TY  - JOUR
AU  - Bogdanović, Višnja
AU  - Slavić, Marija
AU  - Mrđanović, Jasminka
AU  - Šolajić, Slavica
AU  - Đorđević, Aleksandar
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/385
AB  - Eukaryotic cell survives in predominantly reduced conditions. Homeostasis of cellular redox system is an imperative of cell surviving and its normal metabolism. ROS are well recognized for playing a dual role as both deleterious and beneficial species, since they can be either harmful or beneficial to living systems. These species are mutagenic compounds known to lead to DNA damage, favor cell transformation, and contribute to the development of a variety of malignant diseases. All the effects of oxidants are influenced by the cellular antioxidant defenses. This multilayer system consists of low molecular weight components and several antioxidant enzymes. Superoxide dismutases (SODs) are the only enzymes dismuting superoxide radicals. Mitomycin C, a cross-linking agent, demonstrated genotoxicity in all in vitro and in vivo test systems in mammalian cells and animals. Water-soluble fullerenes are well known as cytotoxic agents for many cell lines in vitro. At the other side, fullerenols are good free radical scavengers and antioxidants both in vitro and in vivo. This paper investigates the effects of fullerenol on survival and fullerenol/ /mytomicine (MMC) treatment on superoxide-dismutase (SOD) activity in CHO-K1 cells. Samples were treated 3 and 24 h with fullerenol (C60(OH)24) at concentration range 0.01-0.5 mg/mL and survival was monitored with dye exclusion test (DET). The activity of total SOD was estimated in samples treated with chosen concentrations of fullerenol and MMC (0.5 and 0.1 mg/mL) after 3 and 24 h of cell incubation. Increasing of C60(OH)24 concentration leads to decreasing of percent of surviving cells 3 and 24 h after incubation. The activity of total SOD enhanced with higher concentration of fullerenol, while decreased in the highest concentration at both experimental points. In samples treated with MMC, as well as in samples treated with fullerenol (0.0625 mg/mL) + MMC was noticed boost in total SOD activity in comparison with controls. Treatment with fullerenol decreased SOD activity in rest of samples treated with MMC. Decreased activity of superoxide-dismutase in almost all samples treated with fullerenol and MMC might be contributed to antioxidative properties of fullerenol. Increased enzyme level at concentration of 0.0625 mg/mL may be due to its prooxidative activity.
AB  - U ovom radu ispitivani su efekti fulerenola (C60(OH)24) na preživljavanje, kao i tretmana fulerenolom i mitomicinom c (MMC) na aktivnost ukupne superoksid-dismutaze u CHO-K1 (ovarijalnih ćelija hrčka) ćelijskoj liniji. U uzorcima ćelija tretiranim fulerenolom koncentracija 0,01-0,5 mg/mL, praćeno je preživljavanje testom odbacivanja boje (DET) u 3 i 24 h tretmanu. Aktivnost superoksid-dismutaze (SOD) merena je u uzorcima tretiranim fulerenolom izabranih koncentracija i mitomicinom c (0,5 i 0,1 mg/mL) nakon 3 i 24 h. Sa porastom koncentracije (C60(OH)24) opada procenat preživelih ćelija tokom 3 i 24 h. Aktivnost SOD raste sa porastom koncentracije fulerenola i u najvećoj koncentraciji opada u obe vremenske tačke eksperimenta. U uzorcima tretiranim fulerenolom i MMC došlo je do smanjenja aktivnosti SOD, izuzev pri koncentraciji fulerenola od 0,0625 mg/mL, kada je zapažen porast aktivnosti SOD u odnosu na kontrolne grupe.
T2  - Hemijska industrija
T1  - Aktivnost superoksid-dismutaze u animalnoj ćelijskoj kulturi CHO-K1 nakon tretmana fulerenolom i mitomicinom C
T1  - The activity of superoxide-dismutase in animal cell culture CHO-K1 after treatment with fullerenol and mytomicine C
IS  - 3
VL  - 63
SP  - 143
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_385
ER  - 

@article{
author = "Bogdanović, Višnja and Slavić, Marija and Mrđanović, Jasminka and Šolajić, Slavica and Đorđević, Aleksandar",
year = "2009, 2009",
abstract = "Eukaryotic cell survives in predominantly reduced conditions. Homeostasis of cellular redox system is an imperative of cell surviving and its normal metabolism. ROS are well recognized for playing a dual role as both deleterious and beneficial species, since they can be either harmful or beneficial to living systems. These species are mutagenic compounds known to lead to DNA damage, favor cell transformation, and contribute to the development of a variety of malignant diseases. All the effects of oxidants are influenced by the cellular antioxidant defenses. This multilayer system consists of low molecular weight components and several antioxidant enzymes. Superoxide dismutases (SODs) are the only enzymes dismuting superoxide radicals. Mitomycin C, a cross-linking agent, demonstrated genotoxicity in all in vitro and in vivo test systems in mammalian cells and animals. Water-soluble fullerenes are well known as cytotoxic agents for many cell lines in vitro. At the other side, fullerenols are good free radical scavengers and antioxidants both in vitro and in vivo. This paper investigates the effects of fullerenol on survival and fullerenol/ /mytomicine (MMC) treatment on superoxide-dismutase (SOD) activity in CHO-K1 cells. Samples were treated 3 and 24 h with fullerenol (C60(OH)24) at concentration range 0.01-0.5 mg/mL and survival was monitored with dye exclusion test (DET). The activity of total SOD was estimated in samples treated with chosen concentrations of fullerenol and MMC (0.5 and 0.1 mg/mL) after 3 and 24 h of cell incubation. Increasing of C60(OH)24 concentration leads to decreasing of percent of surviving cells 3 and 24 h after incubation. The activity of total SOD enhanced with higher concentration of fullerenol, while decreased in the highest concentration at both experimental points. In samples treated with MMC, as well as in samples treated with fullerenol (0.0625 mg/mL) + MMC was noticed boost in total SOD activity in comparison with controls. Treatment with fullerenol decreased SOD activity in rest of samples treated with MMC. Decreased activity of superoxide-dismutase in almost all samples treated with fullerenol and MMC might be contributed to antioxidative properties of fullerenol. Increased enzyme level at concentration of 0.0625 mg/mL may be due to its prooxidative activity., U ovom radu ispitivani su efekti fulerenola (C60(OH)24) na preživljavanje, kao i tretmana fulerenolom i mitomicinom c (MMC) na aktivnost ukupne superoksid-dismutaze u CHO-K1 (ovarijalnih ćelija hrčka) ćelijskoj liniji. U uzorcima ćelija tretiranim fulerenolom koncentracija 0,01-0,5 mg/mL, praćeno je preživljavanje testom odbacivanja boje (DET) u 3 i 24 h tretmanu. Aktivnost superoksid-dismutaze (SOD) merena je u uzorcima tretiranim fulerenolom izabranih koncentracija i mitomicinom c (0,5 i 0,1 mg/mL) nakon 3 i 24 h. Sa porastom koncentracije (C60(OH)24) opada procenat preživelih ćelija tokom 3 i 24 h. Aktivnost SOD raste sa porastom koncentracije fulerenola i u najvećoj koncentraciji opada u obe vremenske tačke eksperimenta. U uzorcima tretiranim fulerenolom i MMC došlo je do smanjenja aktivnosti SOD, izuzev pri koncentraciji fulerenola od 0,0625 mg/mL, kada je zapažen porast aktivnosti SOD u odnosu na kontrolne grupe.",
journal = "Hemijska industrija",
title = "Aktivnost superoksid-dismutaze u animalnoj ćelijskoj kulturi CHO-K1 nakon tretmana fulerenolom i mitomicinom C, The activity of superoxide-dismutase in animal cell culture CHO-K1 after treatment with fullerenol and mytomicine C",
number = "3",
volume = "63",
pages = "143-149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_385"
}

Bogdanović, V., Slavić, M., Mrđanović, J., Šolajić, S.,& Đorđević, A.. (2009). Aktivnost superoksid-dismutaze u animalnoj ćelijskoj kulturi CHO-K1 nakon tretmana fulerenolom i mitomicinom C. in Hemijska industrija, 63(3), 143-149.
https://hdl.handle.net/21.15107/rcub_ibiss_385

Bogdanović V, Slavić M, Mrđanović J, Šolajić S, Đorđević A. Aktivnost superoksid-dismutaze u animalnoj ćelijskoj kulturi CHO-K1 nakon tretmana fulerenolom i mitomicinom C. in Hemijska industrija. 2009;63(3):143-149.
https://hdl.handle.net/21.15107/rcub_ibiss_385 .

