TY  - CONF
AU  - Milošević, Verica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Marković, Z
AU  - Manojlović-Stojanoski, Milica
AU  - Jarić, Ivana
AU  - Brkić, Branislava
AU  - Sekulić, Milka I.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1293
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol
IS  - null
VL  - 49
EP  - S435
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1293
ER  - 

@conference{
author = "Milošević, Verica and Trifunović, Svetlana and Ajdžanović, Vladimir and Marković, Z and Manojlović-Stojanoski, Milica and Jarić, Ivana and Brkić, Branislava and Sekulić, Milka I.",
year = "2011",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol",
number = "null",
volume = "49",
pages = "S435",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1293"
}

Milošević, V., Trifunović, S., Ajdžanović, V., Marković, Z., Manojlović-Stojanoski, M., Jarić, I., Brkić, B.,& Sekulić, M. I.. (2011). Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol. in Clinical Chemistry and Laboratory Medicine, 49(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1293

Milošević V, Trifunović S, Ajdžanović V, Marković Z, Manojlović-Stojanoski M, Jarić I, Brkić B, Sekulić MI. Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol. in Clinical Chemistry and Laboratory Medicine. 2011;49(null):null-S435.
https://hdl.handle.net/21.15107/rcub_ibiss_1293 .

Milošević, Verica, Trifunović, Svetlana, Ajdžanović, Vladimir, Marković, Z, Manojlović-Stojanoski, Milica, Jarić, Ivana, Brkić, Branislava, Sekulić, Milka I., "Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol" in Clinical Chemistry and Laboratory Medicine, 49, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1293 .

TY  - CONF
AU  - Brkić, Branislava
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Đelić, Marina N
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1292
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Adiposity Effects of Centrally Administered Ghrelin in Aged Rats
IS  - null
VL  - 49
EP  - S631
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1292
ER  - 

@conference{
author = "Brkić, Branislava and Nesić, Dejan M and Stevanović, Darko M and Đelić, Marina N and Milošević, Verica and Starčević, Vesna P.",
year = "2011",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats",
number = "null",
volume = "49",
pages = "S631",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1292"
}

Brkić, B., Nesić, D. M., Stevanović, D. M., Đelić, M. N., Milošević, V.,& Starčević, V. P.. (2011). Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine, 49(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1292

Brkić B, Nesić DM, Stevanović DM, Đelić MN, Milošević V, Starčević VP. Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine. 2011;49(null):null-S631.
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .

Brkić, Branislava, Nesić, Dejan M, Stevanović, Darko M, Đelić, Marina N, Milošević, Verica, Starčević, Vesna P., "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats" in Clinical Chemistry and Laboratory Medicine, 49, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .

TY  - JOUR
AU  - Kostić, Nada
AU  - Brkić, Branislava
AU  - Čaparević, Zorica
AU  - Milošević, Verica
PY  - 2004
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/36
AB  - Serum levels of somatostatin were determined by RIA method in 50 patients with liver cirrhosis, 15 with pernicious anemia and atrophic gastritis, 31 with inflamatory bowel disease, 32 with colorectal tumors and in 40 control persons. In patients with liver cirrhosis somatostatin levels were significantly higher than in control group (p<0.01). Patients with pernicious anemia and ulcerative colitis in acute phase of the disease had significantly lower levels of somatostatin (p<0.01) (p<0.005). In patients with colorectal tumors somatostatin were significantly lower then in control group (p<0.01). Our results show that the liver is involved in somatostatin metabolism. Atrophic gastritis, ulcerative colitis and M. Crohn, so as colorectal neoplasia were associated with significantly changes in somatostatin levels which suggest the potential pathophysiologic and terapeutic role of somatostatin in those disease.
AB  - Somatostatin u serumu određen je RIA metodom, kod 50 pacijenata sa cirozom jetre, 15 sa pernicioznom anemijom i atrofičnim gastritisom, 31 sa inflamatornim oboljenjem creva, 32 sa kolorektalnim tumorima i 40 kontrolnih osoba. Kod pacijenata sa cirozom jetre nađene su značajno više vrednosti somatostatina u odnosu na kontrolnu grupu (p<0,01). Pacijenti sa pernicioznom anemijom i ulceroznim kolitisom u akutnoj fazi bolesti imali su značajno niže vrednosti somatostatina (p<0,005). Kod pacijenta sa kolorektalnim tumorima somatostatin je bio značajno niži nego u kontrolnoj grupi (p<0,01). Dobijeni rezultati pokazuju da je jetra uključena u metabolizam somatostatina. Atrofični gastritis, ulcerozni kolitis i M. Crohn, kao i kolorektalni tumori bili su udruženi sa značajnim promenama u nivou somatostatina što sugeriše moguću patofiziološku i terapeutsku ulogu somatostatina u ovim oboljenjima.
T2  - Jugoslovenska medicinska biohemija
T1  - Somatostatin u oboljenjima gastrointestinalnog trakta
T1  - Somatostatin levels in gastrointestinal disease
IS  - 4
VL  - 23
SP  - 393
EP  - 396
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_36
ER  - 

