TY  - JOUR
AU  - Slavić, Marija
AU  - Đorđević, Aleksandar N
AU  - Radojičić, Ratko M.
AU  - Milovanović, Slobodan R
AU  - Oreščanin Dušić, Zorana
AU  - Rakocević, Zlatko Lj
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1012
AB  - Dimethyl sulfoxide (DMSO) is a widely used solvent and cryoprotectant that can cause impaired blood flow, reduction in intracranial pressure, tissue edema, inflammatory reactions, inhibition of vascular smooth muscle cell migration and proliferation, processes which can lead to atherosclerosis of the coronary, peripheral and cerebral circulation. Although the adverse effects are rare when DMSO is administered in clinically established concentrations, there is no safe antagonist for an overdose. In this work, we treated isolated spontaneous and calcium-induced contractile active rat uteri (Wistar, virgo intacta), with DMSO and fullerenol C-60(OH)(24) nanoparticle (FNP) in DMSO. FNP is a water-soluble derivative of fullerene C-60. Its size is a 1.1 nm in diameter and is a very promising candidate for a drug carrier in nanomedicine. FNP also displays free radical scavenging activity. DMSO decreased both spontaneous and calcium-induced contractions. In contrast, FNP only decreased spontaneous contraction. FNP decreased copper-zinc superoxide dismutase activity and prevented the DMSO-induced increase in glutathione reductase activity. Atomic force microscopy detected that FNP aggregated with calcium ions. Our results indicate that FNP has properties that make it a good candidate to be a modulator of DMSO activity which could minimize side effects of the latter.
T2  - Journal of Nanoparticle Research
T1  - Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction
IS  - 5
VL  - 15
SP  - 235
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1012
ER  - 

@article{
author = "Slavić, Marija and Đorđević, Aleksandar N and Radojičić, Ratko M. and Milovanović, Slobodan R and Oreščanin Dušić, Zorana and Rakocević, Zlatko Lj and Spasić, Mihajlo and Blagojević, Duško",
year = "2013",
abstract = "Dimethyl sulfoxide (DMSO) is a widely used solvent and cryoprotectant that can cause impaired blood flow, reduction in intracranial pressure, tissue edema, inflammatory reactions, inhibition of vascular smooth muscle cell migration and proliferation, processes which can lead to atherosclerosis of the coronary, peripheral and cerebral circulation. Although the adverse effects are rare when DMSO is administered in clinically established concentrations, there is no safe antagonist for an overdose. In this work, we treated isolated spontaneous and calcium-induced contractile active rat uteri (Wistar, virgo intacta), with DMSO and fullerenol C-60(OH)(24) nanoparticle (FNP) in DMSO. FNP is a water-soluble derivative of fullerene C-60. Its size is a 1.1 nm in diameter and is a very promising candidate for a drug carrier in nanomedicine. FNP also displays free radical scavenging activity. DMSO decreased both spontaneous and calcium-induced contractions. In contrast, FNP only decreased spontaneous contraction. FNP decreased copper-zinc superoxide dismutase activity and prevented the DMSO-induced increase in glutathione reductase activity. Atomic force microscopy detected that FNP aggregated with calcium ions. Our results indicate that FNP has properties that make it a good candidate to be a modulator of DMSO activity which could minimize side effects of the latter.",
journal = "Journal of Nanoparticle Research",
title = "Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction",
number = "5",
volume = "15",
pages = "235-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1012"
}

Slavić, M., Đorđević, A. N., Radojičić, R. M., Milovanović, S. R., Oreščanin Dušić, Z., Rakocević, Z. L., Spasić, M.,& Blagojević, D.. (2013). Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction. in Journal of Nanoparticle Research, 15(5), 235-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1012

Slavić M, Đorđević AN, Radojičić RM, Milovanović SR, Oreščanin Dušić Z, Rakocević ZL, Spasić M, Blagojević D. Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction. in Journal of Nanoparticle Research. 2013;15(5):235-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1012 .

Slavić, Marija, Đorđević, Aleksandar N, Radojičić, Ratko M., Milovanović, Slobodan R, Oreščanin Dušić, Zorana, Rakocević, Zlatko Lj, Spasić, Mihajlo, Blagojević, Duško, "Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction" in Journal of Nanoparticle Research, 15, no. 5 (2013):235-na,
https://hdl.handle.net/21.15107/rcub_ibiss_1012 .

