TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Plexida, Sofia
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2071-1050/12/20/8511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3943
AB  - The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.
PB  - MDPI AG
T2  - Sustainability
T1  - The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production
IS  - 20
VL  - 12
DO  - 10.3390/su12208511
SP  - 8511
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Plexida, Sofia and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production",
number = "20",
volume = "12",
doi = "10.3390/su12208511",
pages = "8511"
}

Petropoulos, S. A., Fernandes, Â., Plexida, S., Pereira, C., Dias, M. I., Calhelha, R., Chrysargyris, A., Tzortzakis, N., Petrović, J., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability
MDPI AG., 12(20), 8511.
https://doi.org/10.3390/su12208511

Petropoulos SA, Fernandes Â, Plexida S, Pereira C, Dias MI, Calhelha R, Chrysargyris A, Tzortzakis N, Petrović J, Soković M, Ferreira ICFR, Barros L. The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability. 2020;12(20):8511.
doi:10.3390/su12208511 .

Petropoulos, Spyridon A., Fernandes, Ângela, Plexida, Sofia, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production" in Sustainability, 12, no. 20 (2020):8511,
https://doi.org/10.3390/su12208511 . .

TY  - JOUR
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Petropoulos, Spyridon A.
AU  - Plexida, Sofia
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikos
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - C F R Ferreira, Isabel
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4494
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3577
AB  - In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244494
SP  - 4494
ER  - 

@article{
author = "Pereira, Carla and Dias, Maria Inês and Petropoulos, Spyridon A. and Plexida, Sofia and Chrysargyris, Antonios and Tzortzakis, Nikos and Calhelha, Ricardo C. and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Barros, Lillian and C F R Ferreira, Isabel",
year = "2019",
abstract = "In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).",
number = "24",
volume = "24",
doi = "10.3390/molecules24244494",
pages = "4494"
}

Pereira, C., Dias, M. I., Petropoulos, S. A., Plexida, S., Chrysargyris, A., Tzortzakis, N., Calhelha, R. C., Ivanov, M., Stojković, D., Soković, M., Barros, L.,& C F R Ferreira, I.. (2019). The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4494.
https://doi.org/10.3390/molecules24244494

Pereira C, Dias MI, Petropoulos SA, Plexida S, Chrysargyris A, Tzortzakis N, Calhelha RC, Ivanov M, Stojković D, Soković M, Barros L, C F R Ferreira I. The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland). 2019;24(24):4494.
doi:10.3390/molecules24244494 .

Pereira, Carla, Dias, Maria Inês, Petropoulos, Spyridon A., Plexida, Sofia, Chrysargyris, Antonios, Tzortzakis, Nikos, Calhelha, Ricardo C., Ivanov, Marija, Stojković, Dejan, Soković, Marina, Barros, Lillian, C F R Ferreira, Isabel, "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4494,
https://doi.org/10.3390/molecules24244494 . .