@article{
author = "Kostić, Nada and Brkić, Branislava and Čaparević, Zorica and Milošević, Verica",
year = "2004, 2004",
abstract = "Serum levels of somatostatin were determined by RIA method in 50 patients with liver cirrhosis, 15 with pernicious anemia and atrophic gastritis, 31 with inflamatory bowel disease, 32 with colorectal tumors and in 40 control persons. In patients with liver cirrhosis somatostatin levels were significantly higher than in control group (p<0.01). Patients with pernicious anemia and ulcerative colitis in acute phase of the disease had significantly lower levels of somatostatin (p<0.01) (p<0.005). In patients with colorectal tumors somatostatin were significantly lower then in control group (p<0.01). Our results show that the liver is involved in somatostatin metabolism. Atrophic gastritis, ulcerative colitis and M. Crohn, so as colorectal neoplasia were associated with significantly changes in somatostatin levels which suggest the potential pathophysiologic and terapeutic role of somatostatin in those disease., Somatostatin u serumu određen je RIA metodom, kod 50 pacijenata sa cirozom jetre, 15 sa pernicioznom anemijom i atrofičnim gastritisom, 31 sa inflamatornim oboljenjem creva, 32 sa kolorektalnim tumorima i 40 kontrolnih osoba. Kod pacijenata sa cirozom jetre nađene su značajno više vrednosti somatostatina u odnosu na kontrolnu grupu (p<0,01). Pacijenti sa pernicioznom anemijom i ulceroznim kolitisom u akutnoj fazi bolesti imali su značajno niže vrednosti somatostatina (p<0,005). Kod pacijenta sa kolorektalnim tumorima somatostatin je bio značajno niži nego u kontrolnoj grupi (p<0,01). Dobijeni rezultati pokazuju da je jetra uključena u metabolizam somatostatina. Atrofični gastritis, ulcerozni kolitis i M. Crohn, kao i kolorektalni tumori bili su udruženi sa značajnim promenama u nivou somatostatina što sugeriše moguću patofiziološku i terapeutsku ulogu somatostatina u ovim oboljenjima.",
journal = "Jugoslovenska medicinska biohemija",
title = "Somatostatin u oboljenjima gastrointestinalnog trakta, Somatostatin levels in gastrointestinal disease",
number = "4",
volume = "23",
pages = "393-396",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_36"
}

Kostić, N., Brkić, B., Čaparević, Z.,& Milošević, V.. (2004). Somatostatin u oboljenjima gastrointestinalnog trakta. in Jugoslovenska medicinska biohemija, 23(4), 393-396.
https://hdl.handle.net/21.15107/rcub_ibiss_36

Kostić N, Brkić B, Čaparević Z, Milošević V. Somatostatin u oboljenjima gastrointestinalnog trakta. in Jugoslovenska medicinska biohemija. 2004;23(4):393-396.
https://hdl.handle.net/21.15107/rcub_ibiss_36 .

