TY  - CONF
AU  - Sokanović, Srđan
AU  - Constantin, Stephanie
AU  - Lamarca Dams, Aloa
AU  - Mochimaru, Yuta
AU  - Smiljanić, Kosara
AU  - Bjelobaba, Ivana
AU  - Prévide, Rafael
AU  - Stojilković, Stanko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5854
AB  - Simultaneous knockout of the neuroendocrine marker genes Ptprn and Ptprn2, which 
encode the protein tyrosine phosphatase receptors N and N2, respectively, causes 
infertility of female mice while males are fertile. To clarify the mechanism of sex specific roles of Ptprn and Ptprn2 in mice reproduction, we analyzed the effects of 
their double knockout (DKO) on the hypothalamic-pituitary-gonadal axis. In DKO 
females, a delay in puberty and lack of ovulation were observed, supplemented by 
changes in ovarian gene expression and steroidogenesis. In DKO males, the testicular 
gene expression, steroidogenesis, and development of reproductive organs were not 
affected. However, in both sexes, pituitary luteinizing hormone (LH) beta gene
expression and LH levels were reduced, while the calcium-mobilizing and LH 
secretory actions of gonadotropin-releasing hormone (GnRH) receptors were 
preserved. The expression of hypothalamic Gnrh1 and Kiss1 genes were also reduced 
in DKO females and males. The density of immunoreactive GnRH fibers was 
decreased in the median eminence in DKO females and males. The density of 
immunoreactive kisspeptin fibers was also decreased in the rostral periventricular 
region of the third ventricle of females and in the arcuate nucleus of females and 
males. Therefore, infertility in DKO females cannot be explained only by sex-specific 
gonadotroph impairment. Instead, changes in hypothalamic gene expression, 
specifically Kiss1 in the rostral periventricular region of the third ventricle, might 
provide an alternative hypothesis due to its sexual dimorphism and involvement in 
puberty onset and ovulation.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Protein tyrosine phosphatase receptors N and N2 regulate  gonadotropin-releasing hormone neuron function
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5854
ER  - 

@conference{
author = "Sokanović, Srđan and Constantin, Stephanie and Lamarca Dams, Aloa and Mochimaru, Yuta and Smiljanić, Kosara and Bjelobaba, Ivana and Prévide, Rafael and Stojilković, Stanko",
year = "2023",
abstract = "Simultaneous knockout of the neuroendocrine marker genes Ptprn and Ptprn2, which 
encode the protein tyrosine phosphatase receptors N and N2, respectively, causes 
infertility of female mice while males are fertile. To clarify the mechanism of sex specific roles of Ptprn and Ptprn2 in mice reproduction, we analyzed the effects of 
their double knockout (DKO) on the hypothalamic-pituitary-gonadal axis. In DKO 
females, a delay in puberty and lack of ovulation were observed, supplemented by 
changes in ovarian gene expression and steroidogenesis. In DKO males, the testicular 
gene expression, steroidogenesis, and development of reproductive organs were not 
affected. However, in both sexes, pituitary luteinizing hormone (LH) beta gene
expression and LH levels were reduced, while the calcium-mobilizing and LH 
secretory actions of gonadotropin-releasing hormone (GnRH) receptors were 
preserved. The expression of hypothalamic Gnrh1 and Kiss1 genes were also reduced 
in DKO females and males. The density of immunoreactive GnRH fibers was 
decreased in the median eminence in DKO females and males. The density of 
immunoreactive kisspeptin fibers was also decreased in the rostral periventricular 
region of the third ventricle of females and in the arcuate nucleus of females and 
males. Therefore, infertility in DKO females cannot be explained only by sex-specific 
gonadotroph impairment. Instead, changes in hypothalamic gene expression, 
specifically Kiss1 in the rostral periventricular region of the third ventricle, might 
provide an alternative hypothesis due to its sexual dimorphism and involvement in 
puberty onset and ovulation.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Protein tyrosine phosphatase receptors N and N2 regulate  gonadotropin-releasing hormone neuron function",
pages = "107",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5854"
}

Sokanović, S., Constantin, S., Lamarca Dams, A., Mochimaru, Y., Smiljanić, K., Bjelobaba, I., Prévide, R.,& Stojilković, S.. (2023). Protein tyrosine phosphatase receptors N and N2 regulate  gonadotropin-releasing hormone neuron function. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 107.
https://hdl.handle.net/21.15107/rcub_ibiss_5854

Sokanović S, Constantin S, Lamarca Dams A, Mochimaru Y, Smiljanić K, Bjelobaba I, Prévide R, Stojilković S. Protein tyrosine phosphatase receptors N and N2 regulate  gonadotropin-releasing hormone neuron function. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:107.
https://hdl.handle.net/21.15107/rcub_ibiss_5854 .

Sokanović, Srđan, Constantin, Stephanie, Lamarca Dams, Aloa, Mochimaru, Yuta, Smiljanić, Kosara, Bjelobaba, Ivana, Prévide, Rafael, Stojilković, Stanko, "Protein tyrosine phosphatase receptors N and N2 regulate  gonadotropin-releasing hormone neuron function" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):107,
https://hdl.handle.net/21.15107/rcub_ibiss_5854 .