TY  - JOUR
AU  - Stupar, Petar
AU  - Podolski-Renić, Ana
AU  - Villalba, Maria Ines
AU  - Dragoj, Miodrag
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Kasas, Sandor
PY  - 2021
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147836/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4247
AB  - Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer world-wide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.
PB  - MDPI AG
T2  - Medicina
T1  - Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
IS  - 5
VL  - 57
DO  - 10.3390/medicina57050446
SP  - 446
ER  - 

@article{
author = "Stupar, Petar and Podolski-Renić, Ana and Villalba, Maria Ines and Dragoj, Miodrag and Jovanović Stojanov, Sofija and Pešić, Milica and Kasas, Sandor",
year = "2021",
abstract = "Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer world-wide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.",
publisher = "MDPI AG",
journal = "Medicina",
title = "Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics",
number = "5",
volume = "57",
doi = "10.3390/medicina57050446",
pages = "446"
}

Stupar, P., Podolski-Renić, A., Villalba, M. I., Dragoj, M., Jovanović Stojanov, S., Pešić, M.,& Kasas, S.. (2021). Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics. in Medicina
MDPI AG., 57(5), 446.
https://doi.org/10.3390/medicina57050446

Stupar P, Podolski-Renić A, Villalba MI, Dragoj M, Jovanović Stojanov S, Pešić M, Kasas S. Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics. in Medicina. 2021;57(5):446.
doi:10.3390/medicina57050446 .

Stupar, Petar, Podolski-Renić, Ana, Villalba, Maria Ines, Dragoj, Miodrag, Jovanović Stojanov, Sofija, Pešić, Milica, Kasas, Sandor, "Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics" in Medicina, 57, no. 5 (2021):446,
https://doi.org/10.3390/medicina57050446 . .