TY  - CONF
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Stevanović, Katarina
AU  - Pajić, Tanja
AU  - Cvetić Antić, Tijana
AU  - Atlagić, Kristina
AU  - Krmpot, Aleksandar
PY  - 2023
UR  - https://microbiology.bg.ac.rs/workshop2023/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6248
AB  - The use of chemical fungicides is detrimental for soil and aquatic ecosystems.
Although highly effective, they raise concerns about impact on the environment.
Therefore, the need to reduce the use of chemical fungicides and protect
the soil and water, pose the challenge to find novel, eco-friendly, but also
more efficient, means of crop protection from phytopathogenic fungi. We are
aiming to utilize the native Trichoderma species, as biological control agent,
for controlling or eliminating the phytopathogenic fungi. Our research will be
based on cell nano-surgery of fungal cell wall using ultrashort laser pulses and
subsequent patch clamping on the released protoplast membrane. This will
enable the studies of activity and potency of specific class of antimicrobial short
peptides, peptaibols, released by Trichoderma species, which act by forming
ion channels in pathogen membrane as one of the main mechanisms of their
fungicide action. The result of this project will be the developed instrumentation
and unique method based on nano-surgery and patch clamp for studies of ionic
channels in native fungal plasma membrane. Testing a range of autochthonous
Trichoderma sp. isolates will enable formulation of their combinations that work
best. The selected Trichoderma strains with the highest standardized peptaibol
activity in specific combination of conditions, can be used as a starting point for
development of new, more potent biocontrol agents.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: ICGEB Workshop: Trends in microbial solutions for sustainable agriculture; 2023 Sep13-15; Belgrade, Serbia
T1  - Novel research project – BioPhysFun for advancement of characterization of Trichoderma as biological control agent
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6248
ER  - 

@conference{
author = "Todorović, Nataša and Živić, Miroslav and Stevanović, Katarina and Pajić, Tanja and Cvetić Antić, Tijana and Atlagić, Kristina and Krmpot, Aleksandar",
year = "2023",
abstract = "The use of chemical fungicides is detrimental for soil and aquatic ecosystems.
Although highly effective, they raise concerns about impact on the environment.
Therefore, the need to reduce the use of chemical fungicides and protect
the soil and water, pose the challenge to find novel, eco-friendly, but also
more efficient, means of crop protection from phytopathogenic fungi. We are
aiming to utilize the native Trichoderma species, as biological control agent,
for controlling or eliminating the phytopathogenic fungi. Our research will be
based on cell nano-surgery of fungal cell wall using ultrashort laser pulses and
subsequent patch clamping on the released protoplast membrane. This will
enable the studies of activity and potency of specific class of antimicrobial short
peptides, peptaibols, released by Trichoderma species, which act by forming
ion channels in pathogen membrane as one of the main mechanisms of their
fungicide action. The result of this project will be the developed instrumentation
and unique method based on nano-surgery and patch clamp for studies of ionic
channels in native fungal plasma membrane. Testing a range of autochthonous
Trichoderma sp. isolates will enable formulation of their combinations that work
best. The selected Trichoderma strains with the highest standardized peptaibol
activity in specific combination of conditions, can be used as a starting point for
development of new, more potent biocontrol agents.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: ICGEB Workshop: Trends in microbial solutions for sustainable agriculture; 2023 Sep13-15; Belgrade, Serbia",
title = "Novel research project – BioPhysFun for advancement of characterization of Trichoderma as biological control agent",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6248"
}

Todorović, N., Živić, M., Stevanović, K., Pajić, T., Cvetić Antić, T., Atlagić, K.,& Krmpot, A.. (2023). Novel research project – BioPhysFun for advancement of characterization of Trichoderma as biological control agent. in Book of Abstracts: ICGEB Workshop: Trends in microbial solutions for sustainable agriculture; 2023 Sep13-15; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 84.
https://hdl.handle.net/21.15107/rcub_ibiss_6248

Todorović N, Živić M, Stevanović K, Pajić T, Cvetić Antić T, Atlagić K, Krmpot A. Novel research project – BioPhysFun for advancement of characterization of Trichoderma as biological control agent. in Book of Abstracts: ICGEB Workshop: Trends in microbial solutions for sustainable agriculture; 2023 Sep13-15; Belgrade, Serbia. 2023;:84.
https://hdl.handle.net/21.15107/rcub_ibiss_6248 .

