TY  - JOUR
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Dragićević, Milan
AU  - Kankare, Maaria
AU  - Wallace, Megan A.
AU  - Savić Veselinović, Marija
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://www.eje.cz/doi/10.14411/eje.2022.011.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4872
AB  - Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.
PB  - České Budějovice: Czech Academy of Sciences
T2  - European Journal of Entomology
T1  - Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe
VL  - 119
DO  - 10.14411/eje.2022.011
SP  - 99
EP  - 110
ER  - 

@article{
author = "Erić, Pavle and Stamenković-Radak, Marina and Dragićević, Milan and Kankare, Maaria and Wallace, Megan A. and Savić Veselinović, Marija and Jelić, Mihailo",
year = "2022",
abstract = "Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.",
publisher = "České Budějovice: Czech Academy of Sciences",
journal = "European Journal of Entomology",
title = "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe",
volume = "119",
doi = "10.14411/eje.2022.011",
pages = "99-110"
}

Erić, P., Stamenković-Radak, M., Dragićević, M., Kankare, M., Wallace, M. A., Savić Veselinović, M.,& Jelić, M.. (2022). Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology
České Budějovice: Czech Academy of Sciences., 119, 99-110.
https://doi.org/10.14411/eje.2022.011

Erić P, Stamenković-Radak M, Dragićević M, Kankare M, Wallace MA, Savić Veselinović M, Jelić M. Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology. 2022;119:99-110.
doi:10.14411/eje.2022.011 .

Erić, Pavle, Stamenković-Radak, Marina, Dragićević, Milan, Kankare, Maaria, Wallace, Megan A., Savić Veselinović, Marija, Jelić, Mihailo, "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe" in European Journal of Entomology, 119 (2022):99-110,
https://doi.org/10.14411/eje.2022.011 . .

TY  - JOUR
AU  - Wallace, Megan A
AU  - Coffman, Kelsey A
AU  - Gilbert, Clément
AU  - Ravindran, Sanjana
AU  - Albery, Gregory F
AU  - Abbott, Jessica
AU  - Argyridou, Eliza
AU  - Bellosta, Paola
AU  - Betancourt, Andrea J
AU  - Colinet, Hervé
AU  - Eric, Katarina
AU  - Glaser-Schmitt, Amanda
AU  - Grath, Sonja
AU  - Jelić, Mihailo
AU  - Kankare, Maaria
AU  - Kozeretska, Iryna
AU  - Loeschcke, Volker
AU  - Montchamp-Moreau, Catherine
AU  - Ometto, Lino
AU  - Onder, Banu Sebnem
AU  - Orengo, Dorcas J
AU  - Parsch, John
AU  - Pascual, Marta
AU  - Patenković, Aleksandra
AU  - Puerma, Eva
AU  - Ritchie, Michael G
AU  - Rota-Stabelli, Omar
AU  - Schou, Mads Fristrup
AU  - Serga, Svitlana V
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
AU  - Savić Veselinović, Marija
AU  - Vieira, Jorge
AU  - Vieira, Cristina P
AU  - Kapun, Martin
AU  - Flatt, Thomas
AU  - González, Josefa
AU  - Staubach, Fabian
AU  - Obbard, Darren J
PY  - 2021
UR  - https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veab031/6207981
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4184
AB  - Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
T2  - Virus Evolution
T2  - Virus Evolution
T1  - The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe
DO  - 10.1093/ve/veab031
ER  - 

@article{
author = "Wallace, Megan A and Coffman, Kelsey A and Gilbert, Clément and Ravindran, Sanjana and Albery, Gregory F and Abbott, Jessica and Argyridou, Eliza and Bellosta, Paola and Betancourt, Andrea J and Colinet, Hervé and Eric, Katarina and Glaser-Schmitt, Amanda and Grath, Sonja and Jelić, Mihailo and Kankare, Maaria and Kozeretska, Iryna and Loeschcke, Volker and Montchamp-Moreau, Catherine and Ometto, Lino and Onder, Banu Sebnem and Orengo, Dorcas J and Parsch, John and Pascual, Marta and Patenković, Aleksandra and Puerma, Eva and Ritchie, Michael G and Rota-Stabelli, Omar and Schou, Mads Fristrup and Serga, Svitlana V and Stamenković-Radak, Marina and Tanasković, Marija and Savić Veselinović, Marija and Vieira, Jorge and Vieira, Cristina P and Kapun, Martin and Flatt, Thomas and González, Josefa and Staubach, Fabian and Obbard, Darren J",
year = "2021",
abstract = "Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.",
journal = "Virus Evolution, Virus Evolution",
title = "The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe",
doi = "10.1093/ve/veab031"
}

Wallace, M. A., Coffman, K. A., Gilbert, C., Ravindran, S., Albery, G. F., Abbott, J., Argyridou, E., Bellosta, P., Betancourt, A. J., Colinet, H., Eric, K., Glaser-Schmitt, A., Grath, S., Jelić, M., Kankare, M., Kozeretska, I., Loeschcke, V., Montchamp-Moreau, C., Ometto, L., Onder, B. S., Orengo, D. J., Parsch, J., Pascual, M., Patenković, A., Puerma, E., Ritchie, M. G., Rota-Stabelli, O., Schou, M. F., Serga, S. V., Stamenković-Radak, M., Tanasković, M., Savić Veselinović, M., Vieira, J., Vieira, C. P., Kapun, M., Flatt, T., González, J., Staubach, F.,& Obbard, D. J.. (2021). The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe. in Virus Evolution.
https://doi.org/10.1093/ve/veab031

Wallace MA, Coffman KA, Gilbert C, Ravindran S, Albery GF, Abbott J, Argyridou E, Bellosta P, Betancourt AJ, Colinet H, Eric K, Glaser-Schmitt A, Grath S, Jelić M, Kankare M, Kozeretska I, Loeschcke V, Montchamp-Moreau C, Ometto L, Onder BS, Orengo DJ, Parsch J, Pascual M, Patenković A, Puerma E, Ritchie MG, Rota-Stabelli O, Schou MF, Serga SV, Stamenković-Radak M, Tanasković M, Savić Veselinović M, Vieira J, Vieira CP, Kapun M, Flatt T, González J, Staubach F, Obbard DJ. The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe. in Virus Evolution. 2021;.
doi:10.1093/ve/veab031 .

Wallace, Megan A, Coffman, Kelsey A, Gilbert, Clément, Ravindran, Sanjana, Albery, Gregory F, Abbott, Jessica, Argyridou, Eliza, Bellosta, Paola, Betancourt, Andrea J, Colinet, Hervé, Eric, Katarina, Glaser-Schmitt, Amanda, Grath, Sonja, Jelić, Mihailo, Kankare, Maaria, Kozeretska, Iryna, Loeschcke, Volker, Montchamp-Moreau, Catherine, Ometto, Lino, Onder, Banu Sebnem, Orengo, Dorcas J, Parsch, John, Pascual, Marta, Patenković, Aleksandra, Puerma, Eva, Ritchie, Michael G, Rota-Stabelli, Omar, Schou, Mads Fristrup, Serga, Svitlana V, Stamenković-Radak, Marina, Tanasković, Marija, Savić Veselinović, Marija, Vieira, Jorge, Vieira, Cristina P, Kapun, Martin, Flatt, Thomas, González, Josefa, Staubach, Fabian, Obbard, Darren J, "The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe" in Virus Evolution (2021),
https://doi.org/10.1093/ve/veab031 . .