TY  - CONF
AU  - Jovanović, Irena
AU  - Nedeljković, Milica
AU  - Medić-Milijić, Nataša
AU  - Spurnić, Igor
AU  - Milovanović, Zorka
AU  - Tomić, Tijana
AU  - Tanić, Nasta
AU  - Tanić, Nikola
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6324
AB  - Background: Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and is associated with high recurrence rates, high incidence of distant metastases and poor overall survival. The aim of this study was to investigate the role of cludin 3, claudin 4 and claudin 7 in TNBC promotion and progression. Claudins are tight junction (TJ) integral membrane proteins that are key regulators of the paracellular pathway. Materials and methods: This is a retrospective analysis of 125 patients with triple-negative breast cancer operated at the Institute of Oncology and Radiology of Serbia in the period from 2009 to 2014. The expression of claudin 3, 4 and 7 was observed using the immunohistochemical staining method. The Allred scoring system was used with cut-off values: ≤4 and >4 (low/high expression). Results: Our results showed that the expression of claudins 3 and 4 correlate with higher nuclear gradus and low desease free interval (DFI). More over, the expression of claudin 3 and claudin 4 correlates (Spearman test p˂0.0001). In addition, high expression of claudin 7 is signifi cantly related to low DFI of patients (p˂0.005) and distant metastases. Conclusions: We concluded that claudin 3, claudin 4 and claudin 7 have significant impact on TNBC progression. Namely, elevated expression of these proteins significantly correlates with low DFI and distant metastases. In other words, elevated expression of claudins is a bad news for TNBC patients. Therefore, the expression of claudins could be a good prognostic marker for TNBC patients and potential target for future therapy protocols.
PB  - Belgrade: Serbian Association for Cancer Research
C3  - Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
T1  - Role of claudins 3,4 and 7 in triple negative breast cancer progression
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6324
ER  - 

@conference{
author = "Jovanović, Irena and Nedeljković, Milica and Medić-Milijić, Nataša and Spurnić, Igor and Milovanović, Zorka and Tomić, Tijana and Tanić, Nasta and Tanić, Nikola",
year = "2023",
abstract = "Background: Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and is associated with high recurrence rates, high incidence of distant metastases and poor overall survival. The aim of this study was to investigate the role of cludin 3, claudin 4 and claudin 7 in TNBC promotion and progression. Claudins are tight junction (TJ) integral membrane proteins that are key regulators of the paracellular pathway. Materials and methods: This is a retrospective analysis of 125 patients with triple-negative breast cancer operated at the Institute of Oncology and Radiology of Serbia in the period from 2009 to 2014. The expression of claudin 3, 4 and 7 was observed using the immunohistochemical staining method. The Allred scoring system was used with cut-off values: ≤4 and >4 (low/high expression). Results: Our results showed that the expression of claudins 3 and 4 correlate with higher nuclear gradus and low desease free interval (DFI). More over, the expression of claudin 3 and claudin 4 correlates (Spearman test p˂0.0001). In addition, high expression of claudin 7 is signifi cantly related to low DFI of patients (p˂0.005) and distant metastases. Conclusions: We concluded that claudin 3, claudin 4 and claudin 7 have significant impact on TNBC progression. Namely, elevated expression of these proteins significantly correlates with low DFI and distant metastases. In other words, elevated expression of claudins is a bad news for TNBC patients. Therefore, the expression of claudins could be a good prognostic marker for TNBC patients and potential target for future therapy protocols.",
publisher = "Belgrade: Serbian Association for Cancer Research",
journal = "Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia",
title = "Role of claudins 3,4 and 7 in triple negative breast cancer progression",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6324"
}

Jovanović, I., Nedeljković, M., Medić-Milijić, N., Spurnić, I., Milovanović, Z., Tomić, T., Tanić, N.,& Tanić, N.. (2023). Role of claudins 3,4 and 7 in triple negative breast cancer progression. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
Belgrade: Serbian Association for Cancer Research., 63.
https://hdl.handle.net/21.15107/rcub_ibiss_6324

Jovanović I, Nedeljković M, Medić-Milijić N, Spurnić I, Milovanović Z, Tomić T, Tanić N, Tanić N. Role of claudins 3,4 and 7 in triple negative breast cancer progression. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia. 2023;:63.
https://hdl.handle.net/21.15107/rcub_ibiss_6324 .

Jovanović, Irena, Nedeljković, Milica, Medić-Milijić, Nataša, Spurnić, Igor, Milovanović, Zorka, Tomić, Tijana, Tanić, Nasta, Tanić, Nikola, "Role of claudins 3,4 and 7 in triple negative breast cancer progression" in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia (2023):63,
https://hdl.handle.net/21.15107/rcub_ibiss_6324 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Dragičević, Milan B.
AU  - Tanić, Nikola T
AU  - Todorović, Slađana
AU  - Mišić, Danijela
AU  - Živković, Suzana
AU  - Tissier, Alain
AU  - Simonović, Ana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1045
AB  - Ribosomal 18S RNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern analysis, and real-time experiments. However, there are two disadvantages and two points of error introduction in using 18S rRNA as a reference gene. First, 18S has no poly(A) tail, so it is commonly reverse transcribed with specific primers or random hexamers, independently from poly(dT)-primed transcripts. Secondly, due to its abundance, the 18S cDNA must be extensively diluted to be comparable to the tested genes. In this study, 18S rRNA from five taxonomically diverse plant species, including Physcomitrella patens, Adiantum capillus-veneris, Centaurium erythraea, Arabidopsis thaliana, and Zea mays, was successfully reverse transcribed (RT) using poly(dT)(18). As all other homopolymers, including poly(dA)(18), poly(dC)(18), and poly(dG)(18), could serve as RT primers, it was concluded that homopolymers anneal by mispriming at the sites of complementary homopolymeric runs or segments rich in complementary base. Poly(dC)(18) was the most efficient as RT primer, and the only one which interfered with subsequent PCR, giving species-specific pattern of products. Poly(dT)-primed RT reactions were less efficient in comparison to specific primer or random hexamer-primed reactions. Homopolymeric priming of 18S in RT reactions is general in terms of RNA origin and the method of RNA isolation and is possibly applicable to other tailless housekeeping genes.
T2  - Plant Molecular Biology Reporter
T1  - Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers
IS  - 1
VL  - 31
SP  - 1
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1045
ER  - 

@article{
author = "Bogdanović, Milica and Dragičević, Milan B. and Tanić, Nikola T and Todorović, Slađana and Mišić, Danijela and Živković, Suzana and Tissier, Alain and Simonović, Ana",
year = "2013",
abstract = "Ribosomal 18S RNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern analysis, and real-time experiments. However, there are two disadvantages and two points of error introduction in using 18S rRNA as a reference gene. First, 18S has no poly(A) tail, so it is commonly reverse transcribed with specific primers or random hexamers, independently from poly(dT)-primed transcripts. Secondly, due to its abundance, the 18S cDNA must be extensively diluted to be comparable to the tested genes. In this study, 18S rRNA from five taxonomically diverse plant species, including Physcomitrella patens, Adiantum capillus-veneris, Centaurium erythraea, Arabidopsis thaliana, and Zea mays, was successfully reverse transcribed (RT) using poly(dT)(18). As all other homopolymers, including poly(dA)(18), poly(dC)(18), and poly(dG)(18), could serve as RT primers, it was concluded that homopolymers anneal by mispriming at the sites of complementary homopolymeric runs or segments rich in complementary base. Poly(dC)(18) was the most efficient as RT primer, and the only one which interfered with subsequent PCR, giving species-specific pattern of products. Poly(dT)-primed RT reactions were less efficient in comparison to specific primer or random hexamer-primed reactions. Homopolymeric priming of 18S in RT reactions is general in terms of RNA origin and the method of RNA isolation and is possibly applicable to other tailless housekeeping genes.",
journal = "Plant Molecular Biology Reporter",
title = "Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers",
number = "1",
volume = "31",
pages = "1-63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1045"
}

Bogdanović, M., Dragičević, M. B., Tanić, N. T., Todorović, S., Mišić, D., Živković, S., Tissier, A.,& Simonović, A.. (2013). Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers. in Plant Molecular Biology Reporter, 31(1), 1-63.
https://hdl.handle.net/21.15107/rcub_ibiss_1045

Bogdanović M, Dragičević MB, Tanić NT, Todorović S, Mišić D, Živković S, Tissier A, Simonović A. Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers. in Plant Molecular Biology Reporter. 2013;31(1):1-63.
https://hdl.handle.net/21.15107/rcub_ibiss_1045 .

Bogdanović, Milica, Dragičević, Milan B., Tanić, Nikola T, Todorović, Slađana, Mišić, Danijela, Živković, Suzana, Tissier, Alain, Simonović, Ana, "Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers" in Plant Molecular Biology Reporter, 31, no. 1 (2013):1-63,
https://hdl.handle.net/21.15107/rcub_ibiss_1045 .

TY  - JOUR
AU  - Fischedick, Justin T
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Banković, Jasna Z.
AU  - de Vos, Ric CH
AU  - Perić, Marija
AU  - Todorović, Slađana
AU  - Tanić, Nikola T
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1016
AB  - Five new sesquiterpene lactones (1-5) were isolated from Inula britannica collected in the wild from Serbia along with five known compounds (6-10). Sesquiterpene lactones were isolated using centrifugal partition chromatography followed by combination of flash chromatography and semi-preparative HPLC. Isolated compounds were screened for cytotoxic activity on four different human cancer cell lines and their multi-drug resistant counterparts, as well as on normal human keratinocytes. Sesquiterpene lactones showed similar cytotoxic activity toward drug sensitive and drug resistant cancer cell lines. (C) 2013 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.
T2  - Phytochemistry Letters
T1  - Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines
IS  - 2
VL  - 6
SP  - 1
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1016
ER  - 

@article{
author = "Fischedick, Justin T and Pešić, Milica and Podolski-Renić, Ana and Banković, Jasna Z. and de Vos, Ric CH and Perić, Marija and Todorović, Slađana and Tanić, Nikola T",
year = "2013",
abstract = "Five new sesquiterpene lactones (1-5) were isolated from Inula britannica collected in the wild from Serbia along with five known compounds (6-10). Sesquiterpene lactones were isolated using centrifugal partition chromatography followed by combination of flash chromatography and semi-preparative HPLC. Isolated compounds were screened for cytotoxic activity on four different human cancer cell lines and their multi-drug resistant counterparts, as well as on normal human keratinocytes. Sesquiterpene lactones showed similar cytotoxic activity toward drug sensitive and drug resistant cancer cell lines. (C) 2013 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.",
journal = "Phytochemistry Letters",
title = "Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines",
number = "2",
volume = "6",
pages = "1-252",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1016"
}

Fischedick, J. T., Pešić, M., Podolski-Renić, A., Banković, J. Z., de Vos, R. C., Perić, M., Todorović, S.,& Tanić, N. T.. (2013). Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines. in Phytochemistry Letters, 6(2), 1-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1016

Fischedick JT, Pešić M, Podolski-Renić A, Banković JZ, de Vos RC, Perić M, Todorović S, Tanić NT. Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines. in Phytochemistry Letters. 2013;6(2):1-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1016 .

Fischedick, Justin T, Pešić, Milica, Podolski-Renić, Ana, Banković, Jasna Z., de Vos, Ric CH, Perić, Marija, Todorović, Slađana, Tanić, Nikola T, "Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines" in Phytochemistry Letters, 6, no. 2 (2013):1-252,
https://hdl.handle.net/21.15107/rcub_ibiss_1016 .

TY  - JOUR
AU  - Jevtić, Verica V
AU  - Pešić, Milica
AU  - Radić, Gordana P
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan B
AU  - Klisurić, Olivera R
AU  - Podolski-Renić, Ana
AU  - Tanić, Nikola T
AU  - Trifunović, Srecko R
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1010
AB  - The new coumarine derivative, 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, H-1 and C-13 NMR spectroscopy. The proposed structure of the complex was confirmed on the basis of an X-ray structural study. In vitro antitumor activity for the ligand and complex was investigated. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Journal of Molecular Structure
T1  - Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand. Crystal structure of 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one-palladium(II) complex
IS  - null
VL  - 1040
SP  - 5
EP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1010
ER  - 

@article{
author = "Jevtić, Verica V and Pešić, Milica and Radić, Gordana P and Vuković, Nenad and Sukdolak, Slobodan B and Klisurić, Olivera R and Podolski-Renić, Ana and Tanić, Nikola T and Trifunović, Srecko R",
year = "2013",
abstract = "The new coumarine derivative, 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one, and corresponding palladium(II) complex have been synthesized and characterized by microanalysis, infrared, H-1 and C-13 NMR spectroscopy. The proposed structure of the complex was confirmed on the basis of an X-ray structural study. In vitro antitumor activity for the ligand and complex was investigated. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Journal of Molecular Structure",
title = "Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand. Crystal structure of 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one-palladium(II) complex",
number = "null",
volume = "1040",
pages = "5-220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1010"
}

Jevtić, V. V., Pešić, M., Radić, G. P., Vuković, N., Sukdolak, S. B., Klisurić, O. R., Podolski-Renić, A., Tanić, N. T.,& Trifunović, S. R.. (2013). Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand. Crystal structure of 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one-palladium(II) complex. in Journal of Molecular Structure, 1040(null), 5-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1010

Jevtić VV, Pešić M, Radić GP, Vuković N, Sukdolak SB, Klisurić OR, Podolski-Renić A, Tanić NT, Trifunović SR. Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand. Crystal structure of 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one-palladium(II) complex. in Journal of Molecular Structure. 2013;1040(null):5-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1010 .

Jevtić, Verica V, Pešić, Milica, Radić, Gordana P, Vuković, Nenad, Sukdolak, Slobodan B, Klisurić, Olivera R, Podolski-Renić, Ana, Tanić, Nikola T, Trifunović, Srecko R, "Synthesis, characterization and cytotoxicity of a new palladium(II) complex with a coumarin-derived ligand. Crystal structure of 4-hydroxy-3-(1-(p-tolylimino)ethyl)-2H-chromen-2-one-palladium(II) complex" in Journal of Molecular Structure, 1040, no. null (2013):5-220,
https://hdl.handle.net/21.15107/rcub_ibiss_1010 .

TY  - JOUR
AU  - Milinković, Vedrana P.
AU  - Banković, Jasna Z.
AU  - Rakić, Miodrag L
AU  - Stanković, Tijana
AU  - Skender-Gazibara, Milica K
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola T
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/938
AB  - Glioblastoma is the most frequent and malignant human brain tumor. High level of genomic instability detected in glioma cells implies that numerous genetic alterations accumulate during glioma pathogenesis. We investigated alterations in AP-PCR DNA profiles of 30 glioma patients, and detected specific changes in 11 genes not previously associated with this disease: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2, KCNG2, PDE4D, KIR3DL3, and INPP5A. Further correlations revealed that 8 genes might play important role in pathogenesis of glial tumors, while changes in GP2, KCNG2 and KIR3DL3 should be considered as passenger mutations, consequence of high level of genomic instability. Identified genes have a significant role in signal transduction or cell adhesion, which are important processes for cancer development and progression. According to our results, LHFPL3 might be characteristic of primary glioblastoma, SGCG, HTR4, ITGB1, CPS1, PROS1 and INPP5A were detected predominantly in anaplastic astrocytoma, suggesting their role in progression of secondary glioblastoma, while alterations of PDE4D seem to have important role in development of both glioblastoma subtypes. Some of the identified genes showed significant association with p53, p16, and EGFR, but there was no significant correlation between loss of PTEN and any of identified genes. In conclusion our study revealed genetic alterations that were not previously associated with glioma pathogenesis and could be potentially used as molecular markers of different glioblastoma subtypes.
T2  - Plos One
T1  - Identification of Novel Genetic Alterations in Samples of Malignant Glioma Patients
IS  - 12
VL  - 8
SP  - 977
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_938
ER  - 

@article{
author = "Milinković, Vedrana P. and Banković, Jasna Z. and Rakić, Miodrag L and Stanković, Tijana and Skender-Gazibara, Milica K and Ruždijić, Sabera and Tanić, Nikola T",
year = "2013",
abstract = "Glioblastoma is the most frequent and malignant human brain tumor. High level of genomic instability detected in glioma cells implies that numerous genetic alterations accumulate during glioma pathogenesis. We investigated alterations in AP-PCR DNA profiles of 30 glioma patients, and detected specific changes in 11 genes not previously associated with this disease: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2, KCNG2, PDE4D, KIR3DL3, and INPP5A. Further correlations revealed that 8 genes might play important role in pathogenesis of glial tumors, while changes in GP2, KCNG2 and KIR3DL3 should be considered as passenger mutations, consequence of high level of genomic instability. Identified genes have a significant role in signal transduction or cell adhesion, which are important processes for cancer development and progression. According to our results, LHFPL3 might be characteristic of primary glioblastoma, SGCG, HTR4, ITGB1, CPS1, PROS1 and INPP5A were detected predominantly in anaplastic astrocytoma, suggesting their role in progression of secondary glioblastoma, while alterations of PDE4D seem to have important role in development of both glioblastoma subtypes. Some of the identified genes showed significant association with p53, p16, and EGFR, but there was no significant correlation between loss of PTEN and any of identified genes. In conclusion our study revealed genetic alterations that were not previously associated with glioma pathogenesis and could be potentially used as molecular markers of different glioblastoma subtypes.",
journal = "Plos One",
title = "Identification of Novel Genetic Alterations in Samples of Malignant Glioma Patients",
number = "12",
volume = "8",
pages = "977-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_938"
}

Milinković, V. P., Banković, J. Z., Rakić, M. L., Stanković, T., Skender-Gazibara, M. K., Ruždijić, S.,& Tanić, N. T.. (2013). Identification of Novel Genetic Alterations in Samples of Malignant Glioma Patients. in Plos One, 8(12), 977-na.
https://hdl.handle.net/21.15107/rcub_ibiss_938

Milinković VP, Banković JZ, Rakić ML, Stanković T, Skender-Gazibara MK, Ruždijić S, Tanić NT. Identification of Novel Genetic Alterations in Samples of Malignant Glioma Patients. in Plos One. 2013;8(12):977-na.
https://hdl.handle.net/21.15107/rcub_ibiss_938 .