TY  - JOUR
AU  - Appiah, Isabella
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojičić, Ratko M.
AU  - Oreščanin Dušić, Zorana
AU  - Milovanović, Slobodan R
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1222
AB  - The uteri, spontaneously active or Ca2+ (6 mM) induced, were allowed to equilibrate, and to inhibit voltage-gated potassium (KV) channels 1 mM 4-amino pyridine (4-AP) was applied for 15 min before adding H2O2. H2O2 was added cumulatively: 2 mu M, 20 mu M, 200 mu M, 400 mu M, and 3 mM. Average time for H2O2 concentrations (2, 20, 200, and 400) mu M to reach its full effect was 15 min. H2O2 3 mM had a prolonged effect and therefore was left to act for 30 min. Two-way ANOVA showed significant differences in time dependency between spontaneous and Ca2+-induced rat uteri after applying 3 mM H2O2 (type of contraction, P = 0.0280), but not 400 mu M H2O2 (P = 0.9271). Our results indicate that H2O2 oxidises channel intracellular thiol groups and activates the channel, inducing relaxation. Cell antioxidative defence system quickly activates glutathione peroxidase (GSHPx) defence mechanism but not catalase (CAT) defence mechanism. Intracellular redox mechanisms repair the oxidised sites and again establish deactivation of KV channels, recuperating contractility. In conclusion, our results demonstrate that KV channels can be altered in a time-dependent manner by reversible redox-dependent intracellular alterations.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide
IS  - null
VL  - null
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1222
ER  - 

@article{
author = "Appiah, Isabella and Nikolić-Kokić, Aleksandra and Radojičić, Ratko M. and Oreščanin Dušić, Zorana and Milovanović, Slobodan R and Spasić, Mihajlo and Blagojević, Duško",
year = "2012",
abstract = "The uteri, spontaneously active or Ca2+ (6 mM) induced, were allowed to equilibrate, and to inhibit voltage-gated potassium (KV) channels 1 mM 4-amino pyridine (4-AP) was applied for 15 min before adding H2O2. H2O2 was added cumulatively: 2 mu M, 20 mu M, 200 mu M, 400 mu M, and 3 mM. Average time for H2O2 concentrations (2, 20, 200, and 400) mu M to reach its full effect was 15 min. H2O2 3 mM had a prolonged effect and therefore was left to act for 30 min. Two-way ANOVA showed significant differences in time dependency between spontaneous and Ca2+-induced rat uteri after applying 3 mM H2O2 (type of contraction, P = 0.0280), but not 400 mu M H2O2 (P = 0.9271). Our results indicate that H2O2 oxidises channel intracellular thiol groups and activates the channel, inducing relaxation. Cell antioxidative defence system quickly activates glutathione peroxidase (GSHPx) defence mechanism but not catalase (CAT) defence mechanism. Intracellular redox mechanisms repair the oxidised sites and again establish deactivation of KV channels, recuperating contractility. In conclusion, our results demonstrate that KV channels can be altered in a time-dependent manner by reversible redox-dependent intracellular alterations.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide",
number = "null",
volume = "null",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1222"
}

Appiah, I., Nikolić-Kokić, A., Radojičić, R. M., Oreščanin Dušić, Z., Milovanović, S. R., Spasić, M.,& Blagojević, D.. (2012). Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide. in Oxidative Medicine and Cellular Longevity, null(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1222

Appiah I, Nikolić-Kokić A, Radojičić RM, Oreščanin Dušić Z, Milovanović SR, Spasić M, Blagojević D. Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide. in Oxidative Medicine and Cellular Longevity. 2012;null(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1222 .

Appiah, Isabella, Nikolić-Kokić, Aleksandra, Radojičić, Ratko M., Oreščanin Dušić, Zorana, Milovanović, Slobodan R, Spasić, Mihajlo, Blagojević, Duško, "Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide" in Oxidative Medicine and Cellular Longevity, null, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1222 .

