TY  - CONF
AU  - Ognjanović, Branka
AU  - Pavlović, Slađan
AU  - Maletić, Snežana
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
PY  - 2003
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5963
AB  - Cadmium (Cd) is an ubiquitous toxic metal that is involved in a variety of pathological condicions. The present study investigated the po-,sible protective role of vitamin E (Vit E) in cadmium induced oxidative stress in the blood of rats. Male, Wistar albino rats 3 months old (weighing 280 ± 30 g) were used in our experiments. Rats were injected with a single dose (1) of CdClz (0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing), (2)Vit E + Cd (20 UI Vit E/kg b.m., i.m., 48h +0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing). The group of animals without treatment was control. The activities of enzymatic components of AOS: copper zinc containing superoxide-dismutase (CuZn SOD), catalase (CAT), glutathione-peroxidase   (GSH­
Px), glutathione-reductase (GR) and glutathione-S-transferase (GST) were  analyzed
in erythrocytes  and concentrations of non-enzymatic  components of  AOS: reduced	'
glutathione   (GSH),  ascorbic  acid  (AsA)  and  vitamin   E  (Vit  E),  as  well  as  lipid    peroxide  (LP) concentration  were determined  in the blood of rats.  Acute intoxication	I of rats with  Cd  were followed  by significantly  increased  LP  and increased activities		I
of enzymatic, as well as concentrations of non-enzymatic components of AOS in blood. The pretreatment with vitamin E after acute intoxication of rats with Cd partialy improved components of AOS. Our results indicate that liposoluble antioxidant (Vit E) resulted as suitable and potent antioxidant action, probably as consequence of quenchening of reactive oxygen species, inhibiton of lipid peroxidation and prevention of free-radical mediated injuries. The results of this study suggested that vitamin E functions as antioxygenic nutrients.
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia
T1  - Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5963
ER  - 

@conference{
author = "Ognjanović, Branka and Pavlović, Slađan and Maletić, Snežana and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica",
year = "2003",
abstract = "Cadmium (Cd) is an ubiquitous toxic metal that is involved in a variety of pathological condicions. The present study investigated the po-,sible protective role of vitamin E (Vit E) in cadmium induced oxidative stress in the blood of rats. Male, Wistar albino rats 3 months old (weighing 280 ± 30 g) were used in our experiments. Rats were injected with a single dose (1) of CdClz (0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing), (2)Vit E + Cd (20 UI Vit E/kg b.m., i.m., 48h +0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing). The group of animals without treatment was control. The activities of enzymatic components of AOS: copper zinc containing superoxide-dismutase (CuZn SOD), catalase (CAT), glutathione-peroxidase   (GSH­
Px), glutathione-reductase (GR) and glutathione-S-transferase (GST) were  analyzed
in erythrocytes  and concentrations of non-enzymatic  components of  AOS: reduced	'
glutathione   (GSH),  ascorbic  acid  (AsA)  and  vitamin   E  (Vit  E),  as  well  as  lipid    peroxide  (LP) concentration  were determined  in the blood of rats.  Acute intoxication	I of rats with  Cd  were followed  by significantly  increased  LP  and increased activities		I
of enzymatic, as well as concentrations of non-enzymatic components of AOS in blood. The pretreatment with vitamin E after acute intoxication of rats with Cd partialy improved components of AOS. Our results indicate that liposoluble antioxidant (Vit E) resulted as suitable and potent antioxidant action, probably as consequence of quenchening of reactive oxygen species, inhibiton of lipid peroxidation and prevention of free-radical mediated injuries. The results of this study suggested that vitamin E functions as antioxygenic nutrients.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia",
title = "Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5963"
}

Ognjanović, B., Pavlović, S., Maletić, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z.. (2003). Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E. in Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia
Belgrade: Serbian Physiology Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5963

Ognjanović B, Pavlović S, Maletić S, Žikić RV, Štajn AŠ, Saičić Z. Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E. in Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia. 2003;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5963 .

Ognjanović, Branka, Pavlović, Slađan, Maletić, Snežana, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica, "Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E" in Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia (2003):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5963 .

