TY  - JOUR
AU  - Appiah, Isabella
AU  - Milovanović, Slobodan R
AU  - Radojičić, Ratko M.
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Trbojević, Stevan
AU  - Skrbić, Ranko
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1421
AB  - Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.
T2  - British Journal of Pharmacology
T1  - Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus
IS  - 8
VL  - 158
EP  - 1941
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1421
ER  - 

@article{
author = "Appiah, Isabella and Milovanović, Slobodan R and Radojičić, Ratko M. and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Slavić, Marija and Trbojević, Stevan and Skrbić, Ranko and Spasić, Mihajlo and Blagojević, Duško",
year = "2009",
abstract = "Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.",
journal = "British Journal of Pharmacology",
title = "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus",
number = "8",
volume = "158",
pages = "1941",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1421"
}

Appiah, I., Milovanović, S. R., Radojičić, R. M., Nikolić-Kokić, A., Oreščanin Dušić, Z., Slavić, M., Trbojević, S., Skrbić, R., Spasić, M.,& Blagojević, D.. (2009). Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology, 158(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1421

Appiah I, Milovanović SR, Radojičić RM, Nikolić-Kokić A, Oreščanin Dušić Z, Slavić M, Trbojević S, Skrbić R, Spasić M, Blagojević D. Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology. 2009;158(8):null-1941.
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .

Appiah, Isabella, Milovanović, Slobodan R, Radojičić, Ratko M., Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Slavić, Marija, Trbojević, Stevan, Skrbić, Ranko, Spasić, Mihajlo, Blagojević, Duško, "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus" in British Journal of Pharmacology, 158, no. 8 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .

TY  - JOUR
AU  - Blagojević, Duško
AU  - Oreščanin Dušić, Zorana
AU  - Milovanović, Slobodan R
AU  - Radojičić, Ratko M.
AU  - Nikolić-Kokić, Aleksandra
AU  - Appiah, Isabella
AU  - Slavić, Marija
AU  - Cutura, Neđo D
AU  - Trbojević, Stevan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1475
AB  - Protamine sulphate (PS) effect on spontaneous and calcium-induced rhythmic contractions of isolated virgin rat uteri was studied. PS caused dose-dependent relaxation of both types of contractions (two-way ANOVA, significant dose effects). Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10(-5) mol/l), methylene blue (MB; 0.9 x 10(-6) mol/l) or propranolol (1.7 x 10(-5) mol/l) enhanced PS-mediated uterine muscle relaxation of spontaneous contractions. Dose-dependent relaxation of spontaneous active isolated rat uterus with PS was lower in uteri pretreated with single dose of tetraethylammonium (TEA; 6 x 10(-3) mol/l), glibenclamide (2 x 10(-6) mol/l) and 4-aminopyridine (4-AP; 10(-3) mol/l). Calcium-induced activity of the isolated rat uterus pretreated with the same concentration of L-NAME, MB, or propranolol modified the kinetic of PS-induced relaxation without changes in EC(50) values. Pre-treatment with glibenclamide, TEA and 4-AP significantly reduce PS relaxing effect of calcium-induced activity and according to EC(50) values the order of magnitude was glibenclamide > TEA > 4-AP. PS is mixture of polyamines and may activate different signal-transduction pathways. Our results cleary demonstrate that in uterine smooth muscle PS act dominantly through potassium chanels and marginaly through beta-adrenergic receptos or nitric oxide-dependent pathways.
T2  - General Physiology and Biophysics
T1  - Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated
IS  - null
VL  - 28
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1475
ER  - 

@article{
author = "Blagojević, Duško and Oreščanin Dušić, Zorana and Milovanović, Slobodan R and Radojičić, Ratko M. and Nikolić-Kokić, Aleksandra and Appiah, Isabella and Slavić, Marija and Cutura, Neđo D and Trbojević, Stevan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate (PS) effect on spontaneous and calcium-induced rhythmic contractions of isolated virgin rat uteri was studied. PS caused dose-dependent relaxation of both types of contractions (two-way ANOVA, significant dose effects). Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10(-5) mol/l), methylene blue (MB; 0.9 x 10(-6) mol/l) or propranolol (1.7 x 10(-5) mol/l) enhanced PS-mediated uterine muscle relaxation of spontaneous contractions. Dose-dependent relaxation of spontaneous active isolated rat uterus with PS was lower in uteri pretreated with single dose of tetraethylammonium (TEA; 6 x 10(-3) mol/l), glibenclamide (2 x 10(-6) mol/l) and 4-aminopyridine (4-AP; 10(-3) mol/l). Calcium-induced activity of the isolated rat uterus pretreated with the same concentration of L-NAME, MB, or propranolol modified the kinetic of PS-induced relaxation without changes in EC(50) values. Pre-treatment with glibenclamide, TEA and 4-AP significantly reduce PS relaxing effect of calcium-induced activity and according to EC(50) values the order of magnitude was glibenclamide > TEA > 4-AP. PS is mixture of polyamines and may activate different signal-transduction pathways. Our results cleary demonstrate that in uterine smooth muscle PS act dominantly through potassium chanels and marginaly through beta-adrenergic receptos or nitric oxide-dependent pathways.",
journal = "General Physiology and Biophysics",
title = "Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated",
number = "null",
volume = "28",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1475"
}

Blagojević, D., Oreščanin Dušić, Z., Milovanović, S. R., Radojičić, R. M., Nikolić-Kokić, A., Appiah, I., Slavić, M., Cutura, N. D., Trbojević, S.,& Spasić, M.. (2009). Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1475

Blagojević D, Oreščanin Dušić Z, Milovanović SR, Radojičić RM, Nikolić-Kokić A, Appiah I, Slavić M, Cutura ND, Trbojević S, Spasić M. Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated. in General Physiology and Biophysics. 2009;28(null):null-148.
https://hdl.handle.net/21.15107/rcub_ibiss_1475 .

Blagojević, Duško, Oreščanin Dušić, Zorana, Milovanović, Slobodan R, Radojičić, Ratko M., Nikolić-Kokić, Aleksandra, Appiah, Isabella, Slavić, Marija, Cutura, Neđo D, Trbojević, Stevan, Spasić, Mihajlo, "Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1475 .