TY  - CHAP
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Stanisavljević, Danijela
AU  - Ivanov, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2019
UR  - https://www.eurekaselect.com/176849/chapter/cytotoxicity-through-molecular-targets-involved-in-apoptosis.-where-should-we-further-search-for-mushrooms-functi
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3964
AB  - Apoptosis is considered as a classical way of programmed cell death and important control mechanism of cell homeostasis. Cancer cells acquire different instruments to circumvent programmed cell death. This promotes uncontrolled growth and frequently confers chemoresistance to tumor cells. Activation of apoptotic signaling pathway has been a target of anti-cancer drugs in an induction of cytotoxicity. The mechanism of apoptosis is complex and includes many pathways. This chapter will focus on the current knowledge of apoptosis-triggering approaches in cancer therapy, as well as on mushroom extracts and isolated compounds acting as initiators of apoptosis through regulation of genes expression involved in cancer cell death. Furthermore, we pointed out directions for novel search for mushroom functionalities in this experimental field and discuss the possible further steps for exploration on the in vitro and in vivo levels.
PB  - Bentham Science Publishers
T2  - Frontiers in Natural Product Chemistry
T1  - Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?
VL  - 5
DO  - 10.2174/9789811423772119050007
SP  - 146
EP  - 191
ER  - 

@inbook{
editor = "Atta-ur-Rahman",
author = "Stojković, Dejan and Živković, Jelena and Stanisavljević, Danijela and Ivanov, Marija and Popović, Jelena and Stevanović, Milena and Glamočlija, Jasmina and Soković, Marina",
year = "2019",
abstract = "Apoptosis is considered as a classical way of programmed cell death and important control mechanism of cell homeostasis. Cancer cells acquire different instruments to circumvent programmed cell death. This promotes uncontrolled growth and frequently confers chemoresistance to tumor cells. Activation of apoptotic signaling pathway has been a target of anti-cancer drugs in an induction of cytotoxicity. The mechanism of apoptosis is complex and includes many pathways. This chapter will focus on the current knowledge of apoptosis-triggering approaches in cancer therapy, as well as on mushroom extracts and isolated compounds acting as initiators of apoptosis through regulation of genes expression involved in cancer cell death. Furthermore, we pointed out directions for novel search for mushroom functionalities in this experimental field and discuss the possible further steps for exploration on the in vitro and in vivo levels.",
publisher = "Bentham Science Publishers",
journal = "Frontiers in Natural Product Chemistry",
booktitle = "Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?",
volume = "5",
doi = "10.2174/9789811423772119050007",
pages = "146-191"
}

Atta-ur-Rahman, Stojković, D., Živković, J., Stanisavljević, D., Ivanov, M., Popović, J., Stevanović, M., Glamočlija, J.,& Soković, M.. (2019). Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?. in Frontiers in Natural Product Chemistry
Bentham Science Publishers., 5, 146-191.
https://doi.org/10.2174/9789811423772119050007

Atta-ur-Rahman, Stojković D, Živković J, Stanisavljević D, Ivanov M, Popović J, Stevanović M, Glamočlija J, Soković M. Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?. in Frontiers in Natural Product Chemistry. 2019;5:146-191.
doi:10.2174/9789811423772119050007 .

Atta-ur-Rahman, Stojković, Dejan, Živković, Jelena, Stanisavljević, Danijela, Ivanov, Marija, Popović, Jelena, Stevanović, Milena, Glamočlija, Jasmina, Soković, Marina, "Cytotoxicity Through Molecular Targets Involved in Apoptosis. Where Should We Further Search for Mushrooms Functionalities in Future Cancer Treatment?" in Frontiers in Natural Product Chemistry, 5 (2019):146-191,
https://doi.org/10.2174/9789811423772119050007 . .

TY  - JOUR
AU  - Aleksić, Milena
AU  - Stanisavljević, Danijela
AU  - Ivanov, Marija
AU  - Vasiljević, Perica
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Stojković, Dejan
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12532
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3444
AB  - Pyrimethanil, a synthetic fungicide widely used for the treatment of pre‐ and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 μg μL−1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng μL−1, while the expression of Ki67 marker of proliferation was decreased with 150 ng μL−1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.
T2  - Annals of Applied Biology
T1  - Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
IS  - 2
VL  - 175
DO  - 10.1111/aab.12532
SP  - 228
EP  - 235
ER  - 

@article{
author = "Aleksić, Milena and Stanisavljević, Danijela and Ivanov, Marija and Vasiljević, Perica and Stevanović, Milena and Soković, Marina and Stojković, Dejan",
year = "2019",
abstract = "Pyrimethanil, a synthetic fungicide widely used for the treatment of pre‐ and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 μg μL−1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng μL−1, while the expression of Ki67 marker of proliferation was decreased with 150 ng μL−1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.",
journal = "Annals of Applied Biology",
title = "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines",
number = "2",
volume = "175",
doi = "10.1111/aab.12532",
pages = "228-235"
}

Aleksić, M., Stanisavljević, D., Ivanov, M., Vasiljević, P., Stevanović, M., Soković, M.,& Stojković, D.. (2019). Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology, 175(2), 228-235.
https://doi.org/10.1111/aab.12532

Aleksić M, Stanisavljević D, Ivanov M, Vasiljević P, Stevanović M, Soković M, Stojković D. Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology. 2019;175(2):228-235.
doi:10.1111/aab.12532 .

Aleksić, Milena, Stanisavljević, Danijela, Ivanov, Marija, Vasiljević, Perica, Stevanović, Milena, Soković, Marina, Stojković, Dejan, "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines" in Annals of Applied Biology, 175, no. 2 (2019):228-235,
https://doi.org/10.1111/aab.12532 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Stanisavljević, Danijela
AU  - Stojković, Dejan
AU  - Petrović, Isidora
AU  - Vićentić Marjanović, Jelena
AU  - Popović, Jelena
AU  - Golić Grdadolnik, Simona
AU  - Marković, Dejan
AU  - Sanković-Babić, Snežana
AU  - Glamočlija, Jasmina
AU  - Stevanović, Milena
AU  - Soković, Marina
PY  - 2017
UR  - http://www.excli.de/vol16/Sokovic_23052017_proof.pdf
UR  - http://www.excli.de/volume16.php
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2791
AB  - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell’s viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
T2  - EXCLI Journal
T1  - Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions
VL  - 16
DO  - 10.17179/excli2017-300
SP  - 795
EP  - 807
ER  - 

@article{
author = "Ivanov, Marija and Stanisavljević, Danijela and Stojković, Dejan and Petrović, Isidora and Vićentić Marjanović, Jelena and Popović, Jelena and Golić Grdadolnik, Simona and Marković, Dejan and Sanković-Babić, Snežana and Glamočlija, Jasmina and Stevanović, Milena and Soković, Marina",
year = "2017",
abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell’s viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.",
journal = "EXCLI Journal",
title = "Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions",
volume = "16",
doi = "10.17179/excli2017-300",
pages = "795-807"
}

Ivanov, M., Stanisavljević, D., Stojković, D., Petrović, I., Vićentić Marjanović, J., Popović, J., Golić Grdadolnik, S., Marković, D., Sanković-Babić, S., Glamočlija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal, 16, 795-807.
https://doi.org/10.17179/excli2017-300

Ivanov M, Stanisavljević D, Stojković D, Petrović I, Vićentić Marjanović J, Popović J, Golić Grdadolnik S, Marković D, Sanković-Babić S, Glamočlija J, Stevanović M, Soković M. Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807.
doi:10.17179/excli2017-300 .

Ivanov, Marija, Stanisavljević, Danijela, Stojković, Dejan, Petrović, Isidora, Vićentić Marjanović, Jelena, Popović, Jelena, Golić Grdadolnik, Simona, Marković, Dejan, Sanković-Babić, Snežana, Glamočlija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807,
https://doi.org/10.17179/excli2017-300 . .