TY  - JOUR
AU  - Marković, Snežana D.
AU  - Đačić, Dragana S.
AU  - Cvetković, Danijela M.
AU  - Obradović, Ana D.
AU  - Žižić, Jovana B.
AU  - Ognjanović, Branka I.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/491
AB  - Ascorbic acid (Vit C) is a reducing agent that efficiently quenches potentially damaging free radicals. The aim of this study was to investigate effects of high acute dose of Vit C on redox and antioxidative status in blood of rats. Our study included two groups of Wistar albino rats: I - untreated group (control); II - group treated with Vit C (500 mg/kg bw, i.p.). In all cases animals were sacrificed after three days of treatment and blood samples were taken at the day of sacrifice. Concentration of Heinz bodies (HB) was determined in whole blood, while the concentration of reactive oxygen and nitrogen species (ROS and RNS), as well as, parameters of antioxidative defense system were followed in plasma and red blood cells (RBC). Our results showed that Vit C treatment induced higher levels of peroxynitrites and lipid peroxidation in plasma. In RBC, Vit C induced higher level of HB, GSSG/2 GSH ratio and glutathione peroxidase activity, while decreased glutathione reductase activity. In conclusion, acute high doses of Vit C act as prooxidant and induced oxidative stress that resulted in lipid peroxidation in plasma and hemoglobin oxidation in RBC. .
T2  - Kragujevac Journal of Science
T1  - Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats
IS  - 32
VL  - null
SP  - 109
EP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_491
ER  - 

@article{
author = "Marković, Snežana D. and Đačić, Dragana S. and Cvetković, Danijela M. and Obradović, Ana D. and Žižić, Jovana B. and Ognjanović, Branka I. and Štajn, Andraš Š. and Saičić, Zorica and Spasić, Mihajlo",
year = "2010, 2010",
abstract = "Ascorbic acid (Vit C) is a reducing agent that efficiently quenches potentially damaging free radicals. The aim of this study was to investigate effects of high acute dose of Vit C on redox and antioxidative status in blood of rats. Our study included two groups of Wistar albino rats: I - untreated group (control); II - group treated with Vit C (500 mg/kg bw, i.p.). In all cases animals were sacrificed after three days of treatment and blood samples were taken at the day of sacrifice. Concentration of Heinz bodies (HB) was determined in whole blood, while the concentration of reactive oxygen and nitrogen species (ROS and RNS), as well as, parameters of antioxidative defense system were followed in plasma and red blood cells (RBC). Our results showed that Vit C treatment induced higher levels of peroxynitrites and lipid peroxidation in plasma. In RBC, Vit C induced higher level of HB, GSSG/2 GSH ratio and glutathione peroxidase activity, while decreased glutathione reductase activity. In conclusion, acute high doses of Vit C act as prooxidant and induced oxidative stress that resulted in lipid peroxidation in plasma and hemoglobin oxidation in RBC. .",
journal = "Kragujevac Journal of Science",
title = "Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats",
number = "32",
volume = "null",
pages = "109-116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_491"
}

Marković, S. D., Đačić, D. S., Cvetković, D. M., Obradović, A. D., Žižić, J. B., Ognjanović, B. I., Štajn, A. Š., Saičić, Z.,& Spasić, M.. (2010). Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats. in Kragujevac Journal of Science, null(32), 109-116.
https://hdl.handle.net/21.15107/rcub_ibiss_491

Marković SD, Đačić DS, Cvetković DM, Obradović AD, Žižić JB, Ognjanović BI, Štajn AŠ, Saičić Z, Spasić M. Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats. in Kragujevac Journal of Science. 2010;null(32):109-116.
https://hdl.handle.net/21.15107/rcub_ibiss_491 .

Marković, Snežana D., Đačić, Dragana S., Cvetković, Danijela M., Obradović, Ana D., Žižić, Jovana B., Ognjanović, Branka I., Štajn, Andraš Š., Saičić, Zorica, Spasić, Mihajlo, "Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats" in Kragujevac Journal of Science, null, no. 32 (2010):109-116,
https://hdl.handle.net/21.15107/rcub_ibiss_491 .

TY  - JOUR
AU  - Marković, Snežana D.
AU  - Obradović, Ana D.
AU  - Žižić, Jovana B.
AU  - Ognjanović, Branka I.
AU  - Štajn, A.Š.
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/262
AB  - In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.
AB  - Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim ćelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viši nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je više koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemične grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.
T2  - Archives of Biological Sciences
T1  - Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem
T1  - Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding
IS  - 3
VL  - 62
SP  - 589
EP  - 594
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_262
ER  - 

@article{
author = "Marković, Snežana D. and Obradović, Ana D. and Žižić, Jovana B. and Ognjanović, Branka I. and Štajn, A.Š. and Saičić, Zorica and Spasić, Mihajlo",
year = "2010, 2010",
abstract = "In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma., Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim ćelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viši nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je više koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemične grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.",
journal = "Archives of Biological Sciences",
title = "Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem, Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding",
number = "3",
volume = "62",
pages = "589-594",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_262"
}

Marković, S. D., Obradović, A. D., Žižić, J. B., Ognjanović, B. I., Štajn, A.Š., Saičić, Z.,& Spasić, M.. (2010). Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem. in Archives of Biological Sciences, 62(3), 589-594.
https://hdl.handle.net/21.15107/rcub_ibiss_262

Marković SD, Obradović AD, Žižić JB, Ognjanović BI, Štajn A, Saičić Z, Spasić M. Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem. in Archives of Biological Sciences. 2010;62(3):589-594.
https://hdl.handle.net/21.15107/rcub_ibiss_262 .

Marković, Snežana D., Obradović, Ana D., Žižić, Jovana B., Ognjanović, Branka I., Štajn, A.Š., Saičić, Zorica, Spasić, Mihajlo, "Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem" in Archives of Biological Sciences, 62, no. 3 (2010):589-594,
https://hdl.handle.net/21.15107/rcub_ibiss_262 .