@article{
author = "Dunđerski, Jadranka S. and Vidović, Stojko and Matić, Gordana",
year = "2003",
abstract = "The aim of the present study was to examine the influence of dexamethasone on the levels of heat shock protein Hsp70 and glucocorticoid hormones receptor as well as on the interaction of these two proteins in the liver cytosol and nuclei of unstressed and rats exposed to whole body hyperthermic stress. The results, obtained by quantitative immunoblotting, have shown that dexamethasone provoked a reduction of Hsp70 basal level and an increase in its stress-induced level in the nuclei, supporting the idea that this hormone may be a factor included in the regulation of Hsp70 level both under normal and stress conditions. The cytosolic reduction and nuclear elevation of the glucocorticoid hormones receptor level by dexamethasone were also observed. Co-immunopurification of Hsp70 and glucocorticoid hormones receptor has revealed that the changes of cytosolic and nuclear levels of the two examined proteins resulted in the changes of their interaction within the respective cellular compartments. Thus, 41 °C heat stress was shown to cause at least two-fold elevation of Hsp70/GR ratio within the glucocorticoid hormones receptor heterocomplexes both in the presence and in the absence of dexamethasone. The results support the view that glucocorticoid hormones signaling pathway and heat shock system are interrelated., Cilj ovog rada bio je da se ispita uticaj deksametazona na koncentraciju proteina toplotnog stresa Hsp70 i glukokortikoidnog receptora (GR), kao i na interakciju ovih proteina, u citosolu i jedrima jetre nestresiranih pacova i pacova izloženih hipertermijskom stresu. Rezultati, dobijeni kvantitativnim imunoblotom, su pokazali da deksametazon dovodi do smanjenja bazalne i povećanja stresom indukovane koncentracije Hsp70 u jedrima, što ukazuje na mogućnost da je ovaj hormon jedan od faktora uključenih u regulaciju nivoa Hsp70 i u normalnim i u uslovima stresa. Zapaženo je i to da deksametazon uzrokuje smanjenje citosolne i povećanje jedarne koncentracije GR. Ko-imunopurifikacija Hsp70 i GR je pokazala da su promene u citosolnim i jedarnim koncentracijama ovih proteina praćene i promenama u njihovoj interakciji u odgovarajućim ćelijskim odeljcima. Tako, nađeno je da hipertermijski stres (41 °C) dovodi do najmanje dvostrukog povećanja odnosa Hsp70/GR u receptorskim heterokompleksima kako u prisustvu, tako i u odsustvu deksametazona. Rezultati podržavaju pretpostavku da su signalni putevi koje započinju glukokortikoidni hormoni i toplotni stres međusobno povezani.",
journal = "Jugoslovenska medicinska biohemija",
title = "Uticaj deksametazona na koncentraciju i interakciju Hsp70 sa glukokortikoidnim receptorom u jetri nestresiranih i pacova izloženih toplotnom stresu, The influence of dexamethasone on Hsp70 level and association with glucocorticoid receptor in the liver of unstressed and heat-stressed rats",
number = "1",
volume = "22",
pages = "19-26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_24"
}
