TY  - CONF
AU  - Ghasemian, Ehsan
AU  - Stein, Elisabeth
AU  - Inić-Kanada, Aleksandra
AU  - Schuerer, Nadine
AU  - Bintner, Nora
AU  - Rajić, Jovana
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - https://iovs.arvojournals.org/article.aspx?articleid=2642535
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6313
AB  - Purpose : Bacterial ghosts (BGs) are empty cell envelopes derived from Gram-negative bacteria. BGs possess all cell membrane structures, like live bacteria they are originated from, which gives a potential to use them as an adjuvant delivery system. In the present study, we investigated the potential of Escherichia coli Nissle (EcN) and Salmonella typhimurium (Sty) BGs in modulation of immune responses in human conjunctival epithelial cells (HCjE).
Methods : Confluent monolayers of HCjE cells were treated for 2 and 24 hours with EcN and Sty BGs at a concentration of 1x108 particles/ml, as well as with Interleukin- 1α as a general stimulator. 24 hours after cell stimulation the supernatants and cells were collected. Concentration of IL-6 and IL-8 were measured using ELISA. Quantification of the gene expression levels for TLR-4 and TLR-5 was performed using qRT-PCR.
Results : HCjE cells responded to EcN BGs and Sty BGs with increased secretion of pro-inflammatory cytokines and expression of toll-like receptors (TLRs). Expression of TLR-4 and TLR-5 was significantly higher in EcN BGs stimulated cells compared to Sty BGs stimulated HCjE cells (P<0.05). EcN and Sty BGs induced enhanced secretion of IL-6 and IL-8 in HCjE cells.
Conclusions : Our results suggest that both used BGs may modulate the secretion of IL-6 and IL-8 mainly via the activation of TLR-4 and TLR-5 in HCjE cells.
PB  - Rockville: Association for Research in Vision and Ophthalmolog Inc.
C3  - Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA
T1  - The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts on human conjunctival epithelial cells
SP  - 5773
EP  - 5773
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6313
ER  - 

@conference{
author = "Ghasemian, Ehsan and Stein, Elisabeth and Inić-Kanada, Aleksandra and Schuerer, Nadine and Bintner, Nora and Rajić, Jovana and Barisani-Asenbauer, Talin",
year = "2017",
abstract = "Purpose : Bacterial ghosts (BGs) are empty cell envelopes derived from Gram-negative bacteria. BGs possess all cell membrane structures, like live bacteria they are originated from, which gives a potential to use them as an adjuvant delivery system. In the present study, we investigated the potential of Escherichia coli Nissle (EcN) and Salmonella typhimurium (Sty) BGs in modulation of immune responses in human conjunctival epithelial cells (HCjE).
Methods : Confluent monolayers of HCjE cells were treated for 2 and 24 hours with EcN and Sty BGs at a concentration of 1x108 particles/ml, as well as with Interleukin- 1α as a general stimulator. 24 hours after cell stimulation the supernatants and cells were collected. Concentration of IL-6 and IL-8 were measured using ELISA. Quantification of the gene expression levels for TLR-4 and TLR-5 was performed using qRT-PCR.
Results : HCjE cells responded to EcN BGs and Sty BGs with increased secretion of pro-inflammatory cytokines and expression of toll-like receptors (TLRs). Expression of TLR-4 and TLR-5 was significantly higher in EcN BGs stimulated cells compared to Sty BGs stimulated HCjE cells (P<0.05). EcN and Sty BGs induced enhanced secretion of IL-6 and IL-8 in HCjE cells.
Conclusions : Our results suggest that both used BGs may modulate the secretion of IL-6 and IL-8 mainly via the activation of TLR-4 and TLR-5 in HCjE cells.",
publisher = "Rockville: Association for Research in Vision and Ophthalmolog Inc.",
journal = "Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA",
title = "The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts on human conjunctival epithelial cells",
pages = "5773-5773",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6313"
}