TY  - JOUR
AU  - Appiah, Isabella
AU  - Milovanović, Slobodan R
AU  - Radojičić, Ratko M.
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Trbojević, Stevan
AU  - Skrbić, Ranko
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1421
AB  - Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.
T2  - British Journal of Pharmacology
T1  - Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus
IS  - 8
VL  - 158
EP  - 1941
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1421
ER  - 

@article{
author = "Appiah, Isabella and Milovanović, Slobodan R and Radojičić, Ratko M. and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Slavić, Marija and Trbojević, Stevan and Skrbić, Ranko and Spasić, Mihajlo and Blagojević, Duško",
year = "2009",
abstract = "Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.",
journal = "British Journal of Pharmacology",
title = "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus",
number = "8",
volume = "158",
pages = "1941",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1421"
}

Appiah, I., Milovanović, S. R., Radojičić, R. M., Nikolić-Kokić, A., Oreščanin Dušić, Z., Slavić, M., Trbojević, S., Skrbić, R., Spasić, M.,& Blagojević, D.. (2009). Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology, 158(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1421

Appiah I, Milovanović SR, Radojičić RM, Nikolić-Kokić A, Oreščanin Dušić Z, Slavić M, Trbojević S, Skrbić R, Spasić M, Blagojević D. Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology. 2009;158(8):null-1941.
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .

Appiah, Isabella, Milovanović, Slobodan R, Radojičić, Ratko M., Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Slavić, Marija, Trbojević, Stevan, Skrbić, Ranko, Spasić, Mihajlo, Blagojević, Duško, "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus" in British Journal of Pharmacology, 158, no. 8 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .

TY  - JOUR
AU  - Milovanović, Slobodan R
AU  - Oreščanin Dušić, Zorana
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojičić, Ratko M.
AU  - Spasojević, Ivan B
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1457
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC(2) complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC(2) complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
IS  - 2
VL  - 14
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1457
ER  - 

@article{
author = "Milovanović, Slobodan R and Oreščanin Dušić, Zorana and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojičić, Ratko M. and Spasojević, Ivan B and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC(2) complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC(2) complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
number = "2",
volume = "14",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1457"
}

Milovanović, S. R., Oreščanin Dušić, Z., Blagojević, D., Nikolić-Kokić, A., Radojičić, R. M., Spasojević, I. B.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report, 14(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1457

Milovanović SR, Oreščanin Dušić Z, Blagojević D, Nikolić-Kokić A, Radojičić RM, Spasojević IB, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):null-54.
https://hdl.handle.net/21.15107/rcub_ibiss_1457 .

Milovanović, Slobodan R, Oreščanin Dušić, Zorana, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojičić, Ratko M., Spasojević, Ivan B, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1457 .

TY  - JOUR
AU  - Blagojević, Duško
AU  - Oreščanin Dušić, Zorana
AU  - Milovanović, Slobodan R
AU  - Radojičić, Ratko M.
AU  - Nikolić-Kokić, Aleksandra
AU  - Appiah, Isabella
AU  - Slavić, Marija
AU  - Cutura, Neđo D
AU  - Trbojević, Stevan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1475
AB  - Protamine sulphate (PS) effect on spontaneous and calcium-induced rhythmic contractions of isolated virgin rat uteri was studied. PS caused dose-dependent relaxation of both types of contractions (two-way ANOVA, significant dose effects). Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10(-5) mol/l), methylene blue (MB; 0.9 x 10(-6) mol/l) or propranolol (1.7 x 10(-5) mol/l) enhanced PS-mediated uterine muscle relaxation of spontaneous contractions. Dose-dependent relaxation of spontaneous active isolated rat uterus with PS was lower in uteri pretreated with single dose of tetraethylammonium (TEA; 6 x 10(-3) mol/l), glibenclamide (2 x 10(-6) mol/l) and 4-aminopyridine (4-AP; 10(-3) mol/l). Calcium-induced activity of the isolated rat uterus pretreated with the same concentration of L-NAME, MB, or propranolol modified the kinetic of PS-induced relaxation without changes in EC(50) values. Pre-treatment with glibenclamide, TEA and 4-AP significantly reduce PS relaxing effect of calcium-induced activity and according to EC(50) values the order of magnitude was glibenclamide > TEA > 4-AP. PS is mixture of polyamines and may activate different signal-transduction pathways. Our results cleary demonstrate that in uterine smooth muscle PS act dominantly through potassium chanels and marginaly through beta-adrenergic receptos or nitric oxide-dependent pathways.
T2  - General Physiology and Biophysics
T1  - Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated
IS  - null
VL  - 28
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1475
ER  - 