Bogdanović, Višnja, Slavić, Marija, Mrđanović, Jasminka, Šolajić, Slavica, Đorđević, Aleksandar, "Aktivnost superoksid-dismutaze u animalnoj ćelijskoj kulturi CHO-K1 nakon tretmana fulerenolom i mitomicinom C" in Hemijska industrija, 63, no. 3 (2009):143-149,
https://hdl.handle.net/21.15107/rcub_ibiss_385 .

TY  - JOUR
AU  - Bogdanović, Višnja
AU  - Turšijan, Slavica
AU  - Đorđević, Milan
AU  - Nikolić, Aleksandra
AU  - Mrđanović, Jasminka
AU  - Jakimov, Dimitar
AU  - Trajković, Vesna
AU  - Bogdanović, Gordana
AU  - Ivković-Kapicl, Tatjana
AU  - Dugandžija, Tihomir
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/376
AB  - Background: A rapid increase in the turnover of malignant cells modulates the enzymes level in circulation and may be a prognostic sign of disease progression. We evaluated the activity of serum lactate dehydrogenase and superoxide dismutase in erythrocytes of breast cancer patients immediately after surgery and before adjuvant therapy. Methods: Fourteen newly operated breast cancer patients were included in the study. Lactate dehydrogenase and superoxide dismutase activity were estimated using standard tests for evaluation of enzyme activity. Results: Activity of lactate dehydrogenase was in normal range in all but one sample obtained from patient at clinical status IIIB. Superoxide dismutase activity was elevated in 11 out of 14 patients, and was 2 to 8 fold higher compare to control values. The highest activity of superoxide dismutase was found in samples of two patients at clinical status I. Conclusion: Determination of lactate dehydrogenase and superoxide dismutase activity might be useful in clinical follow up of breast cancer patients.
T2  - Archive of Oncology
T1  - Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma
IS  - 3-4
VL  - 16
SP  - 39
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_376
ER  - 

@article{
author = "Bogdanović, Višnja and Turšijan, Slavica and Đorđević, Milan and Nikolić, Aleksandra and Mrđanović, Jasminka and Jakimov, Dimitar and Trajković, Vesna and Bogdanović, Gordana and Ivković-Kapicl, Tatjana and Dugandžija, Tihomir",
year = "2008, 2008",
abstract = "Background: A rapid increase in the turnover of malignant cells modulates the enzymes level in circulation and may be a prognostic sign of disease progression. We evaluated the activity of serum lactate dehydrogenase and superoxide dismutase in erythrocytes of breast cancer patients immediately after surgery and before adjuvant therapy. Methods: Fourteen newly operated breast cancer patients were included in the study. Lactate dehydrogenase and superoxide dismutase activity were estimated using standard tests for evaluation of enzyme activity. Results: Activity of lactate dehydrogenase was in normal range in all but one sample obtained from patient at clinical status IIIB. Superoxide dismutase activity was elevated in 11 out of 14 patients, and was 2 to 8 fold higher compare to control values. The highest activity of superoxide dismutase was found in samples of two patients at clinical status I. Conclusion: Determination of lactate dehydrogenase and superoxide dismutase activity might be useful in clinical follow up of breast cancer patients.",
journal = "Archive of Oncology",
title = "Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma",
number = "3-4",
volume = "16",
pages = "39-41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_376"
}

Bogdanović, V., Turšijan, S., Đorđević, M., Nikolić, A., Mrđanović, J., Jakimov, D., Trajković, V., Bogdanović, G., Ivković-Kapicl, T.,& Dugandžija, T.. (2008). Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma. in Archive of Oncology, 16(3-4), 39-41.
https://hdl.handle.net/21.15107/rcub_ibiss_376

Bogdanović V, Turšijan S, Đorđević M, Nikolić A, Mrđanović J, Jakimov D, Trajković V, Bogdanović G, Ivković-Kapicl T, Dugandžija T. Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma. in Archive of Oncology. 2008;16(3-4):39-41.
https://hdl.handle.net/21.15107/rcub_ibiss_376 .

Bogdanović, Višnja, Turšijan, Slavica, Đorđević, Milan, Nikolić, Aleksandra, Mrđanović, Jasminka, Jakimov, Dimitar, Trajković, Vesna, Bogdanović, Gordana, Ivković-Kapicl, Tatjana, Dugandžija, Tihomir, "Activity of lactate dehydrogenase and superoxide dismutase in the circulation of patients with breast carcinoma" in Archive of Oncology, 16, no. 3-4 (2008):39-41,
https://hdl.handle.net/21.15107/rcub_ibiss_376 .