Ghasemian, E., Stein, E., Inić-Kanada, A., Schuerer, N., Bintner, N., Rajić, J.,& Barisani-Asenbauer, T.. (2017). The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts on human conjunctival epithelial cells. in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA
Rockville: Association for Research in Vision and Ophthalmolog Inc.., 5773-5773.
https://hdl.handle.net/21.15107/rcub_ibiss_6313

Ghasemian E, Stein E, Inić-Kanada A, Schuerer N, Bintner N, Rajić J, Barisani-Asenbauer T. The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts on human conjunctival epithelial cells. in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA. 2017;:5773-5773.
https://hdl.handle.net/21.15107/rcub_ibiss_6313 .

Ghasemian, Ehsan, Stein, Elisabeth, Inić-Kanada, Aleksandra, Schuerer, Nadine, Bintner, Nora, Rajić, Jovana, Barisani-Asenbauer, Talin, "The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts on human conjunctival epithelial cells" in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA (2017):5773-5773,
https://hdl.handle.net/21.15107/rcub_ibiss_6313 .

TY  - CONF
AU  - Barisani-Asenbauer, Talin
AU  - Inić-Kanada, Aleksandra
AU  - Rajić, Jovana
AU  - Grdović, Nevena
AU  - Stein, Elisabeth
AU  - Ghasemian, Ehsan
AU  - Schuerer, Nadine
AU  - Vidaković, Melita
PY  - 2017
UR  - https://iovs.arvojournals.org/article.aspx?articleid=2642540
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6314
AB  - Purpose : Epithelial-mesenchymal transition (EMT) of conjunctival epithelium may contribute to trachoma-associated conjunctival fibrosis. Epigenetic mechanisms have an important role in different disease etiologies, however, it is unknown whether they play a role during Chlamydia trachomatis (Ct) ocular infection. The aim of this study was to test the ability of Ct to induce EMT in human conjunctival epithelial (HCjE) cells in vitro and investigate whether alterations in gene expression after Ct infection are correlated with DNA methylation of EMT related marker genes.
Methods : mRNA and protein expression levels of EMT markers, including TGFβ1 and TGFβ2, E-cadherin (CDH1), fibronectin (FN1) and α-smooth muscle actin (ACTA2) were assayed by qPCR and Western blot analysis in HCjE cells infected with Ct serovar B. Promoters and gene-bodies of CDH1, FN1 and ACTA2 were analyzed for the presence of CpG islands using CpG Island Searcher software and thereafter changes in DNA methylation patterns of selected regions were examined with MSP, HRM and bisulfite sequencing.
Results : Infection of HCjE cells with Ct resulted in a statistically significant increase in TGFβ1 and TGFβ2 mRNA and significant reduction of mRNA expression of E-cadherin gene (CDH1), wherein CDH1 mRNA expression was 2.7-fold higher in a control than in Ct infected HCjE cells. Decrease in mRNA expression was accompanied with diminishment in protein level of E-cadherin after Ct infection. HRM data showed convincing difference between control and Ct infected level of DNA methylation with increase from 12.8% to 21.8% suggesting that CDH1 promoter region is subjected to alterations in DNA methylation in HCjE cells because of Ct infection.
Conclusions : Our data for the first time indicate a direct role of Ct infection in epigenetic regulation of human conjunctival epithelium. Studying the pathways of EMT in HCjE cells may help to develop new therapeutic strategies to treat conjunctival fibrosis.
PB  - Rockville: Association for Research in Vision and Ophthalmolog Inc.
C3  - Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA
T1  - Chlamydia trachomatis infection induces epithelial-mesenchymal transition in conjunctival epithelial cells
SP  - 5778
EP  - 5778
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6314
ER  - 