@article{
author = "Blagojević, Duško and Oreščanin Dušić, Zorana and Milovanović, Slobodan R and Radojičić, Ratko M. and Nikolić-Kokić, Aleksandra and Appiah, Isabella and Slavić, Marija and Cutura, Neđo D and Trbojević, Stevan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate (PS) effect on spontaneous and calcium-induced rhythmic contractions of isolated virgin rat uteri was studied. PS caused dose-dependent relaxation of both types of contractions (two-way ANOVA, significant dose effects). Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10(-5) mol/l), methylene blue (MB; 0.9 x 10(-6) mol/l) or propranolol (1.7 x 10(-5) mol/l) enhanced PS-mediated uterine muscle relaxation of spontaneous contractions. Dose-dependent relaxation of spontaneous active isolated rat uterus with PS was lower in uteri pretreated with single dose of tetraethylammonium (TEA; 6 x 10(-3) mol/l), glibenclamide (2 x 10(-6) mol/l) and 4-aminopyridine (4-AP; 10(-3) mol/l). Calcium-induced activity of the isolated rat uterus pretreated with the same concentration of L-NAME, MB, or propranolol modified the kinetic of PS-induced relaxation without changes in EC(50) values. Pre-treatment with glibenclamide, TEA and 4-AP significantly reduce PS relaxing effect of calcium-induced activity and according to EC(50) values the order of magnitude was glibenclamide > TEA > 4-AP. PS is mixture of polyamines and may activate different signal-transduction pathways. Our results cleary demonstrate that in uterine smooth muscle PS act dominantly through potassium chanels and marginaly through beta-adrenergic receptos or nitric oxide-dependent pathways.",
journal = "General Physiology and Biophysics",
title = "Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated",
number = "null",
volume = "28",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1475"
}

Blagojević, D., Oreščanin Dušić, Z., Milovanović, S. R., Radojičić, R. M., Nikolić-Kokić, A., Appiah, I., Slavić, M., Cutura, N. D., Trbojević, S.,& Spasić, M.. (2009). Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1475

Blagojević D, Oreščanin Dušić Z, Milovanović SR, Radojičić RM, Nikolić-Kokić A, Appiah I, Slavić M, Cutura ND, Trbojević S, Spasić M. Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated. in General Physiology and Biophysics. 2009;28(null):null-148.
https://hdl.handle.net/21.15107/rcub_ibiss_1475 .

Blagojević, Duško, Oreščanin Dušić, Zorana, Milovanović, Slobodan R, Radojičić, Ratko M., Nikolić-Kokić, Aleksandra, Appiah, Isabella, Slavić, Marija, Cutura, Neđo D, Trbojević, Stevan, Spasić, Mihajlo, "Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1475 .

TY  - JOUR
AU  - Tepavcević, Snezana N
AU  - Isenović, Esma R
AU  - Varagić, Vladislav M
AU  - Milovanović, Slobodan R
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1547
AB  - Nitric oxide (NO) has been shown to mediate nonadrenergic-noncholinergic relaxation in gastrointestinal (GI) smooth muscle cells. As GI smooth muscles relaxations are partly dependent on NO, we decided to investigate the effect of sodium nitroprusside (SNP) on the longitudinal muscle contraction of the isolated guinea pig ileum. Increasing concentrations of SNP (10(-10)M, 10(-9)M, 10(-8)M, 10(-7)M, 10(-6)M and 10(-5)M) reduced ileum contractions stimulated by electrical stimulation (ES) (8-76%; p < 0.05) and by acetylcholine (Ach) (23-62%; p < 0.05) significantly and in a concentration-dependent manner. Furthermore, treatment with an inhibitor of the soluble guanylate cyclase, methylene blue (10 mM), antagonized significantly the relaxing effect of SNP (0-39%; p < 0.05, p < 0.01, p < 0.001 for ES- and 4-27%; p < 0.05 for Ach-induced contractions). The results show that treatment with 1 mu M manganese-containing superoxide dismutase (MnSOD) and 10 mu M L-arginine (L-arg) caused a significant decrease in SNP induced relaxations (6-55%; p < 0.05, p < 0.001 and 2-46%; p < 0.05, p < 0.01 for ES- and 15-28%; p < 0.05, p < 0.01, p < 0.001 and 12-32%; p < 0.05, p < 0.01 for Ach-induced contractions, respectively). In conclusion, our data suggest that SNP, which releases NO, is able to depress longitudinal muscle contraction of the isolated guinea pig ileum, suggesting that exogenous application of NO inhibits intestinal contractions of smooth muscle cells and that cGMP mediates the response to NO. In addition, MnSOD and L-arg decreased the relaxing effect of SNP on the isolated ileum of the guinea pig.
T2  - Pharmazie
T1  - Sodium nitroprusside regulates the relaxation of the longitudinal muscle in the gut
IS  - 2
VL  - 63
EP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1547
ER  - 