Todorović, Nataša, Živić, Miroslav, Stevanović, Katarina, Pajić, Tanja, Cvetić Antić, Tijana, Atlagić, Kristina, Krmpot, Aleksandar, "Novel research project – BioPhysFun for advancement of characterization of Trichoderma as biological control agent" in Book of Abstracts: ICGEB Workshop: Trends in microbial solutions for sustainable agriculture; 2023 Sep13-15; Belgrade, Serbia (2023):84,
https://hdl.handle.net/21.15107/rcub_ibiss_6248 .

TY  - CONF
AU  - Pajić, Tanja
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Rabasović, Mihailo
AU  - Krmpot, Aleksandar
PY  - 2022
UR  - http://www.photonicsworkshop.ipb.ac.rs/15/images/RFotonika2022KnjigaApstrakata-B5-CIP.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5735
AB  - Lipids in oleaginous filamentous fungi are considered to be a valuable alternative resource for various biotechnological applications. In vivo label-free imaging enables monitoring of fungi lipid droplets (LD) accumulation with the minimal unwanted effects on the metabolism. LDs are the main source of contrast [1] in the Third Harmonic Generation (THG) microscopy method [2] due to their optical properties (high refractive index). The THG phenomenon is utilized in nonlinear laser scanning microscopy that employs ultra-short laser pulses for imaging. We present in vivo and label-free THG imaging of the individual hyphae of Phycomyces blakesleeanus (Figure 1), an oleaginous filamentous fungus with very rapid growth rate. The THG signal was detected in the forward direction (transmission arm) by PMT through Hoya glass UV filter with peak transmission at 340 nm. The Yb KGW laser, wavelength 1040 nm, pulse duration 200 fs, and repetition rate 83 MHz, has been the source  of the infrared femtosecond pulses. The LDs from THG images were quantified by two image analysis techniques: Image Correlation Spectroscopy (ICS) and software particle counting Particle Size Analysis (PSA). We used hyphae that undergo nitrogen starvation, which is known to cause alterations in lipid metabolism and increase of cellular lipid reserve. The two analysis methods gave similar results. The applicability of the described procedure can be easily extended to other unicellular organisms for the in vivo quantification of LDs since there is no need for sample labeling, fixation or any other specific preparation. In addition, we demonstrate that the ICA is suitable for THG images, although it is primarily developed and have been mostly used for fluorescence signals so far. REFERENCES [1] D. Débarre, W. Supatto et al., Nat. Methods, 3(1), (2006), 47-53. [2] R. W. Boyd, Nonlinear Optics, Academic Press (2008).
PB  - Belgrade: Institute of Physics
C3  - Book of Abstracts: 15th Photonics Workshop: Conference; 2022 Mar 13-16; Kopaonik, Serbia
T1  - Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi
SP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5735
ER  - 

@conference{
author = "Pajić, Tanja and Todorović, Nataša and Živić, Miroslav and Rabasović, Mihailo and Krmpot, Aleksandar",
year = "2022",
abstract = "Lipids in oleaginous filamentous fungi are considered to be a valuable alternative resource for various biotechnological applications. In vivo label-free imaging enables monitoring of fungi lipid droplets (LD) accumulation with the minimal unwanted effects on the metabolism. LDs are the main source of contrast [1] in the Third Harmonic Generation (THG) microscopy method [2] due to their optical properties (high refractive index). The THG phenomenon is utilized in nonlinear laser scanning microscopy that employs ultra-short laser pulses for imaging. We present in vivo and label-free THG imaging of the individual hyphae of Phycomyces blakesleeanus (Figure 1), an oleaginous filamentous fungus with very rapid growth rate. The THG signal was detected in the forward direction (transmission arm) by PMT through Hoya glass UV filter with peak transmission at 340 nm. The Yb KGW laser, wavelength 1040 nm, pulse duration 200 fs, and repetition rate 83 MHz, has been the source  of the infrared femtosecond pulses. The LDs from THG images were quantified by two image analysis techniques: Image Correlation Spectroscopy (ICS) and software particle counting Particle Size Analysis (PSA). We used hyphae that undergo nitrogen starvation, which is known to cause alterations in lipid metabolism and increase of cellular lipid reserve. The two analysis methods gave similar results. The applicability of the described procedure can be easily extended to other unicellular organisms for the in vivo quantification of LDs since there is no need for sample labeling, fixation or any other specific preparation. In addition, we demonstrate that the ICA is suitable for THG images, although it is primarily developed and have been mostly used for fluorescence signals so far. REFERENCES [1] D. Débarre, W. Supatto et al., Nat. Methods, 3(1), (2006), 47-53. [2] R. W. Boyd, Nonlinear Optics, Academic Press (2008).",
publisher = "Belgrade: Institute of Physics",
journal = "Book of Abstracts: 15th Photonics Workshop: Conference; 2022 Mar 13-16; Kopaonik, Serbia",
title = "Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi",
pages = "52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5735"
}