Kostić, Nada, Brkić, Branislava, Čaparević, Zorica, Milošević, Verica, "Somatostatin u oboljenjima gastrointestinalnog trakta" in Jugoslovenska medicinska biohemija, 23, no. 4 (2004):393-396,
https://hdl.handle.net/21.15107/rcub_ibiss_36 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Manojlović-Stojanoski, Milica
AU  - Kostić, Nada
AU  - Brkić, Branislava
AU  - Velkovski, Saško D.
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Starčević, Vesna P.
PY  - 2003
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/67
AB  - The results on the effects of synthetic somatostatins on the secretion of ACTH have been rather conflicting, but some authors reported that somatostain is involved in the regulation of ACTH secretion in rats of both sexes. The inhibitory mechanism of ACTH secretion could operate through the inhibition of CRF release from the hypothalamus. In the present study, we have investigated the effects of Octreotide, a somatostain analogue given intracerebroventricularly (i.c.v) in low doses, on morphometric characteristics of pituitary adrenocorticotropes (ACTH cells) in adult Wistar rat males. The animals were i.c.v. given three 1.0 µg doses of Octreotide dissolved in 10 µL saline every second day. The controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Body weight, as well as absolute and relative pituitary weights were not significantly changed in comparison with the corresponding controls (p>0.05). The characteristics of immunohistochemicaly labelled ACTH cells in intact rat males were as follows: localization between the capillaries stellate in shape with the cytoplasmatic processes among neighbouring cells. The nucleus follows the shape of the cell body. Small specific secretory granules were distributed mainly at the periphery of the cytoplasm. The shape, and localization of ACTH immunoreactive cells in Octreotide-treated animals were not significantly changed in comparison with the controls. Octreotide treatment significantly decreased all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 44% and 18% respectively, as compared to the controls (p<0.05). Volume densities were also significantly decreased (by 31%; p<0.05) comparing to the corresponding controls. These results indicate that Octreotide applied i.c.v. exerts a significant inhibitory effect on the immunohistochemical and morphometric characteristics of ACTH cells in rat males.
AB  - Postoje veoma različita, a često i suprotstavljena mišljenja o efektima sintetskih somatostatina na lučenje adrenokortikotropnog hormona (ACTH) iz hipofize. Mogući mehanizam inhibicije lučenja ACTH je preko inhibicije lučenja CRF iz hipotalamusa. U ovom radu su ispitivani efekti intracerebroventrikularno (i.c.v) ubrizganog analoga somatostatina Oktreotida, na rast ACTH ćelija adenohipofize mužjaka Wistar pacova. Sve eksperimentalne životinje su primile tri doze od po 1.0 µg Oktreotida rastvorenog u 10 µL fiziološkog rastvora, svakog drugog dana. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani pet dana posle poslednje injekcije. ACTH ćelije su imunocitohemijski bojene metodom peroksidaza-antiperoksidaza (PAP). Telesna masa, apsolutna i relativna masa hipofize nisu bile statistički značajno promenjene u poređenju sa odgovarajućom kontrolom (p>0.05). Karakteristike imunohistohemijski obojenih ACTH ćelija kontrolnih pacova su: lokalizacija između kapilara, zvezdast oblik sa citoplazmatičnim produžecima između drugih ćelija. Male specifične sekretorne granule raspoređene su po periferiji citoplazme. Oblik i lokalizacija ACTH ćelija životinja tretiranih Oktreotidom nisu bili značajno promenjeni u odnosu na kontrolu. Međutim, oktreotid je izazvao značajno smanjenje svih morfometrijskih parametara ovih ćelija (p>0.05). Zapremina ćelija je bila smanjena za 44%, jedara za 18%, a volumenska gustina za 31% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata može se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize mužjaka pacova.
PB  - Unija bioloških naučnih društava Jugoslavije
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Efekti oktreotida na acth ćelije hipofize mužjaka pacova
T1  - A somatostatin analogue octreotide inhibits pituitary ACHT cells in rat males
IS  - 1
VL  - 39
SP  - 25
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_67
ER  - 

@article{
author = "Milošević, Verica and Manojlović-Stojanoski, Milica and Kostić, Nada and Brkić, Branislava and Velkovski, Saško D. and Šošić-Jurjević, Branka  and Trifunović, Svetlana and Starčević, Vesna P.",
year = "2003, 2003",
abstract = "The results on the effects of synthetic somatostatins on the secretion of ACTH have been rather conflicting, but some authors reported that somatostain is involved in the regulation of ACTH secretion in rats of both sexes. The inhibitory mechanism of ACTH secretion could operate through the inhibition of CRF release from the hypothalamus. In the present study, we have investigated the effects of Octreotide, a somatostain analogue given intracerebroventricularly (i.c.v) in low doses, on morphometric characteristics of pituitary adrenocorticotropes (ACTH cells) in adult Wistar rat males. The animals were i.c.v. given three 1.0 µg doses of Octreotide dissolved in 10 µL saline every second day. The controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Body weight, as well as absolute and relative pituitary weights were not significantly changed in comparison with the corresponding controls (p>0.05). The characteristics of immunohistochemicaly labelled ACTH cells in intact rat males were as follows: localization between the capillaries stellate in shape with the cytoplasmatic processes among neighbouring cells. The nucleus follows the shape of the cell body. Small specific secretory granules were distributed mainly at the periphery of the cytoplasm. The shape, and localization of ACTH immunoreactive cells in Octreotide-treated animals were not significantly changed in comparison with the controls. Octreotide treatment significantly decreased all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 44% and 18% respectively, as compared to the controls (p<0.05). Volume densities were also significantly decreased (by 31%; p<0.05) comparing to the corresponding controls. These results indicate that Octreotide applied i.c.v. exerts a significant inhibitory effect on the immunohistochemical and morphometric characteristics of ACTH cells in rat males., Postoje veoma različita, a često i suprotstavljena mišljenja o efektima sintetskih somatostatina na lučenje adrenokortikotropnog hormona (ACTH) iz hipofize. Mogući mehanizam inhibicije lučenja ACTH je preko inhibicije lučenja CRF iz hipotalamusa. U ovom radu su ispitivani efekti intracerebroventrikularno (i.c.v) ubrizganog analoga somatostatina Oktreotida, na rast ACTH ćelija adenohipofize mužjaka Wistar pacova. Sve eksperimentalne životinje su primile tri doze od po 1.0 µg Oktreotida rastvorenog u 10 µL fiziološkog rastvora, svakog drugog dana. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani pet dana posle poslednje injekcije. ACTH ćelije su imunocitohemijski bojene metodom peroksidaza-antiperoksidaza (PAP). Telesna masa, apsolutna i relativna masa hipofize nisu bile statistički značajno promenjene u poređenju sa odgovarajućom kontrolom (p>0.05). Karakteristike imunohistohemijski obojenih ACTH ćelija kontrolnih pacova su: lokalizacija između kapilara, zvezdast oblik sa citoplazmatičnim produžecima između drugih ćelija. Male specifične sekretorne granule raspoređene su po periferiji citoplazme. Oblik i lokalizacija ACTH ćelija životinja tretiranih Oktreotidom nisu bili značajno promenjeni u odnosu na kontrolu. Međutim, oktreotid je izazvao značajno smanjenje svih morfometrijskih parametara ovih ćelija (p>0.05). Zapremina ćelija je bila smanjena za 44%, jedara za 18%, a volumenska gustina za 31% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata može se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize mužjaka pacova.",
publisher = "Unija bioloških naučnih društava Jugoslavije",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Efekti oktreotida na acth ćelije hipofize mužjaka pacova, A somatostatin analogue octreotide inhibits pituitary ACHT cells in rat males",
number = "1",
volume = "39",
pages = "25-30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_67"
}

Milošević, V., Manojlović-Stojanoski, M., Kostić, N., Brkić, B., Velkovski, S. D., Šošić-Jurjević, B., Trifunović, S.,& Starčević, V. P.. (2003). Efekti oktreotida na acth ćelije hipofize mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
Unija bioloških naučnih društava Jugoslavije., 39(1), 25-30.
https://hdl.handle.net/21.15107/rcub_ibiss_67

Milošević V, Manojlović-Stojanoski M, Kostić N, Brkić B, Velkovski SD, Šošić-Jurjević B, Trifunović S, Starčević VP. Efekti oktreotida na acth ćelije hipofize mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2003;39(1):25-30.
https://hdl.handle.net/21.15107/rcub_ibiss_67 .

Milošević, Verica, Manojlović-Stojanoski, Milica, Kostić, Nada, Brkić, Branislava, Velkovski, Saško D., Šošić-Jurjević, Branka , Trifunović, Svetlana, Starčević, Vesna P., "Efekti oktreotida na acth ćelije hipofize mužjaka pacova" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 39, no. 1 (2003):25-30,
https://hdl.handle.net/21.15107/rcub_ibiss_67 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Brkić, Branislava
AU  - Starčević, Vesna P.
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/25
AB  - The effects of chronic exposure to light of adult female Wistar rats on growth and function of pituitary adrenocorticotropes (ACTH cells) were examined. The animals were exposed to continuous light of 600 lux for 95 days, starting on day 30 of age. Control rats were kept under a 12:12 h light-dark cycle, at ambient temperature. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure and blood samples were collected for hormone analyses. In animals exposed to a chronic light-treatment all morphometric parameters measured throughout the present study i.e.: ACTH cell volume, nuclear volume and relative volume density were increased by 22% and the differences between this group and the controls were statistically significant (p<0.05). The concentration of plasma ACTH was elevated by 13% in light-exposed group in comparison with the control and this difference was statistically significant (p<0.05), as well. These findings suggest that continuous exposure to light is specifically involved in growth and secretory activity of ACTH cells of adenohypophysis of rat females.
AB  - Ispitivani su efekti dugotrajnog izlaganja ženki Wistar pacova stalnom svetlu, na adrenokortikotropne (ACTH) ćelije. Eksperimentalne životinje stare 30 dana izlagane su konstantnom svetlu (600 luksa) tokom 95 dana. Kontrolne životinje odgovarajuće starosti su držane na normalnom, 12:12 h dnevno-noćnom režimu i sobnoj temperaturi. ACTH ćelije su imunohistohemijski bojene metodom peroksidaza-antiperoksidaza (PAP), a istovremeno je sakupljana krv za određivanje koncentracije ACTH u plazmi. Životinje izložene stalnom svetlu imale su za 22% povećane sve morfometrijske parametre ACTH ćelija merene tokom ovog rada (zapremina ćelija, njihovih jedara i volumenska gustina) u odnosu na kontrole i uočene razlike su bile statistički značajne (p < 0.05). Koncentracija ACTH u plazmi je takođe bila veća za 13% (p < 0.05) u odnosu na kontrolne vrednosti. Ovi rezultati ukazuju da dugotrajno izlaganje stalnom svetlu na specifičan način stimuliše rast i sekretornu aktivnost ne samo ACTH ćelija adenohipofize već i ćelija pars intermedia ženki pacova.
T2  - Jugoslovenska medicinska biohemija
T1  - Osobine imunopozitivnih ACTH ćelija ženki pacova posle dugotrajnog izlaganja stalnom svetlu
T1  - Characteristics of the pituitary immunopositive ACTH cells in rat females after chronic exposure to constant light
IS  - 1
VL  - 22
DO  - 10.2298/JMH0301027M
SP  - 27
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_25
ER  - 