TY  - CONF
AU  - Pavlović, Slađan
AU  - Maletić, Snežana
AU  - Ognjanović, Branka
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
AU  - Petrović, Vojislav M.
PY  - 2001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5968
AB  - In our experiments male 2-month-old Wistar albino rats were divided as follows: (i) untreated controls (C); (ii) animals treated with 14 mg/day/kg· b.m. cadmium in drinking water during 15 days (Cd); (iii) rats treated with 20 I/kg/dose of Vitamin E (Vit E) and 15 m/kg/dose of coenzyme Q10 (CoQ10) i.m., every fifth day during 15 days (Vit E + CoQIO) and (iv) animals pretreated with Vit E and CoQ10 in
above mentioned amounts during 15 days, and then treated with 14 m/day/kg b.m. of Cd during next 15 days (Cd + Vit E + CoQ10). We measured the following parameters of energy metabolism: glucose (Gly), lactate (Lac) and pyruvate (Pyr) levels , Lac/Pyr ratio, ATP, ADP and AMP levels, ATP/ADP ratio, total adenine nucleotide (TAN) levels and adenylate energy charge (AEC). All obtained results were compared in respect to the control animals. Our results show that Gly level did not changed, while Lac and Pyr levels were significantly increased in Vit E + CoQ10 treated rats. At the same time Lac/Pyr ratio was significantly decreased. In Cd and Cd + Vit E + CoQ10 treated animals ATP level was significantly decreased, whereas AMP level was significantly increased. ADP  level  did  not  significantly  alter  in  any  of  examined  group  of  animals. A significant decrease of ATP/ADP ratio and TAN levels in the same experimental groups were observed. AEC did not alter in any of investigated group of rats. It can be concluded that Vit E and CoQ IO have partial protect i, ·e role on energy metabolism in the blood of rats influenced by Cd.
PB  - Bleckwell Science
C3  - 27th Meeting of the Federation of European Biochemical Societies; 2001 Jun 30 - Jul 5; Lisbon, Portugal
T1  - The influence of cadmium on energy metabolism in the blood of rats. A possible protective role of vitamin E and coenzyme Q10
SP  - 238
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5968
ER  - 

@conference{
author = "Pavlović, Slađan and Maletić, Snežana and Ognjanović, Branka and Štajn, Andraš Š. and Žikić, Radoslav V. and Saičić, Zorica and Petrović, Vojislav M.",
year = "2001",
abstract = "In our experiments male 2-month-old Wistar albino rats were divided as follows: (i) untreated controls (C); (ii) animals treated with 14 mg/day/kg· b.m. cadmium in drinking water during 15 days (Cd); (iii) rats treated with 20 I/kg/dose of Vitamin E (Vit E) and 15 m/kg/dose of coenzyme Q10 (CoQ10) i.m., every fifth day during 15 days (Vit E + CoQIO) and (iv) animals pretreated with Vit E and CoQ10 in
above mentioned amounts during 15 days, and then treated with 14 m/day/kg b.m. of Cd during next 15 days (Cd + Vit E + CoQ10). We measured the following parameters of energy metabolism: glucose (Gly), lactate (Lac) and pyruvate (Pyr) levels , Lac/Pyr ratio, ATP, ADP and AMP levels, ATP/ADP ratio, total adenine nucleotide (TAN) levels and adenylate energy charge (AEC). All obtained results were compared in respect to the control animals. Our results show that Gly level did not changed, while Lac and Pyr levels were significantly increased in Vit E + CoQ10 treated rats. At the same time Lac/Pyr ratio was significantly decreased. In Cd and Cd + Vit E + CoQ10 treated animals ATP level was significantly decreased, whereas AMP level was significantly increased. ADP  level  did  not  significantly  alter  in  any  of  examined  group  of  animals. A significant decrease of ATP/ADP ratio and TAN levels in the same experimental groups were observed. AEC did not alter in any of investigated group of rats. It can be concluded that Vit E and CoQ IO have partial protect i, ·e role on energy metabolism in the blood of rats influenced by Cd.",
publisher = "Bleckwell Science",
journal = "27th Meeting of the Federation of European Biochemical Societies; 2001 Jun 30 - Jul 5; Lisbon, Portugal",
title = "The influence of cadmium on energy metabolism in the blood of rats. A possible protective role of vitamin E and coenzyme Q10",
pages = "238",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5968"
}