Pajić, T., Todorović, N., Živić, M., Rabasović, M.,& Krmpot, A.. (2022). Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi. in Book of Abstracts: 15th Photonics Workshop: Conference; 2022 Mar 13-16; Kopaonik, Serbia
Belgrade: Institute of Physics., 52.
https://hdl.handle.net/21.15107/rcub_ibiss_5735

Pajić T, Todorović N, Živić M, Rabasović M, Krmpot A. Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi. in Book of Abstracts: 15th Photonics Workshop: Conference; 2022 Mar 13-16; Kopaonik, Serbia. 2022;:52.
https://hdl.handle.net/21.15107/rcub_ibiss_5735 .

Pajić, Tanja, Todorović, Nataša, Živić, Miroslav, Rabasović, Mihailo, Krmpot, Aleksandar, "Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi" in Book of Abstracts: 15th Photonics Workshop: Conference; 2022 Mar 13-16; Kopaonik, Serbia (2022):52,
https://hdl.handle.net/21.15107/rcub_ibiss_5735 .

TY  - CONF
AU  - Pajić, Tanja
AU  - Živić, Miroslav
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar J.
AU  - Todorović, Nataša
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4722
AB  - Lipid droplets (LDs) are small mobile organelles conserved in all eukaryotic cells. We wanted to 
test if the LD movement can be muffled by an incomplete inhibition of mitochondrial respiration, 
induced by treating hyphae of filamentous fungus Phycomyces blakesleeanus with 0.5 mM sodium 
azide. Nitrogen starved hyphae were used, in order to obtain LDs in larger sizes and numbers. The data 
obtained unequivocally showed: 1. Sodium azide treatment dramatically reduces the LD velocity and 
the distances LDs travel; 2. LDs in both controls and in azide-treated hyphae oscillate in a small 
confined space instead of travelling through the cell; 3. Azide-treated LDs oscillate less frequently and 
in smaller confinement than controls.
PB  - Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac
C3  - 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021
T1  - The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor
DO  - 10.46793/ICCBI21.226P
SP  - 226
EP  - 229
ER  - 

@conference{
author = "Pajić, Tanja and Živić, Miroslav and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Todorović, Nataša",
year = "2021",
abstract = "Lipid droplets (LDs) are small mobile organelles conserved in all eukaryotic cells. We wanted to 
test if the LD movement can be muffled by an incomplete inhibition of mitochondrial respiration, 
induced by treating hyphae of filamentous fungus Phycomyces blakesleeanus with 0.5 mM sodium 
azide. Nitrogen starved hyphae were used, in order to obtain LDs in larger sizes and numbers. The data 
obtained unequivocally showed: 1. Sodium azide treatment dramatically reduces the LD velocity and 
the distances LDs travel; 2. LDs in both controls and in azide-treated hyphae oscillate in a small 
confined space instead of travelling through the cell; 3. Azide-treated LDs oscillate less frequently and 
in smaller confinement than controls.",
publisher = "Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac",
journal = "1st International Conference on Chemo and BioInformatics, ICCBIKG 2021",
title = "The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor",
doi = "10.46793/ICCBI21.226P",
pages = "226-229"
}

Pajić, T., Živić, M., Rabasović, M. D., Krmpot, A. J.,& Todorović, N.. (2021). The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021
Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac., 226-229.
https://doi.org/10.46793/ICCBI21.226P

Pajić T, Živić M, Rabasović MD, Krmpot AJ, Todorović N. The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021. 2021;:226-229.
doi:10.46793/ICCBI21.226P .