@article{
author = "Milošević, Verica and Nestorović, Nataša and Ristić, Nataša and Filipović, Branko and Brkić, Branislava and Starčević, Vesna P.",
year = "2003",
abstract = "The effects of chronic exposure to light of adult female Wistar rats on growth and function of pituitary adrenocorticotropes (ACTH cells) were examined. The animals were exposed to continuous light of 600 lux for 95 days, starting on day 30 of age. Control rats were kept under a 12:12 h light-dark cycle, at ambient temperature. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure and blood samples were collected for hormone analyses. In animals exposed to a chronic light-treatment all morphometric parameters measured throughout the present study i.e.: ACTH cell volume, nuclear volume and relative volume density were increased by 22% and the differences between this group and the controls were statistically significant (p<0.05). The concentration of plasma ACTH was elevated by 13% in light-exposed group in comparison with the control and this difference was statistically significant (p<0.05), as well. These findings suggest that continuous exposure to light is specifically involved in growth and secretory activity of ACTH cells of adenohypophysis of rat females., Ispitivani su efekti dugotrajnog izlaganja ženki Wistar pacova stalnom svetlu, na adrenokortikotropne (ACTH) ćelije. Eksperimentalne životinje stare 30 dana izlagane su konstantnom svetlu (600 luksa) tokom 95 dana. Kontrolne životinje odgovarajuće starosti su držane na normalnom, 12:12 h dnevno-noćnom režimu i sobnoj temperaturi. ACTH ćelije su imunohistohemijski bojene metodom peroksidaza-antiperoksidaza (PAP), a istovremeno je sakupljana krv za određivanje koncentracije ACTH u plazmi. Životinje izložene stalnom svetlu imale su za 22% povećane sve morfometrijske parametre ACTH ćelija merene tokom ovog rada (zapremina ćelija, njihovih jedara i volumenska gustina) u odnosu na kontrole i uočene razlike su bile statistički značajne (p < 0.05). Koncentracija ACTH u plazmi je takođe bila veća za 13% (p < 0.05) u odnosu na kontrolne vrednosti. Ovi rezultati ukazuju da dugotrajno izlaganje stalnom svetlu na specifičan način stimuliše rast i sekretornu aktivnost ne samo ACTH ćelija adenohipofize već i ćelija pars intermedia ženki pacova.",
journal = "Jugoslovenska medicinska biohemija",
title = "Osobine imunopozitivnih ACTH ćelija ženki pacova posle dugotrajnog izlaganja stalnom svetlu, Characteristics of the pituitary immunopositive ACTH cells in rat females after chronic exposure to constant light",
number = "1",
volume = "22",
doi = "10.2298/JMH0301027M",
pages = "27-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_25"
}

Milošević, V., Nestorović, N., Ristić, N., Filipović, B., Brkić, B.,& Starčević, V. P.. (2003). Osobine imunopozitivnih ACTH ćelija ženki pacova posle dugotrajnog izlaganja stalnom svetlu. in Jugoslovenska medicinska biohemija, 22(1), 27-32.
https://doi.org/10.2298/JMH0301027M
https://hdl.handle.net/21.15107/rcub_ibiss_25

Milošević V, Nestorović N, Ristić N, Filipović B, Brkić B, Starčević VP. Osobine imunopozitivnih ACTH ćelija ženki pacova posle dugotrajnog izlaganja stalnom svetlu. in Jugoslovenska medicinska biohemija. 2003;22(1):27-32.
doi:10.2298/JMH0301027M
https://hdl.handle.net/21.15107/rcub_ibiss_25 .