Milinković, Vedrana P., Banković, Jasna Z., Rakić, Miodrag L, Stanković, Tijana, Skender-Gazibara, Milica K, Ruždijić, Sabera, Tanić, Nikola T, "Identification of Novel Genetic Alterations in Samples of Malignant Glioma Patients" in Plos One, 8, no. 12 (2013):977-na,
https://hdl.handle.net/21.15107/rcub_ibiss_938 .

TY  - CONF
AU  - Stojsić, Jelena
AU  - Milinković, Vedrana P.
AU  - Stojković, Sonja
AU  - Dinić, Jelena
AU  - Tanić, Nikola T
AU  - Banković, Jasna Z.
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/975
C3  - European Respiratory Journal
T1  - Loss of heterozygosity of PTEN in patients with non-small cell lung cancer treated and not treated with neoadjuvant chemotherapy
IS  - null
VL  - 42
SP  - 143
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_975
ER  - 

@conference{
author = "Stojsić, Jelena and Milinković, Vedrana P. and Stojković, Sonja and Dinić, Jelena and Tanić, Nikola T and Banković, Jasna Z.",
year = "2013",
journal = "European Respiratory Journal",
title = "Loss of heterozygosity of PTEN in patients with non-small cell lung cancer treated and not treated with neoadjuvant chemotherapy",
number = "null",
volume = "42",
pages = "143-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_975"
}

Stojsić, J., Milinković, V. P., Stojković, S., Dinić, J., Tanić, N. T.,& Banković, J. Z.. (2013). Loss of heterozygosity of PTEN in patients with non-small cell lung cancer treated and not treated with neoadjuvant chemotherapy. in European Respiratory Journal, 42(null), 143-na.
https://hdl.handle.net/21.15107/rcub_ibiss_975

Stojsić J, Milinković VP, Stojković S, Dinić J, Tanić NT, Banković JZ. Loss of heterozygosity of PTEN in patients with non-small cell lung cancer treated and not treated with neoadjuvant chemotherapy. in European Respiratory Journal. 2013;42(null):143-na.
https://hdl.handle.net/21.15107/rcub_ibiss_975 .

Stojsić, Jelena, Milinković, Vedrana P., Stojković, Sonja, Dinić, Jelena, Tanić, Nikola T, Banković, Jasna Z., "Loss of heterozygosity of PTEN in patients with non-small cell lung cancer treated and not treated with neoadjuvant chemotherapy" in European Respiratory Journal, 42, no. null (2013):143-na,
https://hdl.handle.net/21.15107/rcub_ibiss_975 .

TY  - CONF
AU  - Ruždijić, Sabera
AU  - Banković, Jasna Z.
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Milošević, Zorica Z.
AU  - Tanić, Nikola T
AU  - Andra, J
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1161
C3  - European Journal of Cancer
T1  - NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells
IS  - null
VL  - 48
EP  - S225
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1161
ER  - 

@conference{
author = "Ruždijić, Sabera and Banković, Jasna Z. and Podolski-Renić, Ana and Pešić, Milica and Milošević, Zorica Z. and Tanić, Nikola T and Andra, J",
year = "2012",
journal = "European Journal of Cancer",
title = "NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells",
number = "null",
volume = "48",
pages = "S225",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1161"
}

Ruždijić, S., Banković, J. Z., Podolski-Renić, A., Pešić, M., Milošević, Z. Z., Tanić, N. T.,& Andra, J.. (2012). NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells. in European Journal of Cancer, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1161

Ruždijić S, Banković JZ, Podolski-Renić A, Pešić M, Milošević ZZ, Tanić NT, Andra J. NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells. in European Journal of Cancer. 2012;48(null):null-S225.
https://hdl.handle.net/21.15107/rcub_ibiss_1161 .

Ruždijić, Sabera, Banković, Jasna Z., Podolski-Renić, Ana, Pešić, Milica, Milošević, Zorica Z., Tanić, Nikola T, Andra, J, "NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells" in European Journal of Cancer, 48, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1161 .

TY  - CONF
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Chiourea, M
AU  - Banković, Jasna Z.
AU  - Anđelković, Tijana
AU  - Milošević, Zorica Z.
AU  - Milinković, Vedrana P.
AU  - Gagos, S
AU  - Tanić, Nikola T
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1162
C3  - European Journal of Cancer
T1  - Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines
IS  - null
VL  - 48
EP  - S244
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1162
ER  - 

@conference{
author = "Podolski-Renić, Ana and Pešić, Milica and Chiourea, M and Banković, Jasna Z. and Anđelković, Tijana and Milošević, Zorica Z. and Milinković, Vedrana P. and Gagos, S and Tanić, Nikola T",
year = "2012",
journal = "European Journal of Cancer",
title = "Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines",
number = "null",
volume = "48",
pages = "S244",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1162"
}

Podolski-Renić, A., Pešić, M., Chiourea, M., Banković, J. Z., Anđelković, T., Milošević, Z. Z., Milinković, V. P., Gagos, S.,& Tanić, N. T.. (2012). Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines. in European Journal of Cancer, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1162

Podolski-Renić A, Pešić M, Chiourea M, Banković JZ, Anđelković T, Milošević ZZ, Milinković VP, Gagos S, Tanić NT. Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines. in European Journal of Cancer. 2012;48(null):null-S244.
https://hdl.handle.net/21.15107/rcub_ibiss_1162 .

Podolski-Renić, Ana, Pešić, Milica, Chiourea, M, Banković, Jasna Z., Anđelković, Tijana, Milošević, Zorica Z., Milinković, Vedrana P., Gagos, S, Tanić, Nikola T, "Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines" in European Journal of Cancer, 48, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1162 .

TY  - JOUR
AU  - Milinković, Vedrana P.
AU  - Banković, Jasna Z.
AU  - Rakić, Miodrag L
AU  - Milosević, Nebojsa T
AU  - Stanković, Tijana
AU  - Joković, Milos B
AU  - Milošević, Zorica Z.
AU  - Skender-Gazibara, Milica K
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola T
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1108
AB  - The purpose of this study was to detect the level of genomic instability and p53 alterations in anaplastic astrocytoma and primary glioblastoma patients, and to evaluate their impact on glioma pathogenesis and patients outcome. AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal tissue: qualitative changes which represent accumulation of changes in DNA sequence and are the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative changes which represent amplifications or deletions of existing chromosomal material and are the manifestation of chromosomal instability (CIN). Both types of alterations were present in all analyzed samples contributing almost equally to the total level of genomic instability, and showing no differences between histological subtypes. p53 alterations were detected in 40% of samples, predominantly in anaplastic astrocytoma. The higher level of genomic instability was observed in elderly patients (>50 years) and patents with primary glioblastoma. Level of genomic instability had no impact on patients' survival, while presence of p53 alterations seemed to be a favorable prognostic factor in this case. Our results indicate that extensive genomic instability is one of the main features of malignant gliomas. (C) 2012 Elsevier Inc. All rights reserved.
T2  - Experimental and Molecular Pathology
T1  - Genomic instability and p53 alterations in patients with malignant glioma
IS  - 2
VL  - 93
SP  - 67
EP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1108
ER  - 

@article{
author = "Milinković, Vedrana P. and Banković, Jasna Z. and Rakić, Miodrag L and Milosević, Nebojsa T and Stanković, Tijana and Joković, Milos B and Milošević, Zorica Z. and Skender-Gazibara, Milica K and Podolski-Renić, Ana and Pešić, Milica and Ruždijić, Sabera and Tanić, Nikola T",
year = "2012",
abstract = "The purpose of this study was to detect the level of genomic instability and p53 alterations in anaplastic astrocytoma and primary glioblastoma patients, and to evaluate their impact on glioma pathogenesis and patients outcome. AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal tissue: qualitative changes which represent accumulation of changes in DNA sequence and are the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative changes which represent amplifications or deletions of existing chromosomal material and are the manifestation of chromosomal instability (CIN). Both types of alterations were present in all analyzed samples contributing almost equally to the total level of genomic instability, and showing no differences between histological subtypes. p53 alterations were detected in 40% of samples, predominantly in anaplastic astrocytoma. The higher level of genomic instability was observed in elderly patients (>50 years) and patents with primary glioblastoma. Level of genomic instability had no impact on patients' survival, while presence of p53 alterations seemed to be a favorable prognostic factor in this case. Our results indicate that extensive genomic instability is one of the main features of malignant gliomas. (C) 2012 Elsevier Inc. All rights reserved.",
journal = "Experimental and Molecular Pathology",
title = "Genomic instability and p53 alterations in patients with malignant glioma",
number = "2",
volume = "93",
pages = "67-206",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1108"
}

Milinković, V. P., Banković, J. Z., Rakić, M. L., Milosević, N. T., Stanković, T., Joković, M. B., Milošević, Z. Z., Skender-Gazibara, M. K., Podolski-Renić, A., Pešić, M., Ruždijić, S.,& Tanić, N. T.. (2012). Genomic instability and p53 alterations in patients with malignant glioma. in Experimental and Molecular Pathology, 93(2), 67-206.
https://hdl.handle.net/21.15107/rcub_ibiss_1108

Milinković VP, Banković JZ, Rakić ML, Milosević NT, Stanković T, Joković MB, Milošević ZZ, Skender-Gazibara MK, Podolski-Renić A, Pešić M, Ruždijić S, Tanić NT. Genomic instability and p53 alterations in patients with malignant glioma. in Experimental and Molecular Pathology. 2012;93(2):67-206.
https://hdl.handle.net/21.15107/rcub_ibiss_1108 .