Pavlović, S., Maletić, S., Ognjanović, B., Štajn, A. Š., Žikić, R. V., Saičić, Z.,& Petrović, V. M.. (2001). The influence of cadmium on energy metabolism in the blood of rats. A possible protective role of vitamin E and coenzyme Q10. in 27th Meeting of the Federation of European Biochemical Societies; 2001 Jun 30 - Jul 5; Lisbon, Portugal
Bleckwell Science., 238.
https://hdl.handle.net/21.15107/rcub_ibiss_5968

Pavlović S, Maletić S, Ognjanović B, Štajn AŠ, Žikić RV, Saičić Z, Petrović VM. The influence of cadmium on energy metabolism in the blood of rats. A possible protective role of vitamin E and coenzyme Q10. in 27th Meeting of the Federation of European Biochemical Societies; 2001 Jun 30 - Jul 5; Lisbon, Portugal. 2001;:238.
https://hdl.handle.net/21.15107/rcub_ibiss_5968 .

Pavlović, Slađan, Maletić, Snežana, Ognjanović, Branka, Štajn, Andraš Š., Žikić, Radoslav V., Saičić, Zorica, Petrović, Vojislav M., "The influence of cadmium on energy metabolism in the blood of rats. A possible protective role of vitamin E and coenzyme Q10" in 27th Meeting of the Federation of European Biochemical Societies; 2001 Jun 30 - Jul 5; Lisbon, Portugal (2001):238,
https://hdl.handle.net/21.15107/rcub_ibiss_5968 .

TY  - CONF
AU  - Pavlović, Slađan
AU  - Ognjanović, Branka
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
AU  - Petrović, Vojislav M.
PY  - 2000
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6031
AB  - The aim of our study was to investigate the activities of total superoxide dismutase (Total SOD), manganese containing superoxide dismutase (Mn SOD), copper zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR) as well as ascorbic acid (AsA) and vitamin E (Vit E) concentrations in the heart of male, two months old Wistar albino rats. The animals were divided in four experimental groups: (1) controls (C), (2) treated with cadmium (Cd, 200 mg CdCl2 x H2O, in drinking water during 30 days), (3) treated with coenzyme Q10 (CoQ10, 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day) and (4) concomitantly treated with cadmium and coenzyme Q10 (Cd+CoQ10, 200 mg CdCl2 x H2O, in drinking water during 30 days + 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day). An average intake of           17 mg Cd/day/kg body mass was calculated from the water consumed and an average intake of CoQ10 was 16 mg/kg/dose. Each experimental group consisted of seven animals. After the treatment the rats were sacrificed by decapitation and heart tissue was isolated and prepared for analysis.
	The obtained results show that Cd in the heart induces a significant increase of Total SOD, CuZn SOD and GSH-Px activities as well as AsA and Vit E concentrations in comparison to the control rats. At the same time, treatment with Cd leads to a significant decrease of CAT and GR activities. In the heart of rats treated with CoQ10 a significant increase of Total SOD, Mn SOD, CuZn SOD and GSH-Px activities as well as AsA and Vit E concentrations were observed. In the same group of animals, the activities of CAT, GST and GR were significantly decreased in respect to the control rats. By concomitant treatment of rats with Cd+CoQ10 the activities of Total SOD and Mn SOD were markedly increased in relation to the controls, while CuZn SOD activity was significantly decreased. In the animals concomitantly treated with Cd+CoQ10 the activities of CAT and GR were significantly decreased, whereas     GSH-Px activity and AsA and Vit E concentrations were significantly increased in relation to the control rats.
	From the presented results, it may be concluded that Cd and CoQ10 in the dosages administered influenced antioxidant defence system (AOS) in the heart of rats. The protective role of CoQ10 on AOS in the heart of rats against Cd toxicity was also observed.
PB  - Society of free radical research, Europe
C3  - Abstract Book: 2nd International Meeting on Oxidative Stress: Biochemistry and Pathophysiology: Winter Meeting of Society of Free radical Research - Europe; 2000 Nov 30 - Dec 2; Valencia, Spain
T1  - Changes of antioxidant defence system in the heart of rats treated with cadmium and coenzyme Q10
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6031
ER  - 