Milošević, Verica, Nestorović, Nataša, Ristić, Nataša, Filipović, Branko, Brkić, Branislava, Starčević, Vesna P., "Osobine imunopozitivnih ACTH ćelija ženki pacova posle dugotrajnog izlaganja stalnom svetlu" in Jugoslovenska medicinska biohemija, 22, no. 1 (2003):27-32,
https://doi.org/10.2298/JMH0301027M .,
https://hdl.handle.net/21.15107/rcub_ibiss_25 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, Branislava
AU  - Nestorović, Nataša
AU  - Velkovski, S.
AU  - Starčević, Vesna
PY  - 2001
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/458
AB  - The effects of intracerebroventricular (i.c.v.) application of a somatostatin analogue, Octreotide, on growth of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. The animals were subjected to i.c.v. administration of three 1.0μg doses of Octreotide dissolved in 10μ1 saline every second day. Controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase -antiperoxidase (PAP) immunohistochemical procedure. Octreotide treatment of rat females, significantly decreased (p<0.05) all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 52% and 11 %, respectively, as compared to the controls. The volume densities were also significantly decreased (by 47%; p<0.05) in comparison with the corresponding controls. These findings suggest that centrally administered somatostatin analogue (Octreotide) is specifically involved in the control of growth and secretory activity of ACTH cells in female rats.
AB  - Ispitivani su efekti intracerebroventrikularno (i.c.v.) ubrizganog analoga somatostatina, Oktreotida, na rast adrenokortikalnih (ACTH) ćelija adenohipofize ženki Wistar pacova. Sve eksperimentalne životinje su primile tri 1μg doze Oktreotida rastvorenog u 10μL fiziološkog rastvora, svaki drugi dan. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani petog dana po poslednjoj primljenoj dozi Oktreotida. ACTH ćelije su imunocitohemijski bojene PAP metodom. Dobijeni rezultati pokazuju da su statistički značajno smanjeni svi morfometrijski parametri tretiranih ženki pacova u odnosu na odgovarajuću kontrolu. Zapremina ćelija je smanjena za 52%, jedara za 11%, a volumenska gustina za 47% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata maze se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize ženki pacova.
T2  - Acta veterinaria
T1  - Efekti oktreotida na ACTH ćelije hipofize ženki pacova
T1  - The effects of centrally applied octreotide on ACTH cells in the female rat
IS  - 5-6
VL  - 51
SP  - 283
EP  - 290
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_458
ER  - 

@article{
author = "Milošević, Verica and Brkić, Branislava and Nestorović, Nataša and Velkovski, S. and Starčević, Vesna",
year = "2001, 2001",
abstract = "The effects of intracerebroventricular (i.c.v.) application of a somatostatin analogue, Octreotide, on growth of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. The animals were subjected to i.c.v. administration of three 1.0μg doses of Octreotide dissolved in 10μ1 saline every second day. Controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase -antiperoxidase (PAP) immunohistochemical procedure. Octreotide treatment of rat females, significantly decreased (p<0.05) all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 52% and 11 %, respectively, as compared to the controls. The volume densities were also significantly decreased (by 47%; p<0.05) in comparison with the corresponding controls. These findings suggest that centrally administered somatostatin analogue (Octreotide) is specifically involved in the control of growth and secretory activity of ACTH cells in female rats., Ispitivani su efekti intracerebroventrikularno (i.c.v.) ubrizganog analoga somatostatina, Oktreotida, na rast adrenokortikalnih (ACTH) ćelija adenohipofize ženki Wistar pacova. Sve eksperimentalne životinje su primile tri 1μg doze Oktreotida rastvorenog u 10μL fiziološkog rastvora, svaki drugi dan. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani petog dana po poslednjoj primljenoj dozi Oktreotida. ACTH ćelije su imunocitohemijski bojene PAP metodom. Dobijeni rezultati pokazuju da su statistički značajno smanjeni svi morfometrijski parametri tretiranih ženki pacova u odnosu na odgovarajuću kontrolu. Zapremina ćelija je smanjena za 52%, jedara za 11%, a volumenska gustina za 47% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata maze se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize ženki pacova.",
journal = "Acta veterinaria",
title = "Efekti oktreotida na ACTH ćelije hipofize ženki pacova, The effects of centrally applied octreotide on ACTH cells in the female rat",
number = "5-6",
volume = "51",
pages = "283-290",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_458"
}

Milošević, V., Brkić, B., Nestorović, N., Velkovski, S.,& Starčević, V.. (2001). Efekti oktreotida na ACTH ćelije hipofize ženki pacova. in Acta veterinaria, 51(5-6), 283-290.
https://hdl.handle.net/21.15107/rcub_ibiss_458

Milošević V, Brkić B, Nestorović N, Velkovski S, Starčević V. Efekti oktreotida na ACTH ćelije hipofize ženki pacova. in Acta veterinaria. 2001;51(5-6):283-290.
https://hdl.handle.net/21.15107/rcub_ibiss_458 .