@article{
author = "Tepavcević, Snezana N and Isenović, Esma R and Varagić, Vladislav M and Milovanović, Slobodan R",
year = "2008",
abstract = "Nitric oxide (NO) has been shown to mediate nonadrenergic-noncholinergic relaxation in gastrointestinal (GI) smooth muscle cells. As GI smooth muscles relaxations are partly dependent on NO, we decided to investigate the effect of sodium nitroprusside (SNP) on the longitudinal muscle contraction of the isolated guinea pig ileum. Increasing concentrations of SNP (10(-10)M, 10(-9)M, 10(-8)M, 10(-7)M, 10(-6)M and 10(-5)M) reduced ileum contractions stimulated by electrical stimulation (ES) (8-76%; p < 0.05) and by acetylcholine (Ach) (23-62%; p < 0.05) significantly and in a concentration-dependent manner. Furthermore, treatment with an inhibitor of the soluble guanylate cyclase, methylene blue (10 mM), antagonized significantly the relaxing effect of SNP (0-39%; p < 0.05, p < 0.01, p < 0.001 for ES- and 4-27%; p < 0.05 for Ach-induced contractions). The results show that treatment with 1 mu M manganese-containing superoxide dismutase (MnSOD) and 10 mu M L-arginine (L-arg) caused a significant decrease in SNP induced relaxations (6-55%; p < 0.05, p < 0.001 and 2-46%; p < 0.05, p < 0.01 for ES- and 15-28%; p < 0.05, p < 0.01, p < 0.001 and 12-32%; p < 0.05, p < 0.01 for Ach-induced contractions, respectively). In conclusion, our data suggest that SNP, which releases NO, is able to depress longitudinal muscle contraction of the isolated guinea pig ileum, suggesting that exogenous application of NO inhibits intestinal contractions of smooth muscle cells and that cGMP mediates the response to NO. In addition, MnSOD and L-arg decreased the relaxing effect of SNP on the isolated ileum of the guinea pig.",
journal = "Pharmazie",
title = "Sodium nitroprusside regulates the relaxation of the longitudinal muscle in the gut",
number = "2",
volume = "63",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1547"
}

Tepavcević, S. N., Isenović, E. R., Varagić, V. M.,& Milovanović, S. R.. (2008). Sodium nitroprusside regulates the relaxation of the longitudinal muscle in the gut. in Pharmazie, 63(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1547

Tepavcević SN, Isenović ER, Varagić VM, Milovanović SR. Sodium nitroprusside regulates the relaxation of the longitudinal muscle in the gut. in Pharmazie. 2008;63(2):null-155.
https://hdl.handle.net/21.15107/rcub_ibiss_1547 .

Tepavcević, Snezana N, Isenović, Esma R, Varagić, Vladislav M, Milovanović, Slobodan R, "Sodium nitroprusside regulates the relaxation of the longitudinal muscle in the gut" in Pharmazie, 63, no. 2 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1547 .

TY  - JOUR
AU  - Oreščanin Dušić, Zorana
AU  - Milovanović, Slobodan R
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1605
AB  - The conversion of nitric oxide (NO*) into its congeners nitrosonium (NO+) and nitroxyl (HNO/NO-) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert NO* into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO--mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not solely endothelium-derived but involves changes in vascular smooth muscles.
T2  - Pharmacological Reports
T1  - Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners
IS  - 3
VL  - 59
EP  - 322
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1605
ER  - 

@article{
author = "Oreščanin Dušić, Zorana and Milovanović, Slobodan R and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2007",
abstract = "The conversion of nitric oxide (NO*) into its congeners nitrosonium (NO+) and nitroxyl (HNO/NO-) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert NO* into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO--mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not solely endothelium-derived but involves changes in vascular smooth muscles.",
journal = "Pharmacological Reports",
title = "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners",
number = "3",
volume = "59",
pages = "322",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1605"
}

Oreščanin Dušić, Z., Milovanović, S. R., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2007). Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Reports, 59(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1605

Oreščanin Dušić Z, Milovanović SR, Spasić SD, Jones DR, Spasić M. Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Reports. 2007;59(3):null-322.
https://hdl.handle.net/21.15107/rcub_ibiss_1605 .

Oreščanin Dušić, Zorana, Milovanović, Slobodan R, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners" in Pharmacological Reports, 59, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1605 .