Pajić, Tanja, Živić, Miroslav, Rabasović, Mihailo D., Krmpot, Aleksandar J., Todorović, Nataša, "The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor" in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021 (2021):226-229,
https://doi.org/10.46793/ICCBI21.226P . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Todorović, Nataša
AU  - Stefanović, Dunja
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar J.
AU  - Živić, Miroslav
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4721
AB  - Selenium salts have been known for long time to have a potential for both beneficial and harmful 
effects on living organisms. It is present in the environment, where it can be readily assimilated by 
plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence 
of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 
mM) on the growth of fungi from the activated spores to the end of the exponential growth were 
measured on several hypha morphological parameters by microscopy in vivo. Phycomyces 
blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was 
inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group 
than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential 
growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in 
growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4
-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not 
significantly altered.
PB  - Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac
C3  - 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021
T1  - The influence of selenite on fillamentous fungi hypha morphometry parameters
DO  - 10.46793/ICCBI21.308P
SP  - 308
EP  - 311
ER  - 

@conference{
author = "Pajić, Tanja and Todorović, Nataša and Stefanović, Dunja and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Živić, Miroslav",
year = "2021",
abstract = "Selenium salts have been known for long time to have a potential for both beneficial and harmful 
effects on living organisms. It is present in the environment, where it can be readily assimilated by 
plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence 
of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 
mM) on the growth of fungi from the activated spores to the end of the exponential growth were 
measured on several hypha morphological parameters by microscopy in vivo. Phycomyces 
blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was 
inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group 
than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential 
growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in 
growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4
-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not 
significantly altered.",
publisher = "Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac",
journal = "1st International Conference on Chemo and BioInformatics, ICCBIKG 2021",
title = "The influence of selenite on fillamentous fungi hypha morphometry parameters",
doi = "10.46793/ICCBI21.308P",
pages = "308-311"
}

Pajić, T., Todorović, N., Stefanović, D., Rabasović, M. D., Krmpot, A. J.,& Živić, M.. (2021). The influence of selenite on fillamentous fungi hypha morphometry parameters. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021
Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac., 308-311.
https://doi.org/10.46793/ICCBI21.308P

Pajić T, Todorović N, Stefanović D, Rabasović MD, Krmpot AJ, Živić M. The influence of selenite on fillamentous fungi hypha morphometry parameters. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021. 2021;:308-311.
doi:10.46793/ICCBI21.308P .

Pajić, Tanja, Todorović, Nataša, Stefanović, Dunja, Rabasović, Mihailo D., Krmpot, Aleksandar J., Živić, Miroslav, "The influence of selenite on fillamentous fungi hypha morphometry parameters" in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021 (2021):308-311,
https://doi.org/10.46793/ICCBI21.308P . .

TY  - CONF
AU  - Pajić, Tanja
AU  - Todorović, Nataša
AU  - Stefanović, Dunja
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar J.
AU  - Živić, Miroslav
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4680
AB  - Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non-uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 - 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4-treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.
PB  - Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac
C3  - 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
T1  - The effects of selenite on filamentous fungi lipid droplets monitored in vivo label free using advanced nonlinear microscopy technique
DO  - 10.46793/ICCBI21.300P
SP  - 300
EP  - 303
ER  - 

@conference{
author = "Pajić, Tanja and Todorović, Nataša and Stefanović, Dunja and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Živić, Miroslav",
year = "2021",
abstract = "Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non-uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 - 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4-treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.",
publisher = "Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac",
journal = "1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia",
title = "The effects of selenite on filamentous fungi lipid droplets monitored in vivo label free using advanced nonlinear microscopy technique",
doi = "10.46793/ICCBI21.300P",
pages = "300-303"
}

Pajić, T., Todorović, N., Stefanović, D., Rabasović, M. D., Krmpot, A. J.,& Živić, M.. (2021). The effects of selenite on filamentous fungi lipid droplets monitored in vivo label free using advanced nonlinear microscopy technique. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac., 300-303.
https://doi.org/10.46793/ICCBI21.300P

Pajić T, Todorović N, Stefanović D, Rabasović MD, Krmpot AJ, Živić M. The effects of selenite on filamentous fungi lipid droplets monitored in vivo label free using advanced nonlinear microscopy technique. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia. 2021;:300-303.
doi:10.46793/ICCBI21.300P .

Pajić, Tanja, Todorović, Nataša, Stefanović, Dunja, Rabasović, Mihailo D., Krmpot, Aleksandar J., Živić, Miroslav, "The effects of selenite on filamentous fungi lipid droplets monitored in vivo label free using advanced nonlinear microscopy technique" in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia (2021):300-303,
https://doi.org/10.46793/ICCBI21.300P . .

TY  - CONF
AU  - Todorović, Nataša
AU  - Stojadinović, Gordana
AU  - AlJamal, Kamal
AU  - Živić, Miroslav
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4678
AB  - Unlike the neurons in the CNS, the peripheral neurons have certain intrinsic regenerative capacity. 
After injury, peripheral neurons can switch to a cellular “state for growth”, with the expression profiles 
similar to early developmental stages. 
We looked at the changes of morphometric parameters induced in young peripheral neurons with 
treatments that in adult neurons have growth-stimulatory effect. The experimental treatments compared 
to control were: BpV (phen), an inhibitor of PTEN; and bFGF, basic fibroblast growth factor. The 
neurite growth was measured on cultured dissociated dorsal root ganglia neonatal neurons fixed 24h 
after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI 
Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, compared 
to control, total neurite length, length of primary and length of terminal branches, were increased by 
bFGF but not by BpV treatment. In all measured parameters related to the degree of branching, BpV treated neurons had small dispersion of values and small mean values, reminiscent of literature data 
stating that BpV treated neurons are elongated and less branched. However, the BpV did not have a 
positive influence on neurite elongation, as was reported on adult neurons. In contrast, bFGF stimulated 
elongation of young neurons in the manner similar to the effects described on the adult neurons.
PB  - Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac
C3  - 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
T1  - The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture
DO  - 10.46793/ICCBI21.214T
SP  - 214
EP  - 217
ER  - 

@conference{
author = "Todorović, Nataša and Stojadinović, Gordana and AlJamal, Kamal and Živić, Miroslav",
year = "2021",
abstract = "Unlike the neurons in the CNS, the peripheral neurons have certain intrinsic regenerative capacity. 
After injury, peripheral neurons can switch to a cellular “state for growth”, with the expression profiles 
similar to early developmental stages. 
We looked at the changes of morphometric parameters induced in young peripheral neurons with 
treatments that in adult neurons have growth-stimulatory effect. The experimental treatments compared 
to control were: BpV (phen), an inhibitor of PTEN; and bFGF, basic fibroblast growth factor. The 
neurite growth was measured on cultured dissociated dorsal root ganglia neonatal neurons fixed 24h 
after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI 
Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, compared 
to control, total neurite length, length of primary and length of terminal branches, were increased by 
bFGF but not by BpV treatment. In all measured parameters related to the degree of branching, BpV treated neurons had small dispersion of values and small mean values, reminiscent of literature data 
stating that BpV treated neurons are elongated and less branched. However, the BpV did not have a 
positive influence on neurite elongation, as was reported on adult neurons. In contrast, bFGF stimulated 
elongation of young neurons in the manner similar to the effects described on the adult neurons.",
publisher = "Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac",
journal = "1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia",
title = "The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture",
doi = "10.46793/ICCBI21.214T",
pages = "214-217"
}

Todorović, N., Stojadinović, G., AlJamal, K.,& Živić, M.. (2021). The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia
Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac., 214-217.
https://doi.org/10.46793/ICCBI21.214T

Todorović N, Stojadinović G, AlJamal K, Živić M. The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture. in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia. 2021;:214-217.
doi:10.46793/ICCBI21.214T .

Todorović, Nataša, Stojadinović, Gordana, AlJamal, Kamal, Živić, Miroslav, "The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture" in 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia (2021):214-217,
https://doi.org/10.46793/ICCBI21.214T . .

TY  - JOUR
AU  - Stanić, Marin
AU  - Križak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajić, Tanja
AU  - Ćirić, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antić, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
PY  - 2017
UR  - http://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000429
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2730
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 μM NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33±5 and 21±3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
T2  - Microbiology
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
IS  - 3
VL  - 163
DO  - 10.1099/mic.0.000429
SP  - 364
EP  - 372
ER  - 

@article{
author = "Stanić, Marin and Križak, Strahinja and Jovanović, Mirna and Pajić, Tanja and Ćirić, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antić, Tijana and Todorović, Nataša and Živić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 μM NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33±5 and 21±3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
journal = "Microbiology",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429",
pages = "364-372"
}

Stanić, M., Križak, S., Jovanović, M., Pajić, T., Ćirić, A., Žižić, M., Zakrzewska, J., Cvetić Antić, T., Todorović, N.,& Živić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology, 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Križak S, Jovanović M, Pajić T, Ćirić A, Žižić M, Zakrzewska J, Cvetić Antić T, Todorović N, Živić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marin, Križak, Strahinja, Jovanović, Mirna, Pajić, Tanja, Ćirić, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antić, Tijana, Todorović, Nataša, Živić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .