TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš
AU  - Milojković-Opsenica, Dušanka
AU  - Rimac, Hrvoje
AU  - Vuković Rodriguez, Jadranka
AU  - Vlainić, Josipa
AU  - Brlek-Gorski, Diana
AU  - Kosalec, Ivan
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/4/492
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4960
AB  - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time-kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above-described methods led to further activity-guided fractionation of water and methanol extracts using bioautography coupled with UHPLC-LTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl-shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl-shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
IS  - 4
VL  - 11
DO  - 10.3390/antibiotics11040492
SP  - 492
ER  - 

@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek-Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time-kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above-described methods led to further activity-guided fractionation of water and methanol extracts using bioautography coupled with UHPLC-LTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl-shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl-shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
number = "4",
volume = "11",
doi = "10.3390/antibiotics11040492",
pages = "492"
}

Ljoljić Bilić, V., Gašić, U., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek-Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Antibiotics
Basel: MDPI., 11(4), 492.
https://doi.org/10.3390/antibiotics11040492

Ljoljić Bilić V, Gašić U, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek-Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Antibiotics. 2022;11(4):492.
doi:10.3390/antibiotics11040492 .

Ljoljić Bilić, Vanja, Gašić, Uroš, Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek-Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Antibiotics, 11, no. 4 (2022):492,
https://doi.org/10.3390/antibiotics11040492 . .