TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gašić, Uroš
AU  - Dabić Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
AU  - Fotirić Akšić, Milica
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/12/2822
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8706243
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4759
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.
IS  - 12
VL  - 10
DO  - 10.3390/plants10122822
SP  - 2822
ER  - 

@article{
author = "Skočajić, Dragana and Gašić, Uroš and Dabić Zagorac, Dragana and Nešić, Marija and Tešić, Živoslav and Meland, Mekjell and Fotirić Akšić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.",
number = "12",
volume = "10",
doi = "10.3390/plants10122822",
pages = "2822"
}

Skočajić, D., Gašić, U., Dabić Zagorac, D., Nešić, M., Tešić, Ž., Meland, M.,& Fotirić Akšić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland)
Basel: MDPI., 10(12), 2822.
https://doi.org/10.3390/plants10122822

Skočajić D, Gašić U, Dabić Zagorac D, Nešić M, Tešić Ž, Meland M, Fotirić Akšić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland). 2021;10(12):2822.
doi:10.3390/plants10122822 .

Skočajić, Dragana, Gašić, Uroš, Dabić Zagorac, Dragana, Nešić, Marija, Tešić, Živoslav, Meland, Mekjell, Fotirić Akšić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries." in Plants (Basel, Switzerland), 10, no. 12 (2021):2822,
https://doi.org/10.3390/plants10122822 . .

TY  - JOUR
AU  - Dabić Zagorac, Dragana Č.
AU  - Fotirić Akšić, Milica M.
AU  - Glavnik, Vesna
AU  - Gašić, Uroš
AU  - Vovk, Irena
AU  - Tešić, Živoslav Lj.
AU  - Natić, Maja M.
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201901271
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3622
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Wiley-VCH Verlag
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species
DO  - 10.1002/jssc.201901271
ER  - 

@article{
author = "Dabić Zagorac, Dragana Č. and Fotirić Akšić, Milica M. and Glavnik, Vesna and Gašić, Uroš and Vovk, Irena and Tešić, Živoslav Lj. and Natić, Maja M.",
year = "2020",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Wiley-VCH Verlag",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species",
doi = "10.1002/jssc.201901271"
}

Dabić Zagorac, D. Č., Fotirić Akšić, M. M., Glavnik, V., Gašić, U., Vovk, I., Tešić, Ž. Lj.,& Natić, M. M.. (2020). Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species. in Journal of Separation Science
Wiley-VCH Verlag..
https://doi.org/10.1002/jssc.201901271

Dabić Zagorac DČ, Fotirić Akšić MM, Glavnik V, Gašić U, Vovk I, Tešić ŽL, Natić MM. Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species. in Journal of Separation Science. 2020;.
doi:10.1002/jssc.201901271 .

Dabić Zagorac, Dragana Č., Fotirić Akšić, Milica M., Glavnik, Vesna, Gašić, Uroš, Vovk, Irena, Tešić, Živoslav Lj., Natić, Maja M., "Establishing the chromatographic fingerprints of flavan‐3‐ols and proanthocyanidins from rose hip ( Rosa sp.) species" in Journal of Separation Science (2020),
https://doi.org/10.1002/jssc.201901271 . .

TY  - JOUR
AU  - Dabić Zagorac, Dragana
AU  - Fotirić Akšić, Milica
AU  - Galvnik, Vesna
AU  - Gašić, Uroš
AU  - Vovk, Irena
AU  - Tešić, Živoslav
AU  - Natić, Maja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4066
AB  - The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
IS  - 8
VL  - 43
DO  - 10.1002/jssc.201901271
SP  - 1431
EP  - 1439
ER  - 

@article{
author = "Dabić Zagorac, Dragana and Fotirić Akšić, Milica and Galvnik, Vesna and Gašić, Uroš and Vovk, Irena and Tešić, Živoslav and Natić, Maja",
year = "2020",
abstract = "The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
number = "8",
volume = "43",
doi = "10.1002/jssc.201901271",
pages = "1431-1439"
}

Dabić Zagorac, D., Fotirić Akšić, M., Galvnik, V., Gašić, U., Vovk, I., Tešić, Ž.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271

Dabić Zagorac D, Fotirić Akšić M, Galvnik V, Gašić U, Vovk I, Tešić Ž, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .

Dabić Zagorac, Dragana, Fotirić Akšić, Milica, Galvnik, Vesna, Gašić, Uroš, Vovk, Irena, Tešić, Živoslav, Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .