TY  - JOUR
AU  - Miletić, Milica
AU  - Ivanov, Marija
AU  - Topalović, Aleksandra
AU  - Gavrilović, Milan
AU  - Gašić, Uroš
AU  - Janaćković, Peđa
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6465
AB  - Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts
IS  - 1
VL  - 47
DO  - 10.2298/BOTSERB2301093M
SP  - 93
EP  - 101
ER  - 

@article{
author = "Miletić, Milica and Ivanov, Marija and Topalović, Aleksandra and Gavrilović, Milan and Gašić, Uroš and Janaćković, Peđa",
year = "2023",
abstract = "Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts",
number = "1",
volume = "47",
doi = "10.2298/BOTSERB2301093M",
pages = "93-101"
}

Miletić, M., Ivanov, M., Topalović, A., Gavrilović, M., Gašić, U.,& Janaćković, P.. (2023). The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 47(1), 93-101.
https://doi.org/10.2298/BOTSERB2301093M

Miletić M, Ivanov M, Topalović A, Gavrilović M, Gašić U, Janaćković P. The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica. 2023;47(1):93-101.
doi:10.2298/BOTSERB2301093M .

Miletić, Milica, Ivanov, Marija, Topalović, Aleksandra, Gavrilović, Milan, Gašić, Uroš, Janaćković, Peđa, "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts" in Botanica Serbica, 47, no. 1 (2023):93-101,
https://doi.org/10.2298/BOTSERB2301093M . .

TY  - CONF
AU  - Miletić, Milica
AU  - Ivanov, Marija
AU  - Novaković, Jelica
AU  - Janaćković, Peđa
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6512
AB  - .Global health and modern medicine are facing a worldwide issue nowadays – antimicrobial resistance of microorganisms. A great interest in the pursuit of bioactive compounds from plants that can be antimicrobial drugs has been triggered due to multidrug resistance in pathogenic microorganisms and undesirable side effects of certain antibiotics (Alviano & Alviano, 2009). Ethnobotanical data suggest that many Centaurea species are traditionally used for treatment of various ailments in people and animals, as well as for nourishment (Khammar & Djeddi, 2012). Extracts obtained from Centaurea exhibit wide range of biological activity (Khammar & Djeddi, 2012) with a numerous studies concerning antimicrobial activity (Güven et al., 2005; Karamenderes et al., 2006; Özcan et al., 2019; Dimkić et al., 2020). Centaurea glaberrima Tausch (Asteraceae), Circum - Mediterranean Clade sensu Hilpold et al. (2014), is an endemic plant species that inhabits fields as well as a rocky cliffs and waste places in the West Balkans (Dostál, 1976). There is no information on biological activity of C. glaberrima therefore the aim of this study was to investigate antimicrobial potential of ethyl acetate extract of this endemic species.
PB  - Skopje: Macedonian Pharmaceutical Association
C3  - 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
T1  - Antimicrobial activity of ethyl acetate extract of an endemic Centaurea glaberrima Tausch (Asteraceae)
DO  - 10.33320/maced.pharm.bull.2022.68.04.036
SP  - 83
EP  - 84
ER  - 

@conference{
author = "Miletić, Milica and Ivanov, Marija and Novaković, Jelica and Janaćković, Peđa",
year = "2022",
abstract = ".Global health and modern medicine are facing a worldwide issue nowadays – antimicrobial resistance of microorganisms. A great interest in the pursuit of bioactive compounds from plants that can be antimicrobial drugs has been triggered due to multidrug resistance in pathogenic microorganisms and undesirable side effects of certain antibiotics (Alviano & Alviano, 2009). Ethnobotanical data suggest that many Centaurea species are traditionally used for treatment of various ailments in people and animals, as well as for nourishment (Khammar & Djeddi, 2012). Extracts obtained from Centaurea exhibit wide range of biological activity (Khammar & Djeddi, 2012) with a numerous studies concerning antimicrobial activity (Güven et al., 2005; Karamenderes et al., 2006; Özcan et al., 2019; Dimkić et al., 2020). Centaurea glaberrima Tausch (Asteraceae), Circum - Mediterranean Clade sensu Hilpold et al. (2014), is an endemic plant species that inhabits fields as well as a rocky cliffs and waste places in the West Balkans (Dostál, 1976). There is no information on biological activity of C. glaberrima therefore the aim of this study was to investigate antimicrobial potential of ethyl acetate extract of this endemic species.",
publisher = "Skopje: Macedonian Pharmaceutical Association",
journal = "11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia",
title = "Antimicrobial activity of ethyl acetate extract of an endemic Centaurea glaberrima Tausch (Asteraceae)",
doi = "10.33320/maced.pharm.bull.2022.68.04.036",
pages = "83-84"
}