Milošević, Verica, Brkić, Branislava, Nestorović, Nataša, Velkovski, S., Starčević, Vesna, "Efekti oktreotida na ACTH ćelije hipofize ženki pacova" in Acta veterinaria, 51, no. 5-6 (2001):283-290,
https://hdl.handle.net/21.15107/rcub_ibiss_458 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
AU  - Brkić, Branislava
AU  - Lovren, Mirjana B.
AU  - Starčević, Vesna P.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1829
AB  - The effects of intracerebroventricularly administered somatostatin (SRIH-14 or -28) on growth and function of pituitary thyrotropes (TSH-cells) were examined in adult male Wistar rats. The animals were implanted with an intracerebroventricular cannula and after a recovery period, administered three 1 mug doses of SRIH-14 or -28 dissolved in 5 mul saline every second day. Controls were treated in the same way with the same volume of saline only. TSH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. Blood samples were collected for hormone (TSH) analyses 5 days after the last injection. Both SRIH-treatments significantly decreased (p < 0.05) all morphometric parameters obtained for TSH-cells in comparison with controls. The volume of TSH-cells decreased by 27%, nuclei by 44% and volume density by 33% in animals treated with SRIH-14. In animals treated with SRIH-28, these parameters were also significantly decreased (p < 0.05) (22%, 31%, and 25% respectively) compared to control rats. Serum concentrations of TSH were significantly decreased (p < 0.05) by 15% in SRIH-14- and by 12% in SRIH-28-treated animals in comparison with the controls. These observations suggest that centrally administered SRIH-14 or -28 is specifically involved in the control of growth and secretory activity of TSH cells.
T2  - Histochemical Journal
T1  - Effect of centrally administered somatostatin on pituitary thyrotropes in male rats
IS  - 9
VL  - 32
EP  - 569
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1829
ER  - 

@article{
author = "Milošević, Verica and Sekulić, Milka I. and Brkić, Branislava and Lovren, Mirjana B. and Starčević, Vesna P.",
year = "2000",
abstract = "The effects of intracerebroventricularly administered somatostatin (SRIH-14 or -28) on growth and function of pituitary thyrotropes (TSH-cells) were examined in adult male Wistar rats. The animals were implanted with an intracerebroventricular cannula and after a recovery period, administered three 1 mug doses of SRIH-14 or -28 dissolved in 5 mul saline every second day. Controls were treated in the same way with the same volume of saline only. TSH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. Blood samples were collected for hormone (TSH) analyses 5 days after the last injection. Both SRIH-treatments significantly decreased (p < 0.05) all morphometric parameters obtained for TSH-cells in comparison with controls. The volume of TSH-cells decreased by 27%, nuclei by 44% and volume density by 33% in animals treated with SRIH-14. In animals treated with SRIH-28, these parameters were also significantly decreased (p < 0.05) (22%, 31%, and 25% respectively) compared to control rats. Serum concentrations of TSH were significantly decreased (p < 0.05) by 15% in SRIH-14- and by 12% in SRIH-28-treated animals in comparison with the controls. These observations suggest that centrally administered SRIH-14 or -28 is specifically involved in the control of growth and secretory activity of TSH cells.",
journal = "Histochemical Journal",
title = "Effect of centrally administered somatostatin on pituitary thyrotropes in male rats",
number = "9",
volume = "32",
pages = "569",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1829"
}

Milošević, V., Sekulić, M. I., Brkić, B., Lovren, M. B.,& Starčević, V. P.. (2000). Effect of centrally administered somatostatin on pituitary thyrotropes in male rats. in Histochemical Journal, 32(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1829

Milošević V, Sekulić MI, Brkić B, Lovren MB, Starčević VP. Effect of centrally administered somatostatin on pituitary thyrotropes in male rats. in Histochemical Journal. 2000;32(9):null-569.
https://hdl.handle.net/21.15107/rcub_ibiss_1829 .

Milošević, Verica, Sekulić, Milka I., Brkić, Branislava, Lovren, Mirjana B., Starčević, Vesna P., "Effect of centrally administered somatostatin on pituitary thyrotropes in male rats" in Histochemical Journal, 32, no. 9 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1829 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Lovren, Mirjana
AU  - Sekulić, Milka
AU  - Brkić, Branislava
AU  - Velkovski, S.
AU  - Starčević, Vesna
PY  - 2000
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/453
AB  - This study examined the effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 orSRIH-28) on growth and function of pituitary somatotropes (GH cells). Female rats were subjected to intracerebroventricular administration of three 1g doses of SRIH-14 or SRIH-28 every second day. Blood samples were collected for hormone analyses and pituitaries were removed for histological and morphometric evaluation, five days after the last injection. Compared to control animals, SRIH treatment decreased (p<0.05) all morphometric measurements obtained for GH cells. The concentration of serum growth hormone (GH) in SRIH-14- or SRIH-28-treated groups was lower (p<0.05) by 69% and by 61% respectively, in comparison to control rats. These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH cells. Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter the systemic effects of GH.
AB  - Ispitivani su efekti intracerebroventrikularno (i.c.v.) ubrizganih somatostat-ina (SRIH-14 ili SRIH-28) na rast i funkciju somatotropnih ćelija hipofize ženki pacova. Sve eksperimentalne životinje su primale po 1μg SRIH-14 ili SRIH-28 rastvorenog u 5μL fiziološkog rastvora, svaki drugi dan. Kontrole su na isti način injecirane fiziološkim rastvorom. Životinje su žrtvovane petog dana po poslednjoj primljenoj dozi. Krv je sakupljana za određivanje koncentracije hormona rasta (GH) u serumu, a hipofize su pripremane za histološku i morfometrijsku analizu. Dobijeni rezultati pokazuju da SRIH tretirane životinje pokazuju značajno smanjenje (p<0.05) svih ispitivanih morfometrijskih parametara (volumen ćelija i jedara, volumenska gustina) u odnosu na kontrolu. Koncentracija GH u serumu SRIH-14 i SRIF-28 tretiranih ženki takodje je značajno smanjena(p<0.05)za69%odnosno 61% u poređenju sa kontrolnim ženkama. Na osnovu ovako dobijenih rezultata može se zaključiti da oba aplikovana somatostatina inhibiraju rast i sekretornu aktivnost somatotropnih ćelija hipofize.
T2  - Acta veterinaria
T1  - Morfometrijske i funkcionalne promene somatotropnih ćelija hipofize ženki pacova posle centralne aplikacije somatostatina
T1  - Morphometric and functional changes of female rat pituitary somatotropes after central application of somatostatin
IS  - 2-3
VL  - 50
SP  - 93
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_453
ER  - 