@conference{
author = "Barisani-Asenbauer, Talin and Inić-Kanada, Aleksandra and Rajić, Jovana and Grdović, Nevena and Stein, Elisabeth and Ghasemian, Ehsan and Schuerer, Nadine and Vidaković, Melita",
year = "2017",
abstract = "Purpose : Epithelial-mesenchymal transition (EMT) of conjunctival epithelium may contribute to trachoma-associated conjunctival fibrosis. Epigenetic mechanisms have an important role in different disease etiologies, however, it is unknown whether they play a role during Chlamydia trachomatis (Ct) ocular infection. The aim of this study was to test the ability of Ct to induce EMT in human conjunctival epithelial (HCjE) cells in vitro and investigate whether alterations in gene expression after Ct infection are correlated with DNA methylation of EMT related marker genes.
Methods : mRNA and protein expression levels of EMT markers, including TGFβ1 and TGFβ2, E-cadherin (CDH1), fibronectin (FN1) and α-smooth muscle actin (ACTA2) were assayed by qPCR and Western blot analysis in HCjE cells infected with Ct serovar B. Promoters and gene-bodies of CDH1, FN1 and ACTA2 were analyzed for the presence of CpG islands using CpG Island Searcher software and thereafter changes in DNA methylation patterns of selected regions were examined with MSP, HRM and bisulfite sequencing.
Results : Infection of HCjE cells with Ct resulted in a statistically significant increase in TGFβ1 and TGFβ2 mRNA and significant reduction of mRNA expression of E-cadherin gene (CDH1), wherein CDH1 mRNA expression was 2.7-fold higher in a control than in Ct infected HCjE cells. Decrease in mRNA expression was accompanied with diminishment in protein level of E-cadherin after Ct infection. HRM data showed convincing difference between control and Ct infected level of DNA methylation with increase from 12.8% to 21.8% suggesting that CDH1 promoter region is subjected to alterations in DNA methylation in HCjE cells because of Ct infection.
Conclusions : Our data for the first time indicate a direct role of Ct infection in epigenetic regulation of human conjunctival epithelium. Studying the pathways of EMT in HCjE cells may help to develop new therapeutic strategies to treat conjunctival fibrosis.",
publisher = "Rockville: Association for Research in Vision and Ophthalmolog Inc.",
journal = "Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA",
title = "Chlamydia trachomatis infection induces epithelial-mesenchymal transition in conjunctival epithelial cells",
pages = "5778-5778",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6314"
}

Barisani-Asenbauer, T., Inić-Kanada, A., Rajić, J., Grdović, N., Stein, E., Ghasemian, E., Schuerer, N.,& Vidaković, M.. (2017). Chlamydia trachomatis infection induces epithelial-mesenchymal transition in conjunctival epithelial cells. in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA
Rockville: Association for Research in Vision and Ophthalmolog Inc.., 5778-5778.
https://hdl.handle.net/21.15107/rcub_ibiss_6314

Barisani-Asenbauer T, Inić-Kanada A, Rajić J, Grdović N, Stein E, Ghasemian E, Schuerer N, Vidaković M. Chlamydia trachomatis infection induces epithelial-mesenchymal transition in conjunctival epithelial cells. in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA. 2017;:5778-5778.
https://hdl.handle.net/21.15107/rcub_ibiss_6314 .

Barisani-Asenbauer, Talin, Inić-Kanada, Aleksandra, Rajić, Jovana, Grdović, Nevena, Stein, Elisabeth, Ghasemian, Ehsan, Schuerer, Nadine, Vidaković, Melita, "Chlamydia trachomatis infection induces epithelial-mesenchymal transition in conjunctival epithelial cells" in Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO); 2017 May 7-11; Baltimore, USA (2017):5778-5778,
https://hdl.handle.net/21.15107/rcub_ibiss_6314 .

TY  - CONF
AU  - Rajić, Jovana
AU  - Grdović, Nevena
AU  - Inic-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Schuerer, Nadine
AU  - Uskoković, Aleksandra
AU  - Ghasemian, Ehsan
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Tolić, Anja
AU  - Đorđević, Marija
AU  - Đorđević, Miloš
AU  - Poznanović, Goran
AU  - Barisani-Asenbauer, Talin
AU  - Vidaković, Melita
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2866
UR  - http://molbios.bio.bg.ac.rs/combos
AB  - Trachoma is the leading infectious cause of blindness worldwide initiated by repeated infections of the conjunctiva with Chlamydia trachomatis (Ct). Consequent chronic inflammation results in formation of fibrotic tissue which can eventually lead to damage of the corneal surface. Lately, emerging evidence suggests involvement of epithelial to mesenchymaltransition (EMT) in development of every fibrotic process. Process of EMT represents reversible transition of epithelial into mesenchymalcells that can be traced through numerous markers such as E-cadherin, highly expressed in epithelial cells, α-SMA, a frequently used mesenchymalEMT marker and fibronectin, a glycoprotein whose expression increases during the EMT process. Recent findings have suggested that epigenetic events are master regulators of expression of many EMT related genes.
C3  - 1st Congress of Molecular Biologists of Serbia
T1  - Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva: possible epigenetic mechanisms unveiled
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2866
ER  - 

@conference{
author = "Rajić, Jovana and Grdović, Nevena and Inic-Kanada, Aleksandra and Stein, Elisabeth and Schuerer, Nadine and Uskoković, Aleksandra and Ghasemian, Ehsan and Dinić, Svetlana and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Tolić, Anja and Đorđević, Marija and Đorđević, Miloš and Poznanović, Goran and Barisani-Asenbauer, Talin and Vidaković, Melita",
year = "2017",
abstract = "Trachoma is the leading infectious cause of blindness worldwide initiated by repeated infections of the conjunctiva with Chlamydia trachomatis (Ct). Consequent chronic inflammation results in formation of fibrotic tissue which can eventually lead to damage of the corneal surface. Lately, emerging evidence suggests involvement of epithelial to mesenchymaltransition (EMT) in development of every fibrotic process. Process of EMT represents reversible transition of epithelial into mesenchymalcells that can be traced through numerous markers such as E-cadherin, highly expressed in epithelial cells, α-SMA, a frequently used mesenchymalEMT marker and fibronectin, a glycoprotein whose expression increases during the EMT process. Recent findings have suggested that epigenetic events are master regulators of expression of many EMT related genes.",
journal = "1st Congress of Molecular Biologists of Serbia",
title = "Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva: possible epigenetic mechanisms unveiled",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2866"
}

Rajić, J., Grdović, N., Inic-Kanada, A., Stein, E., Schuerer, N., Uskoković, A., Ghasemian, E., Dinić, S., Mihailović, M., Arambašić Jovanović, J., Tolić, A., Đorđević, M., Đorđević, M., Poznanović, G., Barisani-Asenbauer, T.,& Vidaković, M.. (2017). Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva: possible epigenetic mechanisms unveiled. in 1st Congress of Molecular Biologists of Serbia.
https://hdl.handle.net/21.15107/rcub_ibiss_2866

Rajić J, Grdović N, Inic-Kanada A, Stein E, Schuerer N, Uskoković A, Ghasemian E, Dinić S, Mihailović M, Arambašić Jovanović J, Tolić A, Đorđević M, Đorđević M, Poznanović G, Barisani-Asenbauer T, Vidaković M. Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva: possible epigenetic mechanisms unveiled. in 1st Congress of Molecular Biologists of Serbia. 2017;.
https://hdl.handle.net/21.15107/rcub_ibiss_2866 .

Rajić, Jovana, Grdović, Nevena, Inic-Kanada, Aleksandra, Stein, Elisabeth, Schuerer, Nadine, Uskoković, Aleksandra, Ghasemian, Ehsan, Dinić, Svetlana, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Tolić, Anja, Đorđević, Marija, Đorđević, Miloš, Poznanović, Goran, Barisani-Asenbauer, Talin, Vidaković, Melita, "Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva: possible epigenetic mechanisms unveiled" in 1st Congress of Molecular Biologists of Serbia (2017),
https://hdl.handle.net/21.15107/rcub_ibiss_2866 .

TY  - CONF
AU  - Rajić, Jovana
AU  - Grdović, Nevena
AU  - Inic-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Schuerer, Nadine
AU  - Uskoković, Aleksandra
AU  - Ghasemian, Ehsan
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Tolić, Anja
AU  - Đorđević, Marija
AU  - Đorđević, Miloš
AU  - Poznanović, Goran
AU  - Barisani-Asenbauer, Talin
AU  - Vidaković, Melita
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2869
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2866
AB  - Introduction: Trachoma is the most common cause of infectious blindness worldwide, initiated by repeated infection of the conjunctiva with Chlamydia trachomatis (Ct). The resulting chronic inflammation and formation of fibrotic tissue eventually lead to corneal damage. Based on the facts that epithelial to mesenchymal transition (EMT) plays an important role in the development of fibrosis and that EMT is epigenetically regulated process, the aims of this study were to reveal the capacity of Ct to induce EMT in vitro and to unveil potential underlying epigenetic mechanisms. Methods: Human conjunctival epithelial (HCjE) cells were infected with 107 IFU of Ct for 72 h. EMT-inducing signaling pathways, as well as mRNA and protein expression of EMT markers (E-cadherin, fibronectin and α-SMA) were evaluated by RT-qPCR, Immunoblotting and Immunocytochemistry. DNA methylation patterns of selected regions of E-cadherin, fibronectin and α-SMA genes were examined by Methylation-Specific PCR, High Resolution Melting analysis and Bisulfite Sequencing. Results: Infection with Ct was accompanied with the activation of EMT-inducing signaling pathways, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal markers fibronectin and α-SMA. While DNA methylation status of E-cadherin gene promoter correlated with its expression, methylation status of fibronectin and α-SMA genes couldn’t be related to their expression levels. Conclusion: Ct infection of HCjE cells triggers EMT that goes along with changes in the methylation profile of the E-cadherin promoter. Sequence of events described herein could contribute to scarring process in trachoma and open up possibilities for development of new therapeutic strategies in trachoma treatment.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - 1st Congress of Molecular Biologists of Serbia
T1  - Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2869
ER  - 

@conference{
editor = "Brajušković, Goran, Đorđević, Ana",
author = "Rajić, Jovana and Grdović, Nevena and Inic-Kanada, Aleksandra and Stein, Elisabeth and Schuerer, Nadine and Uskoković, Aleksandra and Ghasemian, Ehsan and Dinić, Svetlana and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Tolić, Anja and Đorđević, Marija and Đorđević, Miloš and Poznanović, Goran and Barisani-Asenbauer, Talin and Vidaković, Melita",
year = "2017",
abstract = "Introduction: Trachoma is the most common cause of infectious blindness worldwide, initiated by repeated infection of the conjunctiva with Chlamydia trachomatis (Ct). The resulting chronic inflammation and formation of fibrotic tissue eventually lead to corneal damage. Based on the facts that epithelial to mesenchymal transition (EMT) plays an important role in the development of fibrosis and that EMT is epigenetically regulated process, the aims of this study were to reveal the capacity of Ct to induce EMT in vitro and to unveil potential underlying epigenetic mechanisms. Methods: Human conjunctival epithelial (HCjE) cells were infected with 107 IFU of Ct for 72 h. EMT-inducing signaling pathways, as well as mRNA and protein expression of EMT markers (E-cadherin, fibronectin and α-SMA) were evaluated by RT-qPCR, Immunoblotting and Immunocytochemistry. DNA methylation patterns of selected regions of E-cadherin, fibronectin and α-SMA genes were examined by Methylation-Specific PCR, High Resolution Melting analysis and Bisulfite Sequencing. Results: Infection with Ct was accompanied with the activation of EMT-inducing signaling pathways, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal markers fibronectin and α-SMA. While DNA methylation status of E-cadherin gene promoter correlated with its expression, methylation status of fibronectin and α-SMA genes couldn’t be related to their expression levels. Conclusion: Ct infection of HCjE cells triggers EMT that goes along with changes in the methylation profile of the E-cadherin promoter. Sequence of events described herein could contribute to scarring process in trachoma and open up possibilities for development of new therapeutic strategies in trachoma treatment.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "1st Congress of Molecular Biologists of Serbia",
title = "Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2869"
}