Miletić, M., Ivanov, M., Novaković, J.,& Janaćković, P.. (2022). Antimicrobial activity of ethyl acetate extract of an endemic Centaurea glaberrima Tausch (Asteraceae). in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
Skopje: Macedonian Pharmaceutical Association., 83-84.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.036

Miletić M, Ivanov M, Novaković J, Janaćković P. Antimicrobial activity of ethyl acetate extract of an endemic Centaurea glaberrima Tausch (Asteraceae). in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia. 2022;:83-84.
doi:10.33320/maced.pharm.bull.2022.68.04.036 .

Miletić, Milica, Ivanov, Marija, Novaković, Jelica, Janaćković, Peđa, "Antimicrobial activity of ethyl acetate extract of an endemic Centaurea glaberrima Tausch (Asteraceae)" in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia (2022):83-84,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.036 . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Petrović, Marija
AU  - Gavrilović, Milan
AU  - Gašić, Uroš
AU  - Ristivojević, Petar
AU  - Stanković, Slaviša
AU  - Janaćković, Peđa
PY  - 2020
UR  - https://doi.org/10.1186/s13568-020-01120-5
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33044582
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7550514
UR  - https://radar.ibiss.bg.ac.rs/123456789/3919
AB  - Ethnobotanical and ethnopharmacological studies of many Centaurea species indicated their potential in folk medicine so far. However, investigations of different Centaurea calcitrapa L. extracts in terms of cytotoxicity and antimicrobial activity against phytopathogens are generally scarce. The phenolic profile and broad antimicrobial activity (especially towards bacterial phytopathogens) of methanol (MeOH), 70% ethanol (EtOH), ethyl-acetate (EtOAc), 50% acetone (Me2CO) and dichloromethane: methanol (DCM: MeOH, 1: 1) extracts of C. calcitrapa leaves and their potential toxicity on MRC-5 cell line were investigated for the first time. A total of 55 phenolic compounds were identified: 30 phenolic acids and their derivatives, 25 flavonoid glycosides and aglycones. This is also the first report of the presence of centaureidin, jaceidin, kaempferide, nepetin, flavonoid glycosides, phenolic acids and their esters in C. calcitrapa extracts. The best results were obtained with EtOAc extract with lowest MIC values expressed in µg/mL ranging from 13 to 25, while methicillin resistant Staphylococcus aureus was the most susceptible strain. The most susceptible phytopathogens were Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. campestris and Agrobacterium tumefaciens. The highest cytotoxicity was recorded for EtOAc and Me2CO extracts with the lowest relative and absolute IC50 values between 88 and 102 µg/mL, while EtOH extract was the least toxic with predicted relative IC50 value of 1578 µg/mL. Our results indicate that all tested extracts at concentration considered as non-toxic can be one of great importance in combat towards phytopathogenic and human pathogenic strains, as well as natural sources of antimicrobials.
PB  - Springer Berlin Heidelberg
T2  - AMB Express
T1  - New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity.
IS  - 1
VL  - 10
DO  - 10.1186/s13568-020-01120-5
SP  - 183
ER  - 

