TY  - CONF
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4368
AB  - In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4368
ER  - 

@conference{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2019",
abstract = "In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4368"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Tešević, V., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2019). The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368

Nenadić M, Soković M, Glamočlija J, Ćirić A, Tešević V, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):43,
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
AU  - Vujisić, Ljubodrag
AU  - Nenadić, Marija
AU  - Krstić, Gordana
AU  - Perić Mataruga, Vesna
AU  - Milosavljević, Slobodan
AU  - Antić, Dragan
AU  - Mandić, Boris
AU  - Petković, Matija
AU  - Vučković, Ivan
AU  - Marković, Đorđe
AU  - Vrbica, Maja
AU  - Ćurčić, Božidar
AU  - Makarov, Slobodan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1947
AB  - Three adult cave-dwelling ground beetle species were induced to
   discharge secretions of their pygidial glands into vials.
   Dichloromethane extraction was used to obtain the secretions. In total,
   42 compounds were identified by GC/MS analysis. Pheggomisetes ninae
   contained 32 glandular compounds, Laemostenus (Pristonychus) punctatus
   13, whereas Duvalius (Paraduvalius) milutini had nine compounds.
   Caproic, oleic, palmitic, and stearic acids were present in the samples
   of all analyzed species. Undecane was predominant in the extract of L.
   punctatus. Palmitic acid was the major component in the secretion of D.
   milutini. Finally, the most abundant compounds in P. ninae secretion
   were heptacosene and nonacosadienes. Herein, we present the first data
   on the identification of pygidial gland secretion components in both
   troglophilous and troglobite cave-dwelling ground beetles. Some
   compounds are reported for the first time in the secretions of ground
   beetles and other higher or lower taxa. The adaptation to underground
   life has not led to a reduction or changes in the chemical defense
   mechanism in the analyzed troglophilous and troglobitic Platyninae and
   Trechinae taxa.
PB  - Springer New York LLC
T2  - Journal of Chemical Ecology
T1  - Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence
IS  - 6
VL  - 41
DO  - 10.1007/s10886-015-0593-7
SP  - 533
EP  - 539
ER  - 

@article{
author = "Vesović, Nikola and Ćurčić, Srećko and Vujisić, Ljubodrag and Nenadić, Marija and Krstić, Gordana and Perić Mataruga, Vesna and Milosavljević, Slobodan and Antić, Dragan and Mandić, Boris and Petković, Matija and Vučković, Ivan and Marković, Đorđe and Vrbica, Maja and Ćurčić, Božidar and Makarov, Slobodan",
year = "2015",
abstract = "Three adult cave-dwelling ground beetle species were induced to
   discharge secretions of their pygidial glands into vials.
   Dichloromethane extraction was used to obtain the secretions. In total,
   42 compounds were identified by GC/MS analysis. Pheggomisetes ninae
   contained 32 glandular compounds, Laemostenus (Pristonychus) punctatus
   13, whereas Duvalius (Paraduvalius) milutini had nine compounds.
   Caproic, oleic, palmitic, and stearic acids were present in the samples
   of all analyzed species. Undecane was predominant in the extract of L.
   punctatus. Palmitic acid was the major component in the secretion of D.
   milutini. Finally, the most abundant compounds in P. ninae secretion
   were heptacosene and nonacosadienes. Herein, we present the first data
   on the identification of pygidial gland secretion components in both
   troglophilous and troglobite cave-dwelling ground beetles. Some
   compounds are reported for the first time in the secretions of ground
   beetles and other higher or lower taxa. The adaptation to underground
   life has not led to a reduction or changes in the chemical defense
   mechanism in the analyzed troglophilous and troglobitic Platyninae and
   Trechinae taxa.",
publisher = "Springer New York LLC",
journal = "Journal of Chemical Ecology",
title = "Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence",
number = "6",
volume = "41",
doi = "10.1007/s10886-015-0593-7",
pages = "533-539"
}

Vesović, N., Ćurčić, S., Vujisić, L., Nenadić, M., Krstić, G., Perić Mataruga, V., Milosavljević, S., Antić, D., Mandić, B., Petković, M., Vučković, I., Marković, Đ., Vrbica, M., Ćurčić, B.,& Makarov, S.. (2015). Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence. in Journal of Chemical Ecology
Springer New York LLC., 41(6), 533-539.
https://doi.org/10.1007/s10886-015-0593-7

Vesović N, Ćurčić S, Vujisić L, Nenadić M, Krstić G, Perić Mataruga V, Milosavljević S, Antić D, Mandić B, Petković M, Vučković I, Marković Đ, Vrbica M, Ćurčić B, Makarov S. Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence. in Journal of Chemical Ecology. 2015;41(6):533-539.
doi:10.1007/s10886-015-0593-7 .

Vesović, Nikola, Ćurčić, Srećko, Vujisić, Ljubodrag, Nenadić, Marija, Krstić, Gordana, Perić Mataruga, Vesna, Milosavljević, Slobodan, Antić, Dragan, Mandić, Boris, Petković, Matija, Vučković, Ivan, Marković, Đorđe, Vrbica, Maja, Ćurčić, Božidar, Makarov, Slobodan, "Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence" in Journal of Chemical Ecology, 41, no. 6 (2015):533-539,
https://doi.org/10.1007/s10886-015-0593-7 . .