TY  - JOUR
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - Schipper, Bert
AU  - Gagneul, David
AU  - Hendriks, Theo
AU  - Quillet, Marie-Christine
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S092666901831063X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3212
AB  - Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.
T2  - Industrial Crops and Products
T1  - Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)
VL  - 129
DO  - 10.1016/J.INDCROP.2018.12.011
SP  - 253
EP  - 260
ER  - 

@article{
author = "Bogdanović, Milica and Cankar, Katarina and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and Schipper, Bert and Gagneul, David and Hendriks, Theo and Quillet, Marie-Christine and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2019",
abstract = "Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.",
journal = "Industrial Crops and Products",
title = "Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)",
volume = "129",
doi = "10.1016/J.INDCROP.2018.12.011",
pages = "253-260"
}

Bogdanović, M., Cankar, K., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., Schipper, B., Gagneul, D., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2019). Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). in Industrial Crops and Products, 129, 253-260.
https://doi.org/10.1016/J.INDCROP.2018.12.011

Bogdanović M, Cankar K, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, Schipper B, Gagneul D, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). in Industrial Crops and Products. 2019;129:253-260.
doi:10.1016/J.INDCROP.2018.12.011 .

Bogdanović, Milica, Cankar, Katarina, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, Schipper, Bert, Gagneul, David, Hendriks, Theo, Quillet, Marie-Christine, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)" in Industrial Crops and Products, 129 (2019):253-260,
https://doi.org/10.1016/J.INDCROP.2018.12.011 . .

TY  - CONF
AU  - Cankar, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - van de Geest, Henri
AU  - Hendriks, Theo
AU  - Quillet, MC
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5441
AB  - Sesquiterpene lactones (STLs) give chicory its characteristic bitter taste and are highly concentrated in the latex channels of chicory taproot. The study of STLs is interesting to control the bitterness of industrial and consumption chicory, including witloof. The major STLs in chicory are lactucin, deoxylactucin, and lactucopicrin and oxalate and sulphate conjugates thereof. Formation of chicory STLs requires the biosynthesis of costunolide. Two germacrene A synthases, two germacrene A oxidases and a costunolide synthase have been described from chicory. In this work, the genetic organization of the genes involved in STL biosynthesis in chicory linkage groups is described. A number of genomic regions hosting STL biosynthetic genes have been addressed by NGS sequencing. Gene expression in different tissues is addressed by following their expression
using an RNAseq approach and by promoter activity studies using GFP. Finally, the downregulation of STL gene expression in chicory is achieved in hairy root culture regenerants expressing amiRNAs, and the consequences for STL content in different tissues is tested. These results will contribute to successful breeding strategies for chicory germplasm with well-defined STL content and bitterness.
PB  - Ghent : Phytochemical Society of Europe
C3  - Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium
T1  - Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5441
ER  - 

@conference{
author = "Cankar, Katarina and Bogdanović, Milica and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and van de Geest, Henri and Hendriks, Theo and Quillet, MC and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2016",
abstract = "Sesquiterpene lactones (STLs) give chicory its characteristic bitter taste and are highly concentrated in the latex channels of chicory taproot. The study of STLs is interesting to control the bitterness of industrial and consumption chicory, including witloof. The major STLs in chicory are lactucin, deoxylactucin, and lactucopicrin and oxalate and sulphate conjugates thereof. Formation of chicory STLs requires the biosynthesis of costunolide. Two germacrene A synthases, two germacrene A oxidases and a costunolide synthase have been described from chicory. In this work, the genetic organization of the genes involved in STL biosynthesis in chicory linkage groups is described. A number of genomic regions hosting STL biosynthetic genes have been addressed by NGS sequencing. Gene expression in different tissues is addressed by following their expression
using an RNAseq approach and by promoter activity studies using GFP. Finally, the downregulation of STL gene expression in chicory is achieved in hairy root culture regenerants expressing amiRNAs, and the consequences for STL content in different tissues is tested. These results will contribute to successful breeding strategies for chicory germplasm with well-defined STL content and bitterness.",
publisher = "Ghent : Phytochemical Society of Europe",
journal = "Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium",
title = "Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5441"
}

Cankar, K., Bogdanović, M., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., van de Geest, H., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2016). Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory. in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium
Ghent : Phytochemical Society of Europe., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_5441

Cankar K, Bogdanović M, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, van de Geest H, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory. in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium. 2016;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_5441 .

Cankar, Katarina, Bogdanović, Milica, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, van de Geest, Henri, Hendriks, Theo, Quillet, MC, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory" in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium (2016):65,
https://hdl.handle.net/21.15107/rcub_ibiss_5441 .

TY  - CONF
AU  - Cankar, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - van de Geest, Henri
AU  - Hendriks, Theo
AU  - Quillet, Marie-Christine
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6164
PB  - Vancouver: University of British Columbia
C3  - 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada
T1  - Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6164
ER  - 

@conference{
author = "Cankar, Katarina and Bogdanović, Milica and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and van de Geest, Henri and Hendriks, Theo and Quillet, Marie-Christine and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2015",
publisher = "Vancouver: University of British Columbia",
journal = "12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada",
title = "Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory",
pages = "102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6164"
}

Cankar, K., Bogdanović, M., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., van de Geest, H., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2015). Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory. in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada
Vancouver: University of British Columbia., 102.
https://hdl.handle.net/21.15107/rcub_ibiss_6164

Cankar K, Bogdanović M, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, van de Geest H, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory. in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada. 2015;:102.
https://hdl.handle.net/21.15107/rcub_ibiss_6164 .

Cankar, Katarina, Bogdanović, Milica, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, van de Geest, Henri, Hendriks, Theo, Quillet, Marie-Christine, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory" in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada (2015):102,
https://hdl.handle.net/21.15107/rcub_ibiss_6164 .