@article{
author = "Dimkić, Ivica and Petrović, Marija and Gavrilović, Milan and Gašić, Uroš and Ristivojević, Petar and Stanković, Slaviša and Janaćković, Peđa",
year = "2020",
abstract = "Ethnobotanical and ethnopharmacological studies of many Centaurea species indicated their potential in folk medicine so far. However, investigations of different Centaurea calcitrapa L. extracts in terms of cytotoxicity and antimicrobial activity against phytopathogens are generally scarce. The phenolic profile and broad antimicrobial activity (especially towards bacterial phytopathogens) of methanol (MeOH), 70% ethanol (EtOH), ethyl-acetate (EtOAc), 50% acetone (Me2CO) and dichloromethane: methanol (DCM: MeOH, 1: 1) extracts of C. calcitrapa leaves and their potential toxicity on MRC-5 cell line were investigated for the first time. A total of 55 phenolic compounds were identified: 30 phenolic acids and their derivatives, 25 flavonoid glycosides and aglycones. This is also the first report of the presence of centaureidin, jaceidin, kaempferide, nepetin, flavonoid glycosides, phenolic acids and their esters in C. calcitrapa extracts. The best results were obtained with EtOAc extract with lowest MIC values expressed in µg/mL ranging from 13 to 25, while methicillin resistant Staphylococcus aureus was the most susceptible strain. The most susceptible phytopathogens were Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. campestris and Agrobacterium tumefaciens. The highest cytotoxicity was recorded for EtOAc and Me2CO extracts with the lowest relative and absolute IC50 values between 88 and 102 µg/mL, while EtOH extract was the least toxic with predicted relative IC50 value of 1578 µg/mL. Our results indicate that all tested extracts at concentration considered as non-toxic can be one of great importance in combat towards phytopathogenic and human pathogenic strains, as well as natural sources of antimicrobials.",
publisher = "Springer Berlin Heidelberg",
journal = "AMB Express",
title = "New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity.",
number = "1",
volume = "10",
doi = "10.1186/s13568-020-01120-5",
pages = "183"
}

Dimkić, I., Petrović, M., Gavrilović, M., Gašić, U., Ristivojević, P., Stanković, S.,& Janaćković, P.. (2020). New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity.. in AMB Express
Springer Berlin Heidelberg., 10(1), 183.
https://doi.org/10.1186/s13568-020-01120-5

Dimkić I, Petrović M, Gavrilović M, Gašić U, Ristivojević P, Stanković S, Janaćković P. New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity.. in AMB Express. 2020;10(1):183.
doi:10.1186/s13568-020-01120-5 .

Dimkić, Ivica, Petrović, Marija, Gavrilović, Milan, Gašić, Uroš, Ristivojević, Petar, Stanković, Slaviša, Janaćković, Peđa, "New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity." in AMB Express, 10, no. 1 (2020):183,
https://doi.org/10.1186/s13568-020-01120-5 . .

TY  - JOUR
AU  - Janaćković, Peđa
AU  - Novaković, Jelica
AU  - Soković, Marina
AU  - Vujisić, Ljubodrag
AU  - Giweli, Abdulhmid A.
AU  - Dajić Stevanović, Zora
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2052
AB  - The essential oils obtained by hydrodistillation from the aerial parts
   of Artemisia judaica L., Artemisia herbaalba Asso. and Artemisia
   arborescens L. (cultivated) from Libya, were analyzed by GC and GC-MS.
   The antimicrobial properties were determined using the broth
   microdilution method against eight bacterial species: Bacillus cereus
   (clinical isolate), Micrococcus flavus (ATCC10240), Listeria
   monocytogenes (NCTC7973), Staphylococcus aureus (ATCC6538), Escherichia
   coli (ATCC35210), Pseudomonas aeruginosa (ATCC27853), Salmonella
   typhimurium (ATCC13311), Enterobacter cloacae (human isolates) and eight
   fungal species: Aspergillus niger (ATCC6275), A. ochraceus (ATCC12066),
   A. versicolor (ATCC11730), A. fumigatus (ATCC1022), Penicillium
   ochrochloron (ATCC9112), P. funiculosum (ATCC10509), Trichoderma viride
   (IAM5061) and Candida albicans (human isolate). The major constituents
   of A. arborescens oil were sesquiterpene hydrocarbons (47.4\%).
   Oxygenated monoterpenes were the dominant constituents in the A. judaica
   and A. herba-alba oils (54.2\% and 77.3\%, respectively). Camphor
   (24.7\%) and chamazulene (20.9\%) were the major components in the
   essential oil of A. arborescens, chrysanthenone (20.8\%),
   cis-chrysanthenyl acetate (17.6\%) and cis-thujone (13.6\%) dominated in
   the A. herba-alba oil, and the major constituents in the A. judaica oil
   were piperitone (30.21\%) and cis-chrysanthenol (9.1\%). The best
   antimicrobial activity was obtained for A. judaica oil and the lowest
   effect was noticed in A. arborescens oil. The effect of the tested oils
   was higher against Gram (+) than Gram (-) bacteria. All three oils
   showed the best antibacterial activity against Listeria monocytogenes
   and the lowest against Pseudomonas aeruginosa, Escherichia coli,
   Enterobacter cloacae, compared to streptomycin and ampicillin. All three
   oils showed better antifungal activities than ketoconazole, except A.
   arborescens oil against Aspergillus niger.
T2  - Archives of Biological Sciences
T1  - Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya
IS  - 2
VL  - 67
DO  - 10.2298/ABS141203010J
SP  - 455
EP  - 466
ER  - 

