TY  - JOUR
AU  - Sarac, Zorica
AU  - Matejic, Jelena S.
AU  - Stojanovic-Radic, Zorica Z.
AU  - Veselinovic, Jovana B.
AU  - Džamić, Ana M.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2127
AB  - In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Computers in Biology and Medicine
T1  - Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity
VL  - 54
DO  - 10.1016/j.compbiomed.2014.08.022
SP  - 72
EP  - 78
ER  - 

@article{
author = "Sarac, Zorica and Matejic, Jelena S. and Stojanovic-Radic, Zorica Z. and Veselinovic, Jovana B. and Džamić, Ana M. and Bojović, Srđan and Marin, Petar D.",
year = "2014",
abstract = "In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Computers in Biology and Medicine",
title = "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity",
volume = "54",
doi = "10.1016/j.compbiomed.2014.08.022",
pages = "72-78"
}

Sarac, Z., Matejic, J. S., Stojanovic-Radic, Z. Z., Veselinovic, J. B., Džamić, A. M., Bojović, S.,& Marin, P. D.. (2014). Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine, 54, 72-78.
https://doi.org/10.1016/j.compbiomed.2014.08.022

Sarac Z, Matejic JS, Stojanovic-Radic ZZ, Veselinovic JB, Džamić AM, Bojović S, Marin PD. Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine. 2014;54:72-78.
doi:10.1016/j.compbiomed.2014.08.022 .

Sarac, Zorica, Matejic, Jelena S., Stojanovic-Radic, Zorica Z., Veselinovic, Jovana B., Džamić, Ana M., Bojović, Srđan, Marin, Petar D., "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity" in Computers in Biology and Medicine, 54 (2014):72-78,
https://doi.org/10.1016/j.compbiomed.2014.08.022 . .