Brajušković, G., Đorđević, A., Rajić, J., Grdović, N., Inic-Kanada, A., Stein, E., Schuerer, N., Uskoković, A., Ghasemian, E., Dinić, S., Mihailović, M., Arambašić Jovanović, J., Tolić, A., Đorđević, M., Đorđević, M., Poznanović, G., Barisani-Asenbauer, T.,& Vidaković, M.. (2017). Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled. in 1st Congress of Molecular Biologists of Serbia
Belgrade: University of Belgrade, Faculty of Biology., 70-70.
https://hdl.handle.net/21.15107/rcub_ibiss_2869

Brajušković G, Đorđević A, Rajić J, Grdović N, Inic-Kanada A, Stein E, Schuerer N, Uskoković A, Ghasemian E, Dinić S, Mihailović M, Arambašić Jovanović J, Tolić A, Đorđević M, Đorđević M, Poznanović G, Barisani-Asenbauer T, Vidaković M. Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled. in 1st Congress of Molecular Biologists of Serbia. 2017;:70-70.
https://hdl.handle.net/21.15107/rcub_ibiss_2869 .

Brajušković, Goran, Đorđević, Ana, Rajić, Jovana, Grdović, Nevena, Inic-Kanada, Aleksandra, Stein, Elisabeth, Schuerer, Nadine, Uskoković, Aleksandra, Ghasemian, Ehsan, Dinić, Svetlana, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Tolić, Anja, Đorđević, Marija, Đorđević, Miloš, Poznanović, Goran, Barisani-Asenbauer, Talin, Vidaković, Melita, "Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled" in 1st Congress of Molecular Biologists of Serbia (2017):70-70,
https://hdl.handle.net/21.15107/rcub_ibiss_2869 .

TY  - JOUR
AU  - Rajić, Jovana
AU  - Inic-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Dinić, Svetlana
AU  - Schuerer, Nadine
AU  - Uskoković, Aleksandra
AU  - Ghasemian, Ehsan
AU  - Mihailović, Mirjana
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://journal.frontiersin.org/article/10.3389/fcimb.2017.00253/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2836
AB  - Chlamydia trachomatis (Ct) can induce scarring disease of the ocular mucosa, known as trachoma, the most common infectious cause of blindness worldwide. We hypothesized that epithelial-mesenchymal transition (EMT) contributes to the fibrotic process in trachomatous scarring. Infection of human conjunctival epithelial cells (HCjE) with Ct activated signaling pathways involved in EMT induction, which was correlated with decreased expression of E-cadherin, guardian of the epithelial phenotype. In addition, Ct infection was associated with increased expression of two mesenchymal cell markers: fibronectin and α-SMA. The DNA methylation statuses of selected regions of E-cadherin, fibronectin, and α-SMA genes revealed that Ct infection was accompanied with changes in DNA methylation of the E-cadherin promoter, while the expression of the two mesenchymal markers was not related with this epigenetic event. Our data suggest that Ct infection of conjunctival epithelial cells induces EMT-like changes that go along with modification of the methylation profile of the E-cadherin promoter and could, as one of the earliest events, contribute to processes triggering conjunctival scarring.
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Chlamydia trachomatis Infection Is Associated with E-Cadherin Promoter Methylation, Downregulation of E-Cadherin Expression, and Increased Expression of Fibronectin and α-SMA—Implications for Epithelial-Mesenchymal Transition
IS  - JUN
VL  - 7
DO  - 10.3389/fcimb.2017.00253
SP  - 253
EP  - 253
ER  - 