@article{
author = "Janaćković, Peđa and Novaković, Jelica and Soković, Marina and Vujisić, Ljubodrag and Giweli, Abdulhmid A. and Dajić Stevanović, Zora and Marin, Petar D.",
year = "2015",
abstract = "The essential oils obtained by hydrodistillation from the aerial parts
   of Artemisia judaica L., Artemisia herbaalba Asso. and Artemisia
   arborescens L. (cultivated) from Libya, were analyzed by GC and GC-MS.
   The antimicrobial properties were determined using the broth
   microdilution method against eight bacterial species: Bacillus cereus
   (clinical isolate), Micrococcus flavus (ATCC10240), Listeria
   monocytogenes (NCTC7973), Staphylococcus aureus (ATCC6538), Escherichia
   coli (ATCC35210), Pseudomonas aeruginosa (ATCC27853), Salmonella
   typhimurium (ATCC13311), Enterobacter cloacae (human isolates) and eight
   fungal species: Aspergillus niger (ATCC6275), A. ochraceus (ATCC12066),
   A. versicolor (ATCC11730), A. fumigatus (ATCC1022), Penicillium
   ochrochloron (ATCC9112), P. funiculosum (ATCC10509), Trichoderma viride
   (IAM5061) and Candida albicans (human isolate). The major constituents
   of A. arborescens oil were sesquiterpene hydrocarbons (47.4\%).
   Oxygenated monoterpenes were the dominant constituents in the A. judaica
   and A. herba-alba oils (54.2\% and 77.3\%, respectively). Camphor
   (24.7\%) and chamazulene (20.9\%) were the major components in the
   essential oil of A. arborescens, chrysanthenone (20.8\%),
   cis-chrysanthenyl acetate (17.6\%) and cis-thujone (13.6\%) dominated in
   the A. herba-alba oil, and the major constituents in the A. judaica oil
   were piperitone (30.21\%) and cis-chrysanthenol (9.1\%). The best
   antimicrobial activity was obtained for A. judaica oil and the lowest
   effect was noticed in A. arborescens oil. The effect of the tested oils
   was higher against Gram (+) than Gram (-) bacteria. All three oils
   showed the best antibacterial activity against Listeria monocytogenes
   and the lowest against Pseudomonas aeruginosa, Escherichia coli,
   Enterobacter cloacae, compared to streptomycin and ampicillin. All three
   oils showed better antifungal activities than ketoconazole, except A.
   arborescens oil against Aspergillus niger.",
journal = "Archives of Biological Sciences",
title = "Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya",
number = "2",
volume = "67",
doi = "10.2298/ABS141203010J",
pages = "455-466"
}

Janaćković, P., Novaković, J., Soković, M., Vujisić, L., Giweli, A. A., Dajić Stevanović, Z.,& Marin, P. D.. (2015). Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya. in Archives of Biological Sciences, 67(2), 455-466.
https://doi.org/10.2298/ABS141203010J

Janaćković P, Novaković J, Soković M, Vujisić L, Giweli AA, Dajić Stevanović Z, Marin PD. Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya. in Archives of Biological Sciences. 2015;67(2):455-466.
doi:10.2298/ABS141203010J .

Janaćković, Peđa, Novaković, Jelica, Soković, Marina, Vujisić, Ljubodrag, Giweli, Abdulhmid A., Dajić Stevanović, Zora, Marin, Petar D., "Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya" in Archives of Biological Sciences, 67, no. 2 (2015):455-466,
https://doi.org/10.2298/ABS141203010J . .