of anthocyanins was notably low in white/yellow flowers and in the blue and violet
colour classes it progressively increased from light to dark floral morphs. The amounts of total
phenolics were highest in the white and yellow colour morphs; they were relatively high in all of the
blue variants, and gradually increase from light to dark colour morphs in the violet class. These results
suggest that each I. pumila colour genotype is responsible for the production of unique amounts of
Hsp90 and phenolics that protect cellular homeostasis under fluctuating temperature conditions within
populations.",
publisher = "Basel: European Society for Evolutionary Biology",
journal = "Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal",
title = "Flower colour morphs of Iris pumila differ in the amounts of HSP90 and phenolic compounds",
pages = "810",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6208"
}

Tucić, B., Manitašević Jovanović, S.,& Vuleta, A.. (2013). Flower colour morphs of Iris pumila differ in the amounts of HSP90 and phenolic compounds. in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
Basel: European Society for Evolutionary Biology., 810.
https://hdl.handle.net/21.15107/rcub_ibiss_6208

Tucić B, Manitašević Jovanović S, Vuleta A. Flower colour morphs of Iris pumila differ in the amounts of HSP90 and phenolic compounds. in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal. 2013;:810.
https://hdl.handle.net/21.15107/rcub_ibiss_6208 .

Tucić, Branka, Manitašević Jovanović, Sanja, Vuleta, Ana, "Flower colour morphs of Iris pumila differ in the amounts of HSP90 and phenolic compounds" in Abstract Book: 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal (2013):810,
https://hdl.handle.net/21.15107/rcub_ibiss_6208 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2013
UR  - http://www.eseb2013.com/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4994
AB  - For plants, sunlight is not only the major source of energy for photosynthesis, but also a powerful
evolutionary force that shapes the evolutionary trajectories of many traits. The aim of this study was to
examine seasonal- and habitat-dependent variations in the specific activities of different antioxidants in
the leaves of Iris pumila clones expressed under contrasting light conditions in the wild. Two
populations were selected in the Deliblato Sands: one experiencing full sunlight and one from a
woodland understory. Leaf samples were collected in spring, summer and autumn from the same clones
in each population. The specific activities of the antioxidative enzymes, SOD, APX, CAT, GR and
POD, and the contents of non-enzymatic antioxidants, anthocyanins and phenolics, were determined.
All of the examined antioxidants were observed to be upregulated in summer-harvested leaves when
compared to their spring or autumn counterparts. This suggests that reinforcement of the antioxidants
might be the key mechanism for acclimatization of I. pumila leaves to seasonal variations in light
intensity. In both populations, the antioxidative enzymes SOD, GR and POD expressed a greater mean
seasonal plasticity than the other antioxidants. However, the between-population difference was
significant only for POD. To elucidate whether variations in antioxidants reflect adaptations to local
environments, a reciprocal transplant experiment was conducted in the wild. The higher activities of
antioxidative enzymes were accompanied by increased contents of non-enzymatic antioxidants in both
populations in the open habitat in comparison to clones in the shaded habitat. This could be a reflection
of adaptive plasticity to the synergistic effect of strong light and high temperature. In contrast to
seasonal plasticity, habitat-dependent plasticity peaked for APX and CAT in both populations. Apart
from the phenolic content, a significant between-population difference was not observed.
PB  - European Society for Evolutionary Biology
C3  - 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
T1  - Plasticity of antioxidant defence responses to abiotic stress in natural populations of Iris pumila
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4994
ER  - 

@conference{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2013",
abstract = "For plants, sunlight is not only the major source of energy for photosynthesis, but also a powerful
evolutionary force that shapes the evolutionary trajectories of many traits. The aim of this study was to
examine seasonal- and habitat-dependent variations in the specific activities of different antioxidants in
the leaves of Iris pumila clones expressed under contrasting light conditions in the wild. Two
populations were selected in the Deliblato Sands: one experiencing full sunlight and one from a
woodland understory. Leaf samples were collected in spring, summer and autumn from the same clones
in each population. The specific activities of the antioxidative enzymes, SOD, APX, CAT, GR and
POD, and the contents of non-enzymatic antioxidants, anthocyanins and phenolics, were determined.
All of the examined antioxidants were observed to be upregulated in summer-harvested leaves when
compared to their spring or autumn counterparts. This suggests that reinforcement of the antioxidants
might be the key mechanism for acclimatization of I. pumila leaves to seasonal variations in light
intensity. In both populations, the antioxidative enzymes SOD, GR and POD expressed a greater mean
seasonal plasticity than the other antioxidants. However, the between-population difference was
significant only for POD. To elucidate whether variations in antioxidants reflect adaptations to local
environments, a reciprocal transplant experiment was conducted in the wild. The higher activities of
antioxidative enzymes were accompanied by increased contents of non-enzymatic antioxidants in both
populations in the open habitat in comparison to clones in the shaded habitat. This could be a reflection
of adaptive plasticity to the synergistic effect of strong light and high temperature. In contrast to
seasonal plasticity, habitat-dependent plasticity peaked for APX and CAT in both populations. Apart
from the phenolic content, a significant between-population difference was not observed.",
publisher = "European Society for Evolutionary Biology",
journal = "14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal",
title = "Plasticity of antioxidant defence responses to abiotic stress in natural populations of Iris pumila",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4994"
}

Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2013). Plasticity of antioxidant defence responses to abiotic stress in natural populations of Iris pumila. in 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal
European Society for Evolutionary Biology..
https://hdl.handle.net/21.15107/rcub_ibiss_4994