@article{
author = "Rajić, Jovana and Inic-Kanada, Aleksandra and Stein, Elisabeth and Dinić, Svetlana and Schuerer, Nadine and Uskoković, Aleksandra and Ghasemian, Ehsan and Mihailović, Mirjana and Vidaković, Melita and Grdović, Nevena and Barisani-Asenbauer, Talin",
year = "2017",
abstract = "Chlamydia trachomatis (Ct) can induce scarring disease of the ocular mucosa, known as trachoma, the most common infectious cause of blindness worldwide. We hypothesized that epithelial-mesenchymal transition (EMT) contributes to the fibrotic process in trachomatous scarring. Infection of human conjunctival epithelial cells (HCjE) with Ct activated signaling pathways involved in EMT induction, which was correlated with decreased expression of E-cadherin, guardian of the epithelial phenotype. In addition, Ct infection was associated with increased expression of two mesenchymal cell markers: fibronectin and α-SMA. The DNA methylation statuses of selected regions of E-cadherin, fibronectin, and α-SMA genes revealed that Ct infection was accompanied with changes in DNA methylation of the E-cadherin promoter, while the expression of the two mesenchymal markers was not related with this epigenetic event. Our data suggest that Ct infection of conjunctival epithelial cells induces EMT-like changes that go along with modification of the methylation profile of the E-cadherin promoter and could, as one of the earliest events, contribute to processes triggering conjunctival scarring.",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Chlamydia trachomatis Infection Is Associated with E-Cadherin Promoter Methylation, Downregulation of E-Cadherin Expression, and Increased Expression of Fibronectin and α-SMA—Implications for Epithelial-Mesenchymal Transition",
number = "JUN",
volume = "7",
doi = "10.3389/fcimb.2017.00253",
pages = "253-253"
}

Rajić, J., Inic-Kanada, A., Stein, E., Dinić, S., Schuerer, N., Uskoković, A., Ghasemian, E., Mihailović, M., Vidaković, M., Grdović, N.,& Barisani-Asenbauer, T.. (2017). Chlamydia trachomatis Infection Is Associated with E-Cadherin Promoter Methylation, Downregulation of E-Cadherin Expression, and Increased Expression of Fibronectin and α-SMA—Implications for Epithelial-Mesenchymal Transition. in Frontiers in Cellular and Infection Microbiology, 7(JUN), 253-253.
https://doi.org/10.3389/fcimb.2017.00253

Rajić J, Inic-Kanada A, Stein E, Dinić S, Schuerer N, Uskoković A, Ghasemian E, Mihailović M, Vidaković M, Grdović N, Barisani-Asenbauer T. Chlamydia trachomatis Infection Is Associated with E-Cadherin Promoter Methylation, Downregulation of E-Cadherin Expression, and Increased Expression of Fibronectin and α-SMA—Implications for Epithelial-Mesenchymal Transition. in Frontiers in Cellular and Infection Microbiology. 2017;7(JUN):253-253.
doi:10.3389/fcimb.2017.00253 .

Rajić, Jovana, Inic-Kanada, Aleksandra, Stein, Elisabeth, Dinić, Svetlana, Schuerer, Nadine, Uskoković, Aleksandra, Ghasemian, Ehsan, Mihailović, Mirjana, Vidaković, Melita, Grdović, Nevena, Barisani-Asenbauer, Talin, "Chlamydia trachomatis Infection Is Associated with E-Cadherin Promoter Methylation, Downregulation of E-Cadherin Expression, and Increased Expression of Fibronectin and α-SMA—Implications for Epithelial-Mesenchymal Transition" in Frontiers in Cellular and Infection Microbiology, 7, no. JUN (2017):253-253,
https://doi.org/10.3389/fcimb.2017.00253 . .