@conference{
author = "Pavlović, Slađan and Ognjanović, Branka and Štajn, Andraš Š. and Žikić, Radoslav V. and Saičić, Zorica and Petrović, Vojislav M.",
year = "2000",
abstract = "The aim of our study was to investigate the activities of total superoxide dismutase (Total SOD), manganese containing superoxide dismutase (Mn SOD), copper zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR) as well as ascorbic acid (AsA) and vitamin E (Vit E) concentrations in the heart of male, two months old Wistar albino rats. The animals were divided in four experimental groups: (1) controls (C), (2) treated with cadmium (Cd, 200 mg CdCl2 x H2O, in drinking water during 30 days), (3) treated with coenzyme Q10 (CoQ10, 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day) and (4) concomitantly treated with cadmium and coenzyme Q10 (Cd+CoQ10, 200 mg CdCl2 x H2O, in drinking water during 30 days + 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day). An average intake of           17 mg Cd/day/kg body mass was calculated from the water consumed and an average intake of CoQ10 was 16 mg/kg/dose. Each experimental group consisted of seven animals. After the treatment the rats were sacrificed by decapitation and heart tissue was isolated and prepared for analysis.
	The obtained results show that Cd in the heart induces a significant increase of Total SOD, CuZn SOD and GSH-Px activities as well as AsA and Vit E concentrations in comparison to the control rats. At the same time, treatment with Cd leads to a significant decrease of CAT and GR activities. In the heart of rats treated with CoQ10 a significant increase of Total SOD, Mn SOD, CuZn SOD and GSH-Px activities as well as AsA and Vit E concentrations were observed. In the same group of animals, the activities of CAT, GST and GR were significantly decreased in respect to the control rats. By concomitant treatment of rats with Cd+CoQ10 the activities of Total SOD and Mn SOD were markedly increased in relation to the controls, while CuZn SOD activity was significantly decreased. In the animals concomitantly treated with Cd+CoQ10 the activities of CAT and GR were significantly decreased, whereas     GSH-Px activity and AsA and Vit E concentrations were significantly increased in relation to the control rats.
	From the presented results, it may be concluded that Cd and CoQ10 in the dosages administered influenced antioxidant defence system (AOS) in the heart of rats. The protective role of CoQ10 on AOS in the heart of rats against Cd toxicity was also observed.",
publisher = "Society of free radical research, Europe",
journal = "Abstract Book: 2nd International Meeting on Oxidative Stress: Biochemistry and Pathophysiology: Winter Meeting of Society of Free radical Research - Europe; 2000 Nov 30 - Dec 2; Valencia, Spain",
title = "Changes of antioxidant defence system in the heart of rats treated with cadmium and coenzyme Q10",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6031"
}

Pavlović, S., Ognjanović, B., Štajn, A. Š., Žikić, R. V., Saičić, Z.,& Petrović, V. M.. (2000). Changes of antioxidant defence system in the heart of rats treated with cadmium and coenzyme Q10. in Abstract Book: 2nd International Meeting on Oxidative Stress: Biochemistry and Pathophysiology: Winter Meeting of Society of Free radical Research - Europe; 2000 Nov 30 - Dec 2; Valencia, Spain
Society of free radical research, Europe., 81-81.
https://hdl.handle.net/21.15107/rcub_ibiss_6031

Pavlović S, Ognjanović B, Štajn AŠ, Žikić RV, Saičić Z, Petrović VM. Changes of antioxidant defence system in the heart of rats treated with cadmium and coenzyme Q10. in Abstract Book: 2nd International Meeting on Oxidative Stress: Biochemistry and Pathophysiology: Winter Meeting of Society of Free radical Research - Europe; 2000 Nov 30 - Dec 2; Valencia, Spain. 2000;:81-81.
https://hdl.handle.net/21.15107/rcub_ibiss_6031 .

Pavlović, Slađan, Ognjanović, Branka, Štajn, Andraš Š., Žikić, Radoslav V., Saičić, Zorica, Petrović, Vojislav M., "Changes of antioxidant defence system in the heart of rats treated with cadmium and coenzyme Q10" in Abstract Book: 2nd International Meeting on Oxidative Stress: Biochemistry and Pathophysiology: Winter Meeting of Society of Free radical Research - Europe; 2000 Nov 30 - Dec 2; Valencia, Spain (2000):81-81,
https://hdl.handle.net/21.15107/rcub_ibiss_6031 .