Milinković, Vedrana P., Banković, Jasna Z., Rakić, Miodrag L, Milosević, Nebojsa T, Stanković, Tijana, Joković, Milos B, Milošević, Zorica Z., Skender-Gazibara, Milica K, Podolski-Renić, Ana, Pešić, Milica, Ruždijić, Sabera, Tanić, Nikola T, "Genomic instability and p53 alterations in patients with malignant glioma" in Experimental and Molecular Pathology, 93, no. 2 (2012):67-206,
https://hdl.handle.net/21.15107/rcub_ibiss_1108 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Milovanović, Zorka
AU  - Tanić, Nasta
AU  - Džodić, Radan R.
AU  - Juranić, Zorica D
AU  - Susnjar, Snezana
AU  - Plesinac-Karapandžić, Vesna
AU  - Tatić, Svetislav B
AU  - Dramicanin, Tatjana
AU  - Davidović, Radoslav
AU  - Dimitrijević, Bogomir B.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1100
AB  - Tamoxifen is a standard therapeutical treatment in patients with estrogen receptor positive breast carcinoma. However, less than 50% of estrogen receptor positive breast cancers do not respond to tamoxifen treatment whereas 40% of tumors that initially respond to treatment develop resistance over time. The underlying mechanisms for tamoxifen resistance are probably multifactorial but remain largely unknown. The primary aim of this study was to investigate the impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen by analyzing loss of heterozygosity (LOH) and immunohystochemical expression of PTEN in 49 primary breast carcinomas of patients treated with tamoxifen as the only adjuvant therapy. The effect of PTEN inactivation on breast cancer progression and disease outcome was also analyzed. Reduced or completely lost PTEN expression was observed in 55.1% of samples, while 63.3% of samples displayed LOH of PTEN gene. Inactivation of PTEN immunoexpression significantly correlated with the PTEN loss of heterozygosity, suggesting LOH as the most important genetic mechanism for the reduction or complete loss of PTEN expression in primary breast carcinoma. Most importantly, LOH of PTEN and consequential reduction of its immunoexpression showed significant correlation with the recurrence of the disease. Besides, our study revealed that LOH of PTEN tumor suppressor was significantly associated with shorter disease free survival, breast cancer specific survival and overall survival. In summary, our results imply that LOH of PTEN could be used as a good prognostic characteristic for the outcome of breast cancer patients treated with tamoxifen.
T2  - Cancer Biology & Therapy
T1  - The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients
IS  - 12
VL  - 13
SP  - 55
EP  - 1174
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1100
ER  - 

@article{
author = "Tanić, Nikola T and Milovanović, Zorka and Tanić, Nasta and Džodić, Radan R. and Juranić, Zorica D and Susnjar, Snezana and Plesinac-Karapandžić, Vesna and Tatić, Svetislav B and Dramicanin, Tatjana and Davidović, Radoslav and Dimitrijević, Bogomir B.",
year = "2012",
abstract = "Tamoxifen is a standard therapeutical treatment in patients with estrogen receptor positive breast carcinoma. However, less than 50% of estrogen receptor positive breast cancers do not respond to tamoxifen treatment whereas 40% of tumors that initially respond to treatment develop resistance over time. The underlying mechanisms for tamoxifen resistance are probably multifactorial but remain largely unknown. The primary aim of this study was to investigate the impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen by analyzing loss of heterozygosity (LOH) and immunohystochemical expression of PTEN in 49 primary breast carcinomas of patients treated with tamoxifen as the only adjuvant therapy. The effect of PTEN inactivation on breast cancer progression and disease outcome was also analyzed. Reduced or completely lost PTEN expression was observed in 55.1% of samples, while 63.3% of samples displayed LOH of PTEN gene. Inactivation of PTEN immunoexpression significantly correlated with the PTEN loss of heterozygosity, suggesting LOH as the most important genetic mechanism for the reduction or complete loss of PTEN expression in primary breast carcinoma. Most importantly, LOH of PTEN and consequential reduction of its immunoexpression showed significant correlation with the recurrence of the disease. Besides, our study revealed that LOH of PTEN tumor suppressor was significantly associated with shorter disease free survival, breast cancer specific survival and overall survival. In summary, our results imply that LOH of PTEN could be used as a good prognostic characteristic for the outcome of breast cancer patients treated with tamoxifen.",
journal = "Cancer Biology & Therapy",
title = "The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients",
number = "12",
volume = "13",
pages = "55-1174",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1100"
}

Tanić, N. T., Milovanović, Z., Tanić, N., Džodić, R. R., Juranić, Z. D., Susnjar, S., Plesinac-Karapandžić, V., Tatić, S. B., Dramicanin, T., Davidović, R.,& Dimitrijević, B. B.. (2012). The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients. in Cancer Biology & Therapy, 13(12), 55-1174.
https://hdl.handle.net/21.15107/rcub_ibiss_1100

Tanić NT, Milovanović Z, Tanić N, Džodić RR, Juranić ZD, Susnjar S, Plesinac-Karapandžić V, Tatić SB, Dramicanin T, Davidović R, Dimitrijević BB. The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients. in Cancer Biology & Therapy. 2012;13(12):55-1174.
https://hdl.handle.net/21.15107/rcub_ibiss_1100 .

Tanić, Nikola T, Milovanović, Zorka, Tanić, Nasta, Džodić, Radan R., Juranić, Zorica D, Susnjar, Snezana, Plesinac-Karapandžić, Vesna, Tatić, Svetislav B, Dramicanin, Tatjana, Davidović, Radoslav, Dimitrijević, Bogomir B., "The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients" in Cancer Biology & Therapy, 13, no. 12 (2012):55-1174,
https://hdl.handle.net/21.15107/rcub_ibiss_1100 .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Radenković, Miroslav D
AU  - Tanić, Nikola T
AU  - Tanić, Nasta
AU  - Petrović, Nina
AU  - Stojić, Dragica D
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1258
AB  - The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M(3) receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N(G)-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M(3) receptor level.
T2  - European Journal of Oral Sciences
T1  - Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit
IS  - 5
VL  - 119
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1258
ER  - 

@article{
author = "Roganović, Jelena and Radenković, Miroslav D and Tanić, Nikola T and Tanić, Nasta and Petrović, Nina and Stojić, Dragica D",
year = "2011",
abstract = "The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M(3) receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N(G)-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M(3) receptor level.",
journal = "European Journal of Oral Sciences",
title = "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit",
number = "5",
volume = "119",
pages = "360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1258"
}

Roganović, J., Radenković, M. D., Tanić, N. T., Tanić, N., Petrović, N.,& Stojić, D. D.. (2011). Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences, 119(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1258

Roganović J, Radenković MD, Tanić NT, Tanić N, Petrović N, Stojić DD. Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences. 2011;119(5):null-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1258 .

Roganović, Jelena, Radenković, Miroslav D, Tanić, Nikola T, Tanić, Nasta, Petrović, Nina, Stojić, Dragica D, "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit" in European Journal of Oral Sciences, 119, no. 5 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1258 .

TY  - JOUR
AU  - Manitašević-Jovanović, Sanja
AU  - Brkljačić, Jelena
AU  - Matić, Gordana
AU  - Tanić, Nikola T
AU  - Vojnović-Milutinović, Danijela
AU  - Elaković, Ivana
AU  - Perišić, Tatjana
AU  - Dunđerski, Jadranka S.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1388
AB  - Background: Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: beta-actin, glyceraldehyde-3-phosphate dehydrogenase, beta(2)-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD. Results: The variability in expression of the four endogenous controls was assessed by TaqMan Real-time RT-PCR in peripheral blood mononuclear cells from: war veterans with current PTSD, those with lifetime PTSD, trauma controls and healthy subjects. Expression stability was analyzed by GeNorm and NormFinder software packages, and by direct comparison of Ct values. Both, GeNorm and NormFinder identified beta-actin and glyceraldehyde-3-phosphate dehydrogenase as a pair of genes with the lowest stability value. Conclusions: The combination of beta-actin and glyceraldehyde-3-phosphate dehydrogenase appeared to be the most suitable reference for studying alterations in gene expression in peripheral blood mononuclear cells related to vulnerability and resilience to PTSD, as well as to trauma-provoked developing of this disorder and recovery from it. Using glyceraldehyde-3-phosphate dehydrogenase, beta-actin and beta(2)-microglobulin as individual endogenous controls would provide satisfactory data, while RNA polymerase II polypeptide A could not be recommended.
T2  - Bmc Molecular Biology
T1  - Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD
IS  - null
VL  - 11
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1388
ER  - 

@article{
author = "Manitašević-Jovanović, Sanja and Brkljačić, Jelena and Matić, Gordana and Tanić, Nikola T and Vojnović-Milutinović, Danijela and Elaković, Ivana and Perišić, Tatjana and Dunđerski, Jadranka S.",
year = "2010",
abstract = "Background: Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: beta-actin, glyceraldehyde-3-phosphate dehydrogenase, beta(2)-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD. Results: The variability in expression of the four endogenous controls was assessed by TaqMan Real-time RT-PCR in peripheral blood mononuclear cells from: war veterans with current PTSD, those with lifetime PTSD, trauma controls and healthy subjects. Expression stability was analyzed by GeNorm and NormFinder software packages, and by direct comparison of Ct values. Both, GeNorm and NormFinder identified beta-actin and glyceraldehyde-3-phosphate dehydrogenase as a pair of genes with the lowest stability value. Conclusions: The combination of beta-actin and glyceraldehyde-3-phosphate dehydrogenase appeared to be the most suitable reference for studying alterations in gene expression in peripheral blood mononuclear cells related to vulnerability and resilience to PTSD, as well as to trauma-provoked developing of this disorder and recovery from it. Using glyceraldehyde-3-phosphate dehydrogenase, beta-actin and beta(2)-microglobulin as individual endogenous controls would provide satisfactory data, while RNA polymerase II polypeptide A could not be recommended.",
journal = "Bmc Molecular Biology",
title = "Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD",
number = "null",
volume = "11",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1388"
}

Manitašević-Jovanović, S., Brkljačić, J., Matić, G., Tanić, N. T., Vojnović-Milutinović, D., Elaković, I., Perišić, T.,& Dunđerski, J. S.. (2010). Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD. in Bmc Molecular Biology, 11(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1388

Manitašević-Jovanović S, Brkljačić J, Matić G, Tanić NT, Vojnović-Milutinović D, Elaković I, Perišić T, Dunđerski JS. Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD. in Bmc Molecular Biology. 2010;11(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1388 .

Manitašević-Jovanović, Sanja, Brkljačić, Jelena, Matić, Gordana, Tanić, Nikola T, Vojnović-Milutinović, Danijela, Elaković, Ivana, Perišić, Tatjana, Dunđerski, Jadranka S., "Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD" in Bmc Molecular Biology, 11, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1388 .

TY  - JOUR
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Tesić, Vesna T
AU  - Tanić, Nikola T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1414
AB  - Brain aging is related to the numerous structural and functional changes including decreased synaptic plasticity. The beneficial effects of dietary restriction (DR) are well known but insufficiently investigated at the level of plasticity-related markers. Therefore, the aim of this study was to examine the expression profiles of proteins structurally and functionally related to synapses-growth-associated protein 43 (GAP-43), synaptophysin (SPH) and alpha-synuclein (alpha-Syn), in the course of aging and in response to long-term DR. The mRNA and protein levels of three presynaptic proteins were assessed by Real Time RT-PCR and Western blotting in the cortex and hippocampus of young (6-month-old), middle-aged (12-month-old), aged (18-month-old) and old (24-month-old) male Wistar rats fed ad libitum and exposed to DR starting from 6 months of age. We observed that long-term DR modulated age-related transcriptional changes by maintaining stable mRNAs levels in the cortex. No major age-related changes of the protein levels were observed in the cortex, while the specific temporal decline was detected in the hippocampus for all three proteins. The SPH levels were decreased across lifespan (0.8-, 0.8- and 0.6-fold change at 12,18 and 24 months), while the significant decrease of GAP-43 and alpha-Syn protein was detected at 24 months of age (0.6- and 0.7-fold decrease, respectively). Long-term DR eliminated this decline by increasing GAP-43, SPH and alpha-Syn protein levels (1.7-, 1.7- and 1.6-fold, respectively) thus reverting protein levels to the values measured in 6-month-old animals. Specific pattern of changes observed in the hippocampus identifies this structure as more vulnerable to the processes of aging and with a more pronounced response to the DR effects. The observed DR-induced stabilization of the levels of three presynaptic proteins indicates the beneficial effect of DR on age-related decline in the capacity for synaptic plasticity. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat
IS  - 2
VL  - 56
EP  - 255
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1414
ER  - 

@article{
author = "Mladenović, Aleksandra and Perović, Milka and Tesić, Vesna T and Tanić, Nikola T and Rakić, Ljubisav and Ruždijić, Sabera and Kanazir, Selma",
year = "2010",
abstract = "Brain aging is related to the numerous structural and functional changes including decreased synaptic plasticity. The beneficial effects of dietary restriction (DR) are well known but insufficiently investigated at the level of plasticity-related markers. Therefore, the aim of this study was to examine the expression profiles of proteins structurally and functionally related to synapses-growth-associated protein 43 (GAP-43), synaptophysin (SPH) and alpha-synuclein (alpha-Syn), in the course of aging and in response to long-term DR. The mRNA and protein levels of three presynaptic proteins were assessed by Real Time RT-PCR and Western blotting in the cortex and hippocampus of young (6-month-old), middle-aged (12-month-old), aged (18-month-old) and old (24-month-old) male Wistar rats fed ad libitum and exposed to DR starting from 6 months of age. We observed that long-term DR modulated age-related transcriptional changes by maintaining stable mRNAs levels in the cortex. No major age-related changes of the protein levels were observed in the cortex, while the specific temporal decline was detected in the hippocampus for all three proteins. The SPH levels were decreased across lifespan (0.8-, 0.8- and 0.6-fold change at 12,18 and 24 months), while the significant decrease of GAP-43 and alpha-Syn protein was detected at 24 months of age (0.6- and 0.7-fold decrease, respectively). Long-term DR eliminated this decline by increasing GAP-43, SPH and alpha-Syn protein levels (1.7-, 1.7- and 1.6-fold, respectively) thus reverting protein levels to the values measured in 6-month-old animals. Specific pattern of changes observed in the hippocampus identifies this structure as more vulnerable to the processes of aging and with a more pronounced response to the DR effects. The observed DR-induced stabilization of the levels of three presynaptic proteins indicates the beneficial effect of DR on age-related decline in the capacity for synaptic plasticity. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat",
number = "2",
volume = "56",
pages = "255",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1414"
}

Mladenović, A., Perović, M., Tesić, V. T., Tanić, N. T., Rakić, L., Ruždijić, S.,& Kanazir, S.. (2010). Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat. in Neurochemistry International, 56(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1414

Mladenović A, Perović M, Tesić VT, Tanić NT, Rakić L, Ruždijić S, Kanazir S. Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat. in Neurochemistry International. 2010;56(2):null-255.
https://hdl.handle.net/21.15107/rcub_ibiss_1414 .

Mladenović, Aleksandra, Perović, Milka, Tesić, Vesna T, Tanić, Nikola T, Rakić, Ljubisav, Ruždijić, Sabera, Kanazir, Selma, "Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat" in Neurochemistry International, 56, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1414 .

TY  - JOUR
AU  - Perović, Milka
AU  - Mladenović, Aleksandra
AU  - Smiljanić, Kosara
AU  - Tanić, Nikola T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1420
AB  - Expression profiles of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46), proteins involved in cholesterol biosynthesis, transport and excretion from the CNS, were analyzed in the rat cortex, hippocampus and cerebellum as a function of aging (6-24 months) and in response to long-term dietary restriction (DR). Age-related increases for all three mRNAs were observed, with the highest induction found for Cyp46 in the cortex and hippocampus of 24-month-old animals. DR maintained stable levels of Cyp46, HMGR, and ApoE mRNAs during aging, exhibiting an attenuating effect on age-related changes through specific temporal and regional pattern. Neither age nor DR had any prominent effects at the protein level, except for Cyp46 and ApoE protein levels in the hippocampus and cerebellum, respectively. Overall, the changes in the cerebellum were different from those in the cortex and hippocampus. Our results demonstrated a modulatory effect of DR on age-related changes of CYP46, HMGR, and ApoE and suggest that the anti-aging effect of DR is in part mediated though transcriptional modulation of cholesterol metabolism genes in the rat brain.
T2  - Biogerontology
T1  - Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction
IS  - 6
VL  - 10
EP  - 745
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1420
ER  - 

@article{
author = "Perović, Milka and Mladenović, Aleksandra and Smiljanić, Kosara and Tanić, Nikola T and Rakić, Ljubisav and Ruždijić, Sabera and Kanazir, Selma",
year = "2009",
abstract = "Expression profiles of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46), proteins involved in cholesterol biosynthesis, transport and excretion from the CNS, were analyzed in the rat cortex, hippocampus and cerebellum as a function of aging (6-24 months) and in response to long-term dietary restriction (DR). Age-related increases for all three mRNAs were observed, with the highest induction found for Cyp46 in the cortex and hippocampus of 24-month-old animals. DR maintained stable levels of Cyp46, HMGR, and ApoE mRNAs during aging, exhibiting an attenuating effect on age-related changes through specific temporal and regional pattern. Neither age nor DR had any prominent effects at the protein level, except for Cyp46 and ApoE protein levels in the hippocampus and cerebellum, respectively. Overall, the changes in the cerebellum were different from those in the cortex and hippocampus. Our results demonstrated a modulatory effect of DR on age-related changes of CYP46, HMGR, and ApoE and suggest that the anti-aging effect of DR is in part mediated though transcriptional modulation of cholesterol metabolism genes in the rat brain.",
journal = "Biogerontology",
title = "Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction",
number = "6",
volume = "10",
pages = "745",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1420"
}

Perović, M., Mladenović, A., Smiljanić, K., Tanić, N. T., Rakić, L., Ruždijić, S.,& Kanazir, S.. (2009). Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction. in Biogerontology, 10(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1420

Perović M, Mladenović A, Smiljanić K, Tanić NT, Rakić L, Ruždijić S, Kanazir S. Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction. in Biogerontology. 2009;10(6):null-745.
https://hdl.handle.net/21.15107/rcub_ibiss_1420 .

Perović, Milka, Mladenović, Aleksandra, Smiljanić, Kosara, Tanić, Nikola T, Rakić, Ljubisav, Ruždijić, Sabera, Kanazir, Selma, "Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction" in Biogerontology, 10, no. 6 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1420 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Stanojević, Boban R
AU  - Tanić, Nasta
AU  - Schaefer, Stephan
AU  - Niesters, Hubert GM
AU  - Bozić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1424
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
IS  - 2
VL  - 161
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1424
ER  - 

@article{
author = "Tanić, Nikola T and Stanojević, Boban R and Tanić, Nasta and Schaefer, Stephan and Niesters, Hubert GM and Bozić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
number = "2",
volume = "161",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1424"
}

Tanić, N. T., Stanojević, B. R., Tanić, N., Schaefer, S., Niesters, H. G., Bozić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1424

Tanić NT, Stanojević BR, Tanić N, Schaefer S, Niesters HG, Bozić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

Tanić, Nikola T, Stanojević, Boban R, Tanić, Nasta, Schaefer, Stephan, Niesters, Hubert GM, Bozić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

TY  - CONF
AU  - Banković, Jasna Z.
AU  - Stojsić, Jelena M
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola T
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1431
C3  - Ejc Supplements
T1  - Identification of altered genes associated with non-small cell lung cancer promotion and progression
IS  - 2
VL  - 7
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1431
ER  - 

@conference{
author = "Banković, Jasna Z. and Stojsić, Jelena M and Ruždijić, Sabera and Tanić, Nikola T",
year = "2009",
journal = "Ejc Supplements",
title = "Identification of altered genes associated with non-small cell lung cancer promotion and progression",
number = "2",
volume = "7",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1431"
}

Banković, J. Z., Stojsić, J. M., Ruždijić, S.,& Tanić, N. T.. (2009). Identification of altered genes associated with non-small cell lung cancer promotion and progression. in Ejc Supplements, 7(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1431

Banković JZ, Stojsić JM, Ruždijić S, Tanić NT. Identification of altered genes associated with non-small cell lung cancer promotion and progression. in Ejc Supplements. 2009;7(2):null-110.
https://hdl.handle.net/21.15107/rcub_ibiss_1431 .

Banković, Jasna Z., Stojsić, Jelena M, Ruždijić, Sabera, Tanić, Nikola T, "Identification of altered genes associated with non-small cell lung cancer promotion and progression" in Ejc Supplements, 7, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1431 .

TY  - JOUR
AU  - Tanić, Nasta
AU  - Tanić, Nikola T
AU  - Milasin, Jelena M
AU  - Vukadinović, Miroslav
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1448
AB  - Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.
T2  - European Journal of Oral Sciences
T1  - Genomic instability and tumor-specific DNA alterations in oral leukoplakias
IS  - 3
VL  - 117
EP  - 237
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1448
ER  - 

@article{
author = "Tanić, Nasta and Tanić, Nikola T and Milasin, Jelena M and Vukadinović, Miroslav and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.",
journal = "European Journal of Oral Sciences",
title = "Genomic instability and tumor-specific DNA alterations in oral leukoplakias",
number = "3",
volume = "117",
pages = "237",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1448"
}

Tanić, N., Tanić, N. T., Milasin, J. M., Vukadinović, M.,& Dimitrijević, B. B.. (2009). Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences, 117(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1448

Tanić N, Tanić NT, Milasin JM, Vukadinović M, Dimitrijević BB. Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences. 2009;117(3):null-237.
https://hdl.handle.net/21.15107/rcub_ibiss_1448 .

Tanić, Nasta, Tanić, Nikola T, Milasin, Jelena M, Vukadinović, Miroslav, Dimitrijević, Bogomir B., "Genomic instability and tumor-specific DNA alterations in oral leukoplakias" in European Journal of Oral Sciences, 117, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1448 .

TY  - CONF
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Tanić, Nikola T
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1521
C3  - European Neuropsychopharmacology
T1  - Dietary restriction effects on age induced changes in APP, PS-1 and ADAM mRNA expression
IS  - null
VL  - 18
EP  - S519
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1521
ER  - 

@conference{
author = "Mladenović, Aleksandra and Perović, Milka and Tanić, Nikola T and Ruždijić, Sabera and Kanazir, Selma",
year = "2008",
journal = "European Neuropsychopharmacology",
title = "Dietary restriction effects on age induced changes in APP, PS-1 and ADAM mRNA expression",
number = "null",
volume = "18",
pages = "S519",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1521"
}

Mladenović, A., Perović, M., Tanić, N. T., Ruždijić, S.,& Kanazir, S.. (2008). Dietary restriction effects on age induced changes in APP, PS-1 and ADAM mRNA expression. in European Neuropsychopharmacology, 18(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1521

Mladenović A, Perović M, Tanić NT, Ruždijić S, Kanazir S. Dietary restriction effects on age induced changes in APP, PS-1 and ADAM mRNA expression. in European Neuropsychopharmacology. 2008;18(null):null-S519.
https://hdl.handle.net/21.15107/rcub_ibiss_1521 .

Mladenović, Aleksandra, Perović, Milka, Tanić, Nikola T, Ruždijić, Sabera, Kanazir, Selma, "Dietary restriction effects on age induced changes in APP, PS-1 and ADAM mRNA expression" in European Neuropsychopharmacology, 18, no. null (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1521 .

TY  - JOUR
AU  - Gavrilović, Ljubica V
AU  - Spasojević, Natasa
AU  - Tanić, Nikola T
AU  - Dronjak, Slađana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1504
AB  - OBJECTIVE: Isolation of adult animals represents a form of psychsocial stress that produces sympatho-adrenomedullar activation. The aim of this work was to investigate the changes in gene expression and protein levels of catecholamine biosynthetic enzymes: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in the adrenal medulla of naive control and chronically (12 weeks) socially isolated adult Wistar rat males and the response of these animals to additional immobilization stress (2 h). METHODS: TH, DBH and PNMT mRNA levels were quantified by quantitative real-time RT-PCR (qRT-PCR). TH, DBH and PNMT immunoproteins were assayed by Western Blot. RESULTS: In chronically isolated rats, gene expression levels of catecholamine biosynthetic enzymes in the adrenal medulla were decreased, but only TH mRNA was significantly decreased. However, protein levels of TH, DBH and PNMT of these animals were elevated by 55%, 20% and 18%, respectively, in relation to the corresponding control. Naive control and chronically socially isolated rats exposed to additional 2-h-immobilization showed increased gene expression of the examined enzymes, the increase being greater in socially isolated rats as compared to the controls. Additional immobilization of naive controls did not affect TH, DBH and PNMT protein levels. In contrast, this stress produced increased TH, DBH and PNMT protein levels in long-term socially isolated rats. CONCLUSION: We can conclud that psychosocial stress expressed a differential influence on gene expression and protein levels of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats. The results indicate a possible adaptation of catecholamine-synthesizing system at the level of TH gene expression in adrenal medulla of chronically isolated animals.
T2  - Neuroendocrinology Letters
T1  - Chronic isolation of adult rats decreases gene expression of catecholamine biosynthetic enzymes in adrenal medulla
IS  - 6
VL  - 29
EP  - 1020
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1504
ER  - 

@article{
author = "Gavrilović, Ljubica V and Spasojević, Natasa and Tanić, Nikola T and Dronjak, Slađana",
year = "2008",
abstract = "OBJECTIVE: Isolation of adult animals represents a form of psychsocial stress that produces sympatho-adrenomedullar activation. The aim of this work was to investigate the changes in gene expression and protein levels of catecholamine biosynthetic enzymes: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in the adrenal medulla of naive control and chronically (12 weeks) socially isolated adult Wistar rat males and the response of these animals to additional immobilization stress (2 h). METHODS: TH, DBH and PNMT mRNA levels were quantified by quantitative real-time RT-PCR (qRT-PCR). TH, DBH and PNMT immunoproteins were assayed by Western Blot. RESULTS: In chronically isolated rats, gene expression levels of catecholamine biosynthetic enzymes in the adrenal medulla were decreased, but only TH mRNA was significantly decreased. However, protein levels of TH, DBH and PNMT of these animals were elevated by 55%, 20% and 18%, respectively, in relation to the corresponding control. Naive control and chronically socially isolated rats exposed to additional 2-h-immobilization showed increased gene expression of the examined enzymes, the increase being greater in socially isolated rats as compared to the controls. Additional immobilization of naive controls did not affect TH, DBH and PNMT protein levels. In contrast, this stress produced increased TH, DBH and PNMT protein levels in long-term socially isolated rats. CONCLUSION: We can conclud that psychosocial stress expressed a differential influence on gene expression and protein levels of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats. The results indicate a possible adaptation of catecholamine-synthesizing system at the level of TH gene expression in adrenal medulla of chronically isolated animals.",
journal = "Neuroendocrinology Letters",
title = "Chronic isolation of adult rats decreases gene expression of catecholamine biosynthetic enzymes in adrenal medulla",
number = "6",
volume = "29",
pages = "1020",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1504"
}

Gavrilović, L. V., Spasojević, N., Tanić, N. T.,& Dronjak, S.. (2008). Chronic isolation of adult rats decreases gene expression of catecholamine biosynthetic enzymes in adrenal medulla. in Neuroendocrinology Letters, 29(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1504

Gavrilović LV, Spasojević N, Tanić NT, Dronjak S. Chronic isolation of adult rats decreases gene expression of catecholamine biosynthetic enzymes in adrenal medulla. in Neuroendocrinology Letters. 2008;29(6):null-1020.
https://hdl.handle.net/21.15107/rcub_ibiss_1504 .

Gavrilović, Ljubica V, Spasojević, Natasa, Tanić, Nikola T, Dronjak, Slađana, "Chronic isolation of adult rats decreases gene expression of catecholamine biosynthetic enzymes in adrenal medulla" in Neuroendocrinology Letters, 29, no. 6 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1504 .

TY  - JOUR
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Tanić, Nikola T
AU  - Petanceska, Suzana
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1584
AB  - Dietary restriction (DR) is one of the promising environmental interventions known to attenuate aging and decrease risk of age-related neurodegenerative disorders. The aim of this study was to assess the effects of DR on expression of a-synuclein, a presynaptic protein involved in pathogenesis of Parkinson's and some other neurodegenerative diseases, in the cortex and hippocampus of adult, middle-aged, late middle-aged, and aged rats. Using Real Time RT-PCR, the authors report that aging regulates the expression of alpha-synuclein in a tissue-specific manner and that long-term DR reverts the late age-related changes of alpha-synuclein expression. (c) 2007 Wiley-Liss, Inc.
T2  - Synapse
T1  - Dietary restriction modulates alpha-synuclein expression in the aging rat cortex and hippocampus
IS  - 9
VL  - 61
EP  - 794
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1584
ER  - 

@article{
author = "Mladenović, Aleksandra and Perović, Milka and Tanić, Nikola T and Petanceska, Suzana and Ruždijić, Sabera and Kanazir, Selma",
year = "2007",
abstract = "Dietary restriction (DR) is one of the promising environmental interventions known to attenuate aging and decrease risk of age-related neurodegenerative disorders. The aim of this study was to assess the effects of DR on expression of a-synuclein, a presynaptic protein involved in pathogenesis of Parkinson's and some other neurodegenerative diseases, in the cortex and hippocampus of adult, middle-aged, late middle-aged, and aged rats. Using Real Time RT-PCR, the authors report that aging regulates the expression of alpha-synuclein in a tissue-specific manner and that long-term DR reverts the late age-related changes of alpha-synuclein expression. (c) 2007 Wiley-Liss, Inc.",
journal = "Synapse",
title = "Dietary restriction modulates alpha-synuclein expression in the aging rat cortex and hippocampus",
number = "9",
volume = "61",
pages = "794",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1584"
}

Mladenović, A., Perović, M., Tanić, N. T., Petanceska, S., Ruždijić, S.,& Kanazir, S.. (2007). Dietary restriction modulates alpha-synuclein expression in the aging rat cortex and hippocampus. in Synapse, 61(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1584

Mladenović A, Perović M, Tanić NT, Petanceska S, Ruždijić S, Kanazir S. Dietary restriction modulates alpha-synuclein expression in the aging rat cortex and hippocampus. in Synapse. 2007;61(9):null-794.
https://hdl.handle.net/21.15107/rcub_ibiss_1584 .

Mladenović, Aleksandra, Perović, Milka, Tanić, Nikola T, Petanceska, Suzana, Ruždijić, Sabera, Kanazir, Selma, "Dietary restriction modulates alpha-synuclein expression in the aging rat cortex and hippocampus" in Synapse, 61, no. 9 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1584 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Perović, Milka
AU  - Mladenović, Aleksandra
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1616
AB  - Accurate normalization is the prerequisite for obtaining reliable results in the quantification of gene expression. Using TaqMan Real Time RT-PCR, we carried out an extensive evaluation of five most commonly used endogenous controls, gapdh, beta-actin, 18S rRNA, hprt and cypB, for their presumed stability of expression, in rat cortex and hippocampus, during aging, under dietary restriction and dexamethasone treatment. Valid reference genes (HKGs) were identified using GeNorm and NormFinder software packages and by direct comparison of Ct values. Analysis revealed gapdh and P-actin as the most stable HKGs for all treatments analyzed, combined or separately, in the cortex, while in the hippocampus gapdh/ hprt and beta-actin/hprt are the combination of choice for the single or combined effects of dietary restriction/dexamethasone, respectively. All treatments significantly influenced expression of 18S rRNA and cypB in both structures. In addition, we used gapdh and normalization factor, calculated by GeNorm, to compare the expression of a-syn in the cortex. Our results demonstrate the importance of the right choice of HKG and suggest the appropriate endogenous control to be used for TaqMan RT-PCR analysis of mRNA expression in rat cortex and hippocampus for selected experimental paradigms.
T2  - Journal of Molecular Neuroscience
T1  - Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus - Evaluation by real time RT-PCR
IS  - 1
VL  - 32
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1616
ER  - 

@article{
author = "Tanić, Nikola T and Perović, Milka and Mladenović, Aleksandra and Ruždijić, Sabera and Kanazir, Selma",
year = "2007",
abstract = "Accurate normalization is the prerequisite for obtaining reliable results in the quantification of gene expression. Using TaqMan Real Time RT-PCR, we carried out an extensive evaluation of five most commonly used endogenous controls, gapdh, beta-actin, 18S rRNA, hprt and cypB, for their presumed stability of expression, in rat cortex and hippocampus, during aging, under dietary restriction and dexamethasone treatment. Valid reference genes (HKGs) were identified using GeNorm and NormFinder software packages and by direct comparison of Ct values. Analysis revealed gapdh and P-actin as the most stable HKGs for all treatments analyzed, combined or separately, in the cortex, while in the hippocampus gapdh/ hprt and beta-actin/hprt are the combination of choice for the single or combined effects of dietary restriction/dexamethasone, respectively. All treatments significantly influenced expression of 18S rRNA and cypB in both structures. In addition, we used gapdh and normalization factor, calculated by GeNorm, to compare the expression of a-syn in the cortex. Our results demonstrate the importance of the right choice of HKG and suggest the appropriate endogenous control to be used for TaqMan RT-PCR analysis of mRNA expression in rat cortex and hippocampus for selected experimental paradigms.",
journal = "Journal of Molecular Neuroscience",
title = "Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus - Evaluation by real time RT-PCR",
number = "1",
volume = "32",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1616"
}

Tanić, N. T., Perović, M., Mladenović, A., Ruždijić, S.,& Kanazir, S.. (2007). Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus - Evaluation by real time RT-PCR. in Journal of Molecular Neuroscience, 32(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1616

Tanić NT, Perović M, Mladenović A, Ruždijić S, Kanazir S. Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus - Evaluation by real time RT-PCR. in Journal of Molecular Neuroscience. 2007;32(1):null-46.
https://hdl.handle.net/21.15107/rcub_ibiss_1616 .

Tanić, Nikola T, Perović, Milka, Mladenović, Aleksandra, Ruždijić, Sabera, Kanazir, Selma, "Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus - Evaluation by real time RT-PCR" in Journal of Molecular Neuroscience, 32, no. 1 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1616 .

TY  - CONF
AU  - Anđelković, Tijana
AU  - Pešić, Milica
AU  - Banković, Jasna Z.
AU  - Tanić, Nikola T
AU  - Ruždijić, Sabera
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1580
C3  - Ejc Supplements
T1  - Effect of sulfinosine [(R,S)-2-amino-9-beta-D-ribofuranosylpurine-6-sulfinamide] on lung carcinoma cell lines and its role in overcoming multidrug resistance
IS  - 4
VL  - 5
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1580
ER  - 

@conference{
author = "Anđelković, Tijana and Pešić, Milica and Banković, Jasna Z. and Tanić, Nikola T and Ruždijić, Sabera",
year = "2007",
journal = "Ejc Supplements",
title = "Effect of sulfinosine [(R,S)-2-amino-9-beta-D-ribofuranosylpurine-6-sulfinamide] on lung carcinoma cell lines and its role in overcoming multidrug resistance",
number = "4",
volume = "5",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1580"
}

Anđelković, T., Pešić, M., Banković, J. Z., Tanić, N. T.,& Ruždijić, S.. (2007). Effect of sulfinosine [(R,S)-2-amino-9-beta-D-ribofuranosylpurine-6-sulfinamide] on lung carcinoma cell lines and its role in overcoming multidrug resistance. in Ejc Supplements, 5(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1580

Anđelković T, Pešić M, Banković JZ, Tanić NT, Ruždijić S. Effect of sulfinosine [(R,S)-2-amino-9-beta-D-ribofuranosylpurine-6-sulfinamide] on lung carcinoma cell lines and its role in overcoming multidrug resistance. in Ejc Supplements. 2007;5(4):null-71.
https://hdl.handle.net/21.15107/rcub_ibiss_1580 .

Anđelković, Tijana, Pešić, Milica, Banković, Jasna Z., Tanić, Nikola T, Ruždijić, Sabera, "Effect of sulfinosine [(R,S)-2-amino-9-beta-D-ribofuranosylpurine-6-sulfinamide] on lung carcinoma cell lines and its role in overcoming multidrug resistance" in Ejc Supplements, 5, no. 4 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1580 .

TY  - JOUR
AU  - Mandušić, Vesna
AU  - Nikolić-Vukosavljević, Dragica
AU  - Tanić, Nikola T
AU  - Kanjer, Ksenija
AU  - Nešković-Konstantinović, Zora B.
AU  - Celeketić, Dusica C
AU  - Dimitrijević, Bogomir B.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1586
AB  - Purpose In addition to Estrogen Receptor alpha (ER alpha) and Progesterone Receptor (PR), the Second Estrogen Receptor (ER beta) appears to play an important role not only in estrogen signaling, but also in the pathogenesis of cancer in estrogen dependent tissues. The existence of various isoforms and splice variants of both ERs additionally complicates elucidation of their physiological role and involvement in the process of carcinogenesis. Methods In this study, the expression of ER beta 1 mRNA (wild type of beta receptor) and splice variant ER beta Delta 5 mRNA (which codes for truncated protein) was measured by the quantitative RT-PCR (q RT-PCR) in the 60 samples of Breast Cancer (BC) and correlated with ER alpha and PR protein levels and with clinical and histopathological parameters. Results We found the inverse correlation of ER beta Delta 5 mRNA expression with the levels of PR and ER alpha proteins in the group of postmenopausal patients; we also report the lower expression of ER beta 1 and ER beta Delta 5 mRNA in the larger tumors (>20 mm, T2, and T3) than in smaller ones (<= 20 mm, T1). The decrease of ER beta Delta 5 mRNA expression in larger tumors is found to arise from ER-positive breast carcinomas. In addition, the portion of tumors with concomitant high expression of both transcripts matches up the known percentage of tumors resistant to endocrine therapy in patients with different ER/PR status. Conclusions As far as we know, this is the first study in which ER beta Delta 5 mRNA splice variant was quantified by realtime RT-PCR in the clinical samples of breast cancer tissue. Until now, the focus of clinical reports was the level of ER beta 1, ER beta 2, and ER beta 5 isoforms. The higher expression of ER beta Delta 5 mRNA is associated with the indicators of low biological aggressiveness of tumor (low tumor size within ER-positive status in our study) suggesting that the uncontrolled local tumor growth may occur as the expression of ER beta Delta 5 mRNA decreases in estrogen-dependent breast cancer.
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer
IS  - 8
VL  - 133
EP  - 579
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1586
ER  - 

@article{
author = "Mandušić, Vesna and Nikolić-Vukosavljević, Dragica and Tanić, Nikola T and Kanjer, Ksenija and Nešković-Konstantinović, Zora B. and Celeketić, Dusica C and Dimitrijević, Bogomir B.",
year = "2007",
abstract = "Purpose In addition to Estrogen Receptor alpha (ER alpha) and Progesterone Receptor (PR), the Second Estrogen Receptor (ER beta) appears to play an important role not only in estrogen signaling, but also in the pathogenesis of cancer in estrogen dependent tissues. The existence of various isoforms and splice variants of both ERs additionally complicates elucidation of their physiological role and involvement in the process of carcinogenesis. Methods In this study, the expression of ER beta 1 mRNA (wild type of beta receptor) and splice variant ER beta Delta 5 mRNA (which codes for truncated protein) was measured by the quantitative RT-PCR (q RT-PCR) in the 60 samples of Breast Cancer (BC) and correlated with ER alpha and PR protein levels and with clinical and histopathological parameters. Results We found the inverse correlation of ER beta Delta 5 mRNA expression with the levels of PR and ER alpha proteins in the group of postmenopausal patients; we also report the lower expression of ER beta 1 and ER beta Delta 5 mRNA in the larger tumors (>20 mm, T2, and T3) than in smaller ones (<= 20 mm, T1). The decrease of ER beta Delta 5 mRNA expression in larger tumors is found to arise from ER-positive breast carcinomas. In addition, the portion of tumors with concomitant high expression of both transcripts matches up the known percentage of tumors resistant to endocrine therapy in patients with different ER/PR status. Conclusions As far as we know, this is the first study in which ER beta Delta 5 mRNA splice variant was quantified by realtime RT-PCR in the clinical samples of breast cancer tissue. Until now, the focus of clinical reports was the level of ER beta 1, ER beta 2, and ER beta 5 isoforms. The higher expression of ER beta Delta 5 mRNA is associated with the indicators of low biological aggressiveness of tumor (low tumor size within ER-positive status in our study) suggesting that the uncontrolled local tumor growth may occur as the expression of ER beta Delta 5 mRNA decreases in estrogen-dependent breast cancer.",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer",
number = "8",
volume = "133",
pages = "579",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1586"
}

Mandušić, V., Nikolić-Vukosavljević, D., Tanić, N. T., Kanjer, K., Nešković-Konstantinović, Z. B., Celeketić, D. C.,& Dimitrijević, B. B.. (2007). Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer. in Journal of Cancer Research and Clinical Oncology, 133(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1586

Mandušić V, Nikolić-Vukosavljević D, Tanić NT, Kanjer K, Nešković-Konstantinović ZB, Celeketić DC, Dimitrijević BB. Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer. in Journal of Cancer Research and Clinical Oncology. 2007;133(8):null-579.
https://hdl.handle.net/21.15107/rcub_ibiss_1586 .

Mandušić, Vesna, Nikolić-Vukosavljević, Dragica, Tanić, Nikola T, Kanjer, Ksenija, Nešković-Konstantinović, Zora B., Celeketić, Dusica C, Dimitrijević, Bogomir B., "Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer" in Journal of Cancer Research and Clinical Oncology, 133, no. 8 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1586 .

TY  - CONF
AU  - Banković, Jasna Z.
AU  - Stojsić, Jelena M
AU  - Zunić, Svetlana S
AU  - Selaković, I
AU  - Paunović, V
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola T
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1581
C3  - Ejc Supplements
T1  - Genomic instability in non-small cell lung cancer assessed by arbitrarily primed polymerase chain reaction
IS  - 4
VL  - 5
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1581
ER  - 

@conference{
author = "Banković, Jasna Z. and Stojsić, Jelena M and Zunić, Svetlana S and Selaković, I and Paunović, V and Ruždijić, Sabera and Tanić, Nikola T",
year = "2007",
journal = "Ejc Supplements",
title = "Genomic instability in non-small cell lung cancer assessed by arbitrarily primed polymerase chain reaction",
number = "4",
volume = "5",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1581"
}

Banković, J. Z., Stojsić, J. M., Zunić, S. S., Selaković, I., Paunović, V., Ruždijić, S.,& Tanić, N. T.. (2007). Genomic instability in non-small cell lung cancer assessed by arbitrarily primed polymerase chain reaction. in Ejc Supplements, 5(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1581

Banković JZ, Stojsić JM, Zunić SS, Selaković I, Paunović V, Ruždijić S, Tanić NT. Genomic instability in non-small cell lung cancer assessed by arbitrarily primed polymerase chain reaction. in Ejc Supplements. 2007;5(4):null-74.
https://hdl.handle.net/21.15107/rcub_ibiss_1581 .

Banković, Jasna Z., Stojsić, Jelena M, Zunić, Svetlana S, Selaković, I, Paunović, V, Ruždijić, Sabera, Tanić, Nikola T, "Genomic instability in non-small cell lung cancer assessed by arbitrarily primed polymerase chain reaction" in Ejc Supplements, 5, no. 4 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1581 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Vujošević, Mladen
AU  - Dedović-Tanić, Nasta
AU  - Dimitrijević, Bogomir B.
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1706
AB  - Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.
T2  - Chromosoma
T1  - Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes
IS  - 8
VL  - 113
EP  - 427
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1706
ER  - 

@article{
author = "Tanić, Nikola T and Vujošević, Mladen and Dedović-Tanić, Nasta and Dimitrijević, Bogomir B.",
year = "2005",
abstract = "Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.",
journal = "Chromosoma",
title = "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes",
number = "8",
volume = "113",
pages = "427",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1706"
}

Tanić, N. T., Vujošević, M., Dedović-Tanić, N.,& Dimitrijević, B. B.. (2005). Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma, 113(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1706

Tanić NT, Vujošević M, Dedović-Tanić N, Dimitrijević BB. Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma. 2005;113(8):null-427.
https://hdl.handle.net/21.15107/rcub_ibiss_1706 .

Tanić, Nikola T, Vujošević, Mladen, Dedović-Tanić, Nasta, Dimitrijević, Bogomir B., "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes" in Chromosoma, 113, no. 8 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1706 .