TY  - JOUR
AU  - Džamić, Ana M.
AU  - Nikolic, B. J.
AU  - Giweli, A. A.
AU  - Mitic-Culafic, D. S.
AU  - Soković, Marina
AU  - Ristic, M. S.
AU  - Knežević-Vukčević, Jelena
AU  - Marin, P. D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1922
AB  - AimsIn the present work, the Libyan wild-growing Thymus capitatus
   essential oil (EO) was evaluated for its biological properties.
   Methods and ResultsCarvacrol (6819\%) and thymol (1229\%) were found to
   be the main compounds of the oil. Antioxidant properties, determined by
   2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were
   119, 403 and 105gml(-1) for oil, thymol and carvacrol respectively.
   Microdilution method showed strong antibacterial and especially
   antifungal potential. Tetrazolium (MTT) colorimetric assay indicated
   moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29
   (IC50=30-150gml(-1)). In adhesion-inhibition assay oil and main
   compounds reduced adhesion of Escherichia coli and Listeria
   monocytogenes on colon cells HT-29 (51 and 39\% of inhibition against
   L.monocytogenes and E.coli respectively).
   ConclusionsEssential oil of Th.capitatus showed moderate cytotoxic
   activity, together with excellent antimicrobial effect, in particular
   against fungi, and significant potential to reduce pathogen colonization
   in colon.
   Significance and Impact of the StudyThis is the first report that EO of
   Th.capitatus could protect against colonization of pathogens to colon
   epithelium. Thymus capitatus from Libya should be recognized as possible
   new source of natural antioxidants, antimicrobials as well as possible
   source of new chemotherapeutics.
T2  - Journal of Applied Microbiology
T1  - Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties
IS  - 2
VL  - 119
DO  - 10.1111/jam.12864
SP  - 389
EP  - 399
ER  - 

@article{
author = "Džamić, Ana M. and Nikolic, B. J. and Giweli, A. A. and Mitic-Culafic, D. S. and Soković, Marina and Ristic, M. S. and Knežević-Vukčević, Jelena and Marin, P. D.",
year = "2015",
abstract = "AimsIn the present work, the Libyan wild-growing Thymus capitatus
   essential oil (EO) was evaluated for its biological properties.
   Methods and ResultsCarvacrol (6819\%) and thymol (1229\%) were found to
   be the main compounds of the oil. Antioxidant properties, determined by
   2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were
   119, 403 and 105gml(-1) for oil, thymol and carvacrol respectively.
   Microdilution method showed strong antibacterial and especially
   antifungal potential. Tetrazolium (MTT) colorimetric assay indicated
   moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29
   (IC50=30-150gml(-1)). In adhesion-inhibition assay oil and main
   compounds reduced adhesion of Escherichia coli and Listeria
   monocytogenes on colon cells HT-29 (51 and 39\% of inhibition against
   L.monocytogenes and E.coli respectively).
   ConclusionsEssential oil of Th.capitatus showed moderate cytotoxic
   activity, together with excellent antimicrobial effect, in particular
   against fungi, and significant potential to reduce pathogen colonization
   in colon.
   Significance and Impact of the StudyThis is the first report that EO of
   Th.capitatus could protect against colonization of pathogens to colon
   epithelium. Thymus capitatus from Libya should be recognized as possible
   new source of natural antioxidants, antimicrobials as well as possible
   source of new chemotherapeutics.",
journal = "Journal of Applied Microbiology",
title = "Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties",
number = "2",
volume = "119",
doi = "10.1111/jam.12864",
pages = "389-399"
}

Džamić, A. M., Nikolic, B. J., Giweli, A. A., Mitic-Culafic, D. S., Soković, M., Ristic, M. S., Knežević-Vukčević, J.,& Marin, P. D.. (2015). Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties. in Journal of Applied Microbiology, 119(2), 389-399.
https://doi.org/10.1111/jam.12864

Džamić AM, Nikolic BJ, Giweli AA, Mitic-Culafic DS, Soković M, Ristic MS, Knežević-Vukčević J, Marin PD. Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties. in Journal of Applied Microbiology. 2015;119(2):389-399.
doi:10.1111/jam.12864 .

Džamić, Ana M., Nikolic, B. J., Giweli, A. A., Mitic-Culafic, D. S., Soković, Marina, Ristic, M. S., Knežević-Vukčević, Jelena, Marin, P. D., "Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties" in Journal of Applied Microbiology, 119, no. 2 (2015):389-399,
https://doi.org/10.1111/jam.12864 . .