@article{
author = "Milošević, Verica and Lovren, Mirjana and Sekulić, Milka and Brkić, Branislava and Velkovski, S. and Starčević, Vesna",
year = "2000, 2000",
abstract = "This study examined the effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 orSRIH-28) on growth and function of pituitary somatotropes (GH cells). Female rats were subjected to intracerebroventricular administration of three 1g doses of SRIH-14 or SRIH-28 every second day. Blood samples were collected for hormone analyses and pituitaries were removed for histological and morphometric evaluation, five days after the last injection. Compared to control animals, SRIH treatment decreased (p<0.05) all morphometric measurements obtained for GH cells. The concentration of serum growth hormone (GH) in SRIH-14- or SRIH-28-treated groups was lower (p<0.05) by 69% and by 61% respectively, in comparison to control rats. These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH cells. Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter the systemic effects of GH., Ispitivani su efekti intracerebroventrikularno (i.c.v.) ubrizganih somatostat-ina (SRIH-14 ili SRIH-28) na rast i funkciju somatotropnih ćelija hipofize ženki pacova. Sve eksperimentalne životinje su primale po 1μg SRIH-14 ili SRIH-28 rastvorenog u 5μL fiziološkog rastvora, svaki drugi dan. Kontrole su na isti način injecirane fiziološkim rastvorom. Životinje su žrtvovane petog dana po poslednjoj primljenoj dozi. Krv je sakupljana za određivanje koncentracije hormona rasta (GH) u serumu, a hipofize su pripremane za histološku i morfometrijsku analizu. Dobijeni rezultati pokazuju da SRIH tretirane životinje pokazuju značajno smanjenje (p<0.05) svih ispitivanih morfometrijskih parametara (volumen ćelija i jedara, volumenska gustina) u odnosu na kontrolu. Koncentracija GH u serumu SRIH-14 i SRIF-28 tretiranih ženki takodje je značajno smanjena(p<0.05)za69%odnosno 61% u poređenju sa kontrolnim ženkama. Na osnovu ovako dobijenih rezultata može se zaključiti da oba aplikovana somatostatina inhibiraju rast i sekretornu aktivnost somatotropnih ćelija hipofize.",
journal = "Acta veterinaria",
title = "Morfometrijske i funkcionalne promene somatotropnih ćelija hipofize ženki pacova posle centralne aplikacije somatostatina, Morphometric and functional changes of female rat pituitary somatotropes after central application of somatostatin",
number = "2-3",
volume = "50",
pages = "93-101",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_453"
}

Milošević, V., Lovren, M., Sekulić, M., Brkić, B., Velkovski, S.,& Starčević, V.. (2000). Morfometrijske i funkcionalne promene somatotropnih ćelija hipofize ženki pacova posle centralne aplikacije somatostatina. in Acta veterinaria, 50(2-3), 93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_453

Milošević V, Lovren M, Sekulić M, Brkić B, Velkovski S, Starčević V. Morfometrijske i funkcionalne promene somatotropnih ćelija hipofize ženki pacova posle centralne aplikacije somatostatina. in Acta veterinaria. 2000;50(2-3):93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_453 .

Milošević, Verica, Lovren, Mirjana, Sekulić, Milka, Brkić, Branislava, Velkovski, S., Starčević, Vesna, "Morfometrijske i funkcionalne promene somatotropnih ćelija hipofize ženki pacova posle centralne aplikacije somatostatina" in Acta veterinaria, 50, no. 2-3 (2000):93-101,
https://hdl.handle.net/21.15107/rcub_ibiss_453 .