Vuleta A, Manitašević Jovanović S, Tucić B. Plasticity of antioxidant defence responses to abiotic stress in natural populations of Iris pumila. in 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal. 2013;.
https://hdl.handle.net/21.15107/rcub_ibiss_4994 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Plasticity of antioxidant defence responses to abiotic stress in natural populations of Iris pumila" in 14th Congress of the European Society for Evolutionary Biology; 2013 Aug 19-24; Lisbon, Portugal (2013),
https://hdl.handle.net/21.15107/rcub_ibiss_4994 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/342
AB  - The angiosperm flower is a complex integrated phenotype, but within this structure there are partly independent units or modules. The interconnections among floral organ traits are hypothesized to be mostly generated by pollinatormediated selection. In this study, we explore whether floral dry mass per area (DMA) in an insect-pollinated herb, Iris pumila, exhibits a modular correlation pattern as has been reported for some size-related traits. We found that the overall pattern of floral organ integration with regard to DMA was uneven in the offspring of Iris pumila derived from a sunexposed and a shaded natural population. Since principal component analysis (PCA) showed that most of the eigenvalue variance was explained by the first two principal components (PCs), these PCs were considered as two floral modules. The greatest factor loadings on the first PC axis was that of the perianth and style arm DMA (PSDMA) and perianth tube DMA (PTDMA),while on the second PC axis, the greatest factor loading was that of stamen DMA (STDMA). The results indicate that the function of the first module would be to attract a pollinating vector, while the second one would reflect male functions. Selection analyses revealed that the targets of phenotypic selection were both intra-floral integration and individual floral traits. Both PSDMA and PC1DMA were under strong linear selection, while PTDMA experienced direct stabilization selection. The level of integration in floral organ DMA expressed in the term of relative eigenvalue variance appeared to be rather low, as was documented for other angiosperm taxa.
T2  - Archives of Biological Sciences
T1  - Exploring phenotypic floral integration in Iris pumila L.: A common-garden experiment
IS  - 2
VL  - 65
SP  - 781
EP  - 793
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_342
ER  - 

@article{
author = "Tucić, Branka and Vuleta, Ana and Manitašević-Jovanović, Sanja",
year = "2013, 2013",
abstract = "The angiosperm flower is a complex integrated phenotype, but within this structure there are partly independent units or modules. The interconnections among floral organ traits are hypothesized to be mostly generated by pollinatormediated selection. In this study, we explore whether floral dry mass per area (DMA) in an insect-pollinated herb, Iris pumila, exhibits a modular correlation pattern as has been reported for some size-related traits. We found that the overall pattern of floral organ integration with regard to DMA was uneven in the offspring of Iris pumila derived from a sunexposed and a shaded natural population. Since principal component analysis (PCA) showed that most of the eigenvalue variance was explained by the first two principal components (PCs), these PCs were considered as two floral modules. The greatest factor loadings on the first PC axis was that of the perianth and style arm DMA (PSDMA) and perianth tube DMA (PTDMA),while on the second PC axis, the greatest factor loading was that of stamen DMA (STDMA). The results indicate that the function of the first module would be to attract a pollinating vector, while the second one would reflect male functions. Selection analyses revealed that the targets of phenotypic selection were both intra-floral integration and individual floral traits. Both PSDMA and PC1DMA were under strong linear selection, while PTDMA experienced direct stabilization selection. The level of integration in floral organ DMA expressed in the term of relative eigenvalue variance appeared to be rather low, as was documented for other angiosperm taxa.",
journal = "Archives of Biological Sciences",
title = "Exploring phenotypic floral integration in Iris pumila L.: A common-garden experiment",
number = "2",
volume = "65",
pages = "781-793",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_342"
}

Tucić, B., Vuleta, A.,& Manitašević-Jovanović, S.. (2013). Exploring phenotypic floral integration in Iris pumila L.: A common-garden experiment. in Archives of Biological Sciences, 65(2), 781-793.
https://hdl.handle.net/21.15107/rcub_ibiss_342

Tucić B, Vuleta A, Manitašević-Jovanović S. Exploring phenotypic floral integration in Iris pumila L.: A common-garden experiment. in Archives of Biological Sciences. 2013;65(2):781-793.
https://hdl.handle.net/21.15107/rcub_ibiss_342 .

Tucić, Branka, Vuleta, Ana, Manitašević-Jovanović, Sanja, "Exploring phenotypic floral integration in Iris pumila L.: A common-garden experiment" in Archives of Biological Sciences, 65, no. 2 (2013):781-793,
https://hdl.handle.net/21.15107/rcub_ibiss_342 .

TY  - JOUR
AU  - Manitašević-Jovanović, Sanja
AU  - Tucić, Branka
AU  - Matić, Gordana
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1318
AB  - Tissue-specific variation in Hsp70 and Hsp90 expression was studied in vegetative (leaf) and reproductive organs (floral tube, ovary and stamen) of Iris pumila plants originating from a sun-exposed and a shaded natural population, which experienced similar growth conditions in an experimental garden. Western blot analysis revealed the presence of both the Hsps in all examined tissues, but at different amounts. In addition to Hsp90a and Hsp90b that were previously detected in vegetative tissues, three new immunospecific bands, designated herein as Hsp90c, Hsp90d and Hsp90e, were recognized with the same anti-Hsp90 antibody in the reproductive tissues. Apart from showing tissue-specific differences in the relative amount of Hsp70 and Hsp90, our study provides evidence that the degree of Hsps expression within the same tissue also depended on the habitat type that the I. pumila plants were derived from.
T2  - Acta Physiologiae Plantarum
T1  - Differential expression of heat-shock proteins Hsp70 and Hsp90 in vegetative and reproductive tissues of Iris pumila
IS  - 1
VL  - 33
EP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1318
ER  - 

@article{
author = "Manitašević-Jovanović, Sanja and Tucić, Branka and Matić, Gordana",
year = "2011",
abstract = "Tissue-specific variation in Hsp70 and Hsp90 expression was studied in vegetative (leaf) and reproductive organs (floral tube, ovary and stamen) of Iris pumila plants originating from a sun-exposed and a shaded natural population, which experienced similar growth conditions in an experimental garden. Western blot analysis revealed the presence of both the Hsps in all examined tissues, but at different amounts. In addition to Hsp90a and Hsp90b that were previously detected in vegetative tissues, three new immunospecific bands, designated herein as Hsp90c, Hsp90d and Hsp90e, were recognized with the same anti-Hsp90 antibody in the reproductive tissues. Apart from showing tissue-specific differences in the relative amount of Hsp70 and Hsp90, our study provides evidence that the degree of Hsps expression within the same tissue also depended on the habitat type that the I. pumila plants were derived from.",
journal = "Acta Physiologiae Plantarum",
title = "Differential expression of heat-shock proteins Hsp70 and Hsp90 in vegetative and reproductive tissues of Iris pumila",
number = "1",
volume = "33",
pages = "240",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1318"
}

Manitašević-Jovanović, S., Tucić, B.,& Matić, G.. (2011). Differential expression of heat-shock proteins Hsp70 and Hsp90 in vegetative and reproductive tissues of Iris pumila. in Acta Physiologiae Plantarum, 33(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1318

Manitašević-Jovanović S, Tucić B, Matić G. Differential expression of heat-shock proteins Hsp70 and Hsp90 in vegetative and reproductive tissues of Iris pumila. in Acta Physiologiae Plantarum. 2011;33(1):null-240.
https://hdl.handle.net/21.15107/rcub_ibiss_1318 .

Manitašević-Jovanović, Sanja, Tucić, Branka, Matić, Gordana, "Differential expression of heat-shock proteins Hsp70 and Hsp90 in vegetative and reproductive tissues of Iris pumila" in Acta Physiologiae Plantarum, 33, no. 1 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1318 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/285
AB  - Ambient light significantly influences the structural and physiological characteristics of Iris pumila leaves. A random sample of Iris clones native to an exposed site at the Deliblato Sands, Serbia was partially covered with a neutral screen that transmitted 35% of daylight, so that each clone experienced reduced and full sunlight at the same time. The sun-exposed leaves were significantly thicker, had greater stomatal density, exhibited higher lipid peroxidation, increased activities of SOD, APX, CAT enzymes and higher contents of non-enzymatic antioxidants (anthocyanins and phenols) and water deficit relative to shade-leaves. The activities of GR, GPX, and GST enzymes was unaffected by the irradiance level.
T2  - Archives of Biological Sciences
T1  - Light intensity influences variations in the structural and physiological traits in the leaves of Iris pumila L.
IS  - 4
VL  - 63
SP  - 1099
EP  - 1110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_285
ER  - 

@article{
author = "Vuleta, Ana and Manitašević-Jovanović, Sanja and Tucić, Branka",
year = "2011, 2011",
abstract = "Ambient light significantly influences the structural and physiological characteristics of Iris pumila leaves. A random sample of Iris clones native to an exposed site at the Deliblato Sands, Serbia was partially covered with a neutral screen that transmitted 35% of daylight, so that each clone experienced reduced and full sunlight at the same time. The sun-exposed leaves were significantly thicker, had greater stomatal density, exhibited higher lipid peroxidation, increased activities of SOD, APX, CAT enzymes and higher contents of non-enzymatic antioxidants (anthocyanins and phenols) and water deficit relative to shade-leaves. The activities of GR, GPX, and GST enzymes was unaffected by the irradiance level.",
journal = "Archives of Biological Sciences",
title = "Light intensity influences variations in the structural and physiological traits in the leaves of Iris pumila L.",
number = "4",
volume = "63",
pages = "1099-1110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_285"
}

Vuleta, A., Manitašević-Jovanović, S.,& Tucić, B.. (2011). Light intensity influences variations in the structural and physiological traits in the leaves of Iris pumila L.. in Archives of Biological Sciences, 63(4), 1099-1110.
https://hdl.handle.net/21.15107/rcub_ibiss_285

Vuleta A, Manitašević-Jovanović S, Tucić B. Light intensity influences variations in the structural and physiological traits in the leaves of Iris pumila L.. in Archives of Biological Sciences. 2011;63(4):1099-1110.
https://hdl.handle.net/21.15107/rcub_ibiss_285 .

Vuleta, Ana, Manitašević-Jovanović, Sanja, Tucić, Branka, "Light intensity influences variations in the structural and physiological traits in the leaves of Iris pumila L." in Archives of Biological Sciences, 63, no. 4 (2011):1099-1110,
https://hdl.handle.net/21.15107/rcub_ibiss_285 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/281
AB  - Plasticity to irradiance and genotypic correlations between structural and physiological leaf traits in Iris pumila were investigated in an experiment conducted at a sun-exposed dune habitat in the Deliblato Sands. A sample of six native, genetically different clones were covered with a neutral screen which transmitted ~35% of daylight, so that one clone-half of each clone experienced reduced sunlight, while the other one full sunlight. LMA, stomatal density, Ψleaf, enzymatic and non-enzymatic antioxidants and lipid peroxidation were determined in unshaded and shaded leaves of the same clone. It was found that the plasticity index, PIv was higher for physiological than for structural traits. Genotypic correlations between trait pairs were high, but rarely significant, in contrast to the correlation matrices which were significantly different between unshaded and shaded leaves.
T2  - Archives of Biological Sciences
T1  - Pattern of plasticity to irradiance levels and genotypic correlations between structural and physiological leaf traits in Iris pumila
IS  - 3
VL  - 63
SP  - 655
EP  - 660
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_281
ER  - 

@article{
author = "Vuleta, Ana and Manitašević-Jovanović, Sanja and Tucić, Branka",
year = "2011, 2011",
abstract = "Plasticity to irradiance and genotypic correlations between structural and physiological leaf traits in Iris pumila were investigated in an experiment conducted at a sun-exposed dune habitat in the Deliblato Sands. A sample of six native, genetically different clones were covered with a neutral screen which transmitted ~35% of daylight, so that one clone-half of each clone experienced reduced sunlight, while the other one full sunlight. LMA, stomatal density, Ψleaf, enzymatic and non-enzymatic antioxidants and lipid peroxidation were determined in unshaded and shaded leaves of the same clone. It was found that the plasticity index, PIv was higher for physiological than for structural traits. Genotypic correlations between trait pairs were high, but rarely significant, in contrast to the correlation matrices which were significantly different between unshaded and shaded leaves.",
journal = "Archives of Biological Sciences",
title = "Pattern of plasticity to irradiance levels and genotypic correlations between structural and physiological leaf traits in Iris pumila",
number = "3",
volume = "63",
pages = "655-660",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_281"
}

Vuleta, A., Manitašević-Jovanović, S.,& Tucić, B.. (2011). Pattern of plasticity to irradiance levels and genotypic correlations between structural and physiological leaf traits in Iris pumila. in Archives of Biological Sciences, 63(3), 655-660.
https://hdl.handle.net/21.15107/rcub_ibiss_281

Vuleta A, Manitašević-Jovanović S, Tucić B. Pattern of plasticity to irradiance levels and genotypic correlations between structural and physiological leaf traits in Iris pumila. in Archives of Biological Sciences. 2011;63(3):655-660.
https://hdl.handle.net/21.15107/rcub_ibiss_281 .

Vuleta, Ana, Manitašević-Jovanović, Sanja, Tucić, Branka, "Pattern of plasticity to irradiance levels and genotypic correlations between structural and physiological leaf traits in Iris pumila" in Archives of Biological Sciences, 63, no. 3 (2011):655-660,
https://hdl.handle.net/21.15107/rcub_ibiss_281 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
AU  - Tucić, Branka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5895
AB  - Plants in their natural habitats frequently cope with a multitude of abiotic stresses, such as high light intensity, extreme temperatures and water deficit, which often co-occur during periods of drought, especially in semi-arid and arid regions. Exposure of plants to stressful environmental conditions usually induce overproduction of reactive oxygen species (ROS) that, as highly toxic derivatives of O2, can assault all cell macromolecules, leading to the disruption of cellular homeostasis and, consequently, the uncoupling of major metabolic processes, the photosynthesis and photorespiration. In order to minimize ROS-mediated cellular damage, plants have evolved highly efficient antioxidative defense systems that include both enzymatic and non-enzymatic components. Since abiotic stress can also operate as a strong evolutionary force that shapes adaptations in natural plant populations, the aim of this study was to examine the seasonal variation patterns of two distinct antioxidative systems, ROS-scavenging enzymes and anthocyanin pigments, in the leaf tissue of a steppe plant, Iris pumila, as expressed under contrasting light conditions that the species regularly experiences in the wild.
PB  - Oxford, UK: Oxford University Press
T2  - Journal of Plant Ecology
T1  - Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.
IS  - 1
VL  - 3
DO  - 10.1093/jpe/rtp019
SP  - 59
EP  - 69
ER  - 

@article{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka and Tucić, Branka",
year = "2010",
abstract = "Plants in their natural habitats frequently cope with a multitude of abiotic stresses, such as high light intensity, extreme temperatures and water deficit, which often co-occur during periods of drought, especially in semi-arid and arid regions. Exposure of plants to stressful environmental conditions usually induce overproduction of reactive oxygen species (ROS) that, as highly toxic derivatives of O2, can assault all cell macromolecules, leading to the disruption of cellular homeostasis and, consequently, the uncoupling of major metabolic processes, the photosynthesis and photorespiration. In order to minimize ROS-mediated cellular damage, plants have evolved highly efficient antioxidative defense systems that include both enzymatic and non-enzymatic components. Since abiotic stress can also operate as a strong evolutionary force that shapes adaptations in natural plant populations, the aim of this study was to examine the seasonal variation patterns of two distinct antioxidative systems, ROS-scavenging enzymes and anthocyanin pigments, in the leaf tissue of a steppe plant, Iris pumila, as expressed under contrasting light conditions that the species regularly experiences in the wild.",
publisher = "Oxford, UK: Oxford University Press",
journal = "Journal of Plant Ecology",
title = "Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.",
number = "1",
volume = "3",
doi = "10.1093/jpe/rtp019",
pages = "59-69"
}

Vuleta, A., Manitašević Jovanović, S., Šešlija Jovanović, D.,& Tucić, B.. (2010). Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.. in Journal of Plant Ecology
Oxford, UK: Oxford University Press., 3(1), 59-69.
https://doi.org/10.1093/jpe/rtp019

Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D, Tucić B. Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.. in Journal of Plant Ecology. 2010;3(1):59-69.
doi:10.1093/jpe/rtp019 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, Tucić, Branka, "Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L." in Journal of Plant Ecology, 3, no. 1 (2010):59-69,
https://doi.org/10.1093/jpe/rtp019 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Šešlija-Jovanović, Darka M.
AU  - Tucić, Branka
AU  - Tucić, Nikola G
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1333
AB  - The level of homosexual behaviour is evaluated in one laboratory population of seed beetle and derived lines selected to reproduce early (E) or late in life (L), where inadvertent selection for either low or high heterosexual activity has been detected. The magnitudes of homosexual interaction, measured as chasing and mounting individuals of the same sex, are estimated over different age classes. These magnitudes are correlated with previously observed levels and patterns of age-specific variation of heterosexual activity of both sexes in the E and L experimental lines. The results obtained support the perception error hypothesis proposing that a low degree of sexual discrimination is genetically correlated with high sexual activity. The fitness costs of the same-sex interactions are tested by assessing their effects on longevity. In both sexes, the longevities of homosexual pairs are reduced relative to individually-housed virgin beetles in both the E and L lines, although homosexual interactions have a more pronounced effect on male survival than on female survival. Although the results obtained suggest that the longevity cost of homosexual interactions can be substantial, this cost is much smaller than the cost of heterosexual interactions.
T2  - Physiological Entomology
T1  - Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus
IS  - 4
VL  - 35
EP  - 316
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1333
ER  - 

@article{
author = "Stojković, Biljana and Šešlija-Jovanović, Darka M. and Tucić, Branka and Tucić, Nikola G",
year = "2010",
abstract = "The level of homosexual behaviour is evaluated in one laboratory population of seed beetle and derived lines selected to reproduce early (E) or late in life (L), where inadvertent selection for either low or high heterosexual activity has been detected. The magnitudes of homosexual interaction, measured as chasing and mounting individuals of the same sex, are estimated over different age classes. These magnitudes are correlated with previously observed levels and patterns of age-specific variation of heterosexual activity of both sexes in the E and L experimental lines. The results obtained support the perception error hypothesis proposing that a low degree of sexual discrimination is genetically correlated with high sexual activity. The fitness costs of the same-sex interactions are tested by assessing their effects on longevity. In both sexes, the longevities of homosexual pairs are reduced relative to individually-housed virgin beetles in both the E and L lines, although homosexual interactions have a more pronounced effect on male survival than on female survival. Although the results obtained suggest that the longevity cost of homosexual interactions can be substantial, this cost is much smaller than the cost of heterosexual interactions.",
journal = "Physiological Entomology",
title = "Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus",
number = "4",
volume = "35",
pages = "316",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1333"
}

Stojković, B., Šešlija-Jovanović, D. M., Tucić, B.,& Tucić, N. G.. (2010). Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus. in Physiological Entomology, 35(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1333

Stojković B, Šešlija-Jovanović DM, Tucić B, Tucić NG. Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus. in Physiological Entomology. 2010;35(4):null-316.
https://hdl.handle.net/21.15107/rcub_ibiss_1333 .

Stojković, Biljana, Šešlija-Jovanović, Darka M., Tucić, Branka, Tucić, Nikola G, "Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus" in Physiological Entomology, 35, no. 4 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1333 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Miljković, Danijela
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1352
AB  - Fluctuating asymmetry (FA), the subtle, random difference between the left and right side of bilateral structures, has often been used as a tool for evaluating developmental instability (DI) in natural populations subjected to environmental stresses. A general assumption underlying these studies is that the level of DI should increase with stress intensity. We examined the level of floral FA in six natural populations of Iris pumila experiencing sun-exposed (more stressful) and shaded (less stressful) environmental conditions. We used two single-trait indices (size-dependent FA1 and size-scaled FA8a) and one multi-trait index (FA17) to assess the FA levels in three floral traits: fall width, standard width and style branch width. Although floral FA was present in all FAs and appeared to be greater in plants from full sunlight than in those beneath vegetation canopy, only the FA17 index detected a significant FA-stress association. Although the FA1 index had no statistical power to reveal differences in the floral FA at any of the hierarchical levels studied, between alternative light habitats, among populations from comparable environmental conditions, among individual clones within each population or between different floral organs of a single flower, the FA8a index detected significant between-trait variation in the degree of floral FA within the same Iris individuals.
T2  - Plant Species Biology
T1  - Fluctuating asymmetry of floral organ traits in natural populations of Iris pumila from contrasting light habitats
IS  - 3
VL  - 25
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1352
ER  - 

@article{
author = "Tucić, Branka and Miljković, Danijela",
year = "2010",
abstract = "Fluctuating asymmetry (FA), the subtle, random difference between the left and right side of bilateral structures, has often been used as a tool for evaluating developmental instability (DI) in natural populations subjected to environmental stresses. A general assumption underlying these studies is that the level of DI should increase with stress intensity. We examined the level of floral FA in six natural populations of Iris pumila experiencing sun-exposed (more stressful) and shaded (less stressful) environmental conditions. We used two single-trait indices (size-dependent FA1 and size-scaled FA8a) and one multi-trait index (FA17) to assess the FA levels in three floral traits: fall width, standard width and style branch width. Although floral FA was present in all FAs and appeared to be greater in plants from full sunlight than in those beneath vegetation canopy, only the FA17 index detected a significant FA-stress association. Although the FA1 index had no statistical power to reveal differences in the floral FA at any of the hierarchical levels studied, between alternative light habitats, among populations from comparable environmental conditions, among individual clones within each population or between different floral organs of a single flower, the FA8a index detected significant between-trait variation in the degree of floral FA within the same Iris individuals.",
journal = "Plant Species Biology",
title = "Fluctuating asymmetry of floral organ traits in natural populations of Iris pumila from contrasting light habitats",
number = "3",
volume = "25",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1352"
}

Tucić, B.,& Miljković, D.. (2010). Fluctuating asymmetry of floral organ traits in natural populations of Iris pumila from contrasting light habitats. in Plant Species Biology, 25(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1352

Tucić B, Miljković D. Fluctuating asymmetry of floral organ traits in natural populations of Iris pumila from contrasting light habitats. in Plant Species Biology. 2010;25(3):null-184.
https://hdl.handle.net/21.15107/rcub_ibiss_1352 .

Tucić, Branka, Miljković, Danijela, "Fluctuating asymmetry of floral organ traits in natural populations of Iris pumila from contrasting light habitats" in Plant Species Biology, 25, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1352 .

TY  - JOUR
AU  - Šešlija, Darka M.
AU  - Stojković, Biljana
AU  - Tucić, Branka
AU  - Tucić, Nikola G
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1131
AB  - In the present study the egg dumping behaviour in short (E)- and long (L)-lived lines of the seed beetle Acanthoscelides obtectus was analyzed. Females of the short-lived E line exhibited substantially higher egg dumping than long-lived L line females. We hypothesize that, since cessation of egg dumping enhances longevity, non-dumping females were selectively favoured in the L regime. Our study also produced evidence that the selection regime affected the male's ability to influence female egg-dumping behaviour. The females mated to males from the lines that were selected for extended longevity and of which the females exhibited little egg-dumping dumped fewer eggs. We suggest that in the L selection regime, where offspring produced at the end of the females' reproductive period were recruited to the next generation, selection operated against those males that stimulated female oviposition in the absence of seeds. This is the first study to provide evidence that selection for long-lived insects results in the reduced potency of male seminal products to stimulate female oviposition.
T2  - European Journal of Entomology
T1  - Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction
IS  - 4
VL  - 106
SP  - 255
EP  - 563
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1131
ER  - 

@article{
author = "Šešlija, Darka M. and Stojković, Biljana and Tucić, Branka and Tucić, Nikola G",
year = "2009",
abstract = "In the present study the egg dumping behaviour in short (E)- and long (L)-lived lines of the seed beetle Acanthoscelides obtectus was analyzed. Females of the short-lived E line exhibited substantially higher egg dumping than long-lived L line females. We hypothesize that, since cessation of egg dumping enhances longevity, non-dumping females were selectively favoured in the L regime. Our study also produced evidence that the selection regime affected the male's ability to influence female egg-dumping behaviour. The females mated to males from the lines that were selected for extended longevity and of which the females exhibited little egg-dumping dumped fewer eggs. We suggest that in the L selection regime, where offspring produced at the end of the females' reproductive period were recruited to the next generation, selection operated against those males that stimulated female oviposition in the absence of seeds. This is the first study to provide evidence that selection for long-lived insects results in the reduced potency of male seminal products to stimulate female oviposition.",
journal = "European Journal of Entomology",
title = "Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction",
number = "4",
volume = "106",
pages = "255-563",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1131"
}

Šešlija, D. M., Stojković, B., Tucić, B.,& Tucić, N. G.. (2009). Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction. in European Journal of Entomology, 106(4), 255-563.
https://hdl.handle.net/21.15107/rcub_ibiss_1131

Šešlija DM, Stojković B, Tucić B, Tucić NG. Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction. in European Journal of Entomology. 2009;106(4):255-563.
https://hdl.handle.net/21.15107/rcub_ibiss_1131 .

Šešlija, Darka M., Stojković, Biljana, Tucić, Branka, Tucić, Nikola G, "Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction" in European Journal of Entomology, 106, no. 4 (2009):255-563,
https://hdl.handle.net/21.15107/rcub_ibiss_1131 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Tucić, Branka
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/230
AB  - Thermal dependence of the enzymes SOD, CAT, and POD was investigated in leaves of Iris pumila plants inhabiting two contrasting light environments, a sun-exposed dune site and a woodland understory. At the same assay temperature, both the specific activity and the activation energy of SOD and CAT were higher in plants inhabiting vegetation shade than in those experiencing full sunlight. Conversely, the temperature optima for the two enzymes did not differ between alternative radiation environments. The specific activity of POD increased with temperature increase, and was always greater in plants growing under full sunlight than in those from vegetation shade. The activation energy of POD was higher than that of SOD or CAT, being lower in sun-than in shade-exposed plants.
AB  - U ovome radu ispitivana je temperaturna zavisnost enzima SOD, CAT i POD u listovima biljaka Iris pumila koje naseljavaju otvorena i zasenčena prirodna staništa. Utvrđeno je da su specifična aktivnost, kao i energija aktivacije SOD i CAT bile niže kod biljaka izloženih suncu nego kod onih koje naseljavaju senku, dok se temperaturni optimumi ovih enzima nisu razlikovali između staništa. Specifična aktivnost POD povećavala se sa porastom temperature, i bila je uvek viša kod biljaka izloženih suncu u poređenju sa biljkama iz senke. Aktivaciona energija enzima POD je bila viša od aktivacione energije SOD i CAT, ali relativno niža kod biljaka sa otvorenog nego sa zasenčenog staništa.
T2  - Archives of Biological Sciences
T1  - Temperaturna zavisnost antioksidacionih enzima superoksid dismutaze, katalaze i peroksidaze u listovima Iris pumila L.
T1  - Thermal dependence of the antioxidant enzymes superoxide dismutase, catalase, and peroxidase in foliage of Iris pumila L.
IS  - 3
VL  - 61
SP  - 441
EP  - 446
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_230
ER  - 

@article{
author = "Vuleta, Ana and Tucić, Branka",
year = "2009, 2009",
abstract = "Thermal dependence of the enzymes SOD, CAT, and POD was investigated in leaves of Iris pumila plants inhabiting two contrasting light environments, a sun-exposed dune site and a woodland understory. At the same assay temperature, both the specific activity and the activation energy of SOD and CAT were higher in plants inhabiting vegetation shade than in those experiencing full sunlight. Conversely, the temperature optima for the two enzymes did not differ between alternative radiation environments. The specific activity of POD increased with temperature increase, and was always greater in plants growing under full sunlight than in those from vegetation shade. The activation energy of POD was higher than that of SOD or CAT, being lower in sun-than in shade-exposed plants., U ovome radu ispitivana je temperaturna zavisnost enzima SOD, CAT i POD u listovima biljaka Iris pumila koje naseljavaju otvorena i zasenčena prirodna staništa. Utvrđeno je da su specifična aktivnost, kao i energija aktivacije SOD i CAT bile niže kod biljaka izloženih suncu nego kod onih koje naseljavaju senku, dok se temperaturni optimumi ovih enzima nisu razlikovali između staništa. Specifična aktivnost POD povećavala se sa porastom temperature, i bila je uvek viša kod biljaka izloženih suncu u poređenju sa biljkama iz senke. Aktivaciona energija enzima POD je bila viša od aktivacione energije SOD i CAT, ali relativno niža kod biljaka sa otvorenog nego sa zasenčenog staništa.",
journal = "Archives of Biological Sciences",
title = "Temperaturna zavisnost antioksidacionih enzima superoksid dismutaze, katalaze i peroksidaze u listovima Iris pumila L., Thermal dependence of the antioxidant enzymes superoxide dismutase, catalase, and peroxidase in foliage of Iris pumila L.",
number = "3",
volume = "61",
pages = "441-446",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_230"
}

Vuleta, A.,& Tucić, B.. (2009). Temperaturna zavisnost antioksidacionih enzima superoksid dismutaze, katalaze i peroksidaze u listovima Iris pumila L.. in Archives of Biological Sciences, 61(3), 441-446.
https://hdl.handle.net/21.15107/rcub_ibiss_230

Vuleta A, Tucić B. Temperaturna zavisnost antioksidacionih enzima superoksid dismutaze, katalaze i peroksidaze u listovima Iris pumila L.. in Archives of Biological Sciences. 2009;61(3):441-446.
https://hdl.handle.net/21.15107/rcub_ibiss_230 .

Vuleta, Ana, Tucić, Branka, "Temperaturna zavisnost antioksidacionih enzima superoksid dismutaze, katalaze i peroksidaze u listovima Iris pumila L." in Archives of Biological Sciences, 61, no. 3 (2009):441-446,
https://hdl.handle.net/21.15107/rcub_ibiss_230 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1490
AB  - Anthocyanins are a group of water-soluble flavonoids known for their protective role against photoinhibitory and photooxidative damage to leaf cells under environmental stress. The effects of variation in light quantity on rates of anthocyanin production in foliage of Iris pumila were evaluated spectrophotometrically in a field experimental setting accomplished by shielding one half of each examined plant with a 65% neutral-density shade, whereas the other half experienced full sunlight. In unshaded leaves, the average anthocyanin level increased by 55.3% compared to their shaded counterparts. Because there was no a significant difference in the average level of pheophytin (a breakdown product of chlorophyll) between unshaded and shaded leaves, the results suggested that the elevated anthocyanin concentrations in sun-exposed foliage of L pumila could act as a light attenuator, protecting its chloroplasts from excess high-energy quanta that would otherwise be intercepted by the chlorophylls.
T2  - Polish Journal of Ecology
T1  - Protective Function of Foliar Anthocyanins: in Situ Experiments on A Sun-Exposed Population of Iris Pumila L. (Iridaceae)
IS  - 4
VL  - 57
EP  - 783
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1490
ER  - 

@article{
author = "Tucić, Branka and Vuleta, Ana and Manitašević-Jovanović, Sanja",
year = "2009",
abstract = "Anthocyanins are a group of water-soluble flavonoids known for their protective role against photoinhibitory and photooxidative damage to leaf cells under environmental stress. The effects of variation in light quantity on rates of anthocyanin production in foliage of Iris pumila were evaluated spectrophotometrically in a field experimental setting accomplished by shielding one half of each examined plant with a 65% neutral-density shade, whereas the other half experienced full sunlight. In unshaded leaves, the average anthocyanin level increased by 55.3% compared to their shaded counterparts. Because there was no a significant difference in the average level of pheophytin (a breakdown product of chlorophyll) between unshaded and shaded leaves, the results suggested that the elevated anthocyanin concentrations in sun-exposed foliage of L pumila could act as a light attenuator, protecting its chloroplasts from excess high-energy quanta that would otherwise be intercepted by the chlorophylls.",
journal = "Polish Journal of Ecology",
title = "Protective Function of Foliar Anthocyanins: in Situ Experiments on A Sun-Exposed Population of Iris Pumila L. (Iridaceae)",
number = "4",
volume = "57",
pages = "783",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1490"
}

Tucić, B., Vuleta, A.,& Manitašević-Jovanović, S.. (2009). Protective Function of Foliar Anthocyanins: in Situ Experiments on A Sun-Exposed Population of Iris Pumila L. (Iridaceae). in Polish Journal of Ecology, 57(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1490

Tucić B, Vuleta A, Manitašević-Jovanović S. Protective Function of Foliar Anthocyanins: in Situ Experiments on A Sun-Exposed Population of Iris Pumila L. (Iridaceae). in Polish Journal of Ecology. 2009;57(4):null-783.
https://hdl.handle.net/21.15107/rcub_ibiss_1490 .

Tucić, Branka, Vuleta, Ana, Manitašević-Jovanović, Sanja, "Protective Function of Foliar Anthocyanins: in Situ Experiments on A Sun-Exposed Population of Iris Pumila L. (Iridaceae)" in Polish Journal of Ecology, 57, no. 4 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1490 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Manitašević, Sanja
AU  - Vuleta, Ana
AU  - Matić, Gordana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/201
AB  - Hsp90 is an environmentally responsive molecular chaperone that was found to play a key role in buffering against genetic and non-genetic perturbations in the model organisms Arabidopsis and Drosophila. Here we analyzed the buffering capacity of Hsp90 against two kinds of non-genetic factors - stochastic noise and micro-environmental varia­tion of floral organ traits in naturally growing Iris pumila plants. We found no statistical association between the endog­enous level of Hsp90 and the floral organ radial symmetry produced by stochastic developmental noise. Conversely, floral organ plasticity in response to micro-environmental variation tended to be greater with decrease in Hsp90b isoform expression.
AB  - Molekularni šaperon Hsp90 ima ključnu ulogu u neutralisanju genetičkih i negenetičkih poremećaja kod model organizama Arabidopsis i Drosophila. U ovom radu analizirali smo zaštitni kapacitet Hsp90 u odnosu na dve vrste negenetičkih faktora - stohastičko i mikro-sredinsko variranje osobina cveta u prirodnoj populaciji Iris pumila. Utvrdili smo da nema statistički značajne korelacije između endogenog nivoa proteina Hsp90 i radijalne asimetrije uslovljene slučajnim promenama u toku razvića analiziranih cvetnih organa. Nasuprot tome, plastičnost cvetnih organa u odnosu na mikro-sredinsko variranje povećavala se sa smanjenjem ekspresije izoforme Hsp90b.
T2  - Archives of Biological Sciences
T1  - Povezanost funkcije Hsp90 sa mikro-sredinskim i slučajnim variranjem cvetnih organa Iris pumila L.
T1  - Linking Hsp90 function to micro-environmental and stochastic variation in floralorgans of Iris pumila L.
IS  - 3
VL  - 60
DO  - 10.2298/ABS0803411T
SP  - 411
EP  - 419
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_201
ER  - 

@article{
author = "Tucić, Branka and Manitašević, Sanja and Vuleta, Ana and Matić, Gordana",
year = "2008",
abstract = "Hsp90 is an environmentally responsive molecular chaperone that was found to play a key role in buffering against genetic and non-genetic perturbations in the model organisms Arabidopsis and Drosophila. Here we analyzed the buffering capacity of Hsp90 against two kinds of non-genetic factors - stochastic noise and micro-environmental varia­tion of floral organ traits in naturally growing Iris pumila plants. We found no statistical association between the endog­enous level of Hsp90 and the floral organ radial symmetry produced by stochastic developmental noise. Conversely, floral organ plasticity in response to micro-environmental variation tended to be greater with decrease in Hsp90b isoform expression., Molekularni šaperon Hsp90 ima ključnu ulogu u neutralisanju genetičkih i negenetičkih poremećaja kod model organizama Arabidopsis i Drosophila. U ovom radu analizirali smo zaštitni kapacitet Hsp90 u odnosu na dve vrste negenetičkih faktora - stohastičko i mikro-sredinsko variranje osobina cveta u prirodnoj populaciji Iris pumila. Utvrdili smo da nema statistički značajne korelacije između endogenog nivoa proteina Hsp90 i radijalne asimetrije uslovljene slučajnim promenama u toku razvića analiziranih cvetnih organa. Nasuprot tome, plastičnost cvetnih organa u odnosu na mikro-sredinsko variranje povećavala se sa smanjenjem ekspresije izoforme Hsp90b.",
journal = "Archives of Biological Sciences",
title = "Povezanost funkcije Hsp90 sa mikro-sredinskim i slučajnim variranjem cvetnih organa Iris pumila L., Linking Hsp90 function to micro-environmental and stochastic variation in floralorgans of Iris pumila L.",
number = "3",
volume = "60",
doi = "10.2298/ABS0803411T",
pages = "411-419",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_201"
}

Tucić, B., Manitašević, S., Vuleta, A.,& Matić, G.. (2008). Povezanost funkcije Hsp90 sa mikro-sredinskim i slučajnim variranjem cvetnih organa Iris pumila L.. in Archives of Biological Sciences, 60(3), 411-419.
https://doi.org/10.2298/ABS0803411T
https://hdl.handle.net/21.15107/rcub_ibiss_201

Tucić B, Manitašević S, Vuleta A, Matić G. Povezanost funkcije Hsp90 sa mikro-sredinskim i slučajnim variranjem cvetnih organa Iris pumila L.. in Archives of Biological Sciences. 2008;60(3):411-419.
doi:10.2298/ABS0803411T
https://hdl.handle.net/21.15107/rcub_ibiss_201 .

Tucić, Branka, Manitašević, Sanja, Vuleta, Ana, Matić, Gordana, "Povezanost funkcije Hsp90 sa mikro-sredinskim i slučajnim variranjem cvetnih organa Iris pumila L." in Archives of Biological Sciences, 60, no. 3 (2008):411-419,
https://doi.org/10.2298/ABS0803411T .,
https://hdl.handle.net/21.15107/rcub_ibiss_201 .

TY  - JOUR
AU  - Manitašević, Sanja
AU  - Dunđerski, Jadranka S.
AU  - Matić, Gordana
AU  - Tucić, Branka
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1621
AB  - Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression was studied in the leaves of two naturally growing Iris pumila populations, one inhabiting an open dune site, and the other the understorey of a Pinus silvestris stand. The Hsps were quantified by an immunoblotting procedure. The level of the Hsps was found to vary significantly both across seasons and between habitats. The mean Hsp70 concentration was significantly greater at the open area than in the woodland understorey, reaching its maximum in the summer, especially in plants experiencing full sunlight. Two Hsp90 isoforms, referred to as Hsp90a (86 kDa) and Hsp90b (84 kDa), were detected. At both habitats, the level of Hsp90a was highest in autumn, that of Hsp90b in spring, whereas both of them reached a nadir in summer. Throughout the growing season, the relative abundance of Hsp90b was higher in plants growing under vegetation canopy in comparison to those inhabiting the open dune site. An inverse relationship between the phenotypic variation in specific leaf area and the level of Hsp90b over seasons at both habitats was observed, suggesting the role of this protein in buffering phenotypic variation in the wild.
T2  - Plant Cell and Environment
T1  - Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression in an exposed and a shaded habitat of Iris pumila
IS  - 1
VL  - 30
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1621
ER  - 

@article{
author = "Manitašević, Sanja and Dunđerski, Jadranka S. and Matić, Gordana and Tucić, Branka",
year = "2007",
abstract = "Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression was studied in the leaves of two naturally growing Iris pumila populations, one inhabiting an open dune site, and the other the understorey of a Pinus silvestris stand. The Hsps were quantified by an immunoblotting procedure. The level of the Hsps was found to vary significantly both across seasons and between habitats. The mean Hsp70 concentration was significantly greater at the open area than in the woodland understorey, reaching its maximum in the summer, especially in plants experiencing full sunlight. Two Hsp90 isoforms, referred to as Hsp90a (86 kDa) and Hsp90b (84 kDa), were detected. At both habitats, the level of Hsp90a was highest in autumn, that of Hsp90b in spring, whereas both of them reached a nadir in summer. Throughout the growing season, the relative abundance of Hsp90b was higher in plants growing under vegetation canopy in comparison to those inhabiting the open dune site. An inverse relationship between the phenotypic variation in specific leaf area and the level of Hsp90b over seasons at both habitats was observed, suggesting the role of this protein in buffering phenotypic variation in the wild.",
journal = "Plant Cell and Environment",
title = "Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression in an exposed and a shaded habitat of Iris pumila",
number = "1",
volume = "30",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1621"
}

Manitašević, S., Dunđerski, J. S., Matić, G.,& Tucić, B.. (2007). Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression in an exposed and a shaded habitat of Iris pumila. in Plant Cell and Environment, 30(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1621

Manitašević S, Dunđerski JS, Matić G, Tucić B. Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression in an exposed and a shaded habitat of Iris pumila. in Plant Cell and Environment. 2007;30(1):null-11.
https://hdl.handle.net/21.15107/rcub_ibiss_1621 .

Manitašević, Sanja, Dunđerski, Jadranka S., Matić, Gordana, Tucić, Branka, "Seasonal variation in heat shock proteins Hsp70 and Hsp90 expression in an exposed and a shaded habitat of Iris pumila" in Plant Cell and Environment, 30, no